1.The study of the correlation of middle meatus volume and maxillary fungal ball.
Haiyan LI ; Jianping LIANG ; Hong YUAN ; Yipu MAO
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2014;28(23):1860-1862
OBJECTIVE:
Observing the anatomic variation and measuring the bone volume of meatus and nasal cavity by analyzing the expression of paranasal sinus CT. Searching whether these variation and volume data are related to maxillary fungal ball.
METHOD:
Measuring the double side bone volume of middle meatus,nasal cavity and the rate of middle meatus volume in the same side of nasal cavity respectively in the normal group, the maxillary fungal ball group. Observing the anatomic variation and statistically evaluating the anatomic variation and volume of nasal cavity and nasal meatus.
RESULT:
In the maxillary fungal ball group, the affected side and the contralateral side volume of middle meatus,nasal cavity and the rate of middle meatus volume in the nasal cavity had no statistical difference (P>0.05); Comparing the middle meatus volume and the rate of middle meatus of the maxillary fungus ball group affected side and normal group,there was statistical difference (P<0. 05). In the maxillary fungal ball group and the normal group, the morbidity of deviation septum were 24. 24% and 33. 33%, the morbidity of OMC variation were 30. 3% and 26. 67% (P<0.05), the morbidity of nasal anatomic variation were 54. 55% and 60.00%, there was no statistical difference (P>0.05).
CONCLUSION
Maybe there is a correlation between the enlarged bone middle meatus and the maxillary fungal ball. There is no relationship between the nasal anatomic variation and the maxillary fungal ball.
Humans
;
Mycoses
;
etiology
;
Nasal Cavity
;
anatomy & histology
;
microbiology
;
Nasal Septum
;
Paranasal Sinuses
2.Pathologic diagnosis of early syphilis in nasal cavity and oropharynx.
Sheng-zhong ZHANG ; Hong-gang LIU ; Ming LI ; Quan ZHOU ; Shao-hui SHI
Chinese Journal of Pathology 2006;35(7):403-406
OBJECTIVETo study the pathologic changes in nasal and oropharyngeal mucosa caused by treponema pallidum (TP) infection.
METHODSTwenty-five cases of nasal and oropharyngeal syphilis were retrieved from the archival files of Department of Pathology of Beijing Tong Ren Hospital collected during the period from June 1996 to September 2005. The hematoxylin and eosin-stained slides were reviewed. Histochemical study using modified Warthin-Starry stain and immunohistochemical study using polyclonal antibody for TP were carried out. The diagnosis of early syphilis was confirmed by rapid plasma regain (RPR) and TP hemagglutination (TPHA) tests.
RESULTSAmong the 25 cases studied, 20 showed neutrophil infiltration, microabscess formation and plasma cell infiltration in the lamina propria. Endothelial swelling of small blood vessels and syphilitic vasculitis was also seen. Tonsillar ulcers associated with abundant plasma cells, lymphocytes and histiocytes were noted in 14 cases. One of which demonstrated florid reactive lymphoid proliferation, with transforming lymphoid cells of various stages identified. Pseudoneoplastic squamous cell proliferation was seen in one case. Spirochetes were detected by modified Warthin-Starry stain in mucosal microabscesses and squamous epithelium in 20 cases, around small blood vessels in 5 cases, and on the surface of tonsillar ulcers in 14 cases. Abundant TP were also found in smears of exudates in 6 cases. TP antigen was detected in 4 cases by immunohistochemical staining. All the 25 cases studied were RPR (1:8 to 128) and TPHA-positive.
CONCLUSIONSEarly syphilis involving nasal cavity and oropharynx has distinctive pathologic features. Detailed histologic examination, together with modified Warthin-Starry stain for demonstration of spirochetes, is important to obtain a correct diagnosis.
Adult ; Aged ; Diagnosis, Differential ; Female ; Humans ; Male ; Middle Aged ; Nasal Cavity ; microbiology ; pathology ; Nasal Mucosa ; microbiology ; pathology ; Nose Diseases ; microbiology ; pathology ; Palatine Tonsil ; microbiology ; pathology ; Pharyngeal Diseases ; microbiology ; pathology ; Syphilis ; microbiology ; pathology ; Syphilis Serodiagnosis ; Treponema pallidum ; isolation & purification ; Young Adult
3.Identification of Vaginal Fluid Using Microbial Signatures.
