1.Bacterial Succession on Rat Carcasses and Applications for PMI Estimation.
Lin ZHANG ; Juan-juan GUO ; TELET-SIYIT ; Yu-long PENG ; Dan XIE ; Ya-dong GUO ; Jie YAN ; Lagabaiyila ZHA ; Ji-feng CAI
Journal of Forensic Medicine 2016;32(1):1-6
UNLABELLED:
Abstract:
OBJECTIVE:
To investigate the bacterial succession on rat carcasses and to evaluate the use of bacterial succession for postmortem interval (PMI) estimation.
METHODS:
Adult female SD rat remains were placed in carton boxes. The bacterial colonization of circumocular skin, mouth and vagina was collected to be identified using culture-dependent biochemical methods. The changes in community composition were regularly documented.
RESULTS:
The bacterial succession in three habitats showed that Staphylococcus and Neisseria were predominated in early PMI, especially Staphylococcus aureus and Neisseria lactamica in 6 hours after death. Lactobacillus casei developed on the 3-4 days regularly, and kept stable at a certain level in late PMI.
CONCLUSION
The involvement of normal and putrefactive bacteria in three body habitats of rat remains can be used for PMI estimation.
Animals
;
Autopsy
;
Cadaver
;
Death
;
Forensic Medicine/methods*
;
Neisseria lactamica
;
Postmortem Changes
;
Rats
;
Rats, Sprague-Dawley
;
Staphylococcus aureus
;
Time Factors