1. Anti-hemolytic, antibacterial and anti-cancer activities of methanolic extracts from leaves and stems of Polygonum odoratum
Nittaya CHANSIW ; Kamonnaree CHOTINANTAKUL ; Narisara PARADEE ; Somdet SRICHAIRATTANAKOOL
Asian Pacific Journal of Tropical Biomedicine 2018;8(12):580-585
Objective: To investigate anti-hemolytic, antibacterial and anti-cancer activities of leaf and stem extracts from Polygonum odoratum. Methods: Leaves and stems of Polygonum odoratum were extracted using methanol and their anti-hemolytic activity was assessed using 2, 2′-Azobis (2-methylpropionamidine) dihydrochloride which is known to generate free radical damage on cell membranes of red blood cells. This damage, represented by hemolysis, was measured using spectrophotometry. Antibacterial activity was tested by using a broth microdilution method to find minimal inhibitory concentrations against eight bacterial strains. Anti-cancer activity of the extracts was evaluated against a human promyelocytic leukemic cell line (HL-60) by using MTT assay for cell viability and flow cytometry for apoptosis induction and cell cycle analysis. Results: Both leaf and stem extracts have anti-hemolytic activity. The results showed a significantly increased percentage of inhibition in a concentration-dependent manner. Interestingly, the leaf extract showed anti-hemolytic activity to a greater extent than the stem extract. Antibacterial activity of the extracts, as indicated by their minimal inhibitory concentration, using 12.5, 50, 25, 25 μg/mL, was measured against Staphylococcus epidermidis, Enterococcus faecium, Enterococcus faecalis and Staphylococcus aureus. The leaf extracts also exhibited anti-cancer activity, demonstrated by significantly decreased cell viability of human promyelocytic cells (HL-60), with an IC
2. Thai Perilla frutescens fruit oil alleviates carbon tetrachloride-induced hepatotoxicities in rats
Narisara PARADEE ; Adchara PROMMABAN ; Pimpisid KOONYOSYING ; Somdet SRICHAIRATANAKOOL ; Duangta KANJANAPOTHI ; Tawat TAESOTIKUL ; Sarawut KONGKARNKA
Asian Pacific Journal of Tropical Biomedicine 2020;10(3):101-110
Objective: To study the effect of perilla fruit oil against carbon tetrachloride (CCl