Matrix Metalloproteinases ; Neovascularization, Pathologic ; Neovascularization, Physiologic

Humans ; Microvessels ; Neovascularization, Pathologic ; Neovascularization, Physiologic ; Plaque, Atherosclerotic ; pathology

Cells, Cultured ; Humans ; Neovascularization, Physiologic ; immunology ; Pericytes ; metabolism ; physiology

Humans ; Hypoxia-Inducible Factor 1, alpha Subunit ; Neovascularization, Physiologic ; Osteogenesis

Endothelial colony-forming cells (ECFCs), different from classical endothelial progenitor cells, are late endothelial progenitor cells with the capability to promote angiogenesis. Recent studies showed that ECFCs have a huge angiogenesis potential in the restoration of ischemic hearts, lungs or brains. They are also able to induce the expression of vascular related factor to promote angiogenesis in repair of limb ischemia or bone injury. Furthermore, ECFCs possess a strong homing effect for tumor, which is closely related to tumor occurrence, development and prognosis. Thus, ECFCs are a novel direction for vascular regeneration study, and may lead to ground-breaking progresses in fields of tissue regeneration and tumor.
Endothelial Cells ; cytology ; Humans ; Ischemia ; Neovascularization, Physiologic ; Stem Cells ; cytology

Angiogenin, as a member of the ribonuclease superfamily, is an angiogenic protein. The angiogenic ability of angiogenin plays an important role in many physical and pathological processes. Angiogenin can induce endothelial cell migration, proliferation, tubulation, as well as inhibition of cellular apoptosis. Angiogenin can be used to modulate the angiogenetic process of tissue engineered constructions via local delivery. Furthermore, angiogenin can also be regarded as a biomarker for diagnostic evaluation of malignancy, or as a target for cancer therapy. This paper presents a comprehensive overview of the angiogenic mechanisms of angiogenin, as well as its potential application in the process of wound healing and treatment of ischemic diseases and malignancy.
Cell Movement ; Humans ; Neovascularization, Pathologic ; Neovascularization, Physiologic ; Ribonuclease, Pancreatic ; therapeutic use

Collagen ; Drug Combinations ; Flow Cytometry ; methods ; Humans ; Laminin ; Neovascularization, Pathologic ; Neovascularization, Physiologic ; Proteoglycans

Angiogenesis is a process involving the growth of new blood vessels from pre-existing vessels though sprouting or other ways. It plays an important role in both physiological and pathological processes. Researches have found that platelets may contribute to angiogenesis as well. In this paper, we review the role of platelet in angiogenesis, especially the relationship with tumor angiogenesis, and discuss clinical implications of these findings.
Blood Platelets ; physiology ; Humans ; Neoplasms ; blood supply ; Neovascularization, Pathologic ; physiopathology ; Neovascularization, Physiologic ; physiology ; Vascular Endothelial Growth Factor A ; physiology

Mesenchymal stem cells (MSC) have the potential of multi-directional differentiation, and can recruit endothelial cells, promote their proliferation, migration and angiogenesis, improve blood perfusion and oxygen suppliment, and repair damaged tissue. Exosome secreted by MSC contain mother cell-specific proteins, lipids and nucleic acids, and acts as signaling molecule, playing an important role in cell communication, thereby altering target cell function. In this review, the biological characteristics of MSC and its exosome, the mechanism of promoting vascular regeneration in patients with ischemic diseases, and the mechanism of hypoxia-inducible factor-1α（HIF-1α） in the vascular ischemia of ischemic diseases are all summarized briefly.
Cell Differentiation ; Exosomes ; Humans ; Hypoxia-Inducible Factor 1, alpha Subunit ; Mesenchymal Stem Cells ; Neovascularization, Pathologic ; Neovascularization, Physiologic

OBJECTIVETo explore a new method for the therapy of avascular necrosis of the femoral head.

METHODSThe recombinant plasmid pCD-hVEGF165 was mixed with collagen and was implanted in the necrotic femoral head. The expression of vascular endothelial growth factor (VEGF) was examined by RNA dot hybridization and immunohistochemical techniques. Repair of the femoral head was observed by histological and histomorphometric analysis.

RESULTSThe expression of VEGF was detected in the femoral head transfected with the VEGF gene. The femoral head transfected with the VEGF gene showed a significant increase in angiogenesis 2 and 4 weeks after gene transfection and a significant increase in bone formation 6 and 8 weeks after gene transfection on histomorphometric analysis (P < 0.01).

CONCLUSIONSTransfection of the VEGF gene enhances bone tissue angiogenesis. Repair of osteonecrosis could be accelerated accordingly, thus providing a potential method for therapy of osteonecrosis.