1-AT is a potent inhibitor of protease in human serum. 1-AT was successfully purified by a 5 step procedure: (1) 34%-80% ammonium sulfate precipitation, (2) affinity chromatography on Cibacron blue sepharose column, (3) ion-exchange chromatography on DEAE-sephadex A50, (4) 80% ammonium sulfate precipitation and HPLC. with the arginin-HCL 10% buffer, pH 6.0; (3) ion-exchange on DEAE-sephadex A50 and (5) HPLC. A molecular weight and isoelectropoint of the albumin are similar with those of the 1-AT. That is why, affinity chromatography on cibacron blue sepharose column to remove the serum albumin is a very important step of the 1-AT purification. The purified 1-AT is in 91-fold purification with recovery of 32.1% specific inhibitory activity of 32978.4 per gam protein
Plasma ; alpha 1-Antitrypsin