The investigation was performed in 20 cadavers (dissection in 17 formaldehyde preserved and dye injection in 3 fresh ones). The results of study showed that: average, each rectus abdominal muscle has 4.97 vessel with 0.5-0.6 mm in diameter arising from the anterior gone up through the sheath of abdominal muscle. Among them, 4.56 arising from the deep inferior epigastric artery (DIEA) and they concentrate mainly in the paraumbilical area (87.7%). The cutaneous blood supply (dye coloured area) of the DIEA was 19.5 x 14 cm in the supra and infra umbilical regions. The DIEA and the vein form a suitable pedicle for free tissue transfer. The design of different skin flaps patterns was proposed by results.
Surgical Flaps ; Surgery

Background: Pesticides have been used widely in not only around the world but also Vietnam; however, their misuse can cause many serious health problems. For this reasons, it is necessary to closely monitor the use of pesticides and their effects on the health of farmers. Objectives: To describe the kinds of pesticides, the symptoms and related factors of acute pesticide poisoning among flowers planters. Subjects and method: A cross-sectional analytic design was applied with a sample of 107 households in the Hong Ha 1 and Hong Ha 2 village of My Tan commune, My Loc district, Nam Dinh province from November 2005 to February 2006. \r\n', u'Results: There were various types of pesticide that were being sprayed in the flower planting villages. Almost all types were moderately hazardous. 85% of farmers experienced at least 1 symptom of acute poisoning after spraying pesticides. The most common symptoms were headache, dizziness, rash (accounting for 45-58%), followed by red eyes and musculoskeletal symptoms (approximate 40%). The effects of pesticides to the farmer\u2019s health were proved with the typical symptoms of toxicity, especially headache, irritation of the face, red eyes. The percentages of self-reported toxicity symptoms were associated with sex, age groups, following manual and pesticide exposure. Conclusion: The results suggested that various interventions is required in order to change the knowledge, attitude and practice of pesticide use among farmers in general and flowers planters in particularly.\r\n', u'
pesticide ; acute pesticide poisoning ; flowers planter

Background: Pesticides safety, plant protection chemicals are widely used around the world, especially in agriculture to protect crops. \r\n', u'Objectives: To describe the real statute of knowledge and practice of safe pesticide use among flowers planters and various other related factors. \r\n', u'Subjects and method: The cross sectional analytic study was carried out in 2006 to evaluate the current situation of knowledge and practice of safe pesticide use in 2 flower planting villages: Hong Ha 1 and Hong Ha 2, My Tan commune, My Loc district, Nam Dinh Province. Results:The results showed that knowledge on determining toxicity levels and the absorption routes of pesticides to the human body through land and water is insufficient. As for pesticide use practice, the percentage of planters fully utilizing safety work clothes and other equipments was low. Multivariate analysis found that there was a significant correlation between good practice and knowledge. In addition, some predictors of good knowledge were: highly educated, male and age. Conclusion: The findings also discovered the priority of improving direct communication on safe pesticide use among planters. \r\n', u'
safe pesticide use ; flowers planters

Background/Introduction:The proportion of TS \u2013 Q96 ranges from 1/1500 \u2013 1.300 female newborns and about 3% of fetuses. In most of the world, TS can be diagnosed and treated at the early stages of pregnancies. In Vietnam, TS patients are frequently detected at the later stages with serious syndromes. TS diagnosis mainly relies on chromosomal analysis of amnion cells. Thus, prenatal diagnosis of TS is the rationale of this study.\r\n', u'Objectives: Utilize chromosomal analysis and FISH methods to diagnose Turner syndrome from amnion cells. \r\n', u'Subject and method: 30 pregnancies (from week 14-22) with high risks of TS, which were detected by ultrasound scan and triple test, 15 mil amnio fluid is withdrawn for the FISH technique from interphase amniocytes and amnio cultures, chromosomal analysis from metaphase cultured cells. \r\n', u'Results/Outcomes: Chromosomal analysis and FISH analysis give the same results: - 12/30 fetus with TS, 5/30 fetus with normal female results, \u2013 4/30 fetus with normal male normal results, \u2013 4/30 fetus with Down syndrome, \u2013 5/30 fetus with Edward syndrome. 11/12 TS fetus have large cystic hygromas, 9/11 cystic hygromas are separated. 12/12 TS fetus have triple test (+) with the threshold: APF \u2264 0.7 MoM, HCG \u2265 2 MoM, uE3 \u2264 0.7 MoM.\r\n', u'Conclusion:Chromosonal analysis and FISH are standards for diagnosing TS fetus. FISH can provide a quick result (48-72h). \r\n', u'
Turner syndrome (TS) ; Chromosome ; Fluorescence in site hybridization (FISH)

