The study on using dispase and trypsine 0.25% to detach cells from fetal monkey kidneys of Macaca mulatta showed that: the detach ability of dispase was much higher than trypsin. Some characteristics of dispase were suitable for detaching fetal cell in cold condition or serum condition. However, dispase has not been used as commonly as trypsin. Dispase can not be a good solution to detach cells without the effect of trypsin
Endopeptidase ; cells ; Kidney ; Haplorhini ; Fetus

Quantitive assessments of serum cTnl, CK-MB in 106 coronary bypass graft (CABG) patients at base line after anesthesia induction; at the end of operation, and at every 6 hours during the first 24h post-operation and then, on 2nd, 3rd, and 5th postoperative day. The serum cTnl and CK-MB significantly increased from the end of the surgery to 5th postoperative day with the peak value (50.82 ± 31.72 mg/ml and 70.78 ± 64 UI/ml, respectively) reached on average 8-10h after the aortic unclamping. The aortic cross-clamping time correlated slight positively to the pear of the serum cTnl and CK-MB on the first postoperative day. The finding showed that the postoperative increase of cTnl levels in the CAGB patients resulted from several factors. The most important contributing factor was the cross clamping time, other ones were the cardioplegia types
Coronary Artery Bypass ; Cardiopulmonary Bypass

Study on 198 cases of coronary artery bypass surgery at Heart Institute of Ho Chi Minh city. There were 146 males and 52 females; mean weight: 59.46±9.37kg, mean age: 61.51±8.20 years old, the mean time of cardiopulmonary bypass: 155.07±36.28 minutes, the mean time of aortic clampage: 93.36±23.09 minutes, average number of coronary artery bypass grafts: 3.23±0.86, the mean time of extubation: 8.26±1.97 hours, the mean day of ICU length stay: 1.8±2.3 days, the mean day of hospital length stay: 17.93±8.5 days. These findings showed that the introduction of new anesthetic drugs with fast effectiveness and short duration of anesthesia could help to have rapid intubations, hemodynamic stability, and early extubation, to ensure patients’ safety, even in emergency operations or cardiac dysfunction patients
Coronary Artery Bypass ; Anesthesia

Dihydroxocisplatin [Pt(NH3Cl)2(OH)2] or compound of platin (IV)) has been synthesized and estimated its antitumor effectiveness on Swiss mice that affected with sarcoma TG180 ascitic tumor. The results showed that the compound of Platin(IV) reduced GR(%) to 14.7 (means inhibited 85.3% tumor development). The mitotic index of the tumor cells in experiments was twice lower than in control group
Neoplasms ; mice ; therapeutics ; Pharmaceutical Preparations

Background: Thoracoscopy has used for lots of thoracic disorders. Pneumothorax may be ideally suited for thoracoscopic management. Objective: To study the value of thoracoscopy in the diagnosis and treatment of pneumothorax. Subjects and method: From September 2003 to October 2006, 30 patients with pneumothorax were treated by thoracoscopy at Department of Respiratory of Bach Mai Hospital. This was a longitudinal, retrospective and prospective study. Prospective study included 15 patients from September 2005 to October 2006. Retrospective study included 15 patients from September 2003 to August 2005. Results: In 30 patients, primary spontaneous pneumothorax accounted for 80%, secondary spontaneous pneumothorax 13.3%, catamenial pneumothorax 3.3% and iatrogenic pneumothorax 3.3%. Mean age of patients was 34\xb115 years (ranged from 17 to 77 years). 90% of cases were male. Bullea was found in 70% by thoracoscopy. Patients were in stage I 13.3%, stage II 20%, stage III 33.3%, and stage IV 33.3%. There were 3 procedures with ligation, 11 procedures with coagulator, 25 procedures with talc poudrage; mean dose of talc was 8.2\xb12.2 g. The success rates of procedures with talc poudrage were 80% and 100% after supplemental pleurodesis. Complications and side effects were subcutaneous emphysema 46.7%, empyema 3.3%, high fever 3.3% that we thought was nosocomial infections, bleeding in pleural cavity 3.3%, chest pain 96.7%, fever 63.3%, dyspnea 10%. After a mean follow up of 14.1\xb110.4 months, the recurrent rate was 3.3%. Conclusions: Medical thoracoscopy achieved a high success rate in the diagnosis and treatment of pneumothorax.
Pneumothorax/ diagnosis ; therapy ; Thoracoscopy

Background: Pulmonary tuberculosis is a contagious bacterial infection that mainly involves the lungs, but may spread to other organs. Most people who develop symptoms of a tuberculosis infection first became infected in the past. However, in some cases, the disease may become active within weeks after the primary infection. Objective: To evaluate the diagnosis and treatment of pulmonary tuberculosis. Subjects and method: A retrospective study included 196 patients with pulmonary tuberculosis hospitalized in Department of Respiratory of Bach Mai Hospital in 2 years (2002 - 2003). Patients\ufffd?information of medical records was collected. Results and conclusions: 60.7% of patients were male and 39.3% were female. Tuberculous pleurisy was 59%, parenchymal lung tuberculosis 36%, and the others 5%. Clinical signs and symptoms included chest pain 67.9%, dyspnea 61.2%, cough 72.9% (nonproductive cough: 42.3%; productive cough: 30.6%), fever 61.2%. Dull to flat percussion, decreased to absent breath sound and fremitus 65.3% (89% tuberculous pleurisy). 27.6% of patients had positive AFB evident (smear, bronchial lavage fluid). The first line drugs were usually used in treatment of tuberculosis.
Tuberculosis ; Pulmonary/ diagnosis ; therapy