Kai Nan ZOU ; Meng HU ; Jiang Ping HUANG ; Huai Gu ZHOU
Journal of Forensic Medicine 2016;32(4):254-256
OBJECTIVES:
To investigate the specific microbial signatures in vaginal fluid.
METHODS:
Vaginal fluid (16 samples), saliva (16 samples), feces (16 samples), semen (8 samples), peripheral blood (8 samples), urine (5 samples), and nasal secretion (4 samples) were collected respectively. The 16S rRNA genes of Lactobacillus crispatus, Lactobacillus gasseri, Lactobacillus jensenii, Lactobacillus iners, and Atopobium vaginae were amplified. PCR production was detected via a 3130xl Genetic Analyzer.
RESULTS:
The detected number of Lactobacillus crispatus, Lactobacillus gasseri, Lactobacillus jensenii, Lactobacillus iners, and Atopobium vaginae were 15, 5, 8, 14, and 3 in all vaginal fluid samples, respectively. Lactobacillus crispatus and Lactobacillus jensenii existed specifically in vaginal fluid.
CONCLUSIONS
There is a potential application value to detect Lactobacillus crispatus and Lactobacillus jensenii for the identification of vaginal fluid.
Actinobacteria/classification*
;
Blood/microbiology*
;
Body Fluids/microbiology*
;
Feces/microbiology*
;
Female
;
Genes, Bacterial
;
Humans
;
Lactobacillus/classification*
;
Nasal Cavity/microbiology*
;
Polymerase Chain Reaction
;
RNA, Ribosomal, 16S/genetics*
;
Saliva/microbiology*
;
Semen/microbiology*
;
Vagina/microbiology*
4.Homology of Staphylococcus aureus isolated from skin lesions and nose of patients with impetigo.
Yun-Zhu LI ; Lin MA ; Fan-Rong KONG
Acta Academiae Medicinae Sinicae 2009;31(1):10-12
OBJECTIVETo investigate the homology of Staphylococcus aureus (SA) strains isolated from nose and skin lesions of impetigo children.
METHODSTotally 263 outpatients aged 3 months to 14 years who were seen by the Department of Dermatology of Beijing Children's Hospital between August 2005 and March 2006 were enrolled in this study. The isolations from nose and skin lesions of 58 impetigo children who were randomly selected from these 263 children with spa sequence were typed. The sequence results of SA were analyzed using special websites.
RESULTSThere were 106 impetigo patients in these 263 children. The isolation rate of SA was 78.3% in the nose of 106 impetigo patients and was 21.0% in that of the rest 157 patients (P < 0.01). The age of all nasal carriers was concentrated in 1-6 years. Among the 106 impetigo patients, 30 patients had their primary lesions on the face (including 28 cases of SA nose isolates) and 76 patients had their primary lesions on the other parts of body (including 56 cases of SA nose isolates) (P < 0.01). The spa typing showed that 26 of the 30 impetigo patients had the same type pairs of nose and skin.
CONCLUSIONSSA isolated from the skin lesions and nose of impetigo patients has remarkably homology. Nasal carriage of SA may be closely relevant with the occurrence of impetigo.
Adolescent ; Child ; Child, Preschool ; Female ; Humans ; Impetigo ; microbiology ; Infant ; Male ; Nasal Cavity ; microbiology ; Sequence Homology ; Skin ; microbiology ; Staphylococcus aureus ; classification ; genetics ; isolation & purification
5.Analysis of Reporting Time for Identification of Methicillin-Resistant Staphylococcus aureus Carriers Using ChromID MRSA.
Yirang LEE ; Jae Seok KIM ; Han Sung KIM ; Hyun Soo KIM ; Wonkeun SONG ; Kyu Man LEE
Annals of Laboratory Medicine 2014;34(3):240-242
We assessed the reporting times for identification of nasal methicillin-resistant Staphylococcus aureus (MRSA) carriers in 2011 in a university-affiliated hospital using surveillance cultures incubated for 1 and 2 days with ChromID MRSA (bioMerieux, France). Of 2,732 nasal swabs tested, MRSA was detected in 829 (85.6%) and 140 (14.4%) swabs after 1 and 2 days of incubation, respectively, and the median reporting times for positive specimens were 33.7 hr (range, 18.2-156.9 hr) and 108.1 hr (range, 69.8-181.0 hr), respectively. Detection rate after 1-day incubation was 85%. Additional 1-day incubation improved detection rate; however, it prolonged the reporting times of positive specimens approximately up to 4 days because of the need for confirmatory tests such as species identification and susceptibility tests. Following a 2-day culture with ChromID MRSA, rapid confirmatory tests are warranted to reduce delay in identifying MRSA carriers.
