1.Role of Whole Body FDG-PET in the Diagnosis of Hidden Distant Metastasis before Liver Transplantation in Patients with Primary Liver Cancer.
Won Woo LEE ; Jin Sook RYU ; You Jung YANG ; Jae Seung KIM ; Jeong Seok YEO ; Dae Hyuk MOON ; Sung Gyu LEE
Korean Journal of Nuclear Medicine 2002;36(6):368-380
No abstract available.
Diagnosis*
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Humans
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Liver Neoplasms*
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Liver Transplantation*
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Liver*
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Neoplasm Metastasis*
2.Role of Whole Body FDG-PET in the Diagnosis of Hidden Distant Metastasis before Liver Transplantation in Patients with Primary Liver Cancer.
Won Woo LEE ; Jin Sook RYU ; You Jung YANG ; Jae Seung KIM ; Jeong Seok YEO ; Dae Hyuk MOON ; Sung Gyu LEE
Korean Journal of Nuclear Medicine 2002;36(6):368-380
No abstract available.
Diagnosis*
;
Humans
;
Liver Neoplasms*
;
Liver Transplantation*
;
Liver*
;
Neoplasm Metastasis*
3.Advance of study on animal models of lymphoma.
Journal of Experimental Hematology 2009;17(5):1390-1393
Lymphoma is a kind of malignant tumors that takes place in the lymphoid and hematological system. It is important to establish appropriate and stable animal models of lymphoma and they are useful for the experimental research of mechanisms and efficient treatment of disease. In this article the establishment methods, characteristics and practical use of various animal models of lymphoma were reviewed.
Animals
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Disease Models, Animal
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Lymphoma
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Mice
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Mice, Nude
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Neoplasm Transplantation
7.Clinical implication of minimal residue disease monitoring by WT1 gene detection and flow cytometry in myelodysplastic syndrome with allogeneic stem cell transplantation.
Xiao Su ZHAO ; Xiao Dong MO ; Yan HONG ; Ying Jun CHANG ; Ya Zhen QIN ; Yan Rong LIU ; Yu hong CHEN ; Xiao Hui ZHANG ; Lan Ping XU ; Xiao Jun HUANG
Chinese Journal of Hematology 2018;39(12):998-1003
Objective: To investigate the clinical significance of minimal residual disease (MRD) monitoring by using WT1 gene and flow cytometry (FCM) in patients with myelodysplastic syndrome (MDS) who receiving allogeneic stem cell transplantation (allo-HSCT). Methods: WT1 gene and MDS-related abnormal immunophenotype were examined by real-time quantitative polymerase chain reaction (RQ-PCR) and FCM, respectively. The bone marrow samples were collected from patients with MDS who received allo-HSCT from Feb, 2011 to Oct, 2015 in Peking University People's Hospital before and after transplantation. Results: Among 92 MDS patients, 40 (48.2%) patients were positive for WT1 (WT1(+)) and 9 (10.8%) patients were positive for flow cytometry (FCM(+)). 27 patients (29.3%) met the criteria of our combinative standard, MRDco (MRDco(+)). Only FCM(+) post-transplant (P<0.001) and MRDco(+) (P=0.017) were associated with relapse. The cumulative incidence of relapse (CIR) at 2 years were 66.7% and 1.2% (P<0.001) in FCM(+) and FCM(-) groups. MRDco(+) group had a 2-year CIR of 23.0% while MRDco(-) group had a 2-year CIR of 1.6% (P=0.004). The specificity of post-transplant WT1, FCM and MRDco to predict relapse was 59.0%, 96.4% and 74.7%, respectively. The sensitivity of these three MRD parameters to predict relapse was 66.7%. Conclusion: Post-transplant FCM and MRDco are useful tools to monitor MRD for MDS after transplantation. The preemptive intervention based on MRDco is able to reduce the relapse rate.
Flow Cytometry
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Hematopoietic Stem Cell Transplantation
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Humans
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Myelodysplastic Syndromes/therapy*
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Neoplasm Recurrence, Local
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Neoplasm, Residual
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Stem Cell Transplantation
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Transplantation, Homologous
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WT1 Proteins
8.Biologic characteristics of intraperitoneal transplantation model of human ovarian carcinoma in severe combined immunodeficiency mice.
Zi-min PAN ; Da-feng YE ; Xing XIE ; Huai-zeng CHEN ; Wei-guo LÜ
Journal of Zhejiang University. Medical sciences 2003;32(5):423-426
OBJECTIVETo develop a human ovarian carcinoma SKOV3 model in severe combined immunodeficiency (SCID) mouse and to study its biologic characteristics.
