1.Clinical research of effect of umbilical Chinese materia medica paste on endotoxemia of cirrhosis patients
China Journal of Traditional Chinese Medicine and Pharmacy 2005;0(03):-
Objective:To investigate the efficacy and security of umbilical Chinese materia medica paste on the endotoxemia of cirrhosis patients.Methods:Based on the TAL blood cell matrix color test,80 cases of cirrhosis patients with endotoxemia positive were divided into two groups by randomly number:40 in the received regular treatment period for both groups was one month.The clinical symptoms was given to the level of treatments;Additionally,Chinese materia medica paste on umbilicus was given to the treatment group,the endotoxin,ammonia and complement component 3 in serum,cruor function,liver function,and blood routine test were statistical analysised and efficacy compared in both groups after treatment.Results:After treatment,endotoxin decreased significantly in the treatment group and the control group(P
2.Discussion on How to Exert Students' Effect as Subject in Pathology Teaching
Jiangtao NI ; Yongfen YI ; Haipeng SHI
Chinese Journal of Medical Education Research 2006;0(09):-
In the process of pathology teaching,the teacher can apply various methodologies and techniques to form student-oriented situation,to raise their interest in this discipline,to have the students actively involved in this learning process.In this way,the students become the subjects of the class so that the quality of pathology teaching can be improved.
3.Applying various methods to improve the teaching quality of oral histopathology
Jiangtao NI ; Yongfen YI ; Haipeng SHI
Chinese Journal of Medical Education Research 2005;0(06):-
Various teaching methods can be applied comprehensively to the teaching of oral histopathology. The authors make a probe into the function and significance of flexible introduction of such means as enlightened approach to teaching,metaphorical teaching method,multimedia teaching method. The paper insists that these methods will bring many benefits in that they will help to promote students’autonomous learning,stimulate their thinking,cultivate their competence for observation and analysis,broaden their vision,and consequently improve the teaching quality.
4.Cloning of mutA and expression of its coding protein
Xin SHI ; Longxing NI ; Yu TIAN
Journal of Practical Stomatology 2001;0(03):-
Objective:To obtain mutA gene of Strepto c occus mutans (Ms),and to express it in E.coli DH5?.Methods: mutA gene was amplified by PCR with specific primers from genome of Ms CH43 strain. After sequencing, the gene segment was inserted into vector pProEX and expressed in E.coli DH5?.The protein expression was induced by ITPG an d the protein products were examined by 180 ml/L SDSPAGE electrophorosis. Results:The length of PCR product was 147 bp and was identical to mu tA gene reported by GenBank.The mutA gene product was expressed in E.col i DH5? with Mr of 5.7?10 3.The maximum mutA protein product amount (20% of the total bacterial protein) was obtained when the A 600 value of DH5? was 1.666,IPTG concerntration 1.0 mmol/L and induction time 6 h.Conclus ion:mutA of Ms CH43 can be cloned and expressed in E.coli DH 5?.
5.Advance in basic and clinical research on the treatment of choradoma
Jianfa NI ; Guangxin ZHOU ; Xin SHI
Journal of Medical Postgraduates 2015;(2):203-206
Chordoma is a rare low and moderate-grade malignant tumor originated in the embryonic period residual notochord tissue.Due to its critical location, local aggressive nature, and high recurrence, chordoma is not sensitive to radiotherapy and chemo-therapy with poor prognosis .Surgery is the main treatment method .Here we present a review focusing on basic and clinical research progress of chordoma .
