1.Detection of Leishmania SP. by PCR method
Journal of Preventive Medicine 2003;13(1):23-25
There is a conservative region of more than 130 bp long in the mitochondria genome of Leishmania mexicana M379 clone E8, and this it is found across many other Leishmania spp. Based on this notion, a pair of PCR primers were designed to amplify the corresponding regions in the minicircles of many other Leishmania species such as L.major, L.donovani. PCR method can be used to detect protozoa in Vietnam
Leishmania
;
Polymerase Chain Reaction
;
diagnosis
2.The use of the recombinant protein HP1125 for detecting helicobacter pylori
Journal of Preventive Medicine 2003;13(4):71-75
The outer membrane protein HP 1125 of Helicobacter pylori was expressed as a recombinant protein in E.Coli by a short time method for Western and ELISA analysis. The analysis was conducted on more than 100 patients in Vietnam
Helicobacter pylori
;
Helicobacter
;
Stomach Diseases
;
diagnosis
;
proteins
3.Supine bicycle of stress echocardiography (SBSE) for detecting coronary artery disease (CAD)
Journal of Practical Medicine 2005;530(11):22-26
The study was conducted in 47 patients (34 males, 13 females) with coronary artery disease or suspected coronary artery disease who were underwent SBSE from May 2002 to October 2004 at 115 People Hospital. Results: CAD was presented in 22 patients: among which, 7 cases with lesion of one major branch, 7 cases with two branches and 8 case with three branches of coronary artery. SBSE test was positive in 20/22 patients, overall sensitivity of 91%, specificity of 80% and accuracy of 85%. The sensitivity was greatest for the font interventricle artery (81%), compared to sensitivity for right coronary artery and the left coronary artery was 56% and 31%, respectively (p<0.005). For identifying patients with multivessels CAD, sensitivity 55%, specificity 97% and accuracy 83%. SBSE is a useful tool for evaluating CAD, and identifying both the location and extent of disease.
Coronary Artery Disease
;
Diagnosis
4.Obstetric ultrasound scanning in urban Vietnam: Is it being over used?
Journal of Practical Medicine 2005;530(11):34-37
The study was conducted at Hanoi Obstetrical and Gynecological Hospital, combining quantitative and qualitative research methods. Results: the study showed that there was a tendency for pregnant women to over-use ultrasound scanning. The reasons for this included women’s perceptions of the usefulness of ultrasound scanning, their serious worries for the fetus, as well as the effects of the market economy on this health service. Over-use of obstetrical ultrasound scanning was not only related to the perception, demands and psychology of pregnant women, but also to socio-economic changes associated with the economic reforms and the liberalization of the health care system.
Ultrasonography
;
Epidemiology
5.Cloning and characterization of A 0.7 KB of a mitochondria minicircle in Leishmania mexicana M379, clone evans 8
Journal of Preventive Medicine 2000;10(3):18-21
A new minicircle in the mitochondria genome of Leishmania mexicana M379, clone E8 was monitored using different methods as PFGE, hybridization,... The 0,7-kb of the circle was cloned and analysed. It appears to have a Chi-like octomer in the region of replication
Mitochondria
;
Leishmania mexicana
6.Cloning and characterization of a mitochondria minicirle in Leishmania mexicana M379, clone evans E8
Journal of Preventive Medicine 2002;12(3):22-24
A mitochondria minicircle in Leishmania mexicana M379, clone evans E8 was cloned and characterized. The molecules exist as dominant class in the mitochondria genome of the parasiters.
Mitochondria
;
Leishmania mexicana
7.Evaluation of polymorphism in resource of genes in medicinal plants of Thai compatriots of Mon Son commune, Con Cuong district, Nghe An Province
Pharmaceutical Journal 2001;305(9):10-12
All medicinal plants of Thai minority at Mon Son commune, Con Cuong District, Nghe An Province. Method: document collection, field test; sample processing. Results: collected 149 species; 129 branches belong 68 families. Parts of use are various: 61 species (41%) use of stem and roots as medicament; 88 species (59%) use of leaves as medicament. The common diseases can be treated are 14 diseases. forest accounts for 64 species (43%), terrace field : 38 species (25%) hill and mountain: 34 species (22%) and rest: others
Polymorphism, Genetic
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Medicine, Traditional
;
Plants, Medicinal
8.Study on the technology to produce inactivated rabies vaccine on the primary hamster kidney cell at laboratory scale
Journal of Preventive Medicine 2005;15(5):123-127
Vnukovo-32 a cell culture rabies is used to study the vaccine strain technology of inactivated culture rabies vaccine production in primary hamster kidney cells. The master and working seeds meet the criteria of rabies vaccine strain titer (5.5 log LD 50 - 7.1 log LD 50/ml) according to WHO standard. During the study, we have used the procedure for inactivated, non concentrated rabies vaccine production of Russia and other countries procedures as references. The result was we constructed the procedure for inactivated, concentrated cell culture rabies vaccine production at laboratory scale.
Rabies Vaccines
;
Cells, Laboratories
9.The tonic effects of "Thap Toan Dai Bo" pills on experimental rats
Journal of Practical Medicine 2004;484(8):6-8
The oral use of "Thap Toan Dai Bo" pills on experimental rats showed a tonic effect with a tonic coefficient of 1.9230.333. 80% of animals had had positive increase of weight. The dose of 0.1ml/10g and 0.2ml/10g, the effect of improving the memory was notified in the time of study. The staying time in light room T (second) and the rate of positive memory in the group of rat using "Thap Toan Dai Bo" was higher than control group with a statistical significant difference. The preparation has not poisonous, without side effect.
Medicine, Traditional
;
Animal Experimentation
;
Rats
10.Serodiagnosis of Japanese Encephalitis (JE) by MAC-ELISA in Bach Mai hospital from 1997-2001
Journal of Medical Research 2003;23(3):21-27
Studying on 1405 sera were collected from 1159 patients ( male 58.55%; female 41.44%) with encephalitis syndromes which were treated in Bach Mai hospital. Using MAC-ELISA to estimate antibody for Japanese Encephalitis in these patients. The results were as following:
The general positive rate was 16.64%
The positive rates by MAC-ELISA were 16.54% (46/278), 10.31% (22/223),...and 13.36% (25/187) in 1997, 1998,...and 2001, respectively.
The positive rates for males were 58.55%; female 41.44%.
The positive rates divided by the age: 1-15 years, 36.84%; 16-60 years 63.16%.
Encephalitis, Japanese
;
Serologic Tests
;
Enzyme-Linked Immunosorbent Assay
;