1.Metabolism of mangiferin by human intestinal bacteria in vitro.
Huixue HUANG ; Zhenyuan TAN ; Jiagang DENG ; Qiuyun LIANG ; Yumei NONG ; Nianmei SONG
China Journal of Chinese Materia Medica 2011;36(4):443-445
OBJECTIVETo study the metabolism of mangiferin by human intestinal bacteria in vitro.
METHODHuman intestinal bacteria and mangiferin were incubated under anaerobic conditions in vitro. The metabolite was separated and purified by D101 macroporous resin column and preparation high performance liquid chromatography, and its structure was identified by MS and NMR.
RESULTAfter 12 h incubation with human intestinal bacteria, the content of mangiferin metabolite reached the maximum, and it was determined as 1, 3, 6, 7-tetrahydroxyxanthen by MS and NMR.
CONCLUSIONMangiferin can be metabolized in vitro by human intestinal bacteria into its aglycone (1, 3, 6, 7-tetrahydroxyxanthen).
Bacteria ; metabolism ; Chromatography, High Pressure Liquid ; Humans ; Intestines ; microbiology ; Xanthones ; metabolism
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