Sphingosine analogue FTY720 exerts good immune suppressive effect by inducing lymphocytes apoptosis and homing, which can be used for organ transplant rejection as well as anti-autoimmune disease treatment. Recent studies have found that FTY720 plays anti-tumor potential by inducing growth arrest and apoptosis as well as inhibiting tumor metastasis.

Hypoxia- inducible factor-1 ( HIF-1) promotes tumor deterioration, invasion and metasta-sis. Treatment targeting the HIF-1 signal pathway is becoming a hot research. In addition to uniquely selective inhibition of topoisomerase Ⅰ(Topo Ⅰ) ,the recent studies show that camptothecin compounds can regulate the activity of HIF-1 through decreasing HIF-1 protein translation and have been classified as a non-selective chemical inhibitor of HIF-1.

[Objective] Sorting and summing up Professor ZHANG 's clinical experience in the diagnosis and treatment of atlanto-axial joint subluxation, to develop the thought of clinical diagnosis and treatment of the disease. [Methods]Via studying with professor ZHANG, and collectting some cases,consulting the related literature and data.Finally, discuss and summarize it. [Results] Diagnosis found and appliedFour tenderness(Below the foramen magnum, trapezius muscle and at the junction of the skull,fengchi point,wangu point).Method combined with primary symptom and signs, X-ray. Treatment, application of error correction manipulation, light effect;And with local acupuncture point injection, menstruation invigorates the circulation of reinforcement. [Conclusion] Professor ZHANG's method of diagnosis and treatment of atlanto-axial joint subluxation is unique,clear, simple, has obvious curative effect, worth clinical application and promotion.

AIM:To establish a method for determining notoginsenoside R_1,ginsenoside Rg_1 and ginsenoside Rb_1 in Jingkang Capsules(Radix Rehmanniae praeparata,Radix Polygoni multiflori,Radix et Rhizoma Notoginseng,etc.). METHODS: A Diamonsil C_(18) column(250 mm?4.6 mm,5 ?m) with acetonitrile-water as the mobile phase was used for gradient elution,the flow rate was 1.0 mL/min and the UV detection wavelength was at 203 mn. RESULTS: There was a good linear relationship at a range of 0.327-2.180 ?g for notoginsenoside R_1(r=(0.999 4))(the average recovery was 98.23% with RSD of 1.53%(n=6)),0.909-6.060 ?g for ginsenoside Rg_1(r=1.0)(the average recovery was 99.04% with RSD of 1.00%(n=6)),0.672-4.480 ?g for ginsenoside Rb_1(r=0.999 8)(the average recovery was 98.17% with RSD of 1.31%(n=6)).CONCLUSION: The method is simple,quick and accurate.It can be used for the quality control of Jingkang Capsules.

Currently the most effective treatment strategy for breast cancer is standardized multi-discipline comprehensive treatment. However, there are no effective models that can accurately predict prognosis, so that no guidance of individualized treatment has yet been set up, resulting in a proportion of patients with low risk who received chemotherapy with little benefit. With the development of genomics, several gene sets have been demonstrated to be helpful in predicting of breast cancer prognosis and grading the patients' benefit from chemotherapy, thus avoid overtreatment. 21-gene Oncotype Dx was reported as one of them and has been demonstrated to be effective and accurate in various clinical studies. This paper summarizes researches on 21-gene Oncotype Dx in breast cancer.

Cystatin C (Cys C) is used to evaluate the glomerular filtration rate (GFR), renal function and thus reflects changes in endogenous marker. Cys C is not affected by gender, muscle mass, malignancy and its production rate is usually constant, making it one of ideal markes of GFR. This article focuses on clinical application and research of Cys C in a variety of renal diseases, especially in renal dysfunction at an early stage of clinical significance.

