Hopea Odorata Roxb (HO) is often used by many Vietnamese people as a remedy for dental caries. However, nothing is known about which compound(s) of the material and how it (they) acts to protect the teeth. It was found in this study that the ethanol extract of Hopea odorata Roxb bark (HOE) was strongly inhibited acid production of Streptococcus mutans cells in suspension and biofilms as well. The extract also killed the organisms in acidified medium. At a concentration of 10% HOE, 90% of population was killed by 10 min and 30 min at pH 4.0 and 7.0, respectively. Killing ability of HOE was augmented when combined with hydroperoxide. In reduced pH and killing assays, the cells in biofilm appeared to be more resistant to the effect of the HOE compared to the cells in suspension. Further investigation should be needed to elucidate the anticariogenic effects of HOE
Medicine, Traditional ; Pharmaceutical Preparations

Background: The method of immunoelectron microscopy has been found more than 20 years. It is widely applied to detect and identify some types of virus in medical waste samples.\r\n', u'Objectives: To identify antigen location of Rota virus in organelle of the Vero cell and primary monkey kidney cells after infecting and to study the interaction between the virus and host cells.\r\n', u'Subjects and methods: The study was conducted on Rota virus G1P8 (KH0118) isolated from patients with symptoms of acute diarrhea, primary monkey kidney cells collected from Macaca mulatta monkey and the Vero cell of WHO. \r\n', u'Results: Gold particles (10nm) coated protein A and polyclonal antibodies were used to interact directly with Rotavirus proteins \r\n', u'These gold particles with high electron density revealed the antigen location of the Rota virus in the lysosome, pouch and other compartments of the cytoplasm.\r\n', u'Newly assembled viral particles could be identified only after 18-20hours post-infection. It is also noteworthy that viral particles and empty capsides (virus like particles) were comprised into cytoplasmic vesicles associated with the endoplasmic reticulum (ER)-Golgi system.\r\n', u'Conclusion: In order to better understand the interaction mechanism of virus and host cells, the use of this method together with specific monoclonal antibodies for each protein component of viruses and cells is essential.\r\n', u'\r\n', u'\r\n', u'
Rotavirus ; Vero Cells ;

Background: DNA biosensor is based on micro-nano technologies aimed at developing a rapid diagnostic device of infectious diseases and diseases related to genetic change. Biosensors are compact size, high sensitivity and low cost\r\n', u'Objectives: To evaluate effect of biosensor in detecting specific gene fragments of Herpes Simplex virus type 1 and 2 (HSV)\r\n', u'Subjects and method: The electrical signals were recognized by means of transducer and from electrochemical detection of the hybridization between the probe 5\ufffd?AT CAC CGA CCC GGA GAG GGA C-3\ufffd?which were covalently immobilised onto the surface of micro electrodessensors in 3-aminopropyltri-ethoxysilance (APTS)-the conducting polymer matrix and the target (specific DNA sequences of HSV in the sample.\r\n', u'Results: The DNA sensor offers a very high sensitivity, a fast response time, less than 1 min with the DNA target concentration up to 1nM in aqueous media at room temperature.However, in order to detect target DNA in the real samples, samples must be extracted DNA, denatured DNA sequences from a double fiber to single fiber. The measurement should be done soon\r\n', u'Conclusion: The results show a large promise to develop quickly DNA sensors for widely application in bio-medical research \r\n', u'\r\n', u'
Biosensing Techniques ; CNBP protein ; human ;

Background
  The Millennium Development Goals were put forth to strengthen maternal and child health, and because infectious disease has become a trend in the world, ensuring the quality and quantity of nursing is important. We report on a survey detailing the current status of legal frameworks and qualification systems in nursing in four countries: Cambodia, Lao People’s Democratic Republic (PDR), Myanmar, and Vietnam.
Method
  We conducted a semi-structured interview with nursing administrative officers, and then we confirmed those information in their countries in 2012 and 2013. We categorized the existing nursing legal framework, nursing qualification systems, professional association and council.
Results
  The King of Cambodia stipulated to a royal decree, which was established by the nursing council. In Lao PDR, following the Law on Health Care, two nursing and midwifery related regulations were developed. The Union of Myanmar enacted the Nurse and Midwife Law. In Vietnam following the Law on Medical Examination and Treatment, two Circulars were issued by the Ministry of Health to regulate the scope of practices and norms. We report on the current situation of the qualification and registration systems for nursing of each country. In addition, the development of qualifications and regulations should be reported based on the ASEAN Mutual Recognition Agreement (MRA).
Conclusion
  We conducted a comparison of four countries to ensure the quality of nursing. A challenge for the future is compliance and dissemination of the laws and regulations that have been developed. ASEAN MRA has become a driving force for improvement of these legal systems.

Four Trichoderma strains (CH2, SH16, PQ34, and TN42) were isolated from soil samples collected from Quang Tri and Thua Thien Hue provinces in Vietnam. The strains exhibited high chitinolytic secretion. Strain PQ34 formed the largest zone of chitinase-mediated clearance (> 4 cm in diameter) in agar containing 1% (w/v) colloidal chitin. Analysis of the internal transcribed spacer regions of these strains indicated that they were Trichoderma asperellum. The molecular weights of the chitinases were approximately 42 kDa. Chitinase genes (chi42) of T. asperellum strains TN42, CH2, SH16, and PQ34 were 98~99% homologous to the ech42 gene of T. harzianum CB-Pin-01 (accession No. DQ166036). The deduced amino acid sequences of both T. asperellum strains SH16 and TN42 shared 100% similarity.
Agar ; Amino Acid Sequence ; Chitin ; Chitinase ; Colloids ; Molecular Weight ; Soil ; Sprains and Strains ; Trichoderma ; Vietnam

