OBJECTIVETo study the effect of water content in soil on physiological characters and yield of Artemisia annua.

METHODThe pot experiment was applied and activity of protective enzyme, biomass and artemisinin accumulation were measured under different water treatments.

RESULT AND CONCLUSIONThe results showed that contents of osmotic adjustable substances, activity of protective enzyme, biomass and artemisinin accumulation were greatly affected by water content in the soil. Under water stress the water content in leave decreased, relative plasmalemma permeability increased, proline quickly accumulated to promote water retaining capability of cell, POD, CAT and SOD cooperated to reduce lipid peroxidation and reduced cell damage, and biomass decreased. At the seedling stage, the content of artemisinin and yield reached the maximal when the water content in soil was between 50%-55%. At the beginning of the branching stage, the content of artemisinin was the highest at the water content of 50%-55%, while the yield reached the maximal at the water content of 70%-75%. At the end of branching stage, the content of artemisinin was the highest at the water content of 40%-45%, while the yield reached the maximal at the water content of 60%-65%. In conclusion, the optimum water content in soil was between 50%-55% at the seedling stage, at the branching stage, higher water content was beneficial for the higher yield.

Antimalarials ; analysis ; pharmacology ; Artemisia annua ; chemistry ; physiology ; Artemisinins ; analysis ; Biomass ; Plant Leaves ; chemistry ; Plant Transpiration ; Seedlings ; Soil ; analysis ; Water ; Water Movements

OBJECTIVETo study the genetic diversity of Codonopsis tangshen.

METHODEighteen germplasmic resources of C. tangshen were analyzed by SRAP and ISSR molecular markers. The systematic diagram of genetic relationship was made by TREECONW software and clustered by UPGMA method.

RESULTTwenty-nine SRAP primer combination amplified 329 bands with 266 (80.85%) polymorphic and 21 ISSR primers amplified 223 bands with 166 (74.44%) polymorphic. The average genetic similarity coefficient was 0.7121 (by SRAPs) and 0.7781 (by ISSRs). Both SRAP and ISSR analyses revealed a high level of genetic diversity in C. tangshen. By cluster analysis, the geographical distribution was not distinctive. The significant positive correlation between SRAPs and ISSRs was observed (r=0.802, P<0.01), although the dendrograms based on SRAP and ISSR markers were not all the same.

CONCLUSIONDifferent germplasms diversity of C. tangshen is high and SRAP and ISSR can be used in genetic diversity study of C. tangshen.

Algorithms ; Codonopsis ; genetics ; DNA, Plant ; analysis ; Genetic Markers ; genetics ; Genetic Variation ; Genotype ; Phylogeny ; Polymorphism, Genetic ; Sequence Homology, Nucleic Acid