Fasciolopsis buski is common parasite in pigs. Some patients who infected by adult worms of Fasciolopsis buski in several areas, including the provinces of Ninh Binh and Nghe An were sampled. Genome of 18S ribosomal RNA with 1950 nucleotides from giant intestinal fluke (Fasciolopsis buski) was obtained using polymerase chain reaction (PCR) and sequencing approach for comparative analysis each others and with the data deposited in GenBank (L06668). The adult worms from Ninh Binh and Nghe An were morphologically identified as Fasciolopsis buski. The genetic analysis revealed that the 18S rRNA gene of the giant intestinal flukes isolated in Ninh Binh and Nghe An were similar (homogenous 100% of nucleotides) and showed nearly absolute identity with only 2 divergent nucleotides of 0.01% (2/1950 nucleotides) to the 18S rRNA of the pig Fasciolopsis buski as the data deposited in GenBank (L066608). However, 18S ribosomal RNA of the adult worms of human Fasciolopsis buski collected from the provinces of Ninh Binh and Nghe An were similar to one another
Fasciolidae ; RNA

Triclabendazole was used in treatment of 249 fascioliasis patients selected from 25 provinces including 19 Northern and 6 Southern ones. Two different doses were used for two groups: 10mg/kg/body for 226 patients, and 20mg/kg/body for 43 others, twice per day with the interval 6-8 hours from meals. Symptoms on these fascioliasis patients were found as positive ELISA test with Fasciola gigantica antigen (100%), prejudice in liver by ultrasound (87.9%), pain of liver (87.1 %), eosinophilia (63.5%), plodding (26.1 %), fever (39.8%), digestive disorder (20.1%) and positive stool examination with Fasciola egg (16.9%). Most of symptoms were decreased and disappeared within 1 month after treatment except for pain of liver in some patients that lasted longer and disappeared within 6 to 12 months after treatment. The cure rate was 92.9% for 1 month after treatment, 95.2% for 3 months after treatment and 100% for 6 months after treatment. Ultrasound prejudice in liver decreased and disappeared 80.9% for 1 month, 92.6% for 3 months, 96.3% for 6 months and 100% for 12 months after treatment. Eosinophilia rate returned to normal of 90.7% for 6 months and 100% for 12 months after treatment. ELISA test with F. gigantica antigen become negative of 89.4% for 6 months and 100% for 12 months. GOT, GPT, urea and creatinin tests had not pathological change by 1 month using triclabendazole. Side effect of triclabendazole was inconsiderable and disappeared without medical treatment. Triclabendazole may be recommended in treatment for fascioliasis in Vietnam with doses of 10 or 20 mg/kg of body weight.
Fasciolidae ; Therapeutics ; Benzimidazoles

A region of mitochondrial cytochrome oxidase 1 gene (coxl) for Clonorchis sp samples from the provinces of Thanh Hoa and Nam Dinh, and Opisthorchis sp from human in Phu Yen province, Vietnam was analyzed by PCR. Nucleotide sequence of these coxl fragments was used to compare with Opisthorchis viverrini, strain Khon Ken (Thailand) and Clonorchis sinensis sequences originated from China and Korea. The results revealed that nucleotide sequence of Opisthorchis sp from Vietnam had absolute homology to O. viverrini (Thailand) but different from C. sinensis (from China and Korea). Clonorchis sp from Vietnam was similar with Clonorchis sinensis from China and Korea. Opisthorchis sp isolated in Phu Yen is, thus, molecularly indentified as Opsithorchis viverrini and Clonochis sp in Thanh Hoa and Nam Dinh is Clonorchis sinensis. Two species are different in morphology and molecular level.
Mitochondria ; Clonorchis sinensis

Since 2002, adult worms of human small liver fluke in some provinces were identified by morphology and confirmed by molecular method as Clonorchis sinensis in Thanh Hoa, Ninh Binh, Nam Dinh and Bac Giang province; Opisthorchis viverrini in Phu Yen and Binh Dinh province. This study, using polymerase chain reaction (PCR), a portion of the cytochrome oxidase 1 (410 nucleotide and 136 amino acid) of mitochondrial genome of adult worms of small liver fluke from human in Nghe An (CsNA) and Dong Nai (CsDN) province were amplified. The nucleotide and amino acid of these sequences was comparatively analyzed with the known Clonorchis sinensis (Nam Dinh strain). The analysis revealed that the Nghe An Clonorchis sp and Dong Nai Clonorchis sp (in human) were homology (99.5 - 99.8% nucleotide) and similar (100% amino acid) to the Nam Dinh Clonorchis sinensis (identification was done). Thus, the Nghe An and Dong Nai Clonorchis sp were identified as Clonorchis sinensis in Opisthorchiidae family, Clonorchis genus.
Helminths ; Trematoda ; Human

