Objective To study the protective effects of the polyphenols of vitis amurensis Rupr(PVAR) on oxidative damage in rats caused by chronic alcohol intakes.Methods Forty male Wistar rats were divided into 4 groups according rats' weights: normal control group,alcohol injury group(the rats were treated with 10 mL?kg-1 33% alcohol via i.g);PVAR group(after ingesting 200 and 400 mg?kg-1 PVAR,the male rats were treated with 10 mL?kg-1 33% alcohol via i.g).After 8 weeks,the concentrations of malondialdehyde(MDA),reactive oxygen species(ROS),glutathione(GSH) and the activities of SOD and heme oxygenase-1(HO-1) in rat liver tissues were measured.The activities of serum glutamic-pyruvic transaminase(GPT) and glutamic oxaloacetic transaminase(GOT) in rats were measured.Results Compared with alcohol injury group,the concentrations of MDA,ROS,GPT and GOT were decreased significantly(P

PURPOSE To investigate the clinical value of the T-antigen monoclonal antibody method on screening of large intestinal carcinoma. METHODS The T-antigen monoclonal antibody method and the galactose oxidase method were simultaneously used to detect T-antigen in large intestinal mucus of 207 cases. RESULTS There was not obvious difference in screening value between the T-antigen monoclonal antibody method and the galactose oxidase method, for that they had similar sensitivity (69. 2%, 67. 3%) and specificity (64. 2%, 65. 6%) in diagnosis of large intestinal carcinoma. CONCLUSION The T-antigen monoclonal antibody method was convenient, eligible and valuable on screening of large intestinal carcinoma.

Objective To analyze the practical experiences of design and its clinical application of custom-made tumor prostheses of knee. Methods A retrospective study of 41 cases of distal femur or proximal tibia tumors treated surgically from June 1999 to October 2002, was carried out. In the study, there were 29 males and 12 females aging from 11 to 69 years with an average of 35.0 years. The location of tumors were at distal femur in 23 cases and 9 cases at proximal tibia, of which 15 were osteosarcoma, 13 giant cell tumor, 3 chondrosarcoma, 5 solitary metastatic bone tumor, and 1 each of fibrosarcoma, malignant fibrohistocytoma, Ewings sarcoma, small round cell sarcoma and syndesmofibroma. The custom-made semi-constrained knee prosthesis with femoral or tibial component was implanted after extra-capsular resection of the tumor bone segment. A special femoral component with elongated construction was used in an 11-year old case for further femur lengthening. Resection of femoral artery involved by tumor and reparation by great saphenous vein was performed in one case. Results The operations of the 41 cases were all smoothly performed. 33 cases were followed up from 8 to 37 months with an average of 20.2 months. The daily life ability was recovered in 3 months after the operation. Among them, no loosening or failure of the prostheses was found, and local recurrence was found in 2 cases and distant metastasis in 3 cases. The design of knee prosthesis could provide some proper internal/external rotation and adduction/abduction movements accompanying with the flexion and extension of the knee joint without affecting the stability of the joint. For the case of proximal tibia tumor, a special design and surgical technique was developed to provide the reconstruction of patellar ligament insertion with rather good results; and the recovery of knee extension and stability were satisfied. Conclusion The custom-made semi-constrained tumor prosthesis of knee could be used as an effective method for the limb-savage management either the distal femur or proximal tibia tumor cases.

Objective To evaluate the efficacy of Shikani laryngoscope-guided nasotracheal intubation in patients undergoing maxillofacial surgery. Methods One hundred ASA Ⅰ or Ⅱ patients with body mass index ＜25 kg/m2 , aged 18-64 yr, undergoing maxillofacial surgery under general anesthesia requiring nasotracheal intubation were included in this study. Fifty patients without anticipated difficult airway in preoperative assessment were randomly divided into 2 groups ( n = 25 each): Macintosh luyngoscope group ( group M ) and Shikani Optical Stylet (SOS) group (group S1 ). The other 50 patients with mouth opening ＜ 3 cm but without difficult ventilation by mask were randomly divided into 2 groups ( n = 25 each): fiberoptic bronchoscope group (group F) and SOS group (group S2 ). Tracheal intubation was performed under the guidance of laryngoscopes after induction of anesthesia, and then the patients were mechanically ventilated. The degree of glottis exposure and epistaxis was evaluated. The rate of successful intubation, rate of successful intubation at first attempt, intubation time and complications were recorded. Results Compared with group M, the rate of successful intubation, rate of successful intubation at first attempt, and degree of glottis exposure were significantly increased, the intubation time was significantly shortened, and the degree of epistaxis was significantly decreased ( P ＜ 0.05), but no significant change was found in the complications in group S1 ( P ＞ 0.05). Compared with group F, the intubation time was significantly shortened ( P ＜ 0.05), but no significant change was found in the rate of successful intubation, rate of successful intubation at first attempt and complications in group S2 ( P ＞ 0.05). Conclusion Shikani laryngoscope can expose glottis better and the rate of successful intubation at first attempt is higher, thus it is more suitable for nasotracheal intubation in patients undergoing maxillofacial surgery.

