Background. This study is to determine mode of metabolism on triple collaboration bridges of traditional medicine, modern medicine and NCM.Goal. To determine membrane redoxy potentials three line involves important regulation factors on mode of metabolism which relationship connected with rlung, mkris, and badgan symbolic code.Materials and Methods. Only 81 healthy individuals were involved in the study. Proton leak was determined by quantity rate of MDA in cell membrane and membrane resistance, proton conductance was determined by serum and urine oxidase activity.Results. The table 1 shows quantity rate of membrane resistance was decreased 1.08-1.52 fold, HDL content was decreased, and however LDL was increased. This result is to manifest low proton leak which means this type is likely belonged to badgan symbolic code with qualities cold fatty, earth, water. The table 2 shows serum and urine oxidize activity 2.22-6.1 fold was increased, HDL content was increased; UCP-3 gene activity relatively was increased. This result is to manifest highproton conductance which means this type is likely belonged to mkris symbolic code with qualities hot fatty, fire.Conclusions:1. Individuals with high proton leak and slow proton conductance had serum and urine oxidize activity were weak, therefore there are visceral and subcutaneous fat were low.2. Individuals with medium proton leak and high proton conductance had serum and urine oxidize activity were high, therefore there are visceral was low and subcutaneous fat was high.3. Individuals with weak proton leak and medium proton conductance had serum and urine oxidize activity were medium, therefore there are visceral was high and subcutaneous fat was low.

The pathogenesis of chronic obstructive pulmonary disease (COPD) encompasses a number of injurious processes, including an abnormal inflammatory response in the lungs to inhaled particles and gases. Other processes, such as failure to resolve inflammation, abnormal cell repair, apoptosis, abnormal cellular maintenance programs, extracellular matrix destruction (protease/antiprotease imbalance), and oxidative stress (oxidant/antioxidant imbalance) also have a role. The inflammatory responses to the inhalation of active and passive tobacco smoke and urban and rural air pollution are modified by genetic and epigenetic factors. The subsequent chronic inflammatory responses lead to mucus hypersecretion, airway remodeling, and alveolar destruction. This article provides an update on the cellular and molecular mechanisms of these processes in the pathogenesis of COPD. During the past decade a plethora of studies have unravelled the multiple roles of nitric oxide (NO) in airway physiology and pathophysiology. In the respiratory tract, NO is produced by a wide variety of cell types and is generated via oxidation ofL-arginine that is catalyzed by the enzyme NO synthase (NOS). NOS exist in three distinct are forms: neuronal NOS (nNOS), inducible NOS (iNOS), and endothelial NOS (eNOS). NO derived from the constitutive are forms of NOS (nNOS and eNOS) and other NO-adduct molecules (nitrosothiols) have been shown to be modulators of bronchomotor tone. On the other hand, NO derived from iNOS seems to be a proinflammatory mediator with immunomodulatory effects. Finally, the production of NO under oxidative stress conditions secondarily generates strong oxidizing agents (reactive nitrogen species) that may modulate the development of chronic inflammatory airway diseases and/or amplify the inflammatory response.

Background:In females reproductive system aging is very important. Females spend the last 1/3 period of their life in insufficiency of sex hormone of late menopause. Due to decrease of ovary function noticeable change is detected in nervous endocrine system. Although menstrual cycle hasn’t been lost amount of FSH increased. It shows decrease of reproductive function females. Therefore recently researchers define that amount of FSH increase is an important biomarker which detects in an early period of menopause. But amount of estradiol hormone is almost in normal level until the late post menopause. Goal: to study the dynamic feature of age related changes and dependence of serum FSH and estradiol level in female aging.Materials and Methods:In this study were involved 177 healthy Mongolian women aged above 35 years old. We drained 5 ml fasting vein blood at 8.00-10.00 am. Sera were separated and kept frozen until assayed by ELISA. Results:Аaverage mean of FSH was 19.84±22.6 IU/l, at the age of 35-45 1.62±3.29 IU/l, at the age of 46-55 16.39±15.39 IU/l, at the age of 56-65 31.38±33.69IU/l, at the age of 66-75 28.83±17.31 IU/l, over 75 34.52±13.94 IU/l and there was positive correlation between age and FSH levels (r=0.647, p<0.001).Average mean of estradiol of participants: at the age of 35-45 37.03±22.09 pg/ml, at the age of 46-55 21.1±16.65pg/ml, at the age of 56-65 13.7±20.07 pg/ml, at the age of 66-75 9.11±15.1pg/ml, over 75 age 1.8±1.53 pg/ml and there was inverse correlation between age and estradiol levels (r= -0.453, р<0.001).There was an inverse correlation between log E2 and FSH levels (r= -0.434, p<0.001). Multiple regression analysis shows that age (β= -0.350, p<0.001) and FSH (β=-0.222, p=0.016) had significant inverse correlation with serum estradiol level. Conclusion: For females over 35 average mean of FSH is 19.84±2.26 IU/l, average mean of estradiol hormone is 19.6±2.0pg/ml. between the mid age (35–45 years) and oldest (>75 years) groups, mean estradiol level declined by 20.6 times, whereas FSH increased by 21.3 times. It shows that this is an important marker of menopause. Multiple regression analysis shows that age (β= -0.350, p<0.001) and FSH (β= -0.222, p=0.016) had had significant inverse correlation with serum estradiol level.