METHODSHuman ovarian carcinoma SKOV3 cells were injected intraperitoneally into female SCID mouse to establish a transplantation model of human ovarian carcinoma. The biological characteristics, metastasis and morphology of transplanted tumors were studied.
RESULTAll tumors grew progressively with no sign of regression. The tumor cells spread around the peritoneal cavity and mainly on the diaphragm, mesentery, peritoneum and around the liver, which was confirmed by histopathology. The morphology, growth pattern and CA125 secretion of primary culture of transplanted cells remained as same as those of ovarian carcinoma cell line SKOV3.
CONCLUSIONAn intraperitoneal transplantation model of human ovarian carcinoma SKOV3 in SCID mice has been developed successfully, which can simulate the biological behavior of peritoneal metastasis of human ovarian carcinoma.
Animals ; Disease Models, Animal ; Female ; Humans ; Mice ; Mice, SCID ; Neoplasm Transplantation ; Ovarian Neoplasms ; pathology ; ultrastructure ; Peritoneal Neoplasms ; secondary ; Transplantation, Heterologous
9.Establishment of nude mouse models of patient-derived lung cancer xenograft.
Hong PAN ; Zhiqing MA ; Li MAO
Chinese Journal of Oncology 2014;36(8):571-574
OBJECTIVEThe aim of this study was to establish patient-derived lung cancer xenograft models in nude mice by subcutaneous and subrenal capsule transplantation, and to analyze the differences in biological characteristics of the xenografts.
METHODSSurgically resected lung cancer specimens from 11 patients were implanted subcutaneously and under the renal capsule in nude mice. The tumor growth and histopathological features were observed and human origin of the blood vessels in the first-generation xenograft tumors was evaluated by SP immunohistochemistry using anti-human CD31 antibody.
RESULTSThe patient-derived lung cancer tissues were successfully implanted subcutaneously and under the renal capsule in 11 nude mice. The operation time of subcutaneous implantation was 13 min, and the tumor formation rate was 36.4% (4/11). The operation time of implantation under the renal capsule was 45 min, and the tumor formation rate was 45.5% (5/11). Histopathological examination of the xenografts using HE staining showed the same morphology of the human lung cancers, and immunohistochemical observation with CD31staining showed that 83.3% (5/6) blood vessels in the xenograft tumors was of human origin.
CONCLUSIONSBoth methods of subcutaneous and subrenal capsule transplantation can be used to successfully establish patient-derived lung cancer xenograft models in nude mice. The subcutaneous implantation is simple to operate, less time-comsuming, and easy to observe the tumor growth, but with a lower success rate of tumor formation. Transplantation under the renal capsule has a higher tumor formation rate, but is more difficult to operate, taking more time, and difficult to observe the growth of the tumor. The xenograft tumors formed by both methods in the first generation display biological characteristics of human lung cancer, the xenograft tumor models are close to human lung cancer, and therefore may provide a stable, reliable, and useful animal model in human lung cancer research.
Animals ; Disease Models, Animal ; Heterografts ; Humans ; Immunohistochemistry ; Lung Neoplasms ; Mice ; Mice, Nude ; Neoplasm Transplantation ; methods ; Transplantation, Heterologous
10.Establishment of subcutaneous xenotransplanted tumor model of human ameloblastoma in nude mice.
Lei-tao ZHANG ; Hong-zhang HUANG ; Dong-lin ZENG ; Bin ZHANG
West China Journal of Stomatology 2007;25(1):12-14
OBJECTIVETo establish an subcutaneous xenotransplanted tumor model of human ameloblastoma in nude mice.
METHODSAmeloblastoma cells were absorbed by primary culture, repeat attachment and pancreas proteolytic enzyme were both used to purify them. Then, the purified cells were implanted subcutaneously into the nude mice. The specimens were respectively investigated by microscope in different spots after implanting.
RESULTSAmeloblastoma cells can survive in all of the 8 nude mice. The xenograft can be found on 23 days after implanting. The rate of successful inocutation is 25%. The subcutaneously xenotransplanted tumor cells can be found with microscope in the inter-muscle tissues of nude mice.
CONCLUSIONThe subcutaneously xenotransplanted tumor model of human ameloblastoma in nude mice was successfully established and it may benefit to further studies on this tumor.
Ameloblastoma ; Animals ; Disease Models, Animal ; Humans ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Neoplasm Transplantation ; Transplantation, Heterologous