1.Recent advances in molecular genetics of spinocerebellar ataxia type 3/Machado-Joseph disease.
Dandan JIA ; Hong JIANG ; Beisha TANG
Chinese Journal of Medical Genetics 2008;25(6):660-662
To date, nearly 28 distinct genetic loci of autosomal dominant cerebellar ataxias have been identified, among them 18 disease-causing genes have been cloned. Of these, Machado-Joseph disease (MJD), also named as spinocerebellar ataxia type 3 (SCA3), is perhaps the most common subtype among different races and origins in the world. It is a neurodegenerative disease caused by the expansion of a CAG repeat in the coding region of the MJD1 gene, with obvious clinical and genetic heterogeneity. In this review, authors covered the recent advances in molecular genetic of SCA3/MJD.
Ataxin-3
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Humans
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Machado-Joseph Disease
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genetics
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Molecular Biology
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Mutation
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Nerve Tissue Proteins
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chemistry
;
genetics
;
metabolism
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Nuclear Proteins
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chemistry
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genetics
;
metabolism
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Repressor Proteins
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chemistry
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genetics
;
metabolism
2.Properties of GST-CALM expressed in E. coli.
Jeong Ah KIM ; Seong Ryul KIM ; Yong Keun JUNG ; So Youn WOO ; Ju Young SEOH ; Young Sook HONG ; Hyung Lae KIM
Experimental & Molecular Medicine 2000;32(2):93-99
Clathrin-coated vesicles (CCVs) are involved in protein and lipid trafficking between intracellular compartments in eukaryotic cells. CCVs are composed of clathrin and assembly proteins. The clathrin assembly protein lymphoid myeloid leukemia (CALM) gene, encodes a homologoue of the neuronal clathrin assembly protein AP180. In this study, we characterized the properties of the CALM expressed in E. coli. The molecular weight of bacterially expressed GST-CALM fusion protein was approximately 105 kD on SDS-PAGE. The CALM protein could promote clathrin triskelia into clathrin cages and could bind the preformed clathrin cage. However, 33 kD N-terminal domain of CALM could not bind pre-assembled clathrin cages, but assemble clathrin triskelia into clathrin cages. The CALM protein was bound to SH3 domain through N-terminal domain1, in vitro. The CALM protein is proteolyzed by caspase 3, caspase 8 and calpain through C-terminal domain.
Animal
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Antibodies, Monoclonal
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Calpain/chemistry
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Caspases/chemistry
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Clathrin-Coated Vesicles/metabolism*
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Electrophoresis, Polyacrylamide Gel
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Escherichia coli/metabolism
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Escherichia coli/genetics
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Female
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Glutathione Transferase/genetics*
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Mice
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Mice, Inbred BALB C
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Nerve Tissue Proteins/metabolism
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Nerve Tissue Proteins/metabolism
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Nerve Tissue Proteins/chemistry*
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Phosphoproteins/metabolism
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Phosphoproteins/genetics
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Phosphoproteins/chemistry*
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Protein Binding
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Rabbits
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Recombinant Fusion Proteins/metabolism
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Recombinant Fusion Proteins/genetics
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Recombinant Fusion Proteins/chemistry*
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src Homology Domains
3.Overexpression of response gene to complement-32 promotes cytoskeleton reorganization in SW480 cell line.
Jie TIAN ; Chuan XU ; Min-hui YANG ; Zu-guo LI
Journal of Southern Medical University 2011;31(7):1179-1182
OBJECTIVETo construct the recombinant plasmid pcDNA3.0-RGC32 and evaluate the effect of the response gene to complement-32 (RGC32) on cell cytoskeleton in vitro.
METHODSThe full-length cDNA of RGC32 was obtained by RT-PCR and inserted into the eukaryotic expression vector pcDNA3.0 to generate the recombinant plasmid pcDNA3.0-RGC32. After transfection of the recombinant plasmid into SW480 cells, the expression of RGC32 in the cells was detected by Western blotting. The cytoskeleton of SW480 cells was visualized before and after the transfection, and the changes in the cell migration ability was assessed by wound-healing assay.
