1.Peptide Mimotopes of Neisseria meningitidis Group B Capsular Polysaccharide.
Inho PARK ; In Hong CHOI ; Se Jong KIM ; Jeon Soo SHIN
Yonsei Medical Journal 2004;45(4):755-758
The antigenic similarity between Neisseria meningitidis group B (NMGB) capsular polysaccharide (PS) and human polysialic acid (PSA) has hampered the development of a NMGB PS-based vaccine. But the possibility of a safe vaccine based on NMGB PS has been demonstrated by the existence of the NMGB PS-associated nonautoreactive epitope, which is distinct from those present on human PSA. To obtain peptide mimotopes of NMGB PS, we used HmenB3, a protective and nonautoreactive monoclonal antibody, to screen a phage library with 12 amino acids. We obtained 23 phage clones that bound to HmenB3 but not in the presence of E. coli K1 PS [which is alpha (2-8) -linked PSA like NMGB PS]. The clones contained 3 mimotopes and differed from previously described NMGB PS mimotopes. Immunization with a synthetic peptide of one mimotope elicited anti-NMGB antibodies in BALB/c mice. These mimotopes may be useful in the development of group B meningococcal vaccines.
Amino Acid Sequence
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Animals
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Bacterial Vaccines/*immunology
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Cloning, Molecular
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Female
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Meningococcal Infections/*immunology/*prevention & control
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Mice
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Mice, Inbred BALB C
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Molecular Sequence Data
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Neisseria meningitidis, Serogroup B/genetics/*immunology
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Polysaccharides, Bacterial/genetics/*immunology
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Research Support, Non-U.S. Gov't