Objective To verify if the multidrug resistant stains of P.aeruginosa isolated from different patients in burn ward have the same origin. Methods The susceptibility testing was performed with Etest,and the strains were typed by rep PCR with the primer ERIC2 and M13 following electrophoresis in agarose gel. Results There were multidrug resistant P.aeruginosa strains in burn ward,and analysis of the PCR productions indicated that all these multidrug resistant strains have an identical band pattern different from the sensitive strains. Conclusions The multidrug resistant strains of P. aeruginosa derive from a common origin.

Objective To investigate the influence factors work on the willingness of nursing students to participate in Mobile Learning. Methods A cross-sectional survey was conducted with 400 undergraduate nursing students. The instruments used was the influencing factors of the nursing students' willingness in Mobile Learning questionnaire. Results The resource of study about source, formation and contents, the characteristic, these scores are (12.20 ± 2.66), (14.74 ± 2.95), (11.33 ± 2.63), (11.57 ± 2.34) respectively. In addition, the score of perceived ease of use, the score of perceived usefulness and the score of study attitude, the school of preference, which are (15.02 ± 3.12), (14.81 ± 2.91), (11.43 ± 2.07), (15.18 ± 2.94) respectively. Model fit index which have these factors reached acceptable ranges. Conclusion In order to promote nursing students independent, efficient and convenient study on Mobile Learning, the designing of Mobile Learning should consider the resources characteristics and optimize techniques used.

OBJECTIVE To investigate the epidemic characteristics and drug resistance profile of clinical bacteria in hematology ward of our hospital. METHODS The susceptibility testing of clinical isolates from hematology ward was performed and the ESBLs producing strains were detected using K-B method.The results were analyzed by WHONET5. RESULTS Out of the 397 clinical isolates, 65.2% were Gram-negative bacilli and 34.8% were Gram-positive cocci.In Gram-negative bacilli,55% were Enterobacteriaceae and 44% were nonfermenting Gram-negative bacilli.In Gram-positive cocci,60.8% were Staphylococcus spp and 38.4% were Enterococcus spp.The ESBLs producing strains in Escherichia coli and Klebsiella spp were 55.8% and 19.2%,respectively.The MRSA in S.aureus and MRCNS in coagulase negative Staphylococcus were 40.9% and 95.2%,respectively.No resistance to carbapenem was detected in Enterobacteriaceae and no resistance to vancomycin was detected in Gram-positive cocci.The resistance rate of nonfermenting Gram-negative bacilli to cefoperazone/sulbactam was less than 2.1%. CONCLUSIONS The data will be useful for the early empiric administration of antimicrobial agent in hematology ward.

Adrenal gland vein sampling is an interventional method to get the blood samples from the adrenal gland vein. The blood is obtained via a catheter which is selectively inserted in the adrenal gland vein. This technique is mainly used to be diagnostic for primary hyperaldosteronism. A full knowledge of the anatomy and variations of the adrenal gland vein, serious preoperative preparation and skilled catheterization manipulation are necessary for obtaining sufficient blood sample and for reducing the occurrence of complications. Providing the physicians with definite diagnostic evidence and being technically feasible, adrenal gland vein sampling should become one of the routine examinations for clarifying the cause of primary hyperaldosteronism.

Bronchoalveolar lavage (BAL) was performed in healthy subjects (n = 12) and patients with bronchial asthma (n = ll). Between two groups there were no significant differences in total cells and percentage of alveolar macrophages (AM), lymphocytes, ncutrophils, cosinophils in BAL fluids. When AM were cultured in vitro and stimulated with dermatophagoides farinae(DPF) antigen, the quantity of PGE released by AM was significantly increased in asthmatics. When asthmatics' AM were sensitized with specific IgE positive serum and stimulated with DPF, they released more PGE, PGF2a than that when specific IgE negative serum was used (P

Treatment of Langerhans cell histiocytosis (LCH) needs to be tailored for each individual patient according to LCH classiifcation currently. Single-system LCH (SS-LCH) has an excellent prognosis. However, there is a poor prognosis in multisystem LCH (MS-LCH) with risk organs (RO) involvement and refractory or recurrent LCH (Re-LCH). The prognosis of MS-LCH with RO involvement and Re-LCH has been improved markedly accompanying with progress of chemotherapy in recent years. The 5-year survival rate of MS-LCH reached above 80%, and the effective rate of Re-LCH reached above 60% after chemotherapy. Re-LCH can be cured by hematopoietic stem cell transplantation.

Humans ; Mastoiditis ; diagnosis ; surgery ; Otitis Media ; diagnosis ; surgery

Objective To investigate the involvement of apoptosis in the lesions of patients with psoriasis. Methods The apoptosis was detected with terminal deoxynucleotidyl transferase mediated dUTP biotin nick end labeling (TUNEL), and the expression of p53, PCNA and apoptosis suppressing protein bcl 2 was assessed with immunoperoxidase technique in psoriatic lesions and normal skin. Results A large number of keratinocytes showing biochemical and morphologic features of cells undergoing apoptosis were observed in all the suprabasal layers of the psoriatic epidermis. The plaques from all patients analysed showed marked increase in the number of PCNA positive cells in the middle and basal keratinocytes, and a dramatic reduction in the number of bcl 2 positive cells in the basal cell layer. Conclusion The increased apoptosis of keratinocytes in the lesions of psoriasis might be a homeostatic mechanism to the hyperplasia of cells.

Objective To explore the clinical value of the disposable uterine cavity tissue suction tube in diagnosing abnormal u‐terine bleeding .Methods Seventy‐five patients ,who needed an endometrial biopsy because of abnormal uterine bleeding ,were se‐lected for this study .An endometrial biopsy was performed by a disposable uterine cavity tissue suction tube before the conventional suction dilatation and curettage (D&C) .The sample satisfactory rate and the diagnose accordance rate of the two methods were compared .Results The sample satisfactory rate of the disposable uterine cavity tissue suction tube and of the D&C was 85 .3%(64/75) and 94 .7% (71/75) respectively .The difference was not statistically significant(P>0 .05) .The diagnose accordance rate of the disposable uterine cavity tissue suction tube and the D&C was 90 .3% (56/62) and 93 .5% (58/62) respectively .The differ‐ence was not statistically significant(P>0 .05) .Conclusion To a certain extent ,endometrial biopsy performed by disposable uter‐ine cavity tissue suction tube can be a substitute for D&C as the initial inspection to assess abnormal uterine bleeding ,for its econo‐my ,efficiency and safety .

Objective　 To promote the therapeutic effect about the primary hypertension connected psychological factor through interventing anxiety emotion.Method　All primary hypertension patients with anxiety emotion were divided into two groups randomly.Each group was treated by lotension for 8 weeks,While group 1 combined antianxiety drugs Deanxit Result　The effective rate of two groups were 93.94%(group 1), 60.61%(group 2).Conclusion　 The therapeutic effect of group 2 is lower than that of group 1.