The samples of dental deposit plaque collected from 30 people in the faculty of Natural Science were stained by Gram technique. A bacterial prosperious component was detected including Gram (+) cocci, Gram (-) cocci, red and thin cell, red and long cell, straight and curved Gram (-) cells, thin spinal cells … Microorganism population on the deposit plaque under the gun includes bacteria, cocci, and spirochaetes. 3 genera were determined including Streptococcus, Lactobacillus and Actinomyces, with a highest incidence of Streptococcus. The representative genus has the characteristic similar to Streptococcus mutans and it seems that it plays an important role leading to caries
Dental Plaque ; Bacteria ; Dental Care

Which component of polyphenol of Garcinia mangostana L effecting on S. mutans GS-5 causing tooth decay was studied. The materials: the microorganism strain Streptococus mutans GS-5 of America. The method: compounds of polyphenol were isolated by thin layer chromatography method. To measure the pH reduction in glycolysis. The result: Using thin layer chromatography on silicagen was isolated 15 bands of flavonoid from the extract of mangostana. The flavonoid component corresponding with Rf 0,80; Rf 0,86 and Rf 0,91 can strongly inhibit the acid production of S.mutans GS-5
Pharmaceutical Preparations ; Flavonoids ; Streptococcus mutans

Background: Like other countries in the world, Vietnam has seen the appearance of many infectious diseases such as SARS, influenza A/H1N1. Therefore, monitoring and quick response to infectious diseases, increasingly require enhancing the capacity of test systems. \r\n', u'Objectives: To determine the capacity for microbiological diagnosis and etiology of communicable diseases at the provincial centers for preventive medicine.\r\n', u'Subjects and methods: With the application of the cross-sectional approach, the study was conducted on microbiology diagnostic capacity for communicable diseases surveillance and response system in 55 Provincial Preventive Medicine Centers (PPMCs)\r\n', u'Results: Laboratory testing and confirmation (isolation and bio-chemical tests, gram stain) are only available for common nitrobacteria such as Escherichia coli, Vibrio cholera, Shigella, Salmonella, and some of the respiratory bacteria as streptococcus, meningococcus, etc... in most PPMCs. ELISA/MACELISA technique for detecting virus pathogen such as Arbo viruses (Dengue, Japanese B encephalitis) and Hepatitis B, HIV is also a focus of these PPMCs. However, for diseases caused by other viruses like Polio, Rota, measles, influenza, PPMCs have only the ability to collect specimens. \r\n', u'Conclusions: Most of the cases reported in the surveillance reports are based on clinical signs, only. Case confirmation was done by hospital/regional or national laboratory. \r\n', u'\r\n', u'
microbiological diagnosis ; provincial centers for preventive medicine

Background: Cervical cancer is one of the leading cancers in women worldwide.\r\n', u'Objectives: To evaluate the knowledge, attitude and practice towards cervical cancer and its preventive measures among the parents of young adolescent girls. \r\n', u'Subjects and methods: The cross-sectional survey was conducted in Tuliem District, Hanoi and Cu Chi District, Ho Chi Minh city. By employing a structured interview there have been 218 fathers/mothers of young adolescent girls at secondary school age (11to14 years old) participated in the survey. \r\n', u"Results: There was a limitation in the respondents' understanding about cervical cancer. Although 83% parents have been aware of cervical cancer and 89% considered it as a fatal disease, nearly a half of them does not know any symptoms of the disease. Similarly, inadequate knowledge on causes and risk factors of the disease has been common among respondents. Only 25% of parents are aware of HPV and few knew about the transmission route of this virus. HPV vaccines are still very new for parents. Only 29.8% of interviewees have heard about HPV vaccines. However, 74.3% parents have expressed a wish to have their daughters vaccinated, once HPV vaccines are introduced in Vietnam. \r\n", u'Conclusions: Knowledge, attitude and practice towards cervical cancer among parents of young adolescent girls in these district were quite limited\r\n', u'\r\n', u'
cervical cancer ; knowledge ; attitude ; practice

