An uncontrolled clinical trial was conducted to investigate the effect of Naltrexone and Cognitive Behavioral Therapy (CBT) on relapse prevention in Green Center, Hochiminh city from October 2002 to August 2003. The sample included 106 addicts (94.3% males) who just passed the acute detoxification process. Patients came to the Green Center 3 times per week (every other day), took 100 mg Naltrexone (150mg on weekend) at the Center, and then got a CBT session in one hour. The Naltrexone-CBT program was prolonged in 6 months. The results: 65/106 (61.3%) patients have terminated the treatment course, in which, only 4 patients (6.2%) followed the whole program (6 months); 7 patients (10.8%) have followed 3 - 6 months. The average of treatment duration was 56.7  51.8 days. There were 29.2% addicts have relapsed, in which the relapse rate of addicts, who have followed the whole program in 6 months was 0%; of addicts who had the treatment duration from 3 – 6 months was 5.3%; and of addicts with the treatment duration less than 2 months was 89.4%. The main recognized reasons for the great drop-out rate were the familial interference which a tendency of shortening the treatment course just after 3 months abstinence of their offspring (27.7%), the high cost of the treatment in comparison to the average income of most people (18.5%), the treatment center is too far from addicts home (16.9%)
Naltrexone ; Cognitive Therapy ; prevention & control

Evaluation of the presence and resistance of E.coli in some specimens of surgical infection in Viet Duc Hospital, Military Hospital No.103 and Central Military Hospital No.108. The results showed that: E.coli was present in 23% of surgical infection’s specimens. Among 310 E.coli strains isolated from these specimens, the distribution of E.coli was different: E.coli in bile solution was 28.1%, in urine and pus were 21.3%. E.coli isolated from other specimens accounted low rate. Surgical infection induced E.coli was high sensitivity with netilmycine, amikacine, cefotaxime, gentamicine, norfloxacine and high resistance to ampicilline, co-trimoxazol, chloramphenicol and tetracycline.
Surgical Wound Infection ; Escherichia coli

The study was conducted in 47 patients (34 males, 13 females) with coronary artery disease or suspected coronary artery disease who were underwent SBSE from May 2002 to October 2004 at 115 People Hospital. Results: CAD was presented in 22 patients: among which, 7 cases with lesion of one major branch, 7 cases with two branches and 8 case with three branches of coronary artery. SBSE test was positive in 20/22 patients, overall sensitivity of 91%, specificity of 80% and accuracy of 85%. The sensitivity was greatest for the font interventricle artery (81%), compared to sensitivity for right coronary artery and the left coronary artery was 56% and 31%, respectively (p<0.005). For identifying patients with multivessels CAD, sensitivity 55%, specificity 97% and accuracy 83%. SBSE is a useful tool for evaluating CAD, and identifying both the location and extent of disease.
Coronary Artery Disease ; Diagnosis

Background: Umbilical cord blood - Stem cell CD34 are attracted to study and apply to cure many serious diseases. But the quantity of the cell is small, so it only used to children. Therefore, studying to find out the high effect collected process is necessary. Objective: studying to find out the high effect process of collecting hematopoietic stem cell from umbilical cord blood. Subjects and methods: the study included 112 umbilical cord blood specimens collected from healthy mother delivering at National Hospital of Obstetric and Gynecology.Results: The condition of the mother was healthy, with no risk factors of infection or genetic disease. The average age of mothers was 28 \xb1 5 years old, average weight: 63 \xb1 5 kg. Age of fetus was from 39-41 weeks, average weight: \ufffd?3000g. Collection technique: cutting the umbilical cord after delivery 6-10 seconds, cutting the umbilical cord far from neonatal 6 cm, blood collection continued after the collection of placenta, all processes must be implemented rapidly in the 4 -5 minutes in aseptic condition. With the above process it can be obtained with high effect blood: volume was 90 - 120ml (48%). CD34 cell count: 1.6 \xb1 0.3x106, the number of eukaryotic cells: 13.53 \xb1 1.7x108/unit of the umbilical cord. Conclusions: The process can take the large volume of umbilical cord blood, which can be used for children and low weight adult. \r\n', u'\r\n', u'\r\n', u'
Fetal Blood

