1.Inhibition of human cancer cell lines by recombinant trichobakin
Journal of Vietnamese Medicine 2003;284(5):26-30
Recombinant trichobakin (TBK) has been subjected to study its capacity in inhibiting the growth of three lines of human cancer cell - epidermoid carcinoma of the mouth (KB), rabdomyo sarcoma (RD-26) and myelo sarcoma (2/0-Ag-14), it has been shown that TBK has inhibited the cancer cells. Myeloma sarcoma cells were inhibited by TBK at the lowest dose (ID50 2.55 microgram/ml). For RD-26 and KB cell lines, ID50 = 7.27microgram and 10.5 microgram/ml respectively. After 2 year preservation, protein recombinant contains almost the same inhibition activity from its original one
Neoplasms
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Therapeutics
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drugs
2.Phenolic Constituents from Balanophora laxiflora with their Anti-inflammatory and Cytotoxic Effects
Nguyen Thi HONG ANH ; Nguyen Thuy DUONG ; Pham Duc VINH ; Do Thi HA
Natural Product Sciences 2021;27(1):49-53
Balanophora laxiflora Hemsl. (Balanophoraceae) is a traditional medicinal plant with a diverse array of biological activities. In our exploration of new bioactive constituents from B. laxiflora, we isolated five compounds, including a new lignan, balanophorone (5), and four known phenolic compounds (1–4). The chemical structures of these compounds were determined by extensive spectroscopic analyses, including 1D and 2D NMR, HR-ESI-MS, and CD. In addition, we evaluated the effects of each of the isolates (1–5) on the messenger RNA expression levels of tumor necrosis factor (TNF)-α and cyclooxygenase (COX)-2 in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages and cytotoxicity against MCF-7 and MDA-MB-231 breast cancer cells. Compound 2 showed significant inhibition of LPS-induced COX-2 and TNF-α expression in RAW 264.7 macrophages, while compound 4 showed moderate cytotoxicity against MCF-7 and MDA-MB-231 breast cancer cells, with IC 50 values of 18.3 and 30.7 μM, respectively. No significant effects on the viability of normal mammary epithelial cells were observed.
3.Phenolic Constituents from Balanophora laxiflora with their Anti-inflammatory and Cytotoxic Effects
Nguyen Thi HONG ANH ; Nguyen Thuy DUONG ; Pham Duc VINH ; Do Thi HA
Natural Product Sciences 2021;27(1):49-53
Balanophora laxiflora Hemsl. (Balanophoraceae) is a traditional medicinal plant with a diverse array of biological activities. In our exploration of new bioactive constituents from B. laxiflora, we isolated five compounds, including a new lignan, balanophorone (5), and four known phenolic compounds (1–4). The chemical structures of these compounds were determined by extensive spectroscopic analyses, including 1D and 2D NMR, HR-ESI-MS, and CD. In addition, we evaluated the effects of each of the isolates (1–5) on the messenger RNA expression levels of tumor necrosis factor (TNF)-α and cyclooxygenase (COX)-2 in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages and cytotoxicity against MCF-7 and MDA-MB-231 breast cancer cells. Compound 2 showed significant inhibition of LPS-induced COX-2 and TNF-α expression in RAW 264.7 macrophages, while compound 4 showed moderate cytotoxicity against MCF-7 and MDA-MB-231 breast cancer cells, with IC 50 values of 18.3 and 30.7 μM, respectively. No significant effects on the viability of normal mammary epithelial cells were observed.
4.An Outbreak of Trichinosis with Molecular Identification of Trichinella sp. in Vietnam.
Nguyen Van DE ; Nguyen Vu TRUNG ; Nguyen Hong HA ; Vu Thi NGA ; Nguyen Minh HA ; Pham Thanh THUY ; Le Van DUYET ; Jong Yil CHAI
The Korean Journal of Parasitology 2012;50(4):339-343
The 5th outbreak of trichinosis occurred in a mountainous area of North Vietnam in 2012, involving 24 patients among 27 people who consumed raw pork together. Six of these patients visited several hospitals in Hanoi for treatment. Similar clinical symptoms appeared in these patients within 5-8 days after eating infected raw pork, which consisted of fever, muscle pain, difficult moving, edema, difficult swallowing, and difficult breathing. ELISA revealed all (6/6) positive reactions against Trichinella spiralis antigen and all cases showed positive biopsy results for Trichinella sp. larvae in the muscle. The larvae detected in the patients were identified as T. spiralis (Vietnamese strain) by the molecular analysis of the mitochondrial cytochrome c oxidase subunit III (cox3) gene.
