To construct a sensory neuropathy model, excess pyridoxine (150 mg/kg s.i.d.) was injected subcutaneously in dogs over a period of 7 days. During the administrations period, the dogs experienced body weight reduction and proprioceptive loss involving the hindquarters. After pyridoxine administration was completed, electrophysiological recordings showed that the M wave remained at a normal state, but the H-reflex of the treated dogs disappeared at 7 days. The dorsal funiculus of L4 was disrupted irregularly in the axons and myelin with vacuolation. The dorsal root ganglia of L4, and sciatic and tibial nerves showed degenerative changes and vacuolation. However, the lateral and ventral funiculi of L4 showed a normal histopathologic pattern. Although this subcutaneous administration method did not cause systemic toxicity and effectively induced sensory neuropathy, this study confirmed the possibility of producing a pyridoxine-induced sensory neuropathy model in dogs with short-term administration.
Animals ; Body Weight/drug effects ; *Disease Models, Animal ; Dogs ; Electrophysiology ; Neurodegenerative Diseases/*chemically induced/pathology ; Proprioception/drug effects ; Pyridoxine/*toxicity

OBJECTIVETo investigate the effect of the new Traditional Chinese Compound Shenwu Capsule on the damage of lymphocyte DNA and lipid peroxidation in peripheral blood of rats induced by beta-amyloid injection.

METHODThe animal model was made by injection of beta-amyloid25-35 into hippocampus of rats. DNA damage of lymphocytes was measured by the single cell gel electrophoresis (SCGE) combined with the laser scanning confocal microscopy (LSCM). The content of malondialdehyde (MDA) was determined by TBA assay.

RESULTShenwu Capsule decreased the rate of the comet-like cell, comet-like cell lengh (TCL), and tail moment (TM) of lymphocytes in peripheral blood of model rats. Shenwu Capsule also declined the MDA content in serum of Abeta model rats.

CONCLUSIONShenwu Capsule has protective effect on peripheral blood lymphocyte DNA.

Amyloid beta-Peptides ; Animals ; Capsules ; DNA Damage ; drug effects ; Drug Combinations ; Drugs, Chinese Herbal ; administration & dosage ; isolation & purification ; pharmacology ; Lymphocytes ; drug effects ; Male ; Neurodegenerative Diseases ; chemically induced ; pathology ; Neuroprotective Agents ; administration & dosage ; pharmacology ; Panax ; chemistry ; Plants, Medicinal ; chemistry ; Polygonum ; chemistry ; Pueraria ; chemistry ; Rats ; Rats, Sprague-Dawley