From Nov. 1999 to April 2000, at Ha Noi Centre of Vereno-Dermatology, 365 samples isolated from patients of all ranges of age were studied, using the procedures of antibiogramme to determine the sensitivity of antibiotics to blennorrhagic agent. Results: 65 positive cases (17.8%), among them patients aged 16-40 account for highest positive rate (27.3%). Gonococcus has relatively high resistance to Penicillin (75.4%), then tetracyclin (21.5%), ciprofloxacin (6.2%). The resistance to Kanamycin (18.5%), Nalidizic acide (10.8%), Chloramphenicol (7.7%), Erythromycine (1.5%) were recorded. Multiresistance to 2 antibiotics had the highest rate: PG-TE (16.9%), PG-KA (15.4%), to 3 antibiotics PG-KA-NA (4.6%) was noted. There is no resistance to Ceftriaxon, Spectinomycin, Cephalexin
Neisseria gonorrhoeae ; Neisseria ; Gonorrhea

From 1992 to 2001, penicillin resistance of betalactamase-producing Neisseria gonorrhoeae was supervised by Institute of Dermato-Venereology. Results: the rate of penicillin-resistant gonococcal strains was highest in1995 (about 76%), and in 1996 (73.11%), especially in 3 years (1999-2001) this rate decreased significantly: 51.3%, 47.10%, and 35.54%, respectively
Neisseria gonorrhoeae ; Neisseria ; penicillinase

In the year 1997, at the National Institute of Dermato-Venerology, 2025 patients with the syndrome of vaginal and excretion underwent direct examinations and bacterial culture. Their 156 (7.7%) had positive reactions of blenorrhagy. Test of sensitivity on 156 strains of isolated gonoccoccus to antibiotics showed a rate of resistance to penicillin of 76.92%, tetracyclin 35.9%. All the isolated strains were sensitive to ceftriaxone 100%, spectinomycine 100% and 98.44% to cephalothine, 95.51% to chloramphenicol, 93.42% to ciprofloxacine. There was a PPNG rate of 64.2%
Neisseria gonorrhoeae ; Anti-Bacterial Agents ; Neisseria

In 1982, we reported a case of recalcitrant urethritis caused by a strain of spectinomycin resistant PPNG. It caused a great fear that it might be the signal of an end of spectinomycin era, The disk-prediffusion method was used on all isolates from the Choong-Ku VD Clinic between August 1984 and December 1985 to screen out strains of N. gonorrhoeize resistant to spectinomycin, with disks containing 100pg of spectinomycin, Strains with inhibition zone greater tha.n 25 mm were considered susceptible, We could not disclose a single strain which wa.s resistant to spectinornycin, The reason why we do not see any more of spectinomycin. N. gonorrhoeae requires some speculation, It is possible that the mutant which had resistant to spectinornycin might not have teen quite stable and, after series of passage through human hosts, mutated back to original spectinornycin sensitive states. It is suggested that continual surveillance for spectinomycin resistant N. gonorrhoeae should be conducted.
Adolescent ; Humans ; Neisseria gonorrhoeae* ; Neisseria* ; Spectinomycin* ; Urethritis

Penicillinase producing Neisseria gonorrhoeae(PPNG) have been reported from various cauntries throughout the world. We have been tr ying to assess the prevalence of PPNG in Korea since May, 1980 by means of chromogenic cephalosporin method. The total number of isolated Neisseria gonorrhoeae strains in 1983 was 733, among which 679 were tested for p-lactamase production, among which 178(26. 2%) were PPNGs. In 1984, 791 strains were isolated, among which 214(27.1%) were PPNGs. In all, 392(26.7%) strains were found to be PPNGs arnong 1470 Neisseria gonorrhoeae strains tested between 1983-1984.
Korea* ; Neisseria ; Neisseria gonorrhoeae ; Penicillinase ; Prevalence* ; Seoul*

Study on 943 patients with syndrome of exudate urethrovaginal, conjunctiva pus at National of Dermatology and venerology Hospital. Isolated 135 types of gonococci (14,31% positive). Using transplant and isolate bacterium techique on blood agar for antibiotic draft. Find out resistant to antibiotic rate of gonococci in Hanoi quite high in most of tested antibiotics. Penicillin was most of resistanced antibiotic 75,55, tetracyclin was 20,74%. In Ha noi occurred types of multi resistance gonococci with 2 antibiotic and 3 antibiotic. Not find any gonococci which resistant to spectinomycin and ceftriaxon in Hanoi
Epidemiology ; Neisseria gonorrhoeae ; diagnosis

