Poisoned the rats by pesticide wofatox with dose 10mg/kg of their body weight. After 60 minutes, cut off two third of liver by method of Higgins and Anderson. The changes in morphological structures and mitotic activity of liver rats were as follows: Lesions in the liver were markedly manifested, but were not prolong. The mitotic activity of hematocytes decreased. The regenenation of liver weakly manifested
Methyl Parathion ; Rats ; Liver

Study on the changes of ultra and micro-structure of the liver of white rats, which was effected by Wafatox with longed-low dose. Rats with the beginning body mass of 120-150g were everyday oral administrated by oil containing 0.1% of Wafatox with the dose of 0.5mg/kg of their body mass (similarly with 1/70 dose of LD50 for white rats). They were killed by stages of 10, 20, 30, 40 and 50 days from the first oral administration. The results: in clinical, the rat’s intoxicated symptoms were not clear, but the changes in the liver were clearly shown. In the liver, there were some points of necrozism, hepatocytes strongly regressed at the level of ultra and micro-structure. The portals areas and the liver’s tissues were infiltrated by cells like lymphocytes. There were happened stretching veins, stagnating blood and particularly, getting thicker and thicker in the walls of veins of central lobules of the rats’ liver at every stage. Up to the 50th day of intoxication by Wafatox, the quantity of two-nucleus hepatocytes was decreased and mitotic activity of hepatocytes was not increased
Methyl Parathion ; Rats ; Liver

Blood donation for saving the life is a humanitarian and clevated action. The people health is most valuable resource "healthy people - strong country". In Vietnam, there has been not enough of blood for the treatment. The date of blood transfusion safety (7.4) is an opportunity for mobilizing the "blood donation for the life" program.
Blood Donors ; Altruism

Poison the rats by pesticide Bassa with dose 205mg/kg of their body mass. Observe the changes morphological and chemical structure of rats liver, remark: The changes in the liver were clearly show on the thirdly days, find expression: disorder circulation of the blood, degradation cytoplasma of hepatocytes, number hepatocytes with 2 nuclei diminished, decreased in quantities glycogen, increased in the number lipid. After the third day, regeneration of the liver rapidly happened
Poisoning ; Rats ; anatomy & histology ; chemistry ; liver ;

1.000 semen samples of male partner of infertile couples ranging from 20 to 49 year old age were studied. Some parameters such as semen volume, pH, spermatozoa motility, cencentration and morphology were evaluated and leucocyte numbers were counted. Results showed low concentration occurred in the samples of low volume of sperm or of pH < 7.2 (under normal limit). Spermatozoa poor motility was more prequent in the samples of low sperm concentrations of abnormal morphology or of the samples having high density of leucocyte. In the samples of low concentrations of spermatozoa, abnormal morphology of spermatozoa was also frequent.
Semen ; Infertility, Male ; Spermatozoa ; Infertility ; Male

Background: In Vietnam, the proportion infertile couples was about 7-10% of reproductive couples. Assisted reproductive technologies appeared and expanded rapidly. In 1970, sperm preparation techniques were found out, artificial insemination became widespread. Sperm preparation stage is very important in assisted reproductive techniques and increasingly improved. Objectives: To compare the efficiency of two sperm preparation techniques: swim \ufffd?up and discontinuous density gradient. Subjects and method: This was a cross-sectional described study included 30 normal semen samples were chosen based on WHO 1999 criteria. We examined sperm vitality, concentration, motility, morphology before and after applied these techniques. Results: Both procedures resulted in a significant higher percentage of vitality, motility, morphology compared with the original semen sample (p<0.01), but the concentration reduced approximately 5 times from whole semen to sediment when swim \ufffd?up was used and 3 times with density gradient, the difference was statically significant (p< 0.01). Conclusion: Normal semen should be prepared by these method. But in oligozoospermic man, to obtain a higher concentration of sperm, semen should be prepared by density gradient method.
Spermatozoa ; Centrifugation ; Density Gradient ;

Background: Paralysis has been eliminated in Vietnam in 2000. To maintain the achievement, Vietnam needs to have more than 90% of children under 1 age drinking 3 doses of OPV, controlling cases of acute flaccid paralysis and diagnosing for virology in laboratory to determine causes of the disease. Results of laboratory identifying Polio virus and enterovirus in patients with acute flaccid paralysis is evidence of successful in paralytic elimination annually in Vietnam.\r\n', u'Objectives: To of isolate polio virus and enterovirus in patients with acute flaccid paralysis suspected paralysis in 2005.\r\n', u'Subjects and methods: The study included 267 cases with acute flaccid paralysis from 29 provinces, cities in the North and six provinces in the Central of Vietnam during 2005.\r\n', u'Results:Results of surveillance for acute flaccid paralysis and indicators of samples tested in 2005 have reached the criteria regulated by the World Health Organization. 8 poliovirus strains and 56 other non-polio enterovirus strains were isolated from 538 stool specimens of above patients. All isolated poliovirus strains were confirmed as Sabin-like strains. 56 non-polio enterovirus strains included coxsaxkie A (3), conxsackieB (5), echoviruses (34), enterovirus 71 (1) and untyped enteroviruses (13). Wild polio virus was not isolated in 2005.\r\n', u'Conclusion: The result confirmed successfulness in maintaining of poliomyelitis free status in the North and 6 provinces of the Central of Vietnam in 2005.\r\n', u'\r\n', u'\r\n', u'
Paralysis ; Enterovirus ; Poliovirus

