At the Hospital No 103, PCR technique was used for detecting M. Tuberculosis immediately in pleural fluid of 39 patients with tuberculosis pleuresia and 10 patients with primary cancer pleuresia. By PCR reaction tuberculosis bacterial DNA was detected successfully in pleural fluid of 92,3% of paitents. The technique had got 63,8% of sensitivity and 100% of speccificity. By immunochromatography, IgG antibacterial antibody in pleural fluid was detected in 89,7% of patients. The combination of these 2 methods were necessary in giving high efficacy in definite diagnosis. The technique of PCR could be applied in various institution for earlier diagnosis with higher accuracy.
Polymerase Chain Reaction ; Diagnosis ; Tuberculosis

This study aims to estimate the costs of various unintentional injuries in Ba Vi district during one year, and to describe how the economic burden of unintentional injuries was distributed between households, government and the health insurance agency. Our cohort study involved four cross sectional household surveys among sampled communities in Ba Vi district during the year 2000, each asking about injuries in the preceding 3 months. The costing system in public health care in Viet Nam was applied as well as information from the victims. The total cost of injuries for the Ba Vi district was estimated to be 3.412.539.000 VND. Equivalent to the yearly income of 1800 people. 90% of this economic burden fell on households, only 8% on government and 2% on the health insurance agency. The cost of a severe injury to the victim corresponded to approximately 7 months of earned income. Home and traffic injuries together accounted for more than 80% of the total cost, 45% and 38% respectively.
Cost of Illness ; Wounds and Injuries ;

Research on 31 patients (include 24 man and 7 woman) at 20 to 60 years old who treated active hepatitis B at The army centre hospital 105 from 1/1999 to 6/2002. Result: HBV-DNA level in plasma increase follow agreeable rate with SGPT content: SGPT increase to > 100U/L correlative HBV-DNA patients(+) ≥10copies, 16,13% HBV-DNA patients (-) from 5 to 10 copies. The development of the disease in active hepatitis B patients was showed at relationship of marks of HBV with HBV -DNA(+) 70,96%, Anti-HBV-IgG (+) 100%, anti-HBc0IIgG (+) and anti-HBc-IgM (+) 12,90%, HBV-DNA (+) and HBeAg (+) 41,94%, HBV-DNA(+) and anti-HBe (-) 38,72%. HBV-DNA may be importance mark in following up development of the disease
Hepatitis B ; Liver ; Disease

Background: The rate of Khmer ethnic minority living in An Giang, Kien Giang and Hau Giang were 3%, 12.9% and 3%, respectively.These provinces had some specific factors such as low education, income and knowledge about HIV/AIDS, high risk behavior and it is difficult to practice intervention methods therefore the awareness of HIV infection risk on this group is required. Objectives: To determine the HIV prevalence, describe knowledge, attitude and risky behaviors that led to syphilis and HIV infection of the Khmer ethnic group, which are used to propose suitable intervention and prevention models. Subject and methods: A cross sectional survey was conducted from September 2006 to June 2007 in the Khmer ethnic group, including both genders at the age ranging from 15 to 49, living in some districts of An Giang, Hau Giang and Kien Giang. Results: A total of 877 households and 2.400 people were interviewed. 98.62% of the people agreed to take a blood test for HIV. The average age of first intercourse was 20. The condom usage proportion was very low. The number of men using condoms in the latest intercourse with their wives or lovers accounted for only 7.2%. Moreover, only 3% of the women used condoms in the last 12 months. The drug usage was 0.6% in Hau Giang and 0.1 % in Kien Giang. The knowledge about HIV/AIDS of both men and women were 9.2% and 4.5%. The prevalence of people, who made HIV test in the three provinces accounted for only 3 to 4.6%. HIV infection rate was 0.2% in An Giang, 0.5% in Kien Giang and 0.5% in Hau Giang. Syphilis infection rates were 1.6%, 1.4% and 1.5%, respectively. Conclusions: Appropriate communicative model is an effective way for the prevention HIV/AIDS among Khmer ethnic group.
HIV/AIDS ; Khmer ethnic minority