Background: Pulmonary tuberculosis is a contagious bacterial infection that mainly involves the lungs, but may spread to other organs. Most people who develop symptoms of a tuberculosis infection first became infected in the past. However, in some cases, the disease may become active within weeks after the primary infection. Objective: To evaluate the diagnosis and treatment of pulmonary tuberculosis. Subjects and method: A retrospective study included 196 patients with pulmonary tuberculosis hospitalized in Department of Respiratory of Bach Mai Hospital in 2 years (2002 - 2003). Patients\ufffd?information of medical records was collected. Results and conclusions: 60.7% of patients were male and 39.3% were female. Tuberculous pleurisy was 59%, parenchymal lung tuberculosis 36%, and the others 5%. Clinical signs and symptoms included chest pain 67.9%, dyspnea 61.2%, cough 72.9% (nonproductive cough: 42.3%; productive cough: 30.6%), fever 61.2%. Dull to flat percussion, decreased to absent breath sound and fremitus 65.3% (89% tuberculous pleurisy). 27.6% of patients had positive AFB evident (smear, bronchial lavage fluid). The first line drugs were usually used in treatment of tuberculosis.
Tuberculosis ; Pulmonary/ diagnosis ; therapy

Background: The reduction of erythrocytes from cord blood is very need for long - term storage of C034 cells for transplantation. Reduced erythrocyte will reduces preservative blood volume, preservatives and freely HST when defrosting, so stem cells are better protected. Objectives: To study selection of the best centrifugal procedure to reduce maximal erythrocytes and lose minimal C034 cells from cord blood. Subjects and methods: 20 blood samples selected from 60 cord blood units was used for this study. The study was carried out through two steps. In the first step, the centrifugal speed was fixed and the centrifugal time was changed.In the second step, the centrifugal time was fixed, the centrifugal speed was changed. From collected results the best appropriate procedure to reduce erythrocytes from cord blood have been selected. Results: The procedure of gradient centrifuge with speed of 500g in 6 minutes isolated> 50% of erythrocytes, kept > 84% of CD34 cells and then centrifuge of 1000 g in 10 minutes reduced about 40% of volume of nuclear cell - suspension. Conclusion: The procedure can use for preparation of stem cell suspension from cord blood to storage in nitrogen liquid. \r\n', u'\r\n', u'
Erythrocytes/ pathology ; Fetal Blood/ chemistry ; drug effects ; immunology

Background: In Vietnam, there are a number of studies on the application of ATRA in treating acute promyelocytic leukemia (AML \u2013 M3) but they have still faced with certain difficulties. Objectives: (1). Study PML/RAR alpha fusion gene on the patients diagnosed with AML \u2013 M3. (2). Study the index of hematology of the PML/RAR alpha positive group. Subject and Method: 21 patients with acute promyelocytic leukemia (M3) were studied. All patients were examined with morphology, coagulation and cytogenetic tests and RNA were extracted from leukemic cells and PCR for PML/RAR alpha fusion transcript. Result and conclusion: PML/RAR alpha positive in 67% including 4 patients which were not discovered t(15; 17) by cytogenetic technique. Rates of three subtype (bcr1, bcr2 and bcr3) of PML/RAR alpha were 7 patients (50%), 3 patients (21,5%) and 4 patients (28,5%), respectively. WBC and bone marrow cells of PML/RAR alpha positive group were 5.08+/-3.87 and 155.82+/-106.21. D \u2013 Dimer level was 1954.89+/-1575.28; 93% of patients in the PML/RAR alpha positive group had DIC.
Acute promyelocytic leukemia ; M3 ; PML/RAR alpha

Background: Chromosome mutation type t(8;21) has quite a high frequency in acute myelogenous leukemia, which accounted for about 15% among adult patients. From 2001, the WHO has a new classification for acute myelogenous leukemia based on genetic mutations. Form had AML1/ETO were arranged into genetic mutation group with better prognosis and ability to fully recover after chemotherapy with a high dose of cytarabin. Objective: Study AML1/ETO fusion gene on the patients diagnosed with Acute Myelogenous Leukemia (AML), as well as the clinical features and some haematologic parameters of the AML1/ETO positive group. Subject and methods: 76 patients with AML were treating in the National Institute of Hematology & Blood Transfusion and the Department of Hematology & Blood Transfusion of Bach Mai Hospital from April 2007 to July 2008. These patients were studied for clinical examination, morphology and RNA were extracted from leukemic cells and PCR for AML1/ETO fusion transcript was performed. Results and conclusions: The incidence of AML1/ETO positive in the AML patients was 24%. The incidence of AML1/ETO positive in AML-M2 was 28%. In the AML1/ETO positive group: median age was 26.94+/-9.22; rate of severe anemia, hemorrhage, fever, infection, hepatomegaly, splenomegaly, lymphadenopathy and gum hypertrophy was 44%, 33%, 28%, 11%, 44%, 28%, 17% and 6%, respectively. Median hemoglobin, WBC, platelet, bone marrow cell count, % blast in peripheral blood and in bone marrow was 84.41+/-28.97 g/l, 29.42+/-31.36 g/l, 42.12+/-33.83 g/l, 215.93+/-134.42 g/l, 56.21+/-26.58% and 65.14+/-16.12%, respectively.
acute myelogenous leukemia ; AML1/ETO fusion gene