BACKGROUND: Although the culture of urethral discharge using a cutton swab is regarded as one of the most sensitive and reliable diagnostic methods of gonorrhea, it may give pain or diagnostic methods of gonorrhea, it may give pain or discomfort to patients, and its detection rate may be influenced by the examiner's technical ability. OBJECTIVE: The purpose of this study was to compare the positive culture rates between ure4thral discharge and urinary sediment for the diagnosing of Neisseria gonorrheae. METHODS: We compared this technique with the culture of urethral discharge using the specimens from 357 male patients with gonococcal infection at VD clinic of Jungku Pvblic Health center in Seoul from Jan 1991 to Jun 1993. RESULTS: The culture of urinary sediment was positive in 339 of 357(94.95), whereas the culture of urethral discharge was positive in 346 of 357(96.95). However, these data have no statistical significance(P<0.05). The obe observed sensitivity and specificity of culture of urinary sediment comjpared to culture of urethral discharge were 97.75 and 90.9%, respectively. The predictive value of positive and negative test compared to the culture of urethral discharge were 99.7% and 55.65, respectively. As our data showed low negative predictive value, a negative culture result of urinary sediment does not always imply a negative culture result of urethral discharge. CONCLUSION: Therefore, at present, the previous method of the culture of urethral discharge is more highly recommended and further studies for this diagnostic and/or new method of gonorrhea based on larger group of patients is encouraged.
Gonorrhea ; Humans ; Male ; Neisseria gonorrhoeae* ; Neisseria* ; Sensitivity and Specificity ; Seoul

No abstract available.
Antibodies* ; Chlamydia trachomatis* ; Chlamydia* ; Humans ; Neisseria gonorrhoeae* ; Neisseria* ; Prevalence*

No abstract available.
Antibodies* ; Chlamydia trachomatis* ; Chlamydia* ; Humans ; Neisseria gonorrhoeae* ; Neisseria* ; Prevalence*

BACKGROUND: Molecular epidemiological typing of Neisseria gonorrhoeae is crucial for monitoring the spread of resistant strains. As reference strains can be used for laboratory internal quality control, we genetically characterised the American Type Culture Collection (ATCC) gonococcal strains by Neisseria gonorrhoeae multiantigen sequence typing (NG-MAST) and porB sequence typing using public multilocus sequence typing (PubMLST). METHODS: Eight ATCC gonococcal reference strains (ATCC 19424, ATCC 31426, ATCC 35541, ATCC 43069, ATCC 43070, ATCC 49226, ATCC 49926, and ATCC 49981) from Culti-Loops (Thermo Fisher Scientific, USA) were cultured. After DNA extraction, porB and tbpB were amplified and sequenced. Sequence types (STs) and allele numbers were each determined by NG-MAST (http://www.ng-mast.net) and porB sequence typing using PubMLST (http://pubmlst.org/neisseria/porB/). RESULTS: ATCC 19424 was identified as ST 266 by NG-MAST, and as Allele 946 by PubMLST. ATCC31426 was assigned a novel ST by NG-MAST, and was assigned Allele 958 with 1.2% mismatch by PubMLST. ATCC 35541 was identified as ST 12 by NG-MAST, and as Allele 624 by PubMLST. ATCC 43069 and ATCC 43070 were both identified as ST 681 by NG-MAST, and as Allele 984 by PubMLST. ATCC 49226 was identified as ST 1572 by NG-MAST, and as Allele 2110 by PubMLST. ATCC 49926 and ATCC 49981 were both identified as ST 16496 by NG-MAST, and as Allele 928 by PubMLST. CONCLUSIONS: The ST data obtained for ATCC gonococcal reference strains by NG-MAST and porB sequence typing using PubMLST can be used for quality assurance of molecular epidemiological typing in clinical microbiological laboratories.
Alleles ; DNA ; Multilocus Sequence Typing ; Neisseria gonorrhoeae ; Neisseria ; Quality Control