Introduction: In Vietnam, the andrology field has been developed in recent years. Some studies have indicated the reasons for male infertility. Amongst them, the most common reason is abnormal semen. Asthenozoospermia rate was found at a higher ratio in abnormal semen analysis. \r\n', u'Objectives: To identify the characteristics of sperm and concentration of FSH, LH and Testosterone in serum; and the association between the sperm morphology and sperm motility as well as the concentration of FSH, LH and Testosterone in serum of asthenozoospermia men. \r\n', u'Subjects and methods: 90 asthenozoospermia male partners of infertile couples. The semen was analyzed at the Laboratory for Tissue and Embryo Preservation, Hanoi Medical University. The hormone concentration was measured by the Automated Chemiluminescence System ACS 180. \r\n', u'Results: The mean semen volume: 3.0 \xb1 1.1 (ml); sperm density: 104.7 \xb1 86.7 million/ml; rapid motility rate: 5.9 \xb1 6.4 (%); sperm vitality rate: 67.4 \xb1 20.1 (%); normal morphology rate: 14.4 \xb1 6.9 (%). In serum, the concentration of FSH was 5.3 \xb1 2.5 lUlL; LH: 4.1 \xb1 1.8 IU/L. Testosterone: 18.3 \xb1 9.4 nmol/L. Conclusions: Among the studied asthenozoospermia men, normal semen volume was found at 91.11 %; normal sperm vitality parameter: 84.4%; normal morphology parameter: 45.56%; normal level of FSH concentration: 47.7%; normal level of LH concentration: 26.66%; normal level of Testosterone concentration: 78.89%. There was a strong association between the sperm morphology and sperm motility as well as serum Testosterone concentration. \r\n', u'
Semen analysis ; Asthenozoospermia ; FSH ; LH ; Testosteron

Background: Laparoscopic cholecystectomy is the first laparoscopic surgery that was performed at Cho Ray hospital in September 1992. In 2005, Quang Tri general hospital has performed successfully the first case of laparoscopic appendectomy. Objectives: To access the preliminary results of application on endoscopic surgery in Quang Tri general hospital. Subjects and method: A prospective study was conducted on 140 patients with laparoscopic surgery or retroperitoneal surgery, was performed in Quang Tri general hospital from September, 2005 to April, 2006. Results:Among 140 patients was operated, there was only one case of postoperative intestinal obstructive complication (accounted for 0.7%), no case of death. Operative aged was between 11 and 70 years old. The average surgical time was 65 minutes for cholecystectomy, 35 minutes for appendectomy, 85 minutes for ureterolithotomy, 80 minutes for gynecological diseases. 2 cases of cholecystectomy and appendectomy changed open surgery. Conclusion: Laparoscopic surgery is a safe and effective method. It can be developed in provincial hospitals. Quang Tri general hospital has successful preliminary developed the new technique.
Laparoscopy ;

Background: In Vietnam, Dengue Fever/Dengue Haemorrhagic Fever (DF/DHF) is one of ten contagious diseases causing epidemics with the highest prevalence.\r\n', u'Objectives: To describe some characteristics of DF/DHF epidemic in Khanhhoa province in 2005. \r\n', u'Subjects and methods: The descriptive study based on available data obtained from the surveillance reporting system.\r\n', u'Results: Total reported cases were: 5.365 (morbidity rate = 469.58 per 100.000) and 4 deaths (CFR = 0,074%).446 of 1.661 Mac Elisa tested cases were positives (positively = 26.9%). The positive cases in male were higher than that in female. Almost all of the positive cases were less than 15 years of age. Virus serotype D1 and D2 were isolated in the epidemic. 5 of 7 districts (except 2 mountainous districts) had outbreaks. The epidemic started in April and stopped in December. Ninhhoa district was the first location of the outbreak and Vanninh district was the last one. Geographical distribution of the epidemic was as follows: costal communes=54.6%, delta communes = 30.3%, urban communes =15.1%. The epidemic level was medium (>20% - <50% of communes having the epidemic). "Pilot intervention communes" had 4 times the higher risk of DF/DHF than "non pilot intervention communes". \r\n', u'Conclusions: IEC activities for preventing DF/DHF should be strengthened. For maintaining the low mosquito and breutau index, we need routine preventive actions. Authorities need to consider and to consolidate the activities of Pilot intervention communes. Coastal communes should be supplied with running water. \r\n', u'\r\n', u'
dengue fever/dengue haemorrhagic fever ; epidemic

As part of our continued study on the chemical constituents of Willughbeia edulis stems, a new dimeric lignan named edulignan (1) was isolated from its EtOAc-soluble extract. Based on NMR spectroscopic interpretation, the planar structure of 1 has been suggested to have two 2-substituted 4-chromanone subunits with different stereochemical configurations. In addition, the MS/MS analysis of the products obtained by acidcatalyzed hydrolysis of 1 was supportive of its structure. Unfornatually, the new compound 1 did not show α-glucosidase inhibitory activity with an IC 50 value > 250 μM.

