Objective To investigate Caspase 3 activity in the ischemia reperfusion injury in rats and the protective effect of ischemic preconditioning and its possible mechanism.Methods 30 rats were randomly assigned to ischemia reperfusion (IR) group, ischemic preconditioning(IP) group,sham-operation(S)group.The serum aspartate transaminase (AST),alanine transaminase(ALT),liver caspase 3 activity,and apoptosis index(AI) of hepatocytes were examined in the three groups at 3 hours after repersusion.Results The serum AST,ALT,liver caspase 3 activity and apoptotic hepatocytes were significantly higher in both IP and IR groups than those in S group(P

Objective To compare the protective effect of ischemic preconditioning(IP) and caspase inhibitor on ischemia reperfusion(IR) injury of the liver in rats,and to investigate the possible mechanism. Methods SD rats subjected to 60 or 120 minutes of 70% hepatic ischemia and 3 hours of reperfusion,respectively,then divided randomly into 6 groups: (1)Ischemia/ reperfusion group 1 (IR 1 group);(2)IR 2 group;(3)Ischemia preconditioning group 1(IP 1 group);(4)IP 2 group; (5)Caspase inhibitor treatment group1(T 1 group);(6)T 2 group.Serum levels of aspartate aminotransferase (AST) and alanine aminotransfernase (ALT),and liver Caspase-3 activity, hepatocytes apoptosis,and animal seven-day(7 d) survive rate were determined. Results Both ischemic preconditioning and Caspase inhibitor treatment could protect the liver against IR injury,and their protective effect in IP 1 group and T 1 group was equally at 3h reperfusion after 60min ischemia. At 3h reperfusion after 120min ischemia, the effect of inhibiting Caspase-3 activity and decreasing hepatocyte apoptosis in T 2 group were significantly superior to those in IP 2 group(P0.05). Conclusions Both IP and Caspase inhibitor treatment can protect the liver against IR injury in rats,and the protective effect is no significant difference between the two methods.However IP is more simple?safe and cheaper comparing with Caspase inhibitor treatment.

Objective To analyze the expression of HLJ1 in the tissues of hepatocellular carcinoma(HCC) and the correlation between its expression and clinical pathological characteristic.Methods HLJ1 mRNA expression level was determined in 32 HCC tissues and their associated noncancerous liver tissues by reverse transcription polymerase chain reaction(RT-PCR).The correlation of HLJ1 expression level with clinicopathologic variables was also analyzed.Results HLJ1 mRNA expression level was significantly lower in HCC than in associated noncancerous liver tissue [(1.18?0.82) vs.(1.92?1.15),P

Objective To investigate the level of depression on interference suppression function in patients with coronary heart disease (CHD).Methods 15 CHD patients with major depression,21 CHD patients with mild depression and 24 CHD patients without depressive symptoms in the Affiliated Hospital of Weifang Medical University from May 2013 to March 2015 were selected to complete the Emotional Stroop task and record their accuracy and reaction time according to the Self-Rating Depression Scale (SDS) and Classification and Diagnostic Criteria of Mental Disorders in China-Third Edition(CCMD-3).Results ① The reaction time was longer (F(2,57) =71.97,P＜0.01)and the accuracy was lower (F(2,57)=8.78,P=0.04) in CHD with major depression group and CHD with mild depression group compared with CHD group.② The reaction time of negative words ((872.77±348.47) ms,(796.53±200.92)ms) was longer than positive words ((809.22±343.45)ms,(740.85±177.82)ms) in CHD with major depression group and CHD with mild depression group.③ The interference effect of negative words was bigger in CHD with major depression group and CHD with mild depression group ((35.48±181.97)ms,(7.16±200.06)ms) compared with CHD group ((-19.86± 177.82)ms).Conclusion Both CHD patients with major depression and CHD patients with mild depression have deficits in interference inhibition for negative information,which suggests that CHD with depressive individuals have impaired cognitive control function.

Objective To compare the clinical significance of peripheral blood neutrophil to lymphocyte ratio (NLR) and platelet to lymphocyte ratio (PLR) in predicting prognosis of patients with hepatocellular carcinoma (HCC).Methods The clinical data of 661 patients with HCC were retrospectively analyzed.Routine peripheral blood test results were used to calculate the NLR and PLR,and the receiver operating characteristic (ROC) curves were drawn.Using the thresholds of NLR and PLR,the patients were divided into the low NLR group and the high NLR group,and the low PLR group and the high PLR group.Overall survival (OS) and disease free survival (DFS) were evaluated by the Kaplan-Meier method.Independent prognostic predictors were determined by the Cox proportional hazard model.Results The NLR and PLR thresholds were 2.790 and 99,respectively.Analysis of the ROC curves showed higher NLR and PLR were significantly associated with poorer OS and DFS (all P ＜ 0.05).Multivariate analysis showed that NLR was an independent risk factor of OS and DFS (both P ＜ 0.05).The results remained unchanged when the NLR was further analyzed by applying different cut-off values of 2.810 and 3.In subgroup analysis,NLR remained an independent factor of Barcelona Clinical Liver Cancer staging system (BCLC) 0/A/B (P ＜ 0.05 for all measurements).Conclusion An elevated preoperative NLR could be a better prognostic predictor for HCC patients in comparison with PLR,especially for BCLC 0/A/B patients.

Objective To investigate the incidence and predictors of cerebral infarction in patients with ruptured ACoA aneurysms, and to provide diagnostic and therapeutic information.Methods A total of 319 patients with ruptured ACoA aneurysms in our hospital from January 2009 to February 2015 were reviewed in this study. The author collected data regarding clinical characteristics, and measured the aneurysm morphologies on CTA images. Age, flow angle, vessel angle were analyzed by independent-samples t tests in patients with or without cerebral infarction. Mann-Whitney U tests were used for aneurysm size, aneurysm height, perpendicular height, neck size, size ratio, aspect ratio, aneurysm angle , World Federation of Neurosurgical Societies (WFNS) grade at admission and Fisher grade. Chi-square tests and Fisher's exact tests were used for sex, histories of hypertension, smoking and stroke, treatment modalities, anterior cerebral A1 segment configuration and angiographic vasospasm on CTA images. The multivariate logistic regression analyses were used to determine the independent risk factors of cerebral infarction using the stepwise regression method. Results Of the 319 patients, there were 253 without and 66 patients with cerebral infarction. Differences of age(53±11 vs 57±12,respectively;t=-2.415, P=0.016), Fisher grade [Ⅰ 23(9.1%), Ⅱ 27(10.7%), Ⅲ 74(29.2%), Ⅳ 129(51.0%) vs Ⅰ 1(1.5%), Ⅱ 7 (10.6% ), Ⅲ 13(19.7% ), Ⅳ 45(68.2% ), respectively;Z=-2.541, P=0.035] and treatment modalities [endovascular coil embolization 155(61.3% ), neurosurgical clipping 98(38.7% ) vs endovascular coil embolization 23(34.8%), neurosurgical clipping 43(65.2%), respectively;χ2=14.810, P<0.001] reached statistical significance. Multivariate analysis showed that Fisher grade Ⅳ(OR=10.36,95%CI 1.34-80.29, P=0.025) and neurosurgical clipping (OR=3.28, 95% CI 1.84-5.86,P<0.001)still had statistical significance. Conclusions Cerebral infarction in patients with ruptured ACoA aneurysms may be associated with Fisher grade and treatment modalities. Although there is difference between the two groups in age, it is not a predictor of the occurrence of cerebral infarction.