Since 2002, adult worms of human small liver fluke in some provinces were identified by morphology and confirmed by molecular method as Clonorchis sinensis in Thanh Hoa, Ninh Binh, Nam Dinh and Bac Giang province; Opisthorchis viverrini in Phu Yen and Binh Dinh province. This study, using polymerase chain reaction (PCR), a portion of the cytochrome oxidase 1 (410 nucleotide and 136 amino acid) of mitochondrial genome of adult worms of small liver fluke from human in Nghe An (CsNA) and Dong Nai (CsDN) province were amplified. The nucleotide and amino acid of these sequences was comparatively analyzed with the known Clonorchis sinensis (Nam Dinh strain). The analysis revealed that the Nghe An Clonorchis sp and Dong Nai Clonorchis sp (in human) were homology (99.5 - 99.8% nucleotide) and similar (100% amino acid) to the Nam Dinh Clonorchis sinensis (identification was done). Thus, the Nghe An and Dong Nai Clonorchis sp were identified as Clonorchis sinensis in Opisthorchiidae family, Clonorchis genus.
Helminths ; Trematoda ; Human

During two years (2002-2004) a study was conducted in the Northern parts of Vietnam, 35 fascioliasis patients have been determined in 15 provinces. The clinical symptoms found in these patients were pain in the right quadrian of chest 94.3%, fever 60.0%, weight loss 57.1%, indigestion 54.3%, digestive disorder 42.9%, upper abdominal pain 42.9%, allergery 17.1%, out-penetration of adult worm from knee-joints 2.9%. The sub-clinical symptoms detemined by ultrasound images were liver abscesses with mixed echo (100%), eosinophillia in 34 patients (97.1%), Fasciola eggs in stool (25.7%), ELISA (+) with Fasciola gigantica antigen (100%) and almost by title of 1/12,800. Within those patients had been followed, some biochemical index of liver and kidney (GOT, GPT, Creatinin, Ure) were unchanged.
Fascioliasis ; Epidemiology ; Diagnosis

A male patient of 2,5 year old living in Vinh city, Nghe An province, who often had abdominal paine. On October 3rd, 2001, he has vomitted with 8 flukes, which were identified by morphology and lately confirmed by molecular approach in Institute of Biological Technology as Fasciolopsis buski
Fasciolidae ; disease ; diagnosis

Lung flukes Paragonimus heterotremus is a parasitic disease in which transmit by food, occur in 8 Northern mountainous provinces . The incidence of disease is from 0.3 to 15% on human, from 3.3 to 75% on dogs, from 8.7 to 98.1% on mountain scrab and from 1.4 to 3.6 % on snail. Clinical diagnosis based on mainly symptom such as haemoptysis or fluid pleurisy. Diagnosis definetely that have eggs of lung fluke in sputum, in fluid or in feces. Specific treatment medicine is praziquantel. Prevention of its disease by education communication for people and detective patients ealry then use specific treatment medicine
Lung ; Parasitic Diseases ; Pleurisy

Toan Son commune (Da Bac district, Hoa binh province) and Quy Huong commune (Moc Chau district, Son la province) are remote mountainous areas. Methods as interview, stool examination by Kato technique, sputum examination and snail examination by direct technique, crab muscle digestion were used in the study. Results of the study, showed that rate of people having habit of eating raw crab was 73.0% in Toan Son and 72.2% in Quy Huong; paragonimiasis rates were 3.3-11.3% and 3.4-15.7%, Ascaris infection rates was 52.3% and 50.0%, hookworm infection rates was 33.0% and 35.9%, Trichuris infection rates was 29.7% and 4.3%, clonorchiasis infection rate was 0.7 % (in Toan Son only), Taenia infection rates was 0.4% and 0.3%.In these communes, dogs, crabs and snails were also infected with paragonimus.
Helminthiasis ; Epidemiology

Vietnam is country with tropical climate, population community's life comform to habits, therefore parasitic infections, fungal infections in foods needed interest particularily. Foods infected helminth, germs through intermediary objects such as shellfishes, crabs, fishes, pigs,... because unhygienic foods infected to human. The author indicate that with contaminated route of helminth, unicell, fungus contaminated through foods, drink water, skin, breath,... depend on helminth's type that they exist and grow in human body and cause different diseases. To limit the contamination of germ, we must sure about food safety
Parasitic Diseases ; Food ; Helminthiasis

Sample of tapeworm was isolated from 1 patient in Ha Tay province. It was amplified by polymerase chain reaction (PCR), purified, cloned and sequenced. The nucleotide sequence was comparatively aligned with the known corresponding sequences of Taenia asiatica (Taiwanese); T. saginata (Chinese) and T. solium (Chinese and Vietnamese) and used for establishing a phylogenetic relationship for analysis of identification, using special programs. Molecular-based analysis revealed that the Taenia sp from this patient is identified as Taenia asiatica. Absolute nucleotide similarity (100%) between Taenia asiatica in Ha Tay, Vietnam and Taenia asiatica in Taiwan; the grouping together with T. asiatica (Taiwan) confirmed the sample obtained from Ha Tay is closely related to the Taiwanese T. asiatica
Taenia ; Centella ; Cestoda ; diagnosis ; Molecular Biology

Molecular examination on Taenia solium cysticercus isolated from Vietnamses patients in Bac ninh province. Total DNA was extracted. PCR were performed and the nucleotide sequences were developed. Nucleotide sequences and amino acids of cob gene fraction was confronted to some species of Taenia isolated from China, Taiwan. The result confirmed that cysticercus isolated from Vietnamese is Taenia solium
Taenia ; Taenia solium ; Cysticercus ; Molecular Biology ; diagnosis

4826 stool samples collected from 5 communes were examined by Kato- Katz technique. The infection rate of clonorchis sinensis was 33,9% in average (22,5% to 37,3%). The infection rate in male was higher than in female by 4 times and highest in 40- 49 age group (51,9%). The habit of eating raw fish is very common in this area (55,6%- 80,4%). The adult worms from patients in these study sites were identified as clonorchis sinensis
Clonorchis sinensis ; epidemiology ; Fishes ; Helminths

The survival ability of the larve of Clonorchis sinensis in raw fish dish prepared by traditional culinary method in NghiaLac commune, NghiaHung District,NamDinh province and the larve of Paragonomus heterotrimus in grilled crabs processed in KhanhHoa commune, LucYen district, YenBai province was investigated..In this raw fish dish, 93-95% of the microcerca of Clonorchis sinensis and in this grilled crab, 23,3-65% of microcerca had been living
Fishes ; Clonorchis sinensis ; epidemiology