During two years (2002-2004) a study was conducted in the Northern parts of Vietnam, 35 fascioliasis patients have been determined in 15 provinces. The clinical symptoms found in these patients were pain in the right quadrian of chest 94.3%, fever 60.0%, weight loss 57.1%, indigestion 54.3%, digestive disorder 42.9%, upper abdominal pain 42.9%, allergery 17.1%, out-penetration of adult worm from knee-joints 2.9%. The sub-clinical symptoms detemined by ultrasound images were liver abscesses with mixed echo (100%), eosinophillia in 34 patients (97.1%), Fasciola eggs in stool (25.7%), ELISA (+) with Fasciola gigantica antigen (100%) and almost by title of 1/12,800. Within those patients had been followed, some biochemical index of liver and kidney (GOT, GPT, Creatinin, Ure) were unchanged.
Fascioliasis ; Epidemiology ; Diagnosis

A male patient of 2,5 year old living in Vinh city, Nghe An province, who often had abdominal paine. On October 3rd, 2001, he has vomitted with 8 flukes, which were identified by morphology and lately confirmed by molecular approach in Institute of Biological Technology as Fasciolopsis buski
Fasciolidae ; disease ; diagnosis

Lung flukes Paragonimus heterotremus is a parasitic disease in which transmit by food, occur in 8 Northern mountainous provinces . The incidence of disease is from 0.3 to 15% on human, from 3.3 to 75% on dogs, from 8.7 to 98.1% on mountain scrab and from 1.4 to 3.6 % on snail. Clinical diagnosis based on mainly symptom such as haemoptysis or fluid pleurisy. Diagnosis definetely that have eggs of lung fluke in sputum, in fluid or in feces. Specific treatment medicine is praziquantel. Prevention of its disease by education communication for people and detective patients ealry then use specific treatment medicine
Lung ; Parasitic Diseases ; Pleurisy

Toan Son commune (Da Bac district, Hoa binh province) and Quy Huong commune (Moc Chau district, Son la province) are remote mountainous areas. Methods as interview, stool examination by Kato technique, sputum examination and snail examination by direct technique, crab muscle digestion were used in the study. Results of the study, showed that rate of people having habit of eating raw crab was 73.0% in Toan Son and 72.2% in Quy Huong; paragonimiasis rates were 3.3-11.3% and 3.4-15.7%, Ascaris infection rates was 52.3% and 50.0%, hookworm infection rates was 33.0% and 35.9%, Trichuris infection rates was 29.7% and 4.3%, clonorchiasis infection rate was 0.7 % (in Toan Son only), Taenia infection rates was 0.4% and 0.3%.In these communes, dogs, crabs and snails were also infected with paragonimus.
Helminthiasis ; Epidemiology

Vietnam is country with tropical climate, population community's life comform to habits, therefore parasitic infections, fungal infections in foods needed interest particularily. Foods infected helminth, germs through intermediary objects such as shellfishes, crabs, fishes, pigs,... because unhygienic foods infected to human. The author indicate that with contaminated route of helminth, unicell, fungus contaminated through foods, drink water, skin, breath,... depend on helminth's type that they exist and grow in human body and cause different diseases. To limit the contamination of germ, we must sure about food safety
Parasitic Diseases ; Food ; Helminthiasis

Sample of tapeworm was isolated from 1 patient in Ha Tay province. It was amplified by polymerase chain reaction (PCR), purified, cloned and sequenced. The nucleotide sequence was comparatively aligned with the known corresponding sequences of Taenia asiatica (Taiwanese); T. saginata (Chinese) and T. solium (Chinese and Vietnamese) and used for establishing a phylogenetic relationship for analysis of identification, using special programs. Molecular-based analysis revealed that the Taenia sp from this patient is identified as Taenia asiatica. Absolute nucleotide similarity (100%) between Taenia asiatica in Ha Tay, Vietnam and Taenia asiatica in Taiwan; the grouping together with T. asiatica (Taiwan) confirmed the sample obtained from Ha Tay is closely related to the Taiwanese T. asiatica
Taenia ; Centella ; Cestoda ; diagnosis ; Molecular Biology