BACKGROUND: Currently, it is recognized that water polluted with toxic heavy metal ions may cause serious effects on human health. Therefore, the development of new materials for effective removal of heavy metal ions from water is still a widely important area. Melanin is being considered as a potential material for removal of heavy metal from water. METHODS: In this study, we synthesized two melanin-embedded beads from two different melanin powder sources and named IMB (Isolated Melanin Bead originated from squid ink sac) and CMB (Commercial Melanin Bead originated from sesame seeds). These beads were of globular shape and 2-3 mm in diameter. We investigated and compared the sorption abilities of these two bead materials toward hexavalent-chromium (Cr) in water. The isotherm sorption curves were established using Langmuir and Freundlich models in the optimized conditions of pH, sorption time, solid/liquid ratio, and initial concentration of Cr. The FITR analysis was also carried out to show the differences in surface properties of these two beads. RESULTS: The optimized conditions for isotherm sorption of Cr on IMB/CMB were set at pH values of 2/2, sorption times of 90/300 min, and solid-liquid ratios of 10/20 mg/mL. The maximum sorption capacities calculated based on the Langmuir model were 19.60 and 6.24 for IMB and CMB, respectively. However, the adsorption kinetic of Cr on the beads fitted the Freundlich model with R values of 0.992 for IMB and 0.989 for CMB. The deduced Freundlich constant, 1/n, in the range of 0.2-0.8 indicated that these beads are good adsorption materials. In addition, structure analysis data revealed great differences in physical and chemical properties between IMB and CMB. Interestingly, FTIR analysis results showed strong signals of -OH (3295.35 cm) and -C=O (1608.63 cm) groups harboring on the IMB but not CMB. Moreover, loading of Cr on the IMB caused a shift of broad peaks from 3295.35 cm and 1608.63 cm to 3354.21 cm and 1597.06 cm, respectively, due to -OH and -C=O stretching. CONCLUSIONS Taken together, our study suggests that IMB has great potential as a bead material for the elimination of Cr from aqueous solutions and may be highly useful for water treatment applications.
Adsorption ; Chromium ; chemistry ; Kinetics ; Melanins ; chemistry ; Waste Disposal, Fluid ; methods ; Water Pollutants, Chemical ; chemistry ; Water Pollution, Chemical ; prevention & control ; Water Purification ; methods

Objectives: The prevalence of posttraumatic stress disorder (PTSD) has increased, particularly among individuals who have recovered from coronavirus disease 2019 (COVID-19) infection. Health literacy is considered a “social vaccine” that helps people respond effectively to the pandemic. We aimed to investigate the association between long COVID-19 and PTSD, and to examine the modifying role of health literacy in this association. Methods: A cross-sectional study was conducted at 18 hospitals and health centers in Vietnamfrom December 2021 to October 2022. We recruited 4,463 individuals who had recovered from COVID-19 infection for at least 4 weeks. Participants provided information about their sociodemographics, clinical parameters, health-related behaviors, health literacy (usingthe 12-item short-form health literacy scale), long COVID-19 symptoms and PTSD (Impact Event Scale-Revised score of 33 or higher). Logistic regression models were used to examine associations and interactions. Results: Out of the study sample, 55.9% had long COVID-19 symptoms, and 49.6% had PTSD.Individuals with long COVID-19 symptoms had a higher likelihood of PTSD (odds ratio [OR], 1.86; 95% confidence interval [CI], 1.63–2.12; p < 0.001). Higher health literacy was associated with a lower likelihood of PTSD (OR, 0.98; 95% CI, 0.97–0.99; p = 0.001). Compared to those without long COVID-19 symptoms and the lowest health literacy score, those with long COVID-19 symptoms and a 1-point health literacy increment had a 3% lower likelihood of PTSD (OR, 0.97; 95% CI, 0.96–0.99; p = 0.001). Conclusion Health literacy was found to be a protective factor against PTSD and modified the negative impact of long COVID-19 symptoms on PTSD.