1. A new angiotensin-converting enzyme inhibitor from Peperomia pellucida (L.) Kunth
Islamudin AHMAD ; Neneng AMBARWATI ; Berna ELYA ; Arry YANUAR ; Abdul MUŃIM ; Hanita OMAR ; Kamarza MULIA ; Osamu NEGISHI ; Abdul MUŃIM
Asian Pacific Journal of Tropical Biomedicine 2019;9(6):257-262
Objective: To isolate, identify, and evaluate a new angiotensin-converting enzyme inhibitor from Peperomia pellucida (L.) Kunth herbs. Methods: A dried sample of Peperomia pellucida herb was successively macerated with n-hexane and ethyl acetate. The ethyl acetate extract solution was evaporated to obtain the crude extract. Vacuum liquid column chromatography and thin layer chromatography were performed to obtain two pure compounds. Then, both compounds were elucidated and identified using the spectroscopic method. Angiotensin-converting enzyme inhibitory activity studies of both compounds were determined using angiotensin-converting enzyme kit WST-1 with spectrophotometer microplate reader 96-well at 450 nm wavelength. Results: Two bioactive compounds were successfully isolated from Peperomia pellucida herb, including a new compound of 2,3,5-trimethoxy-9-(12,14,15-trimethoxybenzyl)-1H-indene and pellucidin A. Both compounds demonstrated angiotensin-converting enzyme inhibitory activity, with IC
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