Fasciola eggs were collected from stool of a woman aged 21 years old in Nghe An province. Miracidium were obtained from these eggs after 20 days culture in the laboratory. A portion of the mitochondrial-encoded nad1 gene from these miracidiums were amplified using polymerase chain reaction (PCR) and comparatively aligned with the known corresponding sequences of Fasciola gigantica taken from cattle in Hoa Binh, Lai Chau, Lang Son, Ninh Binh and international strains (from Korea, Japan and Indonesia). The results showed high similar co-efficient of nucleotide 98-99% and amino acid 95-99%, but lower rate than that of Fasciola hepatica (Australian and American) (90-92%). However, it is suggested that the Vietnamese F.gigantica have had the genetic signs hybridized with F.hepatica
Women ; diagnosis ; Ovum ; Fasciola ; Molecular Diagnostic Techniques

Twenty persons in the Quai To commune, Tuan Giao district of the Northwest province of Dien Bien were infected with Trichinella on September 2004. All these 20 patients were determined to have eaten raw lean pork ("lap" dish) from a local pick. Typical clinical symptoms were found in all the patients. Most patients were male (85%) and aged from 22 to 65 with most of 31-40 years old. The clinical symptoms appeared within 1-2 weeks in all patients and most of them had fever (100%), muscular pain (100%), difficult moving (100%), oedema (95%), dysphagia (90%), weight loss (90%), itching (85%), dyspnea (80%), diarrhea (50%), lisp (40%), abdominal pain (35%)
Trichinella ; epidemiology ; diagnosis

Ten fresh water fish species (Hypothalmichthys molitrix, Tilapia mossambica, Mylopharyngodon piceus, Cirrhina molitorella, Squaliobabus curriculus, Cyprinus carpio, Carassius carassius, Ophicephatus, Pluta alba, Clarias fuscus) collected from five markets in Ha Noi city: Dang Tran Con (Dong Da district), North Thanh Xuan (Thanh Xuan district), Thanh Cong (Ba Dinh district), Van Dien (Thanh Tri district), and Phung Khoang (Tu Liem district) were examined for Clonorchis metacercaria and Gnathostoma larvea. Seven species were found to be infected with Clonorchis metarcecaria and only eels (Fluta alba) infected with Gnathostoma larvae. The samples of Clonorchis sp metacercaria were identified as Clonorchis sinensis and Gnathostoma larvae as Gnathostoma spinigerum
Clonorchis sinensis ; Larva ; Gnathostoma ; fishes ; Fresh Water

The survey was carried out between June - December 2002 and May - October 2003 in the different sites of Ha Noi. 138.298 pigs and 9.755 cattle were inspected. One of the 138.298 inspected pigs was found to be infected with Teania solium larva (0.000723%). No one of 9.755 inspected cattle was found to be infected. The survey was also carried out in the pork shops in five districts of Ha Noi city: Dang Tran Con (Dong Da district), North Thanh Xuan (Thanh Xuan district), Thanh Cong (Ba Dinh district), Van Dien (Thanh Tri district) and Phung Khoang (Tu Liem district). In 5.570 times of inspection in the pork shops, only one sample (0.018%) was found to be infected with Teania solium larva. This infected case was found from 1.305 samples (0.08%) collected in the Thanh Cong market. None of 4.265 inspected samples was positive with Teania solium. Also none of the samples collected from meat of cows and buffalo sold in 1.557 shops was found positive
Taenia solium ; Swine ; Meat ; epidemiology

There are various genera of taeniasis but in Vietnam Taenia saginata, Taenia solium and Taenia asiatica. Among them, only Taenia solium is currently identified as the cause of cysticercosis. Taeniasis and certicercosis are distributed largely. The incidence of taeniasis is 0.5-12.6%. Certicercosis is occured at least in 49 provinces and cities in whole of the country, with the incidence of 5.7% in Bac Ninh and 19.7% in Ho Chi Minh city. These conditions affect considerably on community health. In local health services, the treatment of cysticercosis is now difficult. At basic level the collaboration of health services and veterinary health is recommended in the control of tamiasis and certicercosis
Taeniasis ; Cysticercosis ; Humans ; epidemiology

A 21 -year -old woman patient, from middle of February 2003, she had got fatigue, weight loss 5-6 kg within 3 weeks, bad appetite, indigestion. Then she had pain in right lower rib, 390C fever, cough, dry vomitting. She had got diagnosis of hepatic abscess and unsuccessfully treated by 10 days antibiotics. She had got cecal abscess and abscess block was removed. Stool examination found Fasciola hepatica eggs and ELISA serum test was (+) with F.gigantica antigen (collected from buffaloes and cows) by title of 1/12800, acidophil white blood cell 12%. Triclabendazole 10mg/kg gave good results.
Fasciola ; Vietnam ; Eggs ; Genome, Mitochondrial

Analysis samples of meat and seafood... in Hanoi markets to determine infections of larva of parasites. Results: among 144,390 beef samples and 2,237,000 pork samples, prevalences of parasite infection were 0.027% and 0.003%, respectively. The rate of liver fluke metacercaria in fresh water fish was 1.7-21.7% (mean rate 5.2%), rate of Gnathostoma infection in snakehead fish and ell was 4.8-11.4%. Furthermore, in endemic areas of lung fluke, infection rate of Paragonimus metacercaria in fresh water crabs and fish was 9.7%-98.1% (mean rate 63%).
Food Contamination ; Parasites ; Food ; Epidemiology

A survey concerning the infestation of clonorchis sinensis in human was conducted in 4 communes of Ninh Binh and Nam Dinh provinces, from each, 100 households with their members of above 6 years old of age were enrolled randomly. Results showed a prevalence of 31.3 – 37.3% in Nam Dinh and 22.5% in Ninh Binh epidemic areas. The prevalence in male subjects was higher than in female (45.5 –47.6% versus 10.9 – 26.1%). The age group of 40-49 years old had had the highest prevalence (51.9%). There, 35.9 – 80.4% of people had consumed raw fish.
Opisthorchis ; Epidemiology ; Clonorchis sinensis

Angiostrongylosis is roundworm disease caused by Angiostrongylus. Angiostrongylus including 20 species parasitize mainly in mouse, among which two species cause disease in human are Angiostrongylus cantonensis and Angiostrongylus costaricensis. Angiostrongylus cantonensis was found by Chen H.T in 1935 in China. For pediatric case records that having meningitis syndrome with symptoms of fever, headache, paralytic, cerebrospinal fluid clear or unclear but eosinophil increasing highly, it needs to think of meningitis caused by parasites and do immune test to Angiostrongylus to identify the cause. Early treatment will have good results and avoid severe consequences
Meningoencephalitis ; Angiostrongylus

b cytochrome oxydase gene fragment of the mitochondric geneme includes 652 nucleotides and aminoacide of taenia solium isolated from Vietnamese [symbolized as TsoN (VN)] was received by PCR reaction, purified, cloning and expressed in sequence. Results were compared with those of corresponding sequence of some genera of Taenia solium in China (T. saginata) in Taiwan (Taeniaasiatica) and in Viet Nam. Cysticercoid larve of Taenia solium isolated from Vietnamese was similar with that isolated from pig in Viet Nam and China, but there are high difference between the genera T. saginata (in China) and T. asiatica (in Taiwan and Viet Nam). The analysis determinated various varieties of eysticercoid larve as Taenia sodium’s larva. This is the first determination of this genus in Viet Nam
Taenia solium ; Cysticercus ; Patients