In order to find out an index for the assessment of chromium deficiency in diabetic patients, the contents of chromium in the hair of twenty-seven diabetic patients and sixty-three healthy subjects were determined. The contents of chromium in hair was determined with a flameless atomic absorption technique. The mean recovery rate was 103% and the CV was 4.3%.The results showed that the mean value of diabetic patients (104.22?61.99ng/g) was significantly lower than that of healthy subjects (173.19?79.96ng/g) (p