Background: FISH can detect number and structural chromosome aberrations in DNA. FISH is new technique in Vietnam, we combine FISH with chromosome analysis to prenatal diagnosis Down syndrome and turner syndrome that are high rate in birth defect.Objectives: To detect Down syndrome and turner syndrome by using FISH technique with chromosome analysis from amniotic cell.Subjects and method: 14amniotic cells samples 15th - 20th week with high risk of birth defects. Advance using FISH and chromosome analysis from amniotic cell. Results: We obtained results as follow: - 14/14 samples: correspondence between FISH and chromosome analysis. \ufffd?Detected 2 Down syndrome (female. Trisomi 21) and 4 Turner syndrome (45, X). Conclusion: Detected Down syndrome and Turner syndrome by using FISH technique with chromosome analysis from amniotic cell.
Down Syndrome/ diagnosis ; Turner Syndrome/ diagnosis ; Predictive Value of Tests ; Prenatal Diagnosis ; In Situ Hybridization ; Fluorescence ;

Background: There are many methods used in epidemiological studies of tuberculosis (TB) bacteria but Spoligotyping method is widely used with high accuracy, simple procedure, and carried out on strains containing a little of IS6110 segment \r\n', u'Objectives: To improve Spoligotyping technique and apply this technique to classify Mycobacterium tuberculosis\r\n', u'Subjects and method: Subjects and methods: The study included 12 medical waste samples collected at Thai Binh Hospital of Tuberculosis and 19 samples obtained from the The Hanoi Institute for Tuberculosis and Lung Diseases. Spoligo model of 31 samples were analyzed based on Spotclust and SpoIDB4 database and divided up into family and subfamily.\r\n', u'Results: Spoligotyping technique has good results with the PCR product amplified 40 cycles and presented the film in 18 hours. Obtained results in 31 medical waste samples belong to 4 families: Beijing, EAI, T1 and H3-LAM9. EAI and Beijing are dominant families with 45.16% and 38.7%), respectively. T1 and H3-LAM9 are 12.9% and 3.22%, respectively. The number of samples in the study is little but the obtained rate of different spoligo models of strains are quite diversified (41.9%)\r\n', u'Conclusion: This result is relatively appropriate with previous studies on the distribution of EAI and Beijing families in Vietnam and the world. Spligotyping technique distinguished samples belonging to Beijing or non-Beijing families, which support for clinical treatment and development of new vaccines.\r\n', u'\r\n', u'\r\n', u'
Mycobacterium tuberculosis ; Tuberculosis

Background: Cystic hygromas is a common abnormal event in obstetrics ultrasound, which is induced by a chromosome disorder; it is also one of the major causes inducing fetus\u2019s congenital malformation. Objective: Determining chromosomal aberration in nuchal cystic hygromas by FISH technique and outcomes the value of factors in prognosis fetuses with cystic hygroma. Subject and methods: 53 fetuses with cystic hygroma, which are detected by ultrasound scan, are analyzed by FISH technique. Compare results of FISH, band G chromosomal analysis, ultrasonographic abnormalities, followed the fetuses. Results: Chromosomal and FISH analysis give the same detection: abnormal chromosomes: 75.46%, the highest rate is Turner syndrome: 50.94%, normal chromosome: 24.53%. Abnormal chromosomal fetuses: multi-malformation, grim prognosis. Cystic hygroma with other malformation in scan: high rate chromosomal aberrations and septated hygroma, Turner syndrome fetuses have large cystic hygroma, 4/6 fetuses with normal chromosome and without other abnormal result scan have resolutions of hygroma in the second trimester, normal birth. Conclusions: Abnormal chromosomes: 75.46%. Prognosis is grim: abnormal chromosomes, other malformations in scan, large cystic, septated hygroma. Prognosis is better: normal chromosomes, without other ultrasonographic abnormalities, small cystic, nonseptated hygroma, resolution of cystic hygroma.
cystic hygroma ; FISH technique ; chromosome