Introduction: In Vietnam, the andrology field has been developed in recent years. Some studies have indicated the reasons for male infertility. Amongst them, the most common reason is abnormal semen. Asthenozoospermia rate was found at a higher ratio in abnormal semen analysis. \r\n', u'Objectives: To identify the characteristics of sperm and concentration of FSH, LH and Testosterone in serum; and the association between the sperm morphology and sperm motility as well as the concentration of FSH, LH and Testosterone in serum of asthenozoospermia men. \r\n', u'Subjects and methods: 90 asthenozoospermia male partners of infertile couples. The semen was analyzed at the Laboratory for Tissue and Embryo Preservation, Hanoi Medical University. The hormone concentration was measured by the Automated Chemiluminescence System ACS 180. \r\n', u'Results: The mean semen volume: 3.0 \xb1 1.1 (ml); sperm density: 104.7 \xb1 86.7 million/ml; rapid motility rate: 5.9 \xb1 6.4 (%); sperm vitality rate: 67.4 \xb1 20.1 (%); normal morphology rate: 14.4 \xb1 6.9 (%). In serum, the concentration of FSH was 5.3 \xb1 2.5 lUlL; LH: 4.1 \xb1 1.8 IU/L. Testosterone: 18.3 \xb1 9.4 nmol/L. Conclusions: Among the studied asthenozoospermia men, normal semen volume was found at 91.11 %; normal sperm vitality parameter: 84.4%; normal morphology parameter: 45.56%; normal level of FSH concentration: 47.7%; normal level of LH concentration: 26.66%; normal level of Testosterone concentration: 78.89%. There was a strong association between the sperm morphology and sperm motility as well as serum Testosterone concentration. \r\n', u'
Semen analysis ; Asthenozoospermia ; FSH ; LH ; Testosteron

Background: Idiopathic nephrotic syndrome is not uncommon in children. The disease affects children's physical development. Idiopathic nephrotic syndrome cause many complication in which effecting to physical development has been influenced much to their life, study and living activities of children. Objectives:This study aims to learn about the physical development and to find some factors impacting on physical development ofchildren with idiopathic nephrotic syndrome. Subjects and method: A prospective and descriptive study was conducted on 100 patients diagnosed with idiopathic nephrotic syndrome disease who have been followed up for from 1 year to 6 years in that duration since September 2006 to March 2007. Results:Height standard deviation score (SDS) was less than -2 SO in 15.0% patients. Six per cent of patients had weight SDS ofless than 250. Delayed puberty was seen in 21.6% patients. Some factors impacting on physical developmentwere duration of the disease, relapses, nephrtitis-nephrotic syndrome, prolonged corticoid treatment. Conclusion:Idiopathic nephrotic syndrome retarded physical development and puberty in children. It should be considered careful before indicate to use corticoid because using corticoid for a long period will effect to the height development of children.
Nephrotic Syndrome/ pathology ; therapy ; Idiopathic minimal change nephrotic syndrome ; Child

Background: Bone marrow stem cells with their plasticity can be used to replace and repair the other damaged organs and tissues, so they can also be used to obtain bone healing of nonunions. Objective: to evaluate the results of percutaneous autologous bone marrow grafting to treat the tibia diaphyseal nonunions. Subjects and methods: 12 patients with noninfected nonunion of the tibia were diagnosed and treated in Viet Duc Hospital. About 250mL of marrow was aspirated, then separated and concentrated by density gradient centrifugation. The final mononuclear cell mass containing stem cells and progenitors was washed in 30ml of 0.9% NaCL and then injected into the damaged sites. Patients were evaluated by clinical and X-rays examinations with at least 6 months follow-up. Results: None of the patients had post - op complications. Bone union was obtained in eleven of the twelve patients (91,7%) at an average of 15,3 weeks (range, 9 - 30 weeks), the bone marrow grafts used for these patients who had bone union contained a mean of 5,65 \xb1 3,74 x 106 (0,95 - 11,73 x 106) CD34(+) stem cells in total. Conclusions: Percutaneous autologous bone - marrow grafting is a minimally invasive alternative and a simple, effective, safe method for the treatment of the tibia diaphyseal nonunions with the comparative bone healing rate. \r\n', u'\r\n', u'
Tibia/ pathology ; Bone Marrow/ anatomy & ; histology ; surgery