RESULTSThe recombinant plasmid pcDNA3.0-RGC32 was successfully constructed. The expression of RGC32 was significantly increased in SW480 cells after transfection with pcDNA3.0-RGC32. Before the transfection, the microfilaments of SW480 cells were few and short without obvious polarity, but after the transfection, the microfilaments were increased and elongated with also an obvious polarity, and the invasive structures of lamellae and lamellipodia occurred. The migration ability of the cells was enhanced after transfection with pcDNA3.0-RGC32.
CONCLUSIONOverexpression of RGC32 can cause the reorganization of cytoskeleton and promotes the cell migration, which can be an important mechanism of RGC32 in promoting cancer metastasis.
Cell Cycle Proteins ; biosynthesis ; genetics ; Cell Line, Tumor ; Cell Movement ; Colorectal Neoplasms ; genetics ; metabolism ; pathology ; Cytoskeleton ; chemistry ; metabolism ; Genetic Vectors ; Humans ; Muscle Proteins ; biosynthesis ; genetics ; Neoplasm Metastasis ; genetics ; Nerve Tissue Proteins ; biosynthesis ; genetics ; Plasmids ; genetics ; Recombinant Proteins ; biosynthesis ; genetics
4.Neuroglobin and hypoxic-ischemic brain bamage.
Li ZHANG ; Li-Hua LI ; Yi QU ; De-Zhi MU
Chinese Journal of Contemporary Pediatrics 2008;10(2):265-268
5.Expression pattern of polycomb gene Nspc1 at the early developmental stage in zebrafish.
Xu-Dong WU ; Min ZHANG ; Yan-Hua GONG ; Bo-Qin QIANG ; Jian-Gang YUAN ; An-Ming MENG ; Xiao-Zhong PENG
Acta Academiae Medicinae Sinicae 2008;30(5):550-553
OBJECTIVETo study the expression pattern of Polycomb gene Nspc1 at the early developmental stage in zebrafish.
METHODSIn situ hybridization probe for Nspc1 was designed according to the GenBank information. Collecting zebrafish embryos at different stages including one cell stage, two-cell stage, bud stage, and somites stage, we hybridized them with the prepared probe. Then the hybridization signals at different intervals were observed and photographed at the right time.
RESULTSNspc1 was expressed globally at the early stage. Its expression specificity began at the somites stage, mainly in the nervous system of the head.
CONCLUSIONNspc1 may play essential roles in the early stage development of zebrafish, especially in the nervous system.
Amino Acid Sequence ; Animals ; Gene Expression Regulation, Developmental ; Humans ; Mice ; Molecular Sequence Data ; Nerve Tissue ; growth & development ; metabolism ; Polycomb Repressive Complex 1 ; Repressor Proteins ; chemistry ; genetics ; metabolism ; Sequence Alignment ; Zebrafish ; genetics ; growth & development ; metabolism ; Zebrafish Proteins ; chemistry ; genetics ; metabolism
6.Time course of expression of intermediate filament protein vimentin, nestin and desmin in rat renal glomerular injury.
Jun ZOU ; Tian-hui CHANG ; He CHANG ; Eishin YAOITA ; Yutaka YOSHIDA ; Masaaki NAMETA ; Tadashi YAMAMOTO ; Xin JIN
Chinese Medical Journal 2007;120(13):1203-1205
Animals
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Desmin
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analysis
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genetics
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Female
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Immunohistochemistry
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Intermediate Filament Proteins
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analysis
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genetics
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Kidney Glomerulus
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chemistry
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Nephrosis
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metabolism
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Nerve Tissue Proteins
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analysis
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genetics
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Nestin
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Podocytes
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chemistry
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RNA, Messenger
;
analysis
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Rats
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Rats, Inbred WKY
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Vimentin
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analysis
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genetics
7.Expression of nesfatin-1/NUCB2 and ghrelin in gastric mucosa of rats with intrauterine growth retardation.
