Objective: To observe the clinical efficacy in lowering blood lipids with ultrasound therapy device and Xuezhikang. Methods:Selected 80 cases as an object of study in Heilongjiang Provincial Hospital Integrative Medicine treated from January 2013 to July 2014, the observation group 40 cases, used ultrasound therapy device and Xuezhikang, control group 40 cases, only single use of Xuezhikang overall treatment, compared the two groups of patients before and after treatment and lipid levels in the blood. Results:The total efficiency of compared two groups of patients, a statistically significant (x2=13.536, P<0.05);compare TG group before and after treatment in the observation group, TC, HDL-C, LDL-C, were statistically significant(t=3.265, t=4.021, t=-2.871, t=3.665;P<0.05). In the control group before and after treatment group TG, TC, LDL-C were statistically significant (t=2.18, t=2.994, t=2.276;P<0.05). After treatment, the comparison between the two groups TG, TC, HDL-C, LDL-C, were statistically significant (t=-2.998, t=-3.046, t=2.887, t=3.028;P<0.05). Conclusion:Combined use of ultrasound therapy device and Xuezhikang lipid-lowering therapy, can significantly lower blood lipid levels in patients, and the effect is significant, worthy of clinical application.

ObjectiveTo explore the relationship of adult attachment,coping style and job burnout among nurses.MethodsRelationship Questionnaire(RQ) and Experience of close relationships (ECR) inventory wereused to measure 360 nurses'adult attachment.Coping style scale and Maslach's Burnout Inventory-General Scale were used to measure subjects'coping strategies and job burnout.Results ①Distribution of adult attachment style in nurses were:103 for secure,86 for dismissing,67 for preoccupied and 21 for fearful.② There was significant positive correlation between attachment avoidance and denying the problem and mental disengagement ( r =0.136) ;attachrnent anxiety was positively related to deuying the problem and mental disengagement( r =0.386),and to avoiding the problems and shifting attention( r =0.347 ).③ Attachment avoidance was significantly related to depersonalization ( r =0.147 ) ; there were significant positive correlations between attachment anxiety and emotion exhaustion( r=0.362) and depersonalization ( r =0.390).④ There were no significant differences amongfour attachment groups on seeking emotional support and venting of emotions,avoiding the problems and shifting attention,and emotion exhaustion,ineffectiveness.⑤Regression analysis suggested that emotion exhaustion can be predicted by attachment anxiety and denying the problem and mental disengagement,while iueffectiveness only can be predicted by active coping focused on the problens.ConclusionBoth adult attachment and coping style are contributed to job burnout among nurses.

Objective To observe the effect of several residual adhesive methods on the enamel surface ,and conduct lab evalua-tion .Methods Sixty premolars extracted because of orthodontic treatment .And all the teeth were randomly divided into 3 groups . Group 1:tungsten carbide burs + silicon particles ;Group 2 :ultrasonic scaling + silicon particles ;Group 3:silicon particles ,each with 20 premolars .After underwent several methods ,the surface roughness differences ,operation time were determined and ob-served with the scanning electron microscope .And the result was statistically analyzed .Results There were significant differences in the surface roughness and operation time among the three groups (P<0 .05) ,The scanning electron microscope after polishing showed that the teeth surface had different degrees of injury ,the silica particles group had less superficial scratch .Conclusion The tungsten carbide burs and ultrasonic instrument for debonding before the silica particles had less superficial scratch .

Lycopus lucidus is a herbal drug that can invigorate blood circulation,resolve blood stasis and improve microcirculation. In our further study of its pharmacological function and to identify its effective components,its effect on the hemorrheology of rat was used as a guide to evaluate the relative strength of four different extraction fractions of the drug. Results showed that fraction L. F04 and L. F02 were effective in reducing the blood viscosity,with L. Fo4 showing the best effect. This function of L. F04 was believed to be related to its ability to inhibit platelet aggregation and to improve the compostion of plasma

Objective To analyse the effects of high insulin on the expression and function of P-glycoprotein (P-gp), and preliminarily investigate the influence of insulin on chemotherapeutic sensitivity in MCF-7/ADR cells. Methods MCF-7/ADR cells were cultured with different concentrations of insulin(0.001, 0.005, 0.01, 0.05 and 0.1μmol/L). Real-time PCR was used to detect the expression of P-gp mRNA. Western blot assay was used to detect the expression level of P-gp. Rhodamine 123 was used to detect the efflux function level of P-gp. Cell viability and chemotherapeutic sensitivity were detected by MTT assay. Results High concentration of insulin (0.1 μmol/L) promoted the proliferation of MCF-7/ADR cells. The concentration of insulin (0.05 and 0.1 μmol/L) accelerated P-gp mRNA and protein expression, which also augmented the efflux function of P-glycoprotein and reduced the chemotherapeutic sensitivity to epirubicin. Conclusion High concentration of insulin may influence the drug resistance of breast cancer cells by promoting the expression and function of P-glycoprotein of MCF-7/ADR cells.