Humans
;
Methicillin-Resistant Staphylococcus aureus/*isolation & purification
;
Nasal Cavity/microbiology
;
Reagent Kits, Diagnostic
;
Sensitivity and Specificity
;
Staphylococcal Infections/*diagnosis/microbiology
;
Time Factors
6.Investigation of intranasal bacteriological character and pH value in patients with chronic rhinitis treated by Budesonide aqueous nasal spray.
Wei-ping WEN ; Hui-wen ZHUANG ; Geng XU ; Jian-bo SHI ; Hong-yan JIANG ; Li-jing HU
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2005;40(12):917-921
OBJECTIVETo investigate the bacteriological character and pH value in nasal cavity and observe the efficacy following 8 week treatment with Budesonide aqueous nasal spray in chronic nonallergic rhinitis.
METHODSForty-two patients with chronic nonallergic rhinitis were treated with Budesonide aqueous nasal spray (256 microg once daily intranasal) for 8 weeks. Bacteriological character was measured before and 8 weeks after treatment All the swab specimens were obtained from the inferior meatus, bacteriologic outcome was determined by general cultures. Nasal pH value was measured by using a probe sited under the inferior turbinate before and after treatment. Efficacy was evaluated by measurement of nasal symptom scores and sign scores before and 8 weeks after treatment.
RESULTSThe bacterial growth was present in 37 of 42 cases (88%) before the treatment (group 1), including 53 aerobic bacteria. The bacterial growth was present in 38 of 42 cases (90.47%) 8 weeks after treatment (group 2), including 46 aerobic bacteria. There was no significant difference in the bacterial distribution between group 1 and group 2 (chi2 = 0.416, P > 0.05). Budesonide aqueous nasal spray reduced pH value from 7.90 +/- 0.39 to 7.70 +/- 0.23 (t = 2.72, P < 0.05). All the parameters of symptoms were improved after treatment, including nasal obstruction, nasal secretions, itchy feeling of the nose, closed rhinolalia and headache.
CONCLUSIONSGlucocorticoid aqueous nasal spray could be used safely for eight weeks and does not increase the risk of aerobes and fungi infection and reduce the pH in chronic nonallergic rhinitis. It is effective in relieving symptoms of patients with chronic nonallergic rhinitis.
Administration, Intranasal ; Adult ; Aged ; Budesonide ; administration & dosage ; therapeutic use ; Chronic Disease ; Female ; Humans ; Hydrogen-Ion Concentration ; Male ; Middle Aged ; Nasal Cavity ; microbiology ; Rhinitis ; drug therapy ; microbiology ; physiopathology
7.Infection of nasal cavity and facial tissue by Mycobacterium avium-intracellulare: report of a case.
Wen-mang XU ; Xia LI ; Yuan-yuan WANG ; Li-lin YANG ; Ju-lun YANG
Chinese Journal of Pathology 2012;41(4):281-282
Antigens, CD
;
metabolism
;
Antigens, Differentiation, Myelomonocytic
;
metabolism
;
Face
;
microbiology
;
Female
;
Humans
;
Middle Aged
;
Mycobacterium avium Complex
;
isolation & purification
;
Mycobacterium avium-intracellulare Infection
;
metabolism
;
microbiology
;
pathology
;
Nasal Cavity
;
microbiology
;
Nose Diseases
;
metabolism
;
microbiology
;
pathology
;
Vimentin
;
metabolism
8.Homology analysis of the epidemiological strains of meticillin-resistant Staphylococcus aureus and the strains isolated from the nasal fossa of the medical staff and inpatients.
Ya-li ZHANG ; Hong-wei ZHOU ; Li MA ; Qian WEN ; Yin-yin WANG ; Qiong LI ; Neng-ping WANG
Journal of Southern Medical University 2006;26(5):635-637
OBJECTIVETo investigate the relation between the epidemiological strains of meticillin-resistant Staphylococcus aureus (MRSA) and the strains isolated from the nasal fossa of the medical staff and inpatients.
