Intestinal mucosa plays important roles in digestion, absorption and substance exchange between organism and external environment. Meanwhile, it is the largest immune organ and mucosal barrier, including mechanical, biological and immune barrier. A variety of diseases, especially postoperative complications, are associated with the damage of mucosal barrier. Esophageal cancer surgery is complex and many perioperative factors, especially hypoperfusion and fasting, may affect the integrity of intestinal barrier. Understanding of the mechanism of intestinal barrier (mechanical, biological and immune barrier), the physiological function of probiotics, and the benefit of early enteral nutrition to intestinal barrier are important components to achieve fast recovery after surgery for esophageal cancer.
Enteral Nutrition ; Esophageal Neoplasms ; surgery ; therapy ; Humans ; Intestinal Mucosa ; Perioperative Period

Decision Making ; Esophageal Diseases ; diagnosis ; therapy ; Humans ; Physician's Role ; Thoracic Surgical Procedures

Esophageal Neoplasms ; mortality ; surgery ; Humans ; Quality of Life ; Survival Analysis

Objective To prepare monoclonal antibody (mAb) against recombinant human cardiac troponin I(cTnI).Methods The full-length gene encoding human cardiac troponin I (cTnI) was synthesized chemically and inserted into expression plasmid pBV220 to construct recombinant plasmid p pBV220/cTnI. The recombinant plasmid was transformed into E.coli DH5? which then expressed cTnI. The immunological activity of the expressed cTnI was analyzed by Western blot. Recombinant human cTnI protein was used as antigen to immunize BALB/c mice. Monoclonal anti-bodies against cTnI were prepared by normal hybridoma technology. The relative affinity of mAbs was determined by ELISA. Specificity of mAbs was analyzed by Western blot.Results Human cTnI gene was synthesized and confirmed by DNA sequencing. Positive recombinant clones were identified by restriction enzyme digestion analysis and DNA sequencing. Western blot analysis showed that the cTnI protein could be recognized by an anti- cTnI antibody. Two hybridmas producing antibodies against cTnI were obtained. IgG isotypes of two mAbs were IgG2a and IgG2b. Western blot showed that the antibodies were specific for cTnI. Neutralisation test showed that these mAbs could be evidently neutralized by cTnI.Conclusion The recombinant expression plasmid of cTnI was constructed successfully and expressed in E.coli. The method of EL ISA established to test serum cTnI is to clinically useful. The cTnI mAb which using cTnI as antigen prepared in this paper can be used for cTnI immunoassay in vitro.

Proteomics analysis of multiple sclerosis will provide more molecular pathology information about it.With appropriated methods of sample preparation,protein separation and identification,some proteins related to multiple sclerosis could be found and helpful for diagnosis,treatment and prophylaxis.This will improve the final diagnosis ratio of suspected cases and offer clue of drug and gene therapy exploration.Intervention of the related protein ameliorate symptom and release relapse.

Objective: To observe the influence of silencing MAT1 gene by short interfering RNA (siRNA) on the proliferation and invasion of human pancreatic cancer cells and discuss the feasibility of using MAT1 gene as therapeutic target for treatment of pancreatic cancer. Methods: BxPC-3 cells were transfected with sequence-specific siRNA targeting MAT1 gene with liposome mediation. The mRNA and protein expression of MAT1 were confirmed by RT-PCR and Western blot, respectively. The cell proliferation was measured by Trypan blue exclusion staining. The invasion ability was determined by Boyden chamber model in vitro. Results: The mRNA and protein expression of MAT1 were significantly down-regulated by siRNA in BxPC-3 cells compared with the control groups. The expression of MAT1 mRNA was reduced by 55.2% and 64.3% at 24 h and 48 h, respectively (P <0.01). The cell proliferation and invasion ability of BxPC-3 cells were significantly inhibited in vitro (P < 0.01). Conclusion: The results suggest that siRNA targeting MAT1 gene inhibits the cell proliferation and invasion of BxPC-3 cells in vitro. MAT1 may be a potential target for gene therapy of human pancreatic cancer.