Objective:To investigate the role of Leukotactin-1(LKN-1),CC receptor 1(CCR1)and CC receptor 3(CCR3)in the pathogenesis of hypertensive disorder complicating pregnancy(HDCP),the expressions of these factors in placentas were measured.Methods:The levels of LKN-1,CCR1 and CCR3 in placentas were measured from 32 patients with HDCP(study group)and 32 normal pregnant women(control group)by western blot(WB).The localization of these factorsin placentas were detected by immunohistochemistry(IHC).Results:LKN-1 protein expression was negative in placentas from the two groups by WB and IHC,while both CCR1 and CCR3 in the HDCP group were significantly lower than those in the control group(CCR1 0.66±0.06 vs 0.88±0.03.CCR3 0.17±0.01 vs 0.27±0.01,P<0.01).CCR1 was localized in vascular endothelial cells and stroma cells in both groups,but rare in the trophoblast layer.CCR3 was localized in vascular endothelial cells and trophoblast cells in both groups.Conclusions:There is no LKN-1expression in placentas from the two groups.Down regulation of CCR1 and CCR3 may associate with the pathogenesis of HDCP.

Objective To observe the effect of exogenous insulin on the expression of P-glycoprotein (P-gp)and the secretion of insulin in pancreatic beta cells (INS-1 832/13).Methods Insulinoma cells (INS-1 832/13) were cultured with 0.5 μmol/L exogenous insulin for 30 days.MTT assay was used to measure cell viability.Quantitative RT-PCR and western blot were used to detect the expression of P-gp mRNA and protein respectively,and glucose stimulated insulin secretion (GSIS) were measured by radioimmunoassay.Results Compared with control group,0.5 μmol/L exogenous insulin promoted the viability of INS-1 832/13 cells [(102.00±12.99) vs (356.00±35.51),P<0.05] and accelerated P-gp expression [(107.50±17.08) vs (307.50±44.25)] both at mRNA and protein levels [(105.00±12.91) vs (192.50±35.94),P<0.05].Glucose stimulated insulin secretion was positively correlated with P-gp expression level,but had no significant effect on basal insulin secretion.Conclusion Exogenous insulin can promote the secretion function of INS-1 832/13 cells,and the mechanism may be related to the expression of P-gp.

Objective : To examine the association between intrinsic capacity and falls in older adults, so as to provide insights into the risk assessment of falls. Methods: Older adults aged 60 years and above were selected from two districts and one county in Bengbu City, Anhui Province from September 2022 to June 2023 using convenience sampling method. Demographic information, health-related behaviors and incidence of falls among participants were collected through questionnaire surveys. The intrinsic capacity included five dimensions: sensory, motor, vitality, cognition and psychology, which were investigated by the sensory dimension screening scale recommended by the World Health Organization, the Simple Physical Functioning Battery (SPPB), the Micro Nutritional Assessment Scale (MNAS-SF), the Brief Intelligent Mental State Examination Scale (MMSE), and the Center for Evaluation of Streamlined Depression Levels 10-entry scale (CESD-10), respectively. A total score of 1 or more indicated a decrease in intrinsic capacity. The association between intrinsic capacity and falls in older adults was analyzed by a multivariable logistic regression model. Results: A total of 1 950 questionnaires were allocated, and 1 917 were valid, with an effective rate of 98.30%. There were 934 men (48.72%) and 983 women (51.28%), with a mean age of (68.15±3.42) years. There were 1 352 rural residents (70.53%) and 1 431 illiterate and primary school-educated residents (74.65%). In the past year, 347 residents fell, accounting for 18.10%. The median comprehensive score for intrinsic capacity was 1.00 (interquartile range, 2.00) points, and 1 320 had a decrease in intrinsic capacity, accounting for 68.86%. Multivariable logistic regression analysis showed that decline in intrinsic ability was associated with the risk of falls after adjustment for age, gender, educational level, marital status, alcohol consumption and self-rated health status (OR=1.531, 95%CI: 1.408-1.721). Conclusion Decreased intrinsic capacity in older adults may contribute to an increased risk of falls.

