No abstract available.
Neuropeptide Y* ; Neuropeptides*

OBJECTIVETo observe effects of acupuncture on BMI, WHR and serum levels of leptin and neuropeptide Y in simple obesity Uigur patients to research the mechanism of acupuncture in slimming.

METHODSThirty simple obesity Uigur patients were treated with body acupuncture combined with electroacupuncture. Two courses later, their therapeutic effects were observed. And the above indexes were determined before and after treatment.

RESULTSThe clinical total effective rate was 80.00%, and BMI, WHR, and the serum leptin level decreased (P < 0. 001).

CONCLUSIONUigur patients of simple obesity possibly exist resistance of leptin and acupuncture can decrease the peripheral leptin content.

Acupuncture Therapy ; Electroacupuncture ; Humans ; Leptin ; blood ; Neuropeptide Y ; Obesity ; therapy

The distribution and morphology of cholecystokinin-8 (CCK 8)-, neuropeptide Y (NPY)-, Substance P (SP)- and vasoactive intestinal polypeptide (VIP)-immunoreactivity were examined in the cat superior colliculus (SC) by means of immunohistochemistry. Judging from this study, some neuropeptides-IR neurons may be present in the superficial layers of the sc. And these neurons and neuropeptides may be involved in the functions of the superficial layers.
Animals ; Brain* ; Cats* ; Immunohistochemistry ; Neurons* ; Neuropeptide Y ; Neuropeptides ; Substance P ; Superior Colliculi* ; Vasoactive Intestinal Peptide

OBJECTIVETo explore the effect of negative emotions on serum levels of adrenocorticotropic hormone (ACTH) and neuropeptide Y (NYP) in hepatitis B liver cirrhosis (HBLC) patients.

METHODSTotally 617 HBLC patients were assigned to the negative emotion group (415 cases) and the non-negative emotion group (202 cases) judged by negative emotions. Case numbers of various grading Child-Pugh were recorded in the two groups. Their liver functions were compared between the two groups. Serum levels of ACTH and NPY were detected using double antibody sandwich enzyme-linked immunosorbent assay (ELISA) in the two groups.

RESULTSThere was no statistical difference in Child-Pugh grading between the two groups (χ2 = 0.65, P = 0.72). Compared with the non-negative emotional group, serum ACTH levels decreased significantly in the negative emotion group with statistical difference (P < 0.05). There was no statistical difference in serum ACTH levels between the two groups (P > 0.05).

CONCLUSIONThe negative emotion of HBLC patients was not related to the serum ACTH level, but to relatively lower-concentration serum NPY levels.

Adrenocorticotropic Hormone ; blood ; Emotions ; Hepatitis B ; blood ; psychology ; Humans ; Liver Cirrhosis ; blood ; psychology ; Neuropeptide Y ; Serum

OBJECTIVETo search for a method for increasing therapeutic effect on hypertension and study on the mechanism.

METHODSSeventy-five cases were randomly divided into the treatment group (n=45) treated by acupuncture plus medicine, and the control group (n=30) treated by medicine. Their blood pressure and plasma neuropeptide Y (NPY) before and after treatment were investigated.

RESULTSBlood pressure and NPY content in both the two groups decreased significantly (P < 0.01), and the treatment group in decreasing blood pressure and NPY content was superior to the control group (P < 0.05).

CONCLUSIONAcupuncture and medicine have cooperation in treatment of hypertension, which is performed possibly through decreasing NPY.

Acupuncture Therapy ; Blood Pressure ; Blood Pressure Determination ; Humans ; Hypertension ; Neuropeptide Y ; blood

No abstract available.
Aging ; Animals ; Cerebral Cortex* ; Neurons* ; Neuropeptide Y* ; Neuropeptides* ; Nitric Oxide Synthase* ; Nitric Oxide* ; Rats*