6.The effects of loading dose clopidogrel in preventing direct coronary stenting complication with acute myocardial infarction patients
Haiming SHI ; Xinping LUO ; Huanchun NI
Journal of Interventional Radiology 2004;0(S2):-
Objective To compare the effects of clopidogrel and ticlopidine in combination with aspirin in preventing direct coronary stenting complication in patients with acute myocardial infarction.Methods The clinical data of 74 cases with ST-segment elevation myocardial infarction were retrospectively analysed. The patients were divided into two groups. Before direct coronary stenting, clopidogrel,300mg loading dose then 75mg once daily or ticlopidine 250mg twice daily in addition to aspirin 100mg once daily were given to two groups respectively. Hemorrhage, neutropenia, thrombocytopenia were observed during 4 weeks. The frequency of clinical cardiovascular events (including cardiac mortality, myocardial re-infarction, need for revascularization) were followed up at 6 months. Results The incidence of major cardiac events in clopidogrel group was less than ticlopidine group ( 21.1% with Clopidogrel and 44.4% with Ticlopidine,P= 0.031). The incidence of light hemorrhage patents individually were 6(15.8%) vs 14(38.9%). The mean number of neutrophile white celll individually were 5.8 ?1.7 vs 4.2 ?3.6 (?10 9/L). The platelet count were 130.3 ?42.1 vs 89.2 ?53.3(?10 9/L), P
7.Application and evaluation of Sandwich teaching method in clinical teaching in the department of neurosurgery
Jinlong SHI ; Yu ZHANG ; Wei SHI ; Jian CHEN ; Lanchun NI
Chinese Journal of Medical Education Research 2016;15(10):1039-1041
Objective To explore the application effect of Sandwich teaching method in clinical teaching in the department of neurosurgery.Methods Totally 144 clinical medicine students were divided into 2 groups:Sandwich teaching group (n=72) and traditional teaching group (n=72).The analysis of test and the questionnaire of the students were conducted after the course.Comparison between the two groups was made using independent sample t test and measurement data were expressed as as ± s.P<0.05 signifies statistically significant differences.Rank sum test was used to compare the distribution of the test scores.Results The test score was statistically higher in Sandwich teaching group than control group [(76.38 ± 10.84) vs.(69.67 ± 10.50)].And the main distribution of the test scores was 70~90 in Sandwich teaching group while 60~80 in control group.Questionnaire showed that the scores of Sandwich teaching group were statistically higher than those ofthe control group in five aspects such as enhancing self-learning ability,improving the ability to analyze and solve problems,and so on.Conclusions Sandwich teaching method achieves good teaching effect and it is worth recommending in clinical teachifig.
8.Study of effect of extracting time and solvent on the quality of extract of Salvia Miltiorrhiza Bunge
Lijun NI ; Xiaohao SHI ; Xiujiao GAO ; Ning WANG ;
Chinese Traditional Patent Medicine 1992;0(10):-
Objective:To study the effect of the extracting time and solvent on the concentration of main active components in Salvia Miltiorrhiza Bunge, and to provide basic data and theoretical guide to GEP(general extraction practice). Methods: Salvianolic acid B and Tanshinone ⅡA were extracted by 90% alcohol and water respectively from Salvia Miltiorrhiza Bunge and their contents were etermined by means of HPLC at regular intervals. Results: Extracting time and solvent were significant factors in the prcess of concentrating Salvianolic acid B and Tanshinone ⅡA from extract of Salvia Miltiorrhiza Bunge. Conclusion: Water is suitable for extracting Salvianolic acids, the extracting time is 40~50 minutes; alcohol is suitable for extracting Tanshinones, the extracting time is 20~30 minutes.
9.Theoretical simulation and experimental research on constructing different compositions of ginsenoside
Lijun NI ; Junhua WANG ; Xiaohao SHI ; Ning WANG ;
Chinese Traditional Patent Medicine 1992;0(11):-
AIM: To Structure a series of diversifying combination of ginsenoside in order to provide the different samples for the pharmacological and pharmacodynamics experiments. METHODS: UNIFAC group contribution method is used for simulating the liquid liquid extraction, the proper extractants are chosen to carry out the experiments, and then the contents of the ginsenosides obtained with different extractants are detected through HPLC. RESULTS: The contents of the main ginsenoside varied with the change of the extractants' ratio, as a result a series of diversifying combination of ginsenoside are constructured. Within the ginsenoside which has greater amount, ginsenoside Rb 1 can be easily separated from Re, while Rd is hard to be separated, the experimental data were accordant to the theoretical prediction. CONCLUSION: Theoretical calculation combined with the optimizing of the extractants can structure a series of distinguished ginsenoside.
10.Issues on the automation and information of clinical laboratories
Qing WANG ; Jun WEI ; Yongmei CAI ; Qingmei SHI ; Lan NI
Chinese Journal of Medical Science Research Management 2012;25(4):269-271
This article describes the development,components and function of the laboratory automation system (LAS) and laboratory information system(LIS). And it also discusses the construction and application laboratory automation,and the operation of the automated laboratory information system.