Background: According to WHO, there are 5 million deaths from kinds of injury a year in the world, of which 875000 deaths occur in children under 18 years old. Injury leads to 40000 deaths which account for 10.7% deaths due to all other causes a year in VietNam. Objective: To show conclusions of the situation of child injury, recommendations for prevention of child injury and methods in order to enhance emergency activity in Vietnam. Subjects and method: The authors collected information about all patients who had emergencies due to injury at Viet Duc hospital, from March 26th 2006 to Octorber 26th 2006. However, supervised cases were the patients under 18 years old who were examined and treated at the Department of Emergency. Results: During the period of study, 2536 patients under 18 years old were examined for injury, of which death and coming back home for death were 83 cases (3.27%). 974 children had emergency resulted from injury: male was more than female: 697 verus 227. The leading causes of child injury were fall and traffic accident, respetively 34% and 60%. The most common traumas were traumatic brain injury (45%), limbs injury (41%). Although most of cases had first aid at the hospitals in district or province level, the rate of non-first aid cases was high (34%). Conclusion: Child injury is a leading cause of child death in hospitals. Methods for prevention of child injury should focus on educating and propagandizing to minimize injuries caused by traffic accident and fall.
Wounds and Injuries ; Child

Curcuma zedoaria (Christm.) Rosc. is a popular traditional herb to treat digestive disorders in Asian tropical countries. Previous studies indicated the presence of sesquiterpenoids, diterpenoids, and curcuminoids with various bioactivities. To enrich the phytocomposition data of this plant, this investigation was conducted.The dried rhizomes of C. zedoaria were collected in Hai Phong City (Vietnam), extracted with methanol and fractionated with n-hexane, CH2Cl2 , and EtOAc. Compounds were isolated from n-hexane soluble fraction by open column chromatography combined with thin layer chromatography from fraction n-hexane. Their chemical structures were elucidated by 1D, and 2D NMR spectra and comparison with reported data. As a result, a phytochemical investigation was conducted to isolate six sesquiterpenes from C. zedoaria. Their chemical structures were elucidated to be curcumenol (1), procurcumenol (2), neoprocurcumenol (3), 13-hydroxygermacrone (4), zederone (5), and curcumalactone (6). Among isolated compounds, compounds 1, 2, 4, and 5 were reported from C. zedoaria. Meanwhile, neoprocurcumenol (3) and curcumalactone (6) are isolated from this species for the first time. Compound 5 exhibited a mild inhibitory effect on α-glucosidase with an IC50 of 99.45 ± 0.50 μg/mL.

Curcuma zedoaria (Christm.) Rosc. is a popular traditional herb to treat digestive disorders in Asian tropical countries. Previous studies indicated the presence of sesquiterpenoids, diterpenoids, and curcuminoids with various bioactivities. To enrich the phytocomposition data of this plant, this investigation was conducted.The dried rhizomes of C. zedoaria were collected in Hai Phong City (Vietnam), extracted with methanol and fractionated with n-hexane, CH2Cl2 , and EtOAc. Compounds were isolated from n-hexane soluble fraction by open column chromatography combined with thin layer chromatography from fraction n-hexane. Their chemical structures were elucidated by 1D, and 2D NMR spectra and comparison with reported data. As a result, a phytochemical investigation was conducted to isolate six sesquiterpenes from C. zedoaria. Their chemical structures were elucidated to be curcumenol (1), procurcumenol (2), neoprocurcumenol (3), 13-hydroxygermacrone (4), zederone (5), and curcumalactone (6). Among isolated compounds, compounds 1, 2, 4, and 5 were reported from C. zedoaria. Meanwhile, neoprocurcumenol (3) and curcumalactone (6) are isolated from this species for the first time. Compound 5 exhibited a mild inhibitory effect on α-glucosidase with an IC50 of 99.45 ± 0.50 μg/mL.