Objective To synthesize a new reagent, 1-(6-bromo-2-benzothiazolyl)-3-(5-bromo-8-quinolyl)-triazene (BBTBQT) and apply to the determination of cobalt. Methods The reagent had been synthesized by diazotization and coupling reaction. After purification and characterization, BBTBQT was tested for its color reaction conditions with cobalt in the presence of cetylpyridinium bromide (CPB). Results In the presence of borax buffer solution at pH value of 9.0, the reagent reacted with cobalt to form a blue stable complex with a molar ratio of 2∶1; the complex had a maximum absorption at 640 nm. The apparent molar absorptivity of the complex is 1.55 ?105 L/(mol?cm). Beer’s law was obeyed in the range of 0-0.28 mg/L for cobalt. The method had been applied to determine cobalt in drainage sediment and vitamin B12 injection solution with a mean relative error of 1.9%-5.8% and a standard relative deviation of 1.7%-3.8%. Conclusion The present method is selective, sensitive, accurate, convenient and suitable for determination of trace cobalt in the samples.

Aim To study the effect of Vitamin C on growth and apoptosis of human cervical cancer cell line HeLa and its mechanisms. Methods HeLa cell line was treated with VitC. Cell proliferation was evaluated with MTT assay. The distribution of cell cycle,the rate of apoptosis and the expression of HPV18 E6,p53,Bax and Bcl-2 protein of the heLa cells, were observed using flow cytometer respectively. TRAP-PCR-ELISA assay was used to evaluate telomerase activity. Results Vitamin C arrested the HeLa cell line in G_0/G_1 phase in a dose and time-dependent manner, and significantly inhibited HeLa cells growth and increased the rate of apoptosis.Expression of HPV18 E6 and Bcl-2 proteins, was down regulated expression of p53 and Bax proteins was upregulated, and the telomerase activity was reduced. Conclusion Vitamin C can significantly inhibit HeLa cells growth and proliferation,arrest them in G_0/G_1 phase and induce their apoptosis.The reason of these changes is the decrease of E6 gene′s expression, and the succedent changes of proteins′ expression in cancer cells,including the downregulated expression of HPV18 E6 and Bcl-2 proteins, upregulated expression of p53 and Bax proteins and reduced telomerae activity.

To get formed of the status of research and application of the domestic behavior therapy and its development trend, the time distribution and the subject distribution were bibliometrically analyzed of the literature on behavior therapy from 1981 to 2000 in the CBMdisc. Our results showed that the number of literature of behavior therapy has been increasing in exponential manner over the past 20 years; the behavior modification, the biofeedback and the cognitive therapy are extensively used in China. In clinical practice, the behavior modification and the biofeedback have been applied in all departments of medical institutions, especially for treating the cardiovascular and the neurological conditions. The cognitive therapy has been employed mainly for the treatment of mental disorders (or dysphrenia), the aversive therapy mainly for material withdrawal, and the systematic desensitization for phobia. There was no report found on the clinical use of meditation. It is concluded that the study and application in behavior therapy in China is currently developing very fast.
*Behavior Therapy/trends ; *Bibliometrics ; Biofeedback (Psychology) ; China ; Cognitive Therapy ; Heart Diseases/therapy ; Phobic Disorders/therapy ; Schizophrenia/*therapy

Ohjective To approach the therapeutic methods of urinary fistula after hypospadias urethroplas- ty.Methods Forteen post-hypospadia repair urethral fistula managed between 2000 to 2005 were reviewed.Differ- ent procedures were carried out according to the size and location of orificium fistulae.Results Eleven repairs were successful after one attempt.The success rate was 78% (11/14).Conclusion The procedure of fistula repair should be chosen according to the size,location and regional condition of orificium fistulae.