Based on our previous study, we evaluated the modulatory effects on LPS-induced IL-1β and IL-10 cytokine production in RAW 264.7 macrophages of several medicinal herbs, including P. scandens. The results showed that P. scandens extract showed significant effects on LPS-induced IL-1β and IL-10 cytokine production in RAW 264.7 macrophages. Therefore, in the current research, we focused on the P. scandens sample. Cytokine production effects bioassay-guided isolation of ethyl acetate fraction of 70% ethanol extract from roots of Paramignya scandens (XL) obtained seven coumarins (1–7). Their chemical structures were identified using spectroscopic methods (NMR and MS) and compared with those previously published data to be xanthyletin (1), luvangetin (2), clausenidin (3), nordentatin (4), dentatin (5), clausarin (6), and anisocoumarin E (7). This study represents the first report on the presence of compounds 3, 6, and 7 in the Paramignya genus and compounds 1 and 2 in XL. All isolates (1–7) exhibited significant inhibition of LPS-induced interleukin (IL)-1β production compared to the LPS 5 ng/mL control group, with IL-1β concentrations ranging from 42.77 to 69.76 pg/mL. Additionally, the IL-10 production induced by compounds 1‒7 in LPS-stimulated RAW 264.7 macrophages ranged from 175.98 to 321.56 pg/mL, demonstrating a marked increase as compared to the LPS 5 ng/mL control group. The stimulatory effect on IL-10 production and inhibitory effect on IL-1β production of compounds 1, 2, and 6 gradually increased with the test concentration in both RAW 264.7 macrophages and LPS-induced RAW 264.7 macrophages. Compounds 1, 2, and 6 inhibited IL-1β production in LPS-induced RAW 264.7 macrophages with IC 50 values of 10.70 ± 1.18 µM, 8.57 ± 1.05 µM, and 17.43 ± 1.05 µM, respectively. These findings highlight the potential of all the compounds derived from P. scandens roots in inducing IL-1β and IL-10 cytokines activity in LPS-stimulated RAW 264.7 macrophages. The results contributed to expanding the knowledge of the chemistry and bioactivities of P. scandens and provided valuable data for future investigations on this species.

Background: HIV/AIDS pandemic really become danger to mankind on the earth. Objectives: Description epidemiological characteristics of HIV/AIDS patients. Studying clinical expression, learning aboutdemands of infected patients and community care for infected patients. Subjects and method: 71 HIV/AIDS people in Thua Thien Hue were studied in 2006. Using descriptive method to give epidemiological characteristics and behaviour of HIV/AIDS people. People were determined HIV/AIDS infection based on the criteria of Ministry of Health. The information were collected by target study. Results: HIV/AIDS people mainly from the age of 20 to 39 (83,10%), males were 52,10% and in various levels of education and all kinds of occupation. The rate of sexually transmitted infection was 84,5% and infection transmission was 15,5%. The rate of sexually transmitted infection within the last 12 months was 57,7%. 63,4% of infected people used condom while having sex with their spouses or partners. 1,4% of infected people using drug infection within the last month. 38% of the infected people had manifestations of AIDS, 77,8% of HIV/AIDS were treated with antiviral drugs. Families and communities had positive attitudes and behaviors to the HIV/AIDS people. Infected people being remoted from the society was 12,7%, 88,7% of them receiving supports from their communities such as disease treatment, spirit and material supports and job opportunities. Conclusion: Strengthen communication activities to change behavior of HIV/AIDS people and in communitiy to reduce alienation, discrimination for infected people. Execute socialization in care and support for HIV/AIDS people.
HIV/ immunology ; isolation & ; purification ; pathogenicity ; Community Health Services/ organization & ; administration ; Epidemiologic Methods ;