No abstract available.
Diagnosis* ; Humans ; Liver Neoplasms* ; Liver Transplantation* ; Liver* ; Neoplasm Metastasis*

No abstract available.
Diagnosis* ; Humans ; Liver Neoplasms* ; Liver Transplantation* ; Liver* ; Neoplasm Metastasis*

Lymphoma is a kind of malignant tumors that takes place in the lymphoid and hematological system. It is important to establish appropriate and stable animal models of lymphoma and they are useful for the experimental research of mechanisms and efficient treatment of disease. In this article the establishment methods, characteristics and practical use of various animal models of lymphoma were reviewed.
Animals ; Disease Models, Animal ; Lymphoma ; Mice ; Mice, Nude ; Neoplasm Transplantation

Animals ; Camptothecin ; pharmacology ; Liver Neoplasms, Experimental ; immunology ; Mice ; Neoplasm Transplantation ; Sarcoma, Experimental ; immunology ; Transplantation, Isogeneic

Hematopoietic Stem Cell Transplantation ; Humans ; Multiple Myeloma ; pathology ; therapy ; Neoplasm Staging ; Transplantation, Autologous

Camptothecin/*pharmacology ; Liver Neoplasms, Experimental/*immunology ; Neoplasm Transplantation ; Sarcoma, Experimental/*immunology ; Transplantation, Isogeneic

Objective: To investigate the clinical significance of minimal residual disease (MRD) monitoring by using WT1 gene and flow cytometry (FCM) in patients with myelodysplastic syndrome (MDS) who receiving allogeneic stem cell transplantation (allo-HSCT). Methods: WT1 gene and MDS-related abnormal immunophenotype were examined by real-time quantitative polymerase chain reaction (RQ-PCR) and FCM, respectively. The bone marrow samples were collected from patients with MDS who received allo-HSCT from Feb, 2011 to Oct, 2015 in Peking University People's Hospital before and after transplantation. Results: Among 92 MDS patients, 40 (48.2%) patients were positive for WT1 (WT1(+)) and 9 (10.8%) patients were positive for flow cytometry (FCM(+)). 27 patients (29.3%) met the criteria of our combinative standard, MRDco (MRDco(+)). Only FCM(+) post-transplant (P<0.001) and MRDco(+) (P=0.017) were associated with relapse. The cumulative incidence of relapse (CIR) at 2 years were 66.7% and 1.2% (P<0.001) in FCM(+) and FCM(-) groups. MRDco(+) group had a 2-year CIR of 23.0% while MRDco(-) group had a 2-year CIR of 1.6% (P=0.004). The specificity of post-transplant WT1, FCM and MRDco to predict relapse was 59.0%, 96.4% and 74.7%, respectively. The sensitivity of these three MRD parameters to predict relapse was 66.7%. Conclusion: Post-transplant FCM and MRDco are useful tools to monitor MRD for MDS after transplantation. The preemptive intervention based on MRDco is able to reduce the relapse rate.
Flow Cytometry ; Hematopoietic Stem Cell Transplantation ; Humans ; Myelodysplastic Syndromes/therapy* ; Neoplasm Recurrence, Local ; Neoplasm, Residual ; Stem Cell Transplantation ; Transplantation, Homologous ; WT1 Proteins

OBJECTIVETo develop a human ovarian carcinoma SKOV3 model in severe combined immunodeficiency (SCID) mouse and to study its biologic characteristics.

METHODSHuman ovarian carcinoma SKOV3 cells were injected intraperitoneally into female SCID mouse to establish a transplantation model of human ovarian carcinoma. The biological characteristics, metastasis and morphology of transplanted tumors were studied.

RESULTAll tumors grew progressively with no sign of regression. The tumor cells spread around the peritoneal cavity and mainly on the diaphragm, mesentery, peritoneum and around the liver, which was confirmed by histopathology. The morphology, growth pattern and CA125 secretion of primary culture of transplanted cells remained as same as those of ovarian carcinoma cell line SKOV3.

CONCLUSIONAn intraperitoneal transplantation model of human ovarian carcinoma SKOV3 in SCID mice has been developed successfully, which can simulate the biological behavior of peritoneal metastasis of human ovarian carcinoma.

Animals ; Disease Models, Animal ; Female ; Humans ; Mice ; Mice, SCID ; Neoplasm Transplantation ; Ovarian Neoplasms ; pathology ; ultrastructure ; Peritoneal Neoplasms ; secondary ; Transplantation, Heterologous

OBJECTIVEThe aim of this study was to establish patient-derived lung cancer xenograft models in nude mice by subcutaneous and subrenal capsule transplantation, and to analyze the differences in biological characteristics of the xenografts.

METHODSSurgically resected lung cancer specimens from 11 patients were implanted subcutaneously and under the renal capsule in nude mice. The tumor growth and histopathological features were observed and human origin of the blood vessels in the first-generation xenograft tumors was evaluated by SP immunohistochemistry using anti-human CD31 antibody.

RESULTSThe patient-derived lung cancer tissues were successfully implanted subcutaneously and under the renal capsule in 11 nude mice. The operation time of subcutaneous implantation was 13 min, and the tumor formation rate was 36.4% (4/11). The operation time of implantation under the renal capsule was 45 min, and the tumor formation rate was 45.5% (5/11). Histopathological examination of the xenografts using HE staining showed the same morphology of the human lung cancers, and immunohistochemical observation with CD31staining showed that 83.3% (5/6) blood vessels in the xenograft tumors was of human origin.

CONCLUSIONSBoth methods of subcutaneous and subrenal capsule transplantation can be used to successfully establish patient-derived lung cancer xenograft models in nude mice. The subcutaneous implantation is simple to operate, less time-comsuming, and easy to observe the tumor growth, but with a lower success rate of tumor formation. Transplantation under the renal capsule has a higher tumor formation rate, but is more difficult to operate, taking more time, and difficult to observe the growth of the tumor. The xenograft tumors formed by both methods in the first generation display biological characteristics of human lung cancer, the xenograft tumor models are close to human lung cancer, and therefore may provide a stable, reliable, and useful animal model in human lung cancer research.

Animals ; Disease Models, Animal ; Heterografts ; Humans ; Immunohistochemistry ; Lung Neoplasms ; Mice ; Mice, Nude ; Neoplasm Transplantation ; methods ; Transplantation, Heterologous

OBJECTIVETo establish an subcutaneous xenotransplanted tumor model of human ameloblastoma in nude mice.

METHODSAmeloblastoma cells were absorbed by primary culture, repeat attachment and pancreas proteolytic enzyme were both used to purify them. Then, the purified cells were implanted subcutaneously into the nude mice. The specimens were respectively investigated by microscope in different spots after implanting.

RESULTSAmeloblastoma cells can survive in all of the 8 nude mice. The xenograft can be found on 23 days after implanting. The rate of successful inocutation is 25%. The subcutaneously xenotransplanted tumor cells can be found with microscope in the inter-muscle tissues of nude mice.

CONCLUSIONThe subcutaneously xenotransplanted tumor model of human ameloblastoma in nude mice was successfully established and it may benefit to further studies on this tumor.

Ameloblastoma ; Animals ; Disease Models, Animal ; Humans ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Neoplasm Transplantation ; Transplantation, Heterologous