In this study, we report the manganese peroxidase production ability from a Fusarium sp. strain using an inexpensive medium of agriculture residues of either rice straw or wood chips as carbon source. The highest manganese peroxidase activity on rice straw medium and on wood chips was 1.76 U/mL by day 9 and 1.91 U/mL by day 12, respectively.
Agriculture ; Carbon ; Fusarium* ; Manganese* ; Mass Screening* ; Peroxidase* ; Wood

Ultrasonic computed tomography based on back scattering theory is the most powerful and accurate tool in ultrasound based imaging approaches because it is capable of providing quantitative information about the imaged target and detects very small targets. The duple-frequency distorted Born iterative method (DF–DBIM), which uses density information along with sound contrast for imaging, is a promising approach for imaging targets at the level of biological tissues. With two frequencies f₁ (low) and f₂ (high) through Nf₁ and Nf₂ iterations respectively, this method is used to estimate target density along with sound contrast. The implications of duple-frequency fusion for the image reconstruction quality of density information along with sound contrast based ultrasound tomography have been analyzed in this paper. In this paper, we concentrate on the selection of parameters that is supposed to be the best to improve the reconstruction quality of ultrasound tomography. When there are restraints imposed on simulated scenarios to have control of the computational cost, the iteration number Nf₁ is determined resulting in giving the best performance. The DF–DBIM is only effective if there are a moderate number of iterations, transmitters and receivers. In case that the number of transducers is either too large or too small, a result of reconstruction which is better than that of the single frequency approach is not produced by the implementation of DF–DBIM. A fixed sum N(iter) of Nf₁ and Nf₂ was given, the investigation of simulation results shows that the best value of Nf₁ is [N(iter)/2 − 1]. The error, when applying this way of choosing the parameters, will be normalized with the reduction of 56.11%, compared to use single frequency as used in the conventional DBIM method. The target density along with sound contrast is used to image targets in this paper. It is a fact that low-frequency offers fine convergence, and high-frequency offers fine spatial resolution. Wherefore, this technique can effectively expand DBIM's applicability to the problem of biological tissue reconstruction. Thanks to the usage of empirical data, this work will be further developed prior to its application in reality.
Image Processing, Computer-Assisted ; Methods ; Transducers ; Ultrasonics ; Ultrasonography

BACKGROUND: Polycystic ovarian syndrome (PCOS) is one of the most common endocrinopathies among reproductive-age women. Its metabolic features often overlap with those associated with metabolic syndrome (MS) and insulin resistance syndrome (IRS). The objective of this study was to determine the prevalence and predictors of MS and IRS in infertile Vietnamese women with PCOS. METHODS: A cross-sectional study was conducted at a tertiary fertility centre at Hue University Hospital from June 2016 to November 2017. A total of 441 infertile women diagnosed with PCOS based on the revised 2003 Rotterdam consensus criteria were enrolled. MS and IRS were defined based on the National Heart, Lung, and Blood Institute/American Heart Association Adult Treatment Panel III 2005 and American College of Endocrinology IRS 2003 criteria, respectively. Complete clinical and biochemical measurements of 318 women were available for analysis. Independent predictors of MS and IRS were identified using multivariate logistic regression. RESULTS: The overall prevalence of MS and IRS in women with PCOS was 10.4% and 27.0%, respectively. We identified older age (>30 years) and obesity as independent predictors of MS and IRS. Elevated anti-Müllerian hormone levels increased the risk of IRS, but not that of MS. CONCLUSION: MS and IRS are prevalent disorders among infertile Vietnamese women with PCOS. PCOS is not solely a reproductive problem. Screening and early intervention for MS and/or IRS based on anthropometric, metabolic, and reproductive hormone risk factors should be an integral part of fertility care.
Adult ; Asian Continental Ancestry Group ; Consensus ; Cross-Sectional Studies* ; Early Intervention (Education) ; Endocrinology ; Female ; Fertility ; Heart ; Humans ; Infertility ; Insulin Resistance* ; Insulin* ; Logistic Models ; Lung ; Mass Screening ; Obesity ; Polycystic Ovary Syndrome* ; Prevalence ; Risk Factors ; Vietnam*

Globally, providing evidence on the economic burden of chronic obstructive pulmonary disease (COPD) is becoming essential as it assists the health authorities to efficiently allocate resources. This study aimed to summarize the literature on economic burden evidence for COPD from 1990 to 2019. This study examined the economic burden of COPD through a systematic review of studies from 1990 to 2019. A search was done in online databases, including Web of Science, PubMed/Medline, Scopus, and the Cochrane Library. After screening 12,734 studies, 43 articles that met the inclusion criteria were identified. General study information and data on direct, indirect, and intangible costs were extracted and converted to 2018 international dollars (Int$). Findings revealed that the total direct costs ranged from Int$ 52.08 (India) to Int$ 13,776.33 (Canada) across 16 studies, with drug costs rannging from Int$ 70.07 (Vietnam) to Int$ 8,706.9 (China) in 11 studies. Eight studies explored indirect costs, while one highlighted caregivers’ direct costs at approximately Int$ 1,207.8 (Greece). This study underscores the limited research on COPD caregivers’ economic burdens, particularly in developing countries, emphasizing the importance of increased research support, particularly in high-resource settings. This study provides information about the demographics and economic burden of COPD from 1990 to 2019. More strategies to reduce the frequency of hospital admissions and acute care services should be implemented to improve the quality of COPD patients’ lives and reduce the disease’s rising economic burden.

This article describes Viet Nam Ministry of Health’s (VMoH) activities to prepare for and respond to the threat Zika virus (ZIKV), including the adaptation of existing surveillance systems to encompass ZIKV surveillance.