Ya-Ying CHENG ; Hong-Yan LV ; Xin WANG ; Guang-Yao SONG
Chinese Journal of Contemporary Pediatrics 2014;16(10):1051-1056
OBJECTIVETo investigate the expression of nesfatin-1/NUCB2 and ghrelin in the gastric mucosa of rats with intrauterine growth retardation (IUGR) and its significance.
METHODSThe IUGR animal model was established by feeding rats low-protein diets during their pregnancy. Newborn rats were divided into catch-up growth, non-catch-up growth and control groups. Protein and mRNA levels of nesfatin-1/NUCB2 and ghrelin in the gastric mucosa of rats were determined by RT-PCR and Western blot, respectively.
RESULTSNesfatin-1/NUCB2 mRNA and protein were expressed in the gastric mucosa of rats immediately after birth, and their expression increased in an age-dependent manner in all three groups. Furthermore, the level of nesfatin-1/NUCB2 in the catch-up growth group was higher than that in the control group before weaning, whereas there was no significant difference in nesfatin-1/NUCB2 expression between the two groups after weaning. The level of nesfatin-1/NUCB2 in the non-catch-up growth group was lower than that in the catch-up growth group during the whole observation period. The level of ghrelin in the catch-up growth group was higher than that in the control group starting from day 12 after birth, whereas there was no significant difference in ghrelin expression between the two groups after weaning. The level of ghrelin in the non-catch-up growth group was lower compared with those in the catch-up growth and control groups from days 12 to 28 after birth.
CONCLUSIONSNesfatin-1 and ghrelin are co-expressed in the gastric mucosa of rats with IUGR after birth and interact with each other to produce long-term nutritional regulation.
Age Factors ; Animals ; Calcium-Binding Proteins ; analysis ; genetics ; DNA-Binding Proteins ; analysis ; genetics ; Female ; Fetal Growth Retardation ; metabolism ; Gastric Mucosa ; chemistry ; Ghrelin ; analysis ; genetics ; Male ; Nerve Tissue Proteins ; analysis ; genetics ; RNA, Messenger ; analysis ; Rats ; Rats, Sprague-Dawley
8.Gingerol activates noxious cold ion channel TRPA1 in gastrointestinal tract.
Meng-Qi YANG ; Lin-Lan YE ; Xiao-Ling LIU ; Xiao-Ming QI ; Jia-Di LV ; Gang WANG ; Ulah-Khan FARHAN ; Nawaz WAQAS ; Ding-Ding CHEN ; Lei HAN ; Xiao-Hui ZHOU
Chinese Journal of Natural Medicines (English Ed.) 2016;14(6):434-440
TRPA1 channels are non-selective cation channels that could be activated by plant-derived pungent products, including gingerol, a main active constituent of ginger. Ginger could improve the digestive function; however whether ginger improves the digestive function through activating TRPA1 receptor in gastrointestinal tract has not been investigated. In the present study, gingerol was used to stimulate cell lines (RIN14B or STC-1) while depletion of extracellular calcium. TRPA1 inhibitor (rethenium red) and TRPA1 gene silencing via TRPA1-specific siRNA were also used for mechanistic studies. The intracellular calcium and secretion of serotonin or cholecystokinin were measured by fura-2/AM and ELISA. Stimulation of those cells with gingerol increased intracellular calcium levels and the serotonin or cholecystokinin secretion. The gingerol-induced intracellular calcium increase and secretion (serotonin or cholecystokinin) release were completely blocked by ruthenium red, EGTA, and TRPA1-specific siRNA. In summary, our results suggested that gingerol derived from ginger might improve the digestive function through secretion releasing from endocrine cells of the gut by inducing TRPA1-mediated calcium influx.
Calcium
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metabolism
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Calcium Channels
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genetics
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metabolism
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Catechols
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pharmacology
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Cell Line
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Fatty Alcohols
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pharmacology
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Gastrointestinal Tract
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drug effects
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metabolism
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Ginger
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chemistry
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Humans
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Nerve Tissue Proteins
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genetics
;
metabolism
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Plant Extracts
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pharmacology
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TRPA1 Cation Channel
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Transient Receptor Potential Channels
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genetics
;
metabolism
9.Comparative proteomic analysis of cerebral cortex between middle cerebral artery occlusion rats and normal controls.