INTRODUCTION: Gonadotropins are released under the control of gonadotropin-releasing hormone (GnRH) from thearcuate nucleus and preoptic area of the hypothalamus. The gonads — testes and ovaries — are the primary targetorgans for LH and FSH. The gonadotropins affect multiple cell types and elicit multiple responses from the targetorgans. As a simplified generalization, LH stimulates the Leydig cells of the testes and the theca cells of the ovaries to produce testosterone (and indirectly estradiol), while FSH stimulates the spermatogenic tissue of the testes andthe granulosa cells of ovarian follicles.Reproductive aging is endocrinologically characterized by a progressive rise in serum FSH levels associated with adecrease in serum estradiol (E2) and testosterone (T) levels. The rise in FSH is associated with reduced levels of sexsteroid and peptide negative feedback regulators of FSH secretion.The aim of study is: determination of serum FSH level changes in relation to aging and sex.MATERIALS AND METHODS: In this study were involved 169 healthy Mongolian adults aged above 45 years old. Subjectswere randomly selected and undergone physical examination by geriatrician. People, who are receiving hormonereplacement therapy, using inproper use of alcohol, injured and had surgery were excluded from our surgery. Bloodsamples were collected in the early morning (8.30–10.30 AM) after an all night fast and plasma was separatedimmediately by centrifugation; then sera obtained were stored at -20°C until assayed by ELISA kit from United BiotechCoLTD, USA, which sensitivity is 1mIU/ml. Statistical analyses have been performed by statistical software SPSS 16,using ANOVA, Pearson correlation, T-test.RESULT: Mean level of FSH for both sexes was 21.19±16.2 mIU/ml, which is in comparison with males (12.33±10.58mIU/ml) it was comparatively higher (p<0.001) in women (29.61±16.15 mIU/ml). FSH has no correlation with aging inman (r=0.084, p>0.05), but in women it was stronger correlation (r=0.203, p<0.05). in 51-60 years age group FSH wasincreased by 56%, in 61-65 years group by 91%, in 66-70 years group it was increased 100% in comparison with until50 years age group. In older age (above 70 years) it decreased to 70% from reached concentration. ANOVA analysishas not showed significant difference between age groups.CONCLUSIONS: Average mean of FSH in old age are: 29.61±16.15 mIU/ml in women and 12.33±10.58 mIU/ml in men.Correlation with aging was observed stronger in women than in man (r=0.203, p<0.05). FSH increases with aging untilround 70 and decreases after 70 years old.

Rotenone is a specific inhibitor of the NADH dehydrogenase complex. In mitochondria, rotenone inhibitsthe oxidation of NADH to NAD, thereby blocking the oxidation of NAD and the substrates such asglutamate, alpha-ketoglutarate, and pyruvate. Rotenone also inhibits the mitochondrial respiratory chainbetween diphosphopyridine nucleotide and flavine.2, 4-Dinitrophenol – (DNP) is lipophilic weak acids that pick up a proton, transport across the mitochondrialinner membrane into the matrix, deprotonate, then exit as anions before repeating the catalytic cycle,and dissipating the proton gradient. In this situation, electrons continue to pass through the electrontransport system, reduce oxygen to water and metabolic rate, heat are increased, but ATP is lesssynthesized in this process.The macrolide antibiotic - oligomycin binds to the surface of the c8-10 ring of the Fo domain of ATPsynthase, making contact with two neighboring molecules and blocking proton flow, which explains theinhibitory effect on ATP synthesis. Intraperitoneal injection of oligomycin into the rat (0.5 mg per kg)reduces the oxygen consumption by about 50%; decreases ATP production by the aerobic pathway andincreases formation of lactate in blood serum. These changes may cause a decelerated metabolism andan increased formation of free radicals or ROS in membranes.