Objective To design a slide support nursing bed for the patient after cross leg flap transplant operation.Methods The bed was designed based on the principle of four-link-type mechanism slide,which was composed of a bed body,slide support,sliding rails,locking bolt,connector bar for sliding rails,extension arm,leg support and etc.Accurate localization of leg support was executed by zero setting,readjustment and etc.Results The bed could be adjusted up and down,back and forth or at different angles so as the compression on the cross leg flap was avoided to enhance the comfort of the patient after cross leg flap transplant operation.Conclusion The bed gains advantages in convenience,safety,effect and etc,and is worthy promoting clinically for the patient after cross leg flap transplant operation.

0.05).Conclusion The local relapse rate and the survival rate within 3 years has no significant difference between patients with lower rectal cancer of Duck stage A after sphincter-preserving surgery and Miles surgery.

Immunohistochemical localization of many sorts of tumor tissues from paraffin sections was studied using the ABC method with the monoclonal antibody secreted by the established two anti-osteosarcoma cell lines. It showed that the OS-McAb1 and OS-McAb2 had a negative reaction on the tested benign tumors and malignant tumors of epithelial tissue. Of the tested malignant tumors of mesenchyma, the antibodies had a positive reaction on some osteogenic sarcoma. They did not cross-react with various normal adult or fetal tissues, indicating that the OS-McAbs had a rather high specificity. They had a certain practical value in the immunopathologic diagnosis of malignant osteogenic tumors.

Objective:To investigate the effects of N-acetylcysteine(NAC)on the number of Clara cells and secretion of Clara cell16000(CC16)protein in murine asthmatic model.Methods:The murine asthmatic model was established by sensitiz-ing and challenging BALB/c mice with ovalbumin(OVA).Thirty mice were divided into control,asthmatic and NAC groups (n=10). The number of Clara cells and synthesis of CC16were determined by immunohistochemistry.The CC16level in bronchoalveolar lavage fluid(BALF)was determined by Western blot.Results:The proportions of Clara cells in terminal and respiratory bronchioles were(58.05?3.75)%and(63.70?1.79)%in the asthmatic group,(74.54?5.81)%and (78.46?1.68)% in the control(P

Objective To evaluate the feasibility,safety,post-operative short and long-term outcomes of laparoscopic-assisted D2 radical distal gastrectomy in treating advanced gastric carcinoma without serosal invasion.Method From May 2007 to May 2013,54 cases of advanced gastric antral cancer without serosal invasion underwent laparoscopic-assisted D2 radical distal gastrectomy (laparoscopic group),54 demographically and pathologically-matched cases undergoing open surgery served as control.The surgical risk,post-operative recovery and follow-up results were compared.Result Surgery was successful in all patients.The mean operation time in laparoscopic group and open group was (168 ± 31) mins and (157 ±20) min respectively,the difference was significant (t =2.237,P =0.027) ; Intra-operative blood loss was (151 ± 56) ml and (213 ± 86) ml (t =4.45,P =0) ; Post-operative intestinal function recovery time was (2.7 ± 0.7) d and (3.4 ± 0.5) d (t =5.4,P =0) ; Lymph node dissection number was (26.4 ± 4.2) and (24.8 ±5.2) (t =1.769,P=0.08).Post-operative complication rate was 4％ and 11％ (P =0.142).There was no perioperative mortality in either group.Post-operative pathological stage of Ⅰ B,Ⅱ A,Ⅱ B,ⅢA was 8,17,24,5 cases in laparoscopic group and 9,14,23,8 cases in open surgery group.108 cases were followed up from 7 to 79 months.In laparoscopic group,8 cases had local recurrence or distal mestastasis,and 4 cases died from tumor; In open group,9 cases had local recurrence or distal mestastasis,and 6 cases died from tumor.Conclusions Laparoscopic-assisted D2 radical distal gastrectomy in treating advanced gastric cancer without serosal invasion is feasible,safe and advantageous in minimal invasion and rapid recovery,with good short and long-term outcomes.

Objective:To investigate the structure of alveolar bone around incisors in patients with verical facial type of skeletal ClassⅡ by CBCT.Methods:60 skeletal Class Ⅱ patients were divided into low angle,average angle and high angle cases(n =20)by FMA.CBCT scanning was made and the thickness of the labial and lingual alveolar bone around the incisors in each subject was meas-ured.The measurements were analyzed using ANOVA.Results:The total width of alveolar bone at the root apical level of maxillary and mandibular incisors,the lingual thickness of alveolar bone at the root midpoint of maxillary incisors and labial thickness of alveolar bone at the root midpoint of mandibular incisors in the high angle cases were thinner than that in the average and low angle cases(P <0.05),but there was no significant difference in labial thickness of alveolar bone at the crest among 3 groups.Conclusion:The alveo-lar bone thickness around incisors of high angle patients is thinner,more attention should be paid for the alveolar bone absorpation and tooth root exposure in these patients.