Adult
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Animals
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Antigens, Helminth/analysis/immunology
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*Disease Outbreaks
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Electron Transport Complex IV/genetics
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Enzyme-Linked Immunosorbent Assay
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Female
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Humans
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Larva
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Male
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Meat/*parasitology
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Mitochondria/genetics
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Muscles/parasitology/pathology
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Swine
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Trichinella spiralis/genetics/immunology/*isolation & purification
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Trichinellosis/*epidemiology/parasitology/pathology
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Vietnam/epidemiology
5.Result of production of standard Albumin from human plasma.
Phan Trung Do ; Duong Tuan Pham ; Hien Thi Do ; Ha Diem Vo ; Thuy Thi Nguyen ; Thin Duy Ngo ; Phuc Hanh Hoang ; Tri Anh Nguyen
Journal of Medical Research 2007;52(5):1-6
Background: Human albumin was produced and used in many countries. Cohn's technique had been used to precipitate albumin from human plasma. This technique was easy and cheap and the quality of the product was good. In Vietnam, human albumin had to import, but the prices was very expensive. Vietnam was having good plasma in large quantity and high quality. That\u2019s why research on production plasma albumin was essential.\r\n", u"Objectives: This study aimed at using Cohn's technique improved by Drohan and Van - Aken to produce standard albumin from human plasma. Subjects and method: Human plasma detected VIII-factor was used for present study. Plasma \ufffd?albumin was precipitated by ethanol at low temperature and pH. The collected albumins have been liophilizated and storage at 40C. The quality and quantity of Albumin was evaluated by quantitative analysis and protein \ufffd?electrophoresis. Results: The 418g of albumin powder was produced from 16 liters of plasma detected F \ufffd?VIII. The quality of this albumin come up to standard (>95%) and quantity of albumin collected from one liter of this plasma was 26g. Conclusion: In the Vietnamese condition, the technique of Cohn can be used to produce standard albumin for treatment.\r\n", u'
Albumins/ standards
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Plasma
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6.Optimization of the Extraction Process for Bioactive Compounds from the Root Barks of Moringa oleifera
Do Hoang GIANG ; Tran Van HIEN ; Nguyen Thi THU MINH ; Nguyen Thu UYEN ; Hoang Thuy DUONG ; Bui Thi NHAT LE ; Mai Thi THU HA ; Nguyen Tien DAT
Natural Product Sciences 2023;29(4):281-286
The optimal condition for Moringa oleifera root barks extraction was determined using response surface methodology and Box-Behnken Design. The actual optimal condition of the factors was 65 o C, ethanol 60%, 40 (mL/g) liquid-to-solid ratio with 240 minutes extraction time. The enrichment of phenolic compounds sharply affected the antioxidant, and inhibitions of α-amylase enzyme, as well as, the anti-inflammatory effect of the extract from M. oleifera root barks. The extract in the optimal condition exhibited better 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and α-amylase inhibitory activities than those of positive controls.Also, the extract showed weak hydroxyl free radical scavenging and nitric oxide (NO) production inhibitory effects. These revealed a simple and promising method for the preparation of bioactive products from the root bark of M. oleifera.
7.Development of Acellular Respiratory Mucosal Matrix Using Porcine Tracheal Mucosa
Soo Yeon JUNG ; An Nguyen-Thuy TRAN ; Ha Yeong KIM ; Euno CHOI ; So Jeong LEE ; Han Su KIM
Tissue Engineering and Regenerative Medicine 2020;17(4):433-443
BACKGROUND:
Respiratory mucosa defects result in airway obstruction and infection, requiring subsequent functionalrecovery of the respiratory epithelium. Because site-specific extracellular matrix (ECM) facilitates restoration of organfunction by promoting cellular migration and engraftment, previous studies considered decellularized trachea an idealECM; however, incomplete cell removal from cartilage and mucosal-architecture destruction are frequently reported. Here,we developed a decellularization protocol and applied it to the respiratory mucosa of separated porcine tracheas.
METHODS:
The trachea was divided into groups according to decellularization protocol: native mucosa, freezing–thawing (FT), FT followed by the use of Perasafe-based chemical agents before mucosal separation (wFTP), after mucosalseparation (mFTP), and followed by DNase decellularization (mFTD). Decellularization efficacy was evaluated by DNAquantification and hematoxylin and eosin staining, and ECM content of the scaffold was evaluated by histologic analysisand glycosaminoglycan and collagen assays. Biocompatibility was assessed by cell-viability assay and in vivotransplantation.