Xiao-Feng ZHAO ; Jing-Rong WEN ; Shu WANG ; Xue-Min SHI
Chinese Journal of Biotechnology 2005;21(6):934-941
In order to provide a complete picture of pathogenesis in cerebral ischemia, cerebral cortex in MCAO rats were analysed for alteration in their proteomes. Comparative proteome analysis was used to compare signal corresponding to individual cerebral cortex proteins on a two-dimensional gel between MCAO rats and the normal control (NC) group. After sample preparation, two-dimensional electroghoresis separated proteins were stained with Commassie Brilliant Blue. The image data were analyzed on a Dell computer using Image Master v 3.01 software. In cerebral cortex, 30 proteins were differentially expressed in MCAO rats compared with NC. There were 11 spots significantly increased, 15 spots significantly decreased and Adenylate kinase isoenzyme 1 was detected only in NC group, biliverdin reductase B, small inducible cytokine A4 [Precursor] only in MCAO group. Peroxiredoxin 2 divided into two points in MCAO6h group. In the end, this approach may lay a foundation for the further investigation of pathogenic mechanisms in cerebral ischmic injury.
Animals
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Brain Ischemia
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genetics
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metabolism
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Cerebral Cortex
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metabolism
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Electrophoresis, Gel, Two-Dimensional
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Gene Expression Profiling
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Infarction, Middle Cerebral Artery
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genetics
;
metabolism
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Nerve Tissue Proteins
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chemistry
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genetics
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metabolism
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Proteome
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Proteomics
;
methods
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Random Allocation
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Rats
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Rats, Wistar
10.Proteomic analysis of human cerebral cortex in epileptic patients.
Jong Pil EUN ; Ha Young CHOI ; Yong Geun KWAK
Experimental & Molecular Medicine 2004;36(2):185-191
Epilepsy affects more than 0.5% of the world population and is known to be associated with a large genetic component eliciting an electrical hyperexcitability in the central nervous system. However, its pathogenic mechanisms remain poorly understood. In order to gain greater molecular incite in the pathogenesis in epilepsy, we analyzed proteomes of human cerebral cortices. Quantitative proteome analysis was used to compare signals corresponding to individual proteins between epileptic cerebral cortices from patients with temporal lobe epilepsy and age-matched non-epileptic subjects. To minimize individual variations, gender and age of the patients were matched. Changes of several spots were consistent among 6 pairs of epileptic patients and nonepileptic subjects. One of the spots was identified as the mitochondrial type Mn-superoxide dismutase (Mn-SOD) confirmed by Western blot analysis with Mn-SOD antibody and enzyme activity assay. Such results were agreeable with chemical and physical parameters given by the 2-dimensional electrophoresis (2-DE) gel. Mn-SOD was consistently down-regulated in epileptic cerebral cortices compared with those of nonepileptic subjects. Our results demonstrate a clear link between pathogenesis of epilepsy and SOD. Additionally, we identified four proteins that were consistently over-expressed in all epileptic temporal neocortices specimens and the other four proteins that were found to be expressed less than non-epileptic control subjects. These proteomic data provide cellular markers in the understanding mechanism of the epilepsy pathogenesis.
Adult
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Biological Markers/analysis
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Brain Chemistry
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Case-Control Studies
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Cerebral Cortex/chemistry/*metabolism
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Down-Regulation
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Electrophoresis, Gel, Two-Dimensional
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Epilepsy/genetics/*metabolism
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Female
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Humans
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Male
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Middle Aged
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Mitochondria/chemistry/genetics/*metabolism
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Nerve Tissue Proteins/chemistry/genetics/*metabolism
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Proteomics
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Research Support, Non-U.S. Gov't
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Superoxide Dismutase/analysis/genetics/*metabolism
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Up-Regulation