Objective To investigate the damage of mice liver and kidney with the compatibility application of Paeoniae radix rubra and Veratrum nigrum, and explore its mechanism. Methods We examined the alanine aminotransferase, aspartate aminotransferase (AST), lactic dehydrogenase, creatinine and urea nitrogen from serum, and glutathion peroxidase (GSH-Px) and malondialdehyde (MDA) from liver and kidney tissue after intragastric administration of different ratio (4∶1, 2∶1, 1∶1, 1∶2, 1∶4) of Paeoniae radix rubra and Veratrum nigrum in mice for 22 d. Results Paeoniae radix rubra used with Veratrum nigrum did not induce the obvious damage to liver with AST increase, but induced the obvious damage to kidney. At the same time, the depressed GSH-Px activity and the increased MDA content were observed in kidney tissue. When Paeoniae radix rubra and Veratrum nigrum is on a ratio of 2∶1, the kidney injury was the most obvious. Conclusion Paeoniae radix rubra used with Veratrum nigrum in varying proportions could induce the kidney injury in mice which is related to the oxidation-antioxidation balance disturbance.

Objective To investigate the anti-HBV mechanism of oxymatrine in vitro.Methods The antiviral mechanism of oxymatrine was analyzed through detecting the expression and function of TLR9 signal pathway and the secretion of antiviral cytokines from PBMCs treated by oxymatrine in vitro.Results Oxymatrine could not only induce IFN-α and IFN-γ secretions from PBMCs directly, but also effectively augmented the expressions of TLR9, MyD88 and TRAF6 at mRNA level, and activated the TLR9 signal transduction pathway. TLR9 ligand could induce IFN-α, IFN-γ and TNF-α secretion a lot from PBMCs pretreated by oxymatrine.Conclusion Oxymatrine possesses an anti-HBV activity via activation of TLR9 signal pathway and secretion of antiviral cytokines.

Objective To explore the role of receptor-interaction proteins(RIP) in phenethylisothiocyanate(PEITC)-induced apoptosis in human leukemia cells.Methods U937 cells were exposed to PEITC at various concentrations of 0,2,4,6 and 8 ?mol/L for 3 and 6 h,or at 8 ?mol/L for different time intervals of 1,3,6,9,12 and 24 h.Cells were stained with Annexin V/PI,and apoptosis was determined by using flow cytometry.Total protein extracts were prepared and subjected to Western blot assay using antibodies against poly(ADP-ribose)polymerase(PARP),Caspase-3,Caspase-8,and RIP.?-actin was used to ensure equivalent loading.Results After exposure to PEITC at concentrations of 2,4,6 and 8 ?mol/L for 3 h,U937 cells showed an increase in cell apoptosis(9% ,25% ,44% and 56% respectively).While when the time expanded to 6 h,the amount of apoptotic cells was increased to 16% ,28% ,51% and 82% respectively.A dose-dependent manner was seen when U937 cells were exposure to PEITC at different concentrations.When U937 cells were treated by PEITC at concentration of 8 ?mol/L for 1,3,6,9,12,and 24 h,the apoptotic cells covered 12% ,57% ,79% ,86% ,90% ,and 91% of all cells respectively,indicating a time-dependent manner.Western blot assay showed that PEITC caused the activation/cleavage of Caspase-3 and-8,and improved the PARP cleavage,and also decreased the expression of RIP.PEITC-induced apoptosis was proceeded by degradation of RIP.Conclusion Our results indicate that PEITC induces the apoptosis in human leukemia cells through a process that involves death receptor pathway,and RIP may play an important role in the apoptosis.

Objective To investigate the protective function of oxymatrine on the renal intestitial fibrosis in rats.Methods Forty male Sprague Dawley rats were randomly divided into 5 groups: sham group,model group,Lotensin group,large-dose oxymatrine group,and small-dose oxymatrine group.The models were established by unilateral ureteral obstruction(UUO) of the left side.On the 14~(th) day after operation,the obstructed kidney was taken out.Then,HE,Massion,and immunohistochemistry staining of transforming growth factor-?_(1)(TGF-?_(1)),(?-smooth) muscle acting(?-SMA) and collagen Ⅲ were conducted.After that,semiquantitative analysis was performed.Results After oxymatrine,treatment,the expressions of ?-SMA,TGF-?_(1) and ColⅢ of the obstructed kidney in the treatment group were significantly lower than those in the model group(P0.05).Conclusion Oxymatrine may reduce the expression of cytokines such as TGF-?_(1),then the activation of cell producing ECM and then the sendimentation of ECM,thus preventing renal interstitial fibrosis.

Objective To study the effect of Benazepril on the renal connective tissue growth factor(CTGF) expression and interstitial fibrosis in unilateral ureteral obstruction(UUO) rats and to illuminate the possible mechanisms.Methods 24 Sprague-Dawley rats were randomly divided into Sham-operated,control and Benazepril groups.From the day before operation,the rats were under intragastric administration of Benazepril 10mg/(kg?d) in Benazepril group,and sodium chloride in tales doses in Sham and control groups.On the 14~(th) day after operation,the obstructed kidney was taken out to be measured by HE,Masson,and immunohistochemistry staining for TGF-?_1,CTGF,?-SMA and ColⅢ.Results The score of renal interstitial lesion and fibrosis index in Benazenpril were significantly lower than those in the control group(P

OBJECTIVE:To study the stability of Bovine Lactoferrin.METHODS:The content of Bovine Lactoferrin and its hybridprotein were determined by RP-HPLC.The stability of the samples was tested by high-light exposure,accelerating experiment and room temperature storage observation method.RESULTS:The linear range for Bovine Lactoferrin was25～ 1000?g/ml,RSD=0.05%.Under the high-light exposure experiment,accelerating experiment and room temperature storage observation,all the criteria were found to be in line with the quality standard specification.CONCLUSION:Stored under room temperature and airtight condition,Bovine Lactoferrin is stable in quality.