METHODSThe MRSA strains were isolated from the nasal fossa of the medical staff and inpatients in the Department of Neurosurgery. The genes of the isolated strains were amplified by randomly amplified polymorphic DNA (RAPD) assay.
RESULTSThree and 12 MRSA strains were isolated from the nasal fossa of the medical staff and patients who were hospitalized for more than 1 week, respectively, and RAPD assay revealed high homology between the isolated strains.
CONCLUSIONCross infection can be present between the medical staff, inpatients, and the infected patients.
China ; epidemiology ; Cross Infection ; microbiology ; DNA, Bacterial ; genetics ; isolation & purification ; Humans ; Infectious Disease Transmission, Professional-to-Patient ; Inpatients ; Medical Staff ; Methicillin Resistance ; Nasal Cavity ; microbiology ; Phylogeny ; Random Amplified Polymorphic DNA Technique ; Staphylococcal Infections ; epidemiology ; microbiology ; Staphylococcus aureus ; classification ; genetics ; isolation & purification
9.Bacteriological analysis of persistent rhinosinusitis after endoscopic sinus surgery.
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2012;26(8):351-353
OBJECTIVE:
To investigate the bacterial characteristics of persistent rhinosinusitis after functional endoscopic sinus surgery (FESS).
METHOD:
Twenty patients with nasal septum deviation, 30 patients with chronic rhinosinusitis (CRS) and 20 patients with persistent rhinosinusitis, were selected to take discharges from middle meatus during the operation. Bacteria culture and drug susceptibility of the discharges were compared between three groups.
RESULT:
There were 13, 15 and 15 isolates detected in nasal septum deviation group, CRS group and persistent rhinosinusitis group. There was no significant difference among the three groups at the detection rate of Gram-positive bacteria. But there was significant difference between the persistent rhinosinusitis group and the other two groups at the detection rate of Gram-negative bacteria. The detection rate of antibiotic-resistant bacteria were significantly higher in persistent rhinosinusitis group than in CRS group.
CONCLUSION
Aerobic bacteria can live in nasal cavity. Bacteria infection is one of the etiological factors of persistent rhinosinusitis after FESS. Gram-negative bacteria and antibiotic resistant bacteria are increased in patients with persistent rhinosinusitis. To treat the persistent rhinosinusitis after surgery, the antibiotics should be reasonably used according to the bacteria culture and the drug susceptibility.
Adolescent
;
Adult
;
Aged
;
Bacteria, Aerobic
;
isolation & purification
;
Bacterial Infections
;
microbiology
;
Chronic Disease
;
Disease Susceptibility
;
Endoscopy
;
Female
;
Humans
;
Male
;
Middle Aged
;
Nasal Cavity
;
microbiology
;
Postoperative Period
;
Sinusitis
;
microbiology
;
surgery
;
Young Adult
10.Preliminary study on the aerobes distribution of nasal cavity from the healthy children and adults.
Yanli CHEN ; Xiaoling XU ; Yulan CHEN ; Jingyun FU ; Jing ZHANG ; Xiaomei WU ; Xuefang LIU ; Hui ZHANG ; Bo SUN
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2007;21(23):1068-1069
OBJECTIVE:
To study whether there are differences in the bacteria distribution from the nasal cavity of the healthy children, teenagers and adults
METHOD:
The cotton swab specimens were taken from the nasal cavity of the healthy children, teenagers and adults for aerobic culture training.
RESULT:
Corynebacterium, coagulase-negative staphylococci, Staphylococcus aureus, Gram-negative Neisseria, alpha-hemolytic streptococcus and gram-negative bacillus with, six species of bacteria in total, were cultivated from the nasal cavity of 40 healthy children. The positive rate of bacterial culture was 80.0%; Corynebacterium, coagulase-negative staphylococci and Staphylococcus aureus were cultivated from the nasal cavity of 40 healthy teenagers and 56 healthy adults with the positive rate of bacterial culture was 90.0% from the healthy teenagers group and was 92.9% from the healthy adults group.
CONCLUSION
There were obvious difference between the nasal cavity of the healthy children and adults with no obvious difference between the bacteria distribution from the nasal cavity of healthy teenagers and adults.
Adolescent
;
Adult
;
Child
;
Female
;
Gram-Negative Bacteria
;
isolation & purification
;
Gram-Positive Bacteria
;
isolation & purification
;
Humans
;
Male
;
Middle Aged
;
Nasal Cavity
;
microbiology
;
Young Adult