In the flowers of Sambicus nigra, flavonoids, amino acids and sugars were determined. Among them the main components were flavonoids, which accounted for 7,19±0,10%. From total flavonoid extracts, ,2 compounds were isolated by column chromatography and identified by UV,MS,and NMR spectroscope as quercetin-3-α rhamnopyranosyl- β -glucopyranose and quercetin
Chemistry ; Flowers ; Flavonoids ; Amino Acids ; Carbohydrates

Study on the effect hypoglycemosis of extract from Sambucus nigra ssp. canadensis (L) R. Bolli flowers on streptozotocin-induced diabetic mice. A single daily dose of extract (equivalent to 0.4g flower/kg daily) were administered for 5 days or 10 days to the diabetic mice immediately following Streptozotocin injection. As a result, extract from Sambucus nigra ssp. canadensis (L) R. Bolli flowers inhibited glucose concentration in diabetic mice at the investigated dose. It is indicated that the flowers of Sambucus nigra ssp. canadensis (L) R. Bolli may be an effective agent for treatment of diabetes
Hypoglycemia ; Medicine, Traditional ; Pharmaceutical Preparations

Background: Salpingostomy for ectopic pregnancy in patients who has aspirations to having a baby is a necessity. Objective: To discover the factors affecting the outcome of salpingostomy. Subjects and method: 400 patients with none or one baby treated by salpingectomy or salpingostomy for ectopic pregnancy in National Hospital of Obstetrics and Gynecology from July 2006 to June 2007 were studied. The data was analyzed with T test and logistic regression. Results: 92 of 400 patients (23%) were treated by salpingostomy. The risk of salpingectomy for patients with size of pregnancy mass >2 cm (measured by ultrasound) was 2.48 times higher than that of mass <=2 cm (95% CI: 1.50-4.12). All of cases with positive fetal heart beat were treated by salpingectomy. The danger of salpingectomy for patients with preoperative level of bhCG >3000 UI/L increased by 6.65 fold in comparison with that of bhCG <=3000 UI/L (95% CI: 2.99-15.27). The risk of salpingostomy for patients with the size of pregnancy mass >3 cm is 7.43 times as much as that of mass <=3 cm (95% CI: 3.89-14.39). Conclusion: The chance of salpingostomy for patients having the size of pregnancy mass <=3 cm and bhCG <=3000 UI/L was 24.1%.
Ectopic pregnancy ; salpingostomy

Background: Viet Nam confirmed its first case of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection on 23 January 2020 among travellers from Wuhan, China, and experienced several clusters of community transmission until September. Viet Nam implemented an aggressive testing, isolation, contact tracing and quarantine strategy in response to all laboratory-confirmed cases. We report the results of SARS-CoV-2 testing during the first half of 2020 in northern Viet Nam. Methods: Between January and May 2020, 15 650 upper respiratory tract specimens were collected from 14 470 suspected cases and contacts in northern Viet Nam. All were tested for SARS-CoV-2 by real-time RT-PCR. Individuals with positive specimens were tested every three days until two tests were negative. Positive specimens from 81 individuals were cultured. Results: Among 14 470 tested individuals, 158 (1.1%) cases of SARS-CoV-2 infection were confirmed; 89 were imported and 69 were associated with community transmission. Most patients (122, 77%) had negative results after two tests, while 11 and 4 still tested positive when sampled a third and fourth time, respectively. SARS-CoV-2 was isolated from 29 of 81 specimens (36%) with a cycle threshold (Ct) value <30. Seven patients who tested positive again after testing negative had Ct values >30 and negative cultures. Conclusion Early, widespread testing for SARS-CoV-2 in northern Viet Nam identified very few cases, which, when combined with other aggressive strategies, may have dramatically contained the epidemic. We observed rapid viral clearance and very few positive results after clearance. Large-scale molecular diagnostic testing is a critical part of early detection and containment of COVID-19 in Viet Nam and will remain necessary until vaccination is widely implemented.

Objective To characterize viral co-infections among representative hospitalized measles cases during the 2014 Hanoi outbreak. Methods Throat swabs were collected from 54 pediatric patients with confirmed measles, and molecular diagnostics performed for 10 additional viral respiratory pathogens (Influenza A/H1N1pdm09; A/H3N2 and influenza B; Parainfluenza 1, 2, 3; Respiratory Synctial Virus, RSV; human Metapneumovirus, hMPV; Adenovirus and Picornavirus). Results Twenty-one cases (38.9%) showed evidence of infection with other respiratory viruses: 15 samples contained measles plus one additional virus, and 6 samples contained measles plus 2 additional viruses. Adenovirus was detected as a predominant cause of co-infections (13 cases; 24.1%), followed by RSV (6 cases; 11.1%), A/H1N1pdm09 (3 cases; 5.6%), PIV3 (3 cases; 3.7%), Rhinovirus (3 cases; 3.7%) and hMPV (1 case; 1.96%). Conclusions Viral co-infections identified from pediatric measles cases may have contributed to increased disease severity and high rate of fatal outcomes. Optimal treatment of measles cases may require control of multiple viral respiratory pathogens.

Objective: Human respiratory syncytial virus (RSV) is a primary cause of paediatric severe acute respiratory infection (SARI) worldwide, especially in developing countries. We investigated the genetic characteristics of RSV in northern Viet Nam to determine the prevalence and distribution of subtypes as well as the diversity and transmission patterns of genotypes. Methods: In two facilities, from January 2017 to December 2020, 1563 clinical specimens were collected from paediatric patients hospitalized with SARI and tested for RSV. Selected positive samples underwent sequencing analysis targeting the second hypervariable region of the G gene using next-generation sequencing. Results: The RSV positivity rate was 28.02% (438/1563 samples), and prevalence was highest in children aged <1 year (43.84%; 192/438). Subtype RSV-A accounted for 53.42% (234/438) of cases, RSV-B for 45.89% (201/438), and there was coinfection in 0.68% (3/438). Both subtypes cocirculated and peaked during August–September in each year of the study. Phylogenetic analysis showed that RSV-A samples belonged to the ON1 genotype, which has three subgenotypes: ON1.1, ON1.2 and ON1.3. However, we did not find the 72-nucleotide duplication in the second hypervariable region of the G gene, a characteristic of genotype ON1, in any RSV-A samples. RSV-B samples belonged to genotype BA9. Discussion: Our results provide additional molecular characterization of RSV infections in Viet Nam. Specially, our study is the first to report the absence of the 72-nucleotide duplication in the G gene of RSV-A genotype ON1 in Viet Nam, which may help in understanding the genetic evolution of RSV and be useful for vaccine development in the future.