AIM: To study the effect of cGMP-dependent protein kinase (PKG) on the pathogenesis of burn shock. METHODS: Confluent endothelial cells were disintegrated and centrifugated to obtain cell lysates after being treated with 10% burn serum or PKG activator 8-Br-cGMP. PKG activity of lysates was measured with radioactive isotope label method in a reaction system of phosphorylation of specific substrate H2B by PKG, and the shape and the distribution of intracellular filamentous actin were detected by specific fluorescence staining. For the control study, the PKG specific inhibitor KT5823 were used to pretreat the endothelial cells before the administration of burn serum or PKG activator 8-Br-cGMP. RESULTS: Exposures to burn serum and 8-Br-cGMP led to a rapid time-dependent increase in endothelial PKG activity and the polar distribution of intracellular filamentous actin, and preincubation with KT5823 abolished those effects. CONCLUSIONS: The results suggest that burn serum induces PKG activation and the stress variety of filamentous actin in the vascular endothelial cells, which probably contributes to the endothelial hyperpermeability after burn shock. [

OBJECTIVETo explore the anatomical basis and clinical application of axial subdermal vascular network skin flap (ASVNF) in the management of deep burn wounds.

METHODSTwelve samples of skin and subcutaneous tissue of lower extremities from adult cadavers were anatomically observed. The course, branching and the architecture of the subdermal vascular network (SVN) in the superficial facia and the ultra-thin area of the anterolateral femoral and saphenous axial subdermal vascular network skin flaps were intensively studied. The wounds in twenty-six patients with deep burn injury in the limbs were repaired with ASVNF.

RESULTSThe circular thinning method was employed in the preparation of anterolateral femoral ASVNF whose blood supply was provided by the musculocutaneous perforators. And the "U" shaped ultra-thin method was employed in the preparation of saphenous ASVNF, whose blood supply was directly provided by skin vessels. The flaps in these 26 patients survived very well in terms of the flap color, texture, contour and function after followed-up for 0.5 approximately 2.5 year.

CONCLUSIONThinning methods for different kinds of ASVNF should be individualized. ASVNF might be an optimal choice in the management of deep burn wounds due to its obvious advantages.

Adolescent ; Adult ; Burns ; surgery ; Dermis ; anatomy & histology ; blood supply ; Female ; Humans ; Male ; Middle Aged ; Skin Transplantation ; methods ; Surgical Flaps ; blood supply ; Time Factors ; Wound Healing

OBJECTIVETo explore the effect of exogenous putrescine on renal function and cell apoptosis in rats.

METHODSNinety SD rats were randomized into control group (n=10), high-dose putrescine group (P1 group, n=40), and low-dose putrescine group (P2 group, n=40) with intraperitoneal injections of 2 ml of normal saline, 50 µg/g putrescine, and 25 µg/g putrescine, respectively. At 24, 48, 72 and 96 h after the injections, 10 rats from each group were sacrificed to examine serum Cr and BUN levels, histological changes in the kidneys, and renal cell apoptosis (TUNEL assay).

RESULTSThe rats in the two putrescine- treated groups showed mild edema in some renal tissues without obvious necrosis. In P1 and P2 groups, serum Cr and BUN levels differed significantly at each time point of measurement (P<0.01 and P<0.05, respectively), and were significantly higher than the levels in the control group (P<0.01 and P<0.05, respectively). The two putrescine-treated groups showed gradually increased renal cell apoptosis with time, reaching the peak levels at 96 h and 48 h, respectively. The peak renal cell apoptosis rates in P1 [(24.78∓2.19)%] and P2 [(26.27∓2.13)%] group were significantly higher than the rate in the control group [(4.47∓0.33)%, P<0.01].

CONCLUSIONExogenous putrescine can lead to renal function impairment and induce renal cell apoptosis in rats, and the severity of these changes appeared to be associated with the blood concentration of exogenous putrescine.

Animals ; Apoptosis ; drug effects ; Kidney ; drug effects ; physiopathology ; Putrescine ; adverse effects ; blood ; Rats ; Rats, Sprague-Dawley

Whole esophagus deep burn is an extremely rare upper gastrointestinal tract disease. We report a case of severe burns of involving extensive body skin, eyes, throat, and esophagus. Endoscopic examination revealed acute necrotizing esophagitis and detected a metal foreign body in the stomach. The patient underwent burn wound debridement with analgesia, anti-shock rehydration, anti-infection, and symptomatic treatments, which failed to improve the conditions. The patient died of respiratory and circulatory failure secondary to serious sepsis.
Burns ; complications ; Esophagus ; injuries ; Foreign Bodies ; complications ; Humans ; Male ; Middle Aged ; Stomach

Whole esophagus deep burn is an extremely rare upper gastrointestinal tract disease. We report a case of severe burns of involving extensive body skin, eyes, throat, and esophagus. Endoscopic examination revealed acute necrotizing esophagitis and detected a metal foreign body in the stomach. The patient underwent burn wound debridement with analgesia, anti-shock rehydration, anti-infection, and symptomatic treatments, which failed to improve the conditions. The patient died of respiratory and circulatory failure secondary to serious sepsis.

Whole esophagus deep burn is an extremely rare upper gastrointestinal tract disease. We report a case of severe burns of involving extensive body skin, eyes, throat, and esophagus. Endoscopic examination revealed acute necrotizing esophagitis and detected a metal foreign body in the stomach. The patient underwent burn wound debridement with analgesia, anti-shock rehydration, anti-infection, and symptomatic treatments, which failed to improve the conditions. The patient died of respiratory and circulatory failure secondary to serious sepsis.