Objective To observe the expressions of c-Ski in renal tissue of diabetic nephropathy rats, and discuss its significance. Methods Wistar male rats were randomly assigned to 3 groups: Control group, diabetic group and treatment group. Rats were killed at the 16th week after experiment. Histopathologic changes,in renal tissue were observed and immunohistochemistry was performed to investigate the expression of TGF-β1, α-SMA and c-Ski. Results The expression of c-Ski had a negative correlation with TGF-β1 and α-SMA. Compared to diabetic group, the expression of c-Ski was significantly increased in treatment group. Conclusion c-Ski may be a protective factor of tu-bular epithelial-myofibroblast transformation.

Objective To explore the effect of the Yu-tone music to reduction of fear emotion. Methods 72 undergraduate students listened to Yu-tone Music or had a rest for ten minutes after elicitation of fear emotion by film clip;and measured from autonomic nerves system include skin conduction,BVP amplitude,heart rate,respiration,finger temperature and self-report subjective experience. Result 1.Compared with silence[(0.96±1.64)1/Ω,(0.85±1.73)min-1,(4.48±43.54)μV,(-5±15.37)min-1],music was associated with a significantly decreased skin resistance,respiration rate,heart rate and myoelectricity after fear emotion[(2.04±2.22)1/Ω,(0.09±1.79)min-1,(8.47±26.24) μV,(1.57±20.88) min-1,P <0.01,P <0.01,P <0.01,P <0.05]; 2.Compared with silence[(-0.37±2.71)μV, (0.13±1.16) ℃ ],music was enlarged BVP amplitude and skin temperature[(-0.53±3.8)μV,(-0.02±0.87)℃],but there was no significant difference( P ＞0.05,P ＞0.05).Conclusion Music advance the restore of negative emotion,and reduce excitability of the sympathetic and improve function of the parasympathetic nervous systems. Simultaneously,music significantly enhance the feelings of happiness as well as quietness of positive emotion.

ObjectiveTo explore the relationship of organizational factors with occupational burnout among doctors. Method740 doctors were assessed by CMBI, Job Demands and Decision Latitude scale, Distributive and Procedural Justice scale, Cross-Culture Role Conflict, Ambiguity, and Overload scale, Work Interference With Family and Family Interference With Work scale, Social Support scale. ResultsHierarchical multiple regression indicated that 27.8% variance of doctors' Emotional Exhaustion(EE), 36.8% variance of Depersonalization(Dp), 21.0% variance of Reduced Personal Accomplishment(RPA) were explained by Organizational factors. Work Interference with family, role conflict, work-family conflict, distributive justice and workload can significantly predict EE(Standardized β was 0.204, 0.102, 0.249,-0.109,0.093); Family interference with work, role ambiguity, family support, procedural justice and workload can significantly predict Dp(Standardized β was 0.506,0.192,-0.122,0.105,-0.068); Role ambiguity, family support, job control, work Interference with family and superior support can significantly predict RPA(Standardized β was 0.245,-0.179,-0.172,-0.106,-0.069). ConclusionOrganizational factors have significantly effect on doctor's burnout, especially work-family conflict and role character.

Objective To acquaint the condition of learning burnout of medical students and to explore the relationship of learning burnout with professional commitment and social support. Methods 610 medical students were evaluated with Learning Burnout Scale, professional commitment scale and social support scale. Results ①In general, learning burnout index of medical students was 2.84±0.49; in terms of gender difference, male students [(2.83±0.65) and (3.08±0.61)] were higher than females[(2.70±0.63) and (2.96±0.60)] at the level of emotional turndown and behavioral unsuitableness, the difference was statistically significant( P <0.05); in terms of grades, medical students'learning burnout of different grades was significant( F =4.244,P <0.01); there was no difference in profession. ②Learning burnout was significantly negative correlation with professional commitment and social support( P <0.01). ③Multiple regression analysis showed that affective commitment and family support had significant predications on learning burnout of medical students( P <0.01). Conclusion Medical students'learning burnout level is comparatively high, learning burnout closely relates to professional commitment and social support.