Objective To explore the relationship of adult attachment,life orientation style and job burnout among nurses.Methods Relationship Questionnaire (RQ) and Experience of close relationships (ECR) inventory were used to measure 566 nurses' adult attachment.Life orientation test (LOT-R) and Maslach's Burnout Inventory-General Scale (MBI-GS) were administrated to measure subjects'optimism,pessimism,and job burnout.Results ①Distribution of adult attachment style in nurses were 36％ for secure,29.3％ for dismissing,18.4％for preoccupied and 6.7％ for fearful.②Attachment avoidance were positive correlated with pessimism (r =0.139) and negative correlated with optimism (r =-0.212) significantly; and there was significant positive correlation between attachment avoidance and ineffectiveness (r=0.122) also.Attachment anxiety was positively related to pessimism (r=0.151),and three indexes of job burnout (r=0.238,0.281,0.143 respectively).Meanwhile,there were significant negative correlations among optimism and job burnout indexes (r =-0.12,-0.193,-0.230).③There were significant differences among four attachment groups on optimism and pessimism.For job burnout,the four groups had significant differences only on depersonalization,instead of ineffectiveness and emotion exhaustion.④ Regression analysis suggested that attachment anxious was a valid positive predictor of job burnout,while optimism was a negative predictor.Conclusion Both adult attachment and life orientation style are contributed to job burnout among nurses.

Background Optic neuritis is one of the common clinical neuro-ophthalmic diseases.Spectraldomain OCT (SD-OCT) is a valuable tool in assessing the thickness changes of retina,while enhanced depth imaging (EDI) OCT can further quantitatively and morphologically evaluate the changes of retina and choroid.The pathological mechanism of optic neuritis is unclear now.Objective This study was to quantitatively measure the retinal and choroidal thickness in early optic neuritis eyes by SD-OCT and EDI OCT.Methods A prospective cohort study was carried out in Tianjin Eye Hospital from July 2015 to May 2016.Twenty eyes of 20 patients with acute optic neuritis were enrolled as optic neuritis group and 22 eyes of 20 healthy subjects with matched age and gender were included in the normal control group.The mean thickness of retinal nerve fiber layer (RNFL) and choriod in superior,inferior,nasal and temporal quadrants at 3.4 mm around optic disc was measured,and the mean thickness of RNFL,ganglion cell layer (GCL),inner plexiform layer (IPL),inner nuclear layer (INL),outer plexiform layer (OPL),outer nuclear layer (ONL) and photoreceptor layer also was measured by EDI OCT.Pattern visual evoked potential (P-VEP) and visual field were examined in all the individuals,the correlations of mean defect (MD) with the thickness of RNFL,choroid and the thickness of RNFL,GCL,IPL,INL,OPL,ONL,photoreceptor layer at macular area were evaluated.Results The RNFL thickness values were (424.00±160.30),(428.40±169.83) and (108.15 ±50.66) μm in superior,inferior,nasal quadrants at 3.4 mm arear around optic disc in the optic neuritis group,which were significantly higher than (265.68±26.25),(283.27±52.81) and (72.68± 12.01) μm in the normal control group (t=4.571,3.814,3.190,all at P＜0.01),and there was no significant difference between the two groups (t =0.849,P =0.401),and no significant differences were found in choroidal thickness of 4 quadrants between the two groups (all at P＞0.05).The thickness of RNFL,GCL and IPL at 1 mm area around macula and the thickness of GCL,IPL,INL at 3 mm area around macula were evidently thining in the optic neuritis group compared with the normal control group (all at P＜0.05).The latency of P100 wave was (133.15±11.11) seconds in the optic neuritis group and that in the control group was (94.59 ±4.38) seconds,showing a significnat difference between them (t =15.058,P＜0.05).Positive correlations were found between MD and the RNFL thickess in superior,inferior,nasal quadrants at 3.4 mm arear around optic disc (r =0.649,0.649,0.635,all at P＜0.05),however,no evidentlylinear correlations were found between MD and choroidal thickness in 4 quadrands (r =-0.120,-0.102,-0.415,0.120,all at P＞0.05),and the thickness of RNFL,GCL,IPL,INL,OPL,ONL,photoreceptor layer at macular area was significantly linear correlated with MD.Conclusions EDI OCT can reflect the RNFL edema around optic disc and thining of various layers of retina at macular area in acuter optic neuritis eyes,however,the choroidal thickess is unchanged.EDI OCT is a useful tool in quantitative evaluation of retinal and choroidal thickness of early optic neuritis.