To study the expression and distribution of neuropeptide Y (NPY) and long leptin receptor (OB-Rb) in the gastrointestinal tract of giant panda, samples of three animals were collected from the key laboratory for reproduction and conservation genetics of endangered wildlife of Sichuan province, China conservation and research center for the giant panda. Paraffin sections of giant panda gastrointestinal tissue samples were observed using hematoxylin-eosin staining (HE) and strept actividin-biotin complex immunohistochemical staining (IHC). The results show that the intestinal histology of three pandas was normal and no pathological changes, and there were rich single-cell and multi-cell mucous glands, long intestinal villi and thick muscularis mucosa and muscle layer. Positive cells expressing NPY and OB-Rb were widely detected in the gastrointestinal tract by IHC methods. NPY positive nerve fibers and neuronal cell were widely distributed in submucosal plexus and myenteric plexus, especially in the former. They were arranged beaded or point-like shape. NPY positive cells were observed in the shape of ellipse and polygon and mainly located in the mucous layer and intestinal glands. OB-Rb positive cells were mainly distributed in the mucous layer and the laminae propria, especially the latter. These results confirmed that NPY and OB-Rb are widely distributed in the gut of the giant panda, which provide strong reference for the research between growth and development, digestion and absorption, and immune function.
Animals ; China ; Intestines ; metabolism ; Neuropeptide Y ; genetics ; metabolism ; Receptors, Leptin ; genetics ; metabolism ; Ursidae ; genetics ; metabolism

Animals ; Chickens ; physiology ; Eating ; drug effects ; Injections, Intraventricular ; methods ; Neuropeptide Y ; administration & dosage ; pharmacology

A detailed study of the distribution of neuropeptide Y(NPY) in the olfactory bulb of the male adult and aging rat brains was undertaken by means of immunohistochemistry. Adult rats(Sprague-Dawley, 12-14 weeks old) were used in the control group. And aging rats(30 months old) were used in the experimental group. Brain tissues of both rat groups were obtained from each animal which were perfused transcardially with 0.9% NaCl followed by a 4% paraformaldehyde solution. Coronal serial sections(30nm) were cut on a freezing microtome, and were immunostained with the rabbit-raised antiserum to neuropeptide Y. In the control group, neuropeptide Y-immunoreactive(NPY-IR) neurons were mainly found in the white matter and deep internal granule cell layer, and rarely in the external plexiform layer of main olfactory bulb. In case of accessory olfactory bulb, a few of NPY-IR neurons were found in the internal granule cell layer. Virtually most neurons were medium-sized(10-20nm), and appeared to be bipolar or bitufted in shape. And small numbers of large-sized multipolar neurons(20-30nm) were found in the internal granule cell layer of main olfactory bulb. In the experimental aging rats, the numbers of NPY-IR neurons were decreased, that is to say, NPY-IR neurons in aging group were found average 2.5 neurons per each section compared with average 4.5 per each section in the control group. Also many perikarya of NPY-IR neurons appeared shrinkage, oval or round in shape. In brief, NPY-IR neurons of the olfactory bulb seem to be decreased in number of the neurons in aging rat. These findings may concern with reduction of olfactory function.
Adult* ; Aging* ; Animals ; Brain ; Freezing ; Humans ; Immunohistochemistry ; Male ; Neurons* ; Neuropeptide Y ; Neuropeptides* ; Olfactory Bulb* ; Rats*

The postnatal development of somatostatin [SOM]- and neuropeptide Y[NPY]- immunoreactive[ir] neurons were examined in rat cerebral cortex considering their coexistence in cortical neurons. Using double immunohistochemical staining for SOM and NPY with diaminobenzidine and benzidine dihydrochloride as chromogens, we subdivided immunoreactive cells into double-labeled SOM/NPY-, SOM only-, and NPY only-ir neurons. Interestingly, SOM/NPY- and SOM only-ir neurons were detectable even at the day of birth, in contrast to NPY only-ir cells which first appeared in most cortices from two weeks of age. The morphological features of double-labeled SOM/NPY neurons were not identical to those SOM only- and NPY only-ir neurons. No apparent changes in the shape and size of single-labeled neurone occurred with age ; throughout their postnatal life they were round and ovoid, had a thin rim of perinuclear cytoplasm, and short processes. In contrast, the features of SOM/NPY-ir neurons were not consistent during postnatal life. By day P7, these neurons showed immature features ; they began to show more advanced neuronal characteristics by week P2, when they had a larger and more intensely-stained cytoplasm. In addition, their processes were longer, thicker and more complex than at earlier ages. At this age, SOM/NPY-ir somata were close to their maximum size. From week P4, they became smaller and were lightly labeled. SOM/NPY-ir somata were larger than SOM only- and NPY only-ir somata at and after two weeks of age. The present results showing different postnatal maturation patterns such as time of appearance and morphological features suggest that double-labeled SOM/NPY and single-labeled neurons might be different populations regulated by different mechanisms in their development, and with different functional properties during development.
Animals ; Cerebral Cortex* ; Cytoplasm ; Neurons* ; Neuropeptide Y* ; Neuropeptides* ; Parturition ; Rats* ; Somatostatin*