Intestinal trefoil factor(ITF)is a low molecular weight polypeptide expressed in intestine. ITF is also found in the brain,respiratory,eyes and so on.To study recombinant ITF will have an important impact for the treatment of gastrointestinal disease, respiratory tract disease, eyes disease and so on.

Objective To investigate the changes and clinical significance of hemorrheology and platelet parameters examination in patients with hypertension. Methods Hemorrheology and platelet parameters were detected in 78 patients with hypertension and in 72 healthy subjects as control group. Then difference were compared. Results The hemorrheology and platelet parameters in he patients with hypertension at stage Ⅰ were no different in comparison with the control group(all P＞0.05) ,which at stage Ⅱ and Ⅲ were significantly different in comparison with stage Ⅰ and the control group ( all P ＜ 0. 05). and at stage Ⅲ were significantly different in comparison with stage Ⅱ(all P＜0.05). Conclusion The changes ofhemorheology and platelet parameters were closely related with hypertension,which were helpful to the clinical diagnosis of hypertension and had important significance to the therapy, monitor and prognosis of hypertension.

Postoperative pancreatic fistula (POPF) is the most common complication after pancreaticoduodenectomy (PD) and remains a challenge to pancreatic surgeons.In pancreaticojejunostomy,the methods of reconstruction,experience and operational techniques of surgeons are closely related to POPF.Based on a literature review and on personal experience,the author presented the basic requirements and principle in reconstructing a safe pancreaticojejunostomy (PJ).The traditional methods of pancreaticojejunostomy and their recent developments were evaluated,with their advantages and disadvantages compared.The indications of various types of PJ and the special skills required were summarized.According to his own experience,the author described in detail his recommended method of anastomosis.

Objective To explore the ways to strengthen the mode and effect of the standard community management of the diabetes,and the management model of diabetic mellitus that adopt to current situation of China.Methods 80 diabetics patients were divided into intensive group and common group,and the period of the treatment is three consecutive months.We observed the levels of FBG,2hPG,HbA1c and the cost of drugs.Results The levels of FBG(6.82 ± 1.23),2hPG[(9.51 ± 2.18)mmol/L]and HbA1c(7.52 ± 1.31)％ in intensive group were significantly lower than common group[(8.65 ± 2.31)mmol/L、(12.22 ± 3.11)mtmol/L,(10.32 ± 3.51)％](t =3.12,3.25,3.56,3.69,all P ＜ 0.05).The cost of drugs in intensive group[(621.5 ± 204.5)yuan]was significantly lower than common group[(796.5 ± 240.6)yuan](t =3.69,P ＜ 0.05).Conclusion The ways to strengthen the mode could spend less but control disease well,and improving the existing community diabetes management should be truly effective in reducing medical costs.

Objective To investigate the feasibility,technique and clinical significance of preserving the great auricular nerve in parotid benign tumors surgery during the first postoperative year.Methods Fifty-two patients with parotid benign tumors were randomly divded into 2 groups.24 patients (group sacrificed) underwent classic parotidectomy with sacrifice of the great auricular nerve.The surgeons spare the nerve in the 32 patients with FOCUS ultrasonic harmonic scalpel (group preserved).Results After surgery,both groups showed lower levels of subjective sensation and sensory function test at auricle.These alterations were less pronounced in group preserved.Both groups showed improvement over time.In group preserved the sensory function reached normal level by 12 months after surgery.The recuperation in group sacrificed was partial at 12 months after operation.Conclusion The great auricular nerve preservation during parotidectomy is feasible,It can decrease sensory disturbance in the early postoperative period and avoid the permanent sequelae that occur when the nerve is sacrificed.The FOCUS ultrasonic harmonic scalpel technique can reduce the operation time.

Twenty-three joints in 14 cases of chronic synovitis were treated with intra-articular injection of 32 p-chromic phosphate, including 19 knee joints, one elbow joint with rheumatoid arthritis and 3 knee joints with degenerative arthritis that was recractory to medical treatment. Results of 23 joints evaluated at end of one year were excellent in 43.5$, good in 30.5%, .fair in 13% and poor in 13%. There was a good correlation between the radiographic stage of the joints and the response to treatment. Patients in stage 1 and 2 were more likely to have good to excellent response. Cur study shows that radiation synovectomy is a simple, safe and effective treatment than other managements for chronic synovitis

In order to provide highly purified preparation of human ieukoregulin (LR) for the exploration of its anticancer mschatiism, crude preparations of LR and lymphotoxin(LT) derived from human spleen cell cultures induced with phytohem-agglutinin and diterp;ne ester l2-O-tetradecanoylphorbol-i3-acetate were purified by using four purification nmhois : (a) Con A-Sepharose 48 affinity chromatography, by which crude human LR or LT was purified to maximum specific activity of 85 333 U/mg protein or 13333 U/mg protein with a recovery of 28.1% or 31.9%, respectively 5 (b) DEAE-Sjphadex A-50 ion-exchange chromatography, by which partially purified LR or LT obtained after Con A-Sepharose 4B affinity chromatography was further purified 2.44-fold or 3.8o-fold with a recovery of 96% or 92%, respectively ; (c) DEAE-22-celluiose ion-exchange chromatography, by which crude human LR or LT was purified to maximum specific activity of 11 294 U/mg protein or 13 176 U/mg protein with a recovery of 87.5% or 89.6%.respectively ; (d) Blue-Sepharose CL-6B affinity chromatography, by which crude human LR or LT was purified to maximum specific activity of 51.89 U/mg protein or 2594 U/mg protein with a recovery of 53.75% or 5t3.G7%, respectively.Our data indicate that Con A-Sepharose 4B affinity chromatography, DEAE-Sephadex A-50 ion-exchange chromatography.DEAE-22-cellulose ion-exchange chromatography and Blue-Sepharose CL-6B affinity chromatography may be useful in the purification of LR or LT.

The quantitative determination of octanoate content in albumin product was performed by acid base titration directly after the water phase was mixed up with the ether phase using the mixture solvent solution. The three factor optimum seeking principle was used to seek the optimum concentrations and volumes of components in mixture solvent solution and the optimum conditions of extraction. The method had its recovery rate of 99. 8+0. 89%(n=6) and variation coeff-cient (CV)of 0. 89%(n= 16),indicating that the method was a precise and reliable one.

OBJECTIVE: To study the effects of Semen Litchi on the expressions of S180 and EAC tumor cells and Bax and Bcl-2 proteins in rats. METHODS: EAC, S180 and hepatic carcinoma tumor-bearing mice models were established in mice by inoculating them with tumor strains. EAC, S180 and hepatic carcinoma suspensions were diluted respectively with normal saline in a ratio of 1∶1 for the preparation of tumor-bearing ascites suspension. Each rat was subcutaneously inoculated with the mixture 0.2 mL via axilla 24 hours before medication, with the whole sterile operation finished within 60 minutes. After treated with Semen Litchi for 10 consecutive days, the growth of EAC, S180 and hepatic carcinoma in mice was observed. RESULTS: The growth of EAC and S180 and hepatic carcinoma cells in mice was significantly inhibited by Semen Litchi. CONCLUSION: Semen Litchi can inhibit the growth of S180 and EAC tumor cells in vitro and in vivo, and its antitumor effect is related to its ability to promote apoptotic of tumor cells.