Objective To study the effect of pentoxifylline (PTX) on acute lung injury (ALI) in murine sepsis induced by intra abdominal infection. Methods Wistar rats were subjected to sepsis caused by cecal ligation and puncture (CLP), and animals were randomly divided into normal controls ( n =6), sepsis group ( n =18), and PTX treated group ( n =18). Pulmonary biopterin, nitric oxide (NO) levels, and pulmonary myeloperoxidase (MPO) activities were measured, guanosine triphosphate cyclohydrolase Ⅰ (GTP CHI), inducible nitric oxide synthase (iNOS), and tumor necrosis factor ? (TNF ?) mRNA expression were determined.Results Early treatment with PTX significantly decreased pulmonary MPO activities and TNF ?mRNA expression at 2 hours after CLP. Meanwhile, pulmonary biopterin, NO, GTP CHI, and iNOS mRNA expression levels were much lower in the treatment group than those in the non treatment group ( P

AIM: To conclude the characteristics of flash visual evoke potentials (FVEP), and pattern visual evoke potentials (PVEP) of the healthy school-age children.And to compare the two methods, in order to find the association of them, and to find the impact of sex, age, and the other biological variables.METHODS: A total of 101 healthy children were recruited (age from 5 to 14.4y, mean 8.27y).Each of them was underwent FVEP and PVEP examinations.Then the results were statistically analyzed by SPSS 13.0.RESULTS: The curves of PVEP are simple and stable, while FVEP waveforms are variable.The latency of P100 of females is shorter than males.However there was no significant difference for FVEP in sex control.To compare the parameters between the two hemispheres, the amplitude of P100 of left eyes were higher than the right side.FVEP showed no difference in the two hemispheres either.There was no significant difference for age-dependent decreased in neither PVEP nor FVEP.And in a regression analysis of the FVEP and PVEP, we could not find the inner connection of the two methods.CONCLUSION: Based on our research, there were no significant differences in age level or sex control in the period of school-age children.And there is no inner connection of the two methods.The differences between the PVEP and FVEP results might be due to the origin of these two responses.And these two stimuli should be used in a complementary manner not as alternative examinations.

Objective To investigate the risks of HBV infection among the spouses of HBV surface antigen (HBsAg)carriers and to find out effective control strategies on hepatitis B control and prevention .Methods To use case-control study(1∶2) including spouses of HBsAg carriers aged 18 -59 years-old from the nationwide sero-epidemiological survey for Hepatitis B in Chonqing province in 2005 ,and the spouses of the healthy(HBsAg negative persons)as the control groups ,adopt euzymelinked immunosor-bent assay(ELISA) to carry out the sero-epidemiological testing (HBsAg ,anti-HBc ,anti-HBs) for Hepatitis B .Results The posi-tive rate of HBsAgamong the spouses of HBsAg carriers (14 .8% ) was higher than the rate of spouse among the healthy (7 .5% ) , with difference statistically significant (P<0 .01) ,the positive rate of HBsAg in female spouse was uptrend as the marriage age grown ,multiple factor analysis found that the risks of HBV infection among the spouses were their spouse with HBsAg and without condom when the sexual behavior .Conclusion The spouses of HBsAg carriers have high risks of HBV .Premarital examinations , safety sexual behaviors with condom ,timely hepatitis B vaccination etc .are helpful for the spouses of HBsAg carriers free of hepati-tis B virus ,especially female spouses should insist on using condom when the sexual behavior because of them easier to be infected by the hepatitis B virus .

Objective To discuss the effectiveness of heart operation through subaxillary minithoracotomy.Methods Subaxillary minithoracotomy heart operation was carried out in 378 cases between March 1995 and October 2004.The operation was performed under beating heart and without the occlusion of the ascending aorta in 81 cases of atrial septal defect, 32 cases of ventricular septal defect,and 16 cases of other diseases.The operation was completed under low temperature and extracorporeal circulation in other 249 cases.Results The operation was accomplished smoothly in all the cases.The operation time was 167?2.0 min,and the extracorporeal circulation time was 28.1?16.6 min.Postoperatively,1 patient with double-chamber anormaly of the right ventricle was given a tracheotomy because of an acute laryngeal edema,1 patient with tetralogy of Fallot died of low cardiac output syndrome,1 patient had a cerebral embolism,2 patients experienced an incision fat liquefaction,and the remaining patients recovered uneventfully.A follow-up for 2 months ~ 10 years(mean,5.1 years) in 366 patients revealed a full range of physical activities.One case of ventricular septal defect with residual shunt and 1 case of cerebral embolism were fully recovered.Conclusions Subaxillary minithoracotomy open heart operation has advantages of minimal invasion,quick recovery,simplicity of performance,and good cosmetic results.