Background: The strategy of Integrated Management of Childhood Illness (IMCI) was developed as an approach/tool for reducing the childhood mortality in developing countries. IMCI was approved by the Ministry of Health of Viet Nam and has been implemented in more than 3500 communal health centers nationwide. Aims: 1) To discover the quality of health care services for children under-5 and the situation of IMCI in health facilities. 2) To propose solutions to improve the quality of health care services for children. Materials and method: The cross-sectional study using quantitative and qualitative approaches conducted 58 direct observations in health workers, interviewed 58 caretakers, comprehensively interviewed 20 local leaders and technical staffs, facilitated 12 focal group discussions and reviewed 120 records of 12 commune health centers. Results: IMCI was considered by all of the participants as a comprehensive approach to improving the quality of childhood health care at first-level health facilities. IMCI contributed towards improved case management skills of health workers, improved the supply of essential drugs and supplies for child health care. As a result, the quality of health services for children under-5 has been promoted (93% of illness children were correctly assessed and classified, 84.6% of them were correctly treated and counseled). Health workers in Ly Nhan district, Ha Nam province adhered to 8.4+/-1.5 out of 10 essential steps of comprehensive child health care. Contrarily, this indicator was low in the districts of Bac Giang province (4.8+/-1.5), not much different to untrained IMCI health workers. Conclusion: IMCI is a useful strategy to improving the quality of child health care. But there were some difficulties that affected the implementation of this strategy in first-level health facilities.
IMCI ; Child health care

Background: Investment to meet the man power requirements are recognized as urgent; especially to efficiently implement the National Strategy of Preventive Medicine. To strengthen the capability of provincial preventive medicine centers, the Ministry of Health has approved Decision No 05/2006QD-BYT for functions, tasks, authorization and organizational structure of Provincial Preventive Medicine Centers (PPMCs). Objectives: The study was conducted to evaluate the manpower and organization structure of northern PPMCs and provide recommendations for policy makers. Subjects and method: Using the cross-sectional descriptive method, the study covered the preventive medicine centers of 29 northern provinces between Jan to Jun 2007. The information was collected by interviews and self-reported questionnaires. Results:23/29 PPMCs have not met the criteria of man power stated in the Circular 08/2007/TTLB-BYT-BNV. Only 9/29 PPMCs were well organized in accordance with Decision 05/2006/QD-BYT of the Ministry of Health. The average number of staffs in PPMCs was 50+15. Medical staffs accounted for 53%, out of which 21.1% had postgraduate degrees; 32.2% had graduate degrees and 23.3% had been trained in preventive care. Conclusion: To meet the requirements provided by the Decision No05/2006QD-BYT, the man power and training for staffs in PPMCs should be improved and strengthened.
Man power ; Preventive medicine.

Introduction: Antiviral resistance has been reported in seasonal influenza A viruses and avian influenza A(H5N1) viruses in Viet Nam, raising concerns about the efficacy of treatment. Methods: We analysed specimens from two sources during the period 2009–2012: influenza-positive samples from influenza-like illness patients at sentinel clinics in northern Viet Nam and isolates from patients with confirmed A(H5N1) infections. Pyrosequencing was used to detect mutations: H275Y [for A(H1N1) and A(H5N1)], E119V [for A(H3N2)] and I117V [for A(H5N1)]. A neuraminidase inhibition assay was used to determine the Inhibitory Concentration 50 (IC50) values for all influenza A and B isolates. Results: There were 341 influenza A positive samples identified; influenza A(H1N1)pdm09 was identified most frequently (n = 215). In 2009, oseltamivir resistance was observed in 100% (19 of 19) of seasonal A(H1N1) isolates and 1.4% (3/215) of A(H1N1)pdm09 isolates. This H275Y mutation was not found in influenza subtypes A(H5N1) or A(H3N2) isolates. Discussion: In Viet Nam, seasonal and A(H5N1) influenza vaccines are not currently available; thus, effective treatment is required. The presence of oseltamivir-resistant viruses is therefore a concern. Active surveillance for oseltamivir resistance among influenza viruses circulating in Viet Nam should be continued.