RESULTS:
The mFTP mucosa showed low antigenicity and maintained the ECM to form a proper microstructure.Additionally, tonsil-derived stem cells remained viable when cultured with or seeded onto mFTP mucosa, and the in vivohost response showed a constructive pattern following implantation of the mFTP scaffolds.
CONCLUSION
These results demonstrated that xenogenic acellular respiratory mucosa matrix displayed suitable biocompatibilityas a scaffold material for respiratory mucosa engineering.
8.Development of Acellular Respiratory Mucosal Matrix Using Porcine Tracheal Mucosa
Soo Yeon JUNG ; An Nguyen-Thuy TRAN ; Ha Yeong KIM ; Euno CHOI ; So Jeong LEE ; Han Su KIM
Tissue Engineering and Regenerative Medicine 2020;17(4):433-443
BACKGROUND:
Respiratory mucosa defects result in airway obstruction and infection, requiring subsequent functionalrecovery of the respiratory epithelium. Because site-specific extracellular matrix (ECM) facilitates restoration of organfunction by promoting cellular migration and engraftment, previous studies considered decellularized trachea an idealECM; however, incomplete cell removal from cartilage and mucosal-architecture destruction are frequently reported. Here,we developed a decellularization protocol and applied it to the respiratory mucosa of separated porcine tracheas.
METHODS:
The trachea was divided into groups according to decellularization protocol: native mucosa, freezing–thawing (FT), FT followed by the use of Perasafe-based chemical agents before mucosal separation (wFTP), after mucosalseparation (mFTP), and followed by DNase decellularization (mFTD). Decellularization efficacy was evaluated by DNAquantification and hematoxylin and eosin staining, and ECM content of the scaffold was evaluated by histologic analysisand glycosaminoglycan and collagen assays. Biocompatibility was assessed by cell-viability assay and in vivotransplantation.
RESULTS:
The mFTP mucosa showed low antigenicity and maintained the ECM to form a proper microstructure.Additionally, tonsil-derived stem cells remained viable when cultured with or seeded onto mFTP mucosa, and the in vivohost response showed a constructive pattern following implantation of the mFTP scaffolds.
CONCLUSION
These results demonstrated that xenogenic acellular respiratory mucosa matrix displayed suitable biocompatibilityas a scaffold material for respiratory mucosa engineering.
9.The potential efficacy of the E2-subunit vaccine to protect pigs against different genotypes of classical swine fever virus circulating in Vietnam
Ha Thi Thanh TRAN ; Duc Anh TRUONG ; Viet Duc LY ; Hao Thi VU ; Tuan Van HOANG ; Chinh Thi NGUYEN ; Nhu Thi CHU ; Vinh The NGUYEN ; Duyen Thuy NGUYEN ; Kohtaroh MIYAZAWA ; Takehiro KOKUHO ; Hoang Vu DANG
Clinical and Experimental Vaccine Research 2020;9(1):26-39
10.The Association of Cytokines with Severe Dengue in Children
Julia N.A. Mangione ; Nguyen Tien Huy ; Nguyen Thi Phuong Lan ; Evaristus Chibunna Mbanefo ; Tran Thi Ngoc Ha ; Lam Quoc Bao ; Cao Thi Phi Nga ; Vo Van Tuong ; Tran Van Dat ; Tran Thi Thuy ; Ha Manh Tuan ; Vu Thi Que Huong ; Kenji Hirayama
Tropical Medicine and Health 2014;():-
Background: Dengue virus infection is a major public health problem. A hypothesis put forward for severe dengue is the cytokine storm, a sudden increase in cytokines that induces vascular permeability. Previous studies and our recent meta-analysis showed that IL-6, IL-8, IFNγ, TNFα, VEGF-A and VCAM-1 are associated with dengue shock syndrome. Therefore, in this study we aim to validate the association of these cytokines with severe dengue. Methods & Findings: In a hospital based case control study in Vietnam, children with dengue fever, other febrile illness and healthy controls were recruited. Dengue virus infection was confirmed by several diagnostic tests. Multiplex Immunoassay using Luminex technology was used to measure cytokines simultaneously. A positive association with dengue shock syndrome was found for VCAM-1, whereas a negative association was found for IFNγ. Furthermore, the multivariate logistic analysis also showed that VCAM-1 and IFNγ were independently correlated with dengue shock syndrome. Conclusion: IFNγ and VCAM-1 were associated with dengue shock syndrome, although their role in the severe dengue pathogenesis remains unclear. Additional studies are required to further investigate the function of these cytokines in severe dengue.