Background: Standard treatment for pleural infection includes of drainage and antibiotics. Chest tube drainage often fails if the fluid is loculated by fibrinous adhesions. Intrapleural fibrinolysis may facilitate pleural drainage. Objective: To evaluate the role of Streptokinase (STK) in the treatment of empyema and exudative pleural effusions. Subjects and method: A study included 34 patients (21 patients with exudative pleural effusions, 13 patients with empyema), aged 15-77 years. All patients received intrapleural STK daily with dose of 300.0000-500.000 UI for empyema and 300.000 UI for exudative pleural effusions. Response was assessed by clinical outcome, pleural fluid drainage, chest radiography, pleural ultrasound. Results: Mean pleural fluid drainage after STK instillation was higher in all patients. Pleural fluid drainage was 640 \xb1 494 ml before STK instillation and 823 \xb1 755 ml after STK instillation for empyema; 765 \xb1 691 ml before STK instillation and 1,033 \xb1 757 ml after STK instillation for exudative pleural effusions. The success rate of clinical outcome was 100%. Only 2 patients required decortications. Fever occurred in 3 patients (8.8%) and allergy in 1 patient (3%). Conclusions: Intrapleural STK was safe and effective in the treatment of empyema and exudative pleural effusions. STK prevents pleural adhesions and reduces the risks for surgery.
Streptokinase/ therapeutic use ; Empyema ; Pleural/therapy ; Pleural Effusion/ therapy

Background: To improve the physical fitness of athletes is one of the essential requirements of the Vietnam Sports associations. Research to discover the functional foods to improve the physical fitness of athletes in general, in particular athletic athletes is an interest to scientists. Objective: Evaluation of effect of Phunamine on some of the male, female athlete\u2019s biomedicine and physical fitness standards. Subject and methods: A controlled, double-blind, randomized, clinical experiment was conducted on 30 athletes of the Hanoi Athletic Group. They were then divided into 2 groups: research group: 20 athletes (10 men, 10 women) were administered with 4 phunamine pills per day; control group: 10 athletes (5 men, 5 women) were each administered with 4 placebo pills per day. The 2 groups had tests performed and followed up 30 days pre and post administration. Results: Max-min blood pressure change between the two groups was not significant (t(\u03b1)>0.5). Testosterone concentration change and physical fitness test (run 100m, 400m) of Phunamine dosed group were higher than the placebo group (t(\u03b1)<0.5). Conclusion: Phunamine has the effects of increasing testosterone concentration and improving physical fitness of athletes however the drug has no effect on blood pressure index of athletic athletes.
Phunamine ; functional foods ; athletes

Background: The fitness of athletes is always the top priority of each coach. Therefore, studying to discover the products that are really effective and appropriate for each sport is an urgent matter. Previous research showed biochemical standard is one of the important standards to assess the fitness of athletes. \r\n', u'Objectives: To study the effects of Phunamine on various biochemical standards of male and female weight-lifters. \r\n', u'Method: The self-control clinical experiment was applied in 19 weight-lifters at the Sport University 1 (TuSon) from May to July 2006. The research material was the functional food Phunamine with major component being anchovy combined with Syngnathiformes powder. Results: Testosterone concentration after taking Phunamine was higher than before the research (t(alpha)<0.025 in males; <0.01 in females); activity of LDH enzyme after taking Phunamine was higher than before the research, but the significant difference was only found in female with t(alpha)<0.025; cortisol concentration was also higher than before the research. \r\n', u'Conclusions: Phunamine has the effect of increasing testosterone, cortisol concentration and LDH enzyme activity.\r\n', u'
Phunamine ; biochemical standards

Background: Chronic obstructive pulmonary disease (COPD) remains a major public health problem. It is the fourth leading cause of chronic morbidity and mortality in the United States, and is projected to rank fifth in 2020 in burden of disease caused worldwide. Objective: To research the epidemiology of COPD in the population of Bac Giang city and finding out the risk factors of COPD. Subjects and method: Cross-sectional survey of general population sample of 2,104 men and women with the age \ufffd?0 years in 30 wards of 11 communes of Bac Giang city. Data on respiratory symptoms, diseases, and risk factors were collected. Lung function tests were performed with Spiroanalyzer snoo, Japan. Results and conclusions: The incidence of COPD was 2.3%; in men 3.0% and in women 1.7%. The incidence of simple chronic bronchitis was 6.4%. People who smoked had higher rate of COPD than the others (OR = 2.8). The incidence of smoking in COPD was 55.9%. The three most important risk factors of COPD was the age \ufffd?0; smoking >15 pack/year and medical history of asthma.
Pulmonary Disease ; Chronic Obstructive/ epidemiology