Background: In recent years, due to the outbreak of new infectious diseases, re-emerging diseases and bio-terrorist threats, the biological safety for laboratories is essential\r\n', u'Objectives: to evaluate knowledge and practices related to biosafety of researchers in microbiological laboratories\r\n', u'Subjects and method: The study was carried out in the period 2006-2007. Questionnaires and checklists were used for the direct interview the knowledge and observe the practices related to biological safety of 97 laboratory technicians from microbiology laboratories of 22 provincial centers for preventive medicine, which represent for all areas in Vietnam.\r\n', u'Results: The percentage of technicians defines correctly the hazardous groups of some common pathogens are 8.2-33%. The percentage of technicians define correctly the transmission routes of Bacillus anthracis, Staphylococus, Streptococcus are 1%, 15% and 19.6%, respectively. The opinion that thay can wear the laboratory blouse out of laboratories, bring personal belongings into the laboratory and pipeting by mouth are 21.6%, 50.5% and 23.7%, respectively. Regarding laboratory practices: The percentage of technicians does not use gloves is 37.8%; pipeting by mouth: 22.6%. Over 40% technicians do not disinfect working area or washing hands with alcohol after experiments\r\n', u'Conclusion: The results of this study are a basis for planning programs to train, supervise and improve the operational quality of the microbiological laboratory of the provincial preventive health care centers.\r\n', u'\r\n', u'\r\n', u'
Health Knowledge ; Attitudes ; Practice ; Biotechnology/ standards ; Safety/ standards ;

Background: DNA biosensor is based on micro-nano technologies aimed at developing a rapid diagnostic device of infectious diseases and diseases related to genetic change. Biosensors are compact size, high sensitivity and low cost\r\n', u'Objectives: To evaluate effect of biosensor in detecting specific gene fragments of Herpes Simplex virus type 1 and 2 (HSV)\r\n', u'Subjects and method: The electrical signals were recognized by means of transducer and from electrochemical detection of the hybridization between the probe 5\ufffd?AT CAC CGA CCC GGA GAG GGA C-3\ufffd?which were covalently immobilised onto the surface of micro electrodessensors in 3-aminopropyltri-ethoxysilance (APTS)-the conducting polymer matrix and the target (specific DNA sequences of HSV in the sample.\r\n', u'Results: The DNA sensor offers a very high sensitivity, a fast response time, less than 1 min with the DNA target concentration up to 1nM in aqueous media at room temperature.However, in order to detect target DNA in the real samples, samples must be extracted DNA, denatured DNA sequences from a double fiber to single fiber. The measurement should be done soon\r\n', u'Conclusion: The results show a large promise to develop quickly DNA sensors for widely application in bio-medical research \r\n', u'\r\n', u'
Biosensing Techniques ; CNBP protein ; human ;

Background: Hemophilia is the most common clotting disorder in the hereditary blood clotting disorders causing harm to health and psychology. The disease can lead to disability and leave the burden on families and society as well as the development of race\r\n', u"Objectives: To study the familial characteristics of haemophiliacs treated at Regional Hematology and Blood Transfusion Center of Hue Central Hospital. Subject and method: This was a prospective study. It included 48 patients diagnosed and treated Haemophilia A and B at Regional Hematology and Blood Transfusion Center of Hue Central Hospital from 7/2005 - 8/2007. Results: In 48 patients, there were 23 patients who had obviously familial history (included 12 families). They were siblings, cousins, maternal grandfathers or mother's brothers. Among 67 haemophiliacs, 23 haemophiliacs had been studied (34.32%), 30 haemophiliacs died of the disease (44.77%). Most of them died at childhood, below age of 15 years (80.64%). Conclusion: Numbers of deaths in the family was not related to the severity of the disease. The age of clinical detection, morphology, number, site, characteristics of haemorrhage as well as the level of articuar injures were not completely the same between the haemophiliacs of the same family. \r\n", u'\r\n', u'
Hemophilia A/ history ; pathology