MicroRNAs (miRNAs) are a class of highly conserved single-stranded RNA molecules that modulate gene translation. By targeting the mRNA of protein- coding genes, miRNAs play a critical role in neuronal differentiation, cell proliferation, apoptosis and metabolism. There are a lot of miRNAs in central nervous system, which are not only closely linked to development, differentiation and function of nerve cells, also play an important role in nerve lesions and dysfunction after cerebral ischemia. Specific miRNA through their own changes affect their target gene expression levels after focal cerebral ischemia, involving in the protection against apoptosis in neurons and regeneration after cerebral ischemia. A full understanding of the specific microRNA function and its underlying mechanism in the brain can provide a new strategy for the prevention, diagnosis, and treatment of ischemic cerebral injury at gene level.
Animals ; Brain Ischemia ; Humans ; MicroRNAs

Reflecting on the historical responsibility of Chinese medical ethics at present， it is necessary to place oneself in the grand context of promoting the process of building a community with a shared future for mankind. Safety， development， and civilization constitute the three important ethical dimensions of a community with a shared future for mankind， which are not only ethical orientations， but also ethical appeals. These provide an important direction for Chinese medical ethics to deeply consider its role in the process of a community with a shared future for mankind. The historical responsibilities of Chinese medical ethics in the process of promoting a community with a shared future for mankind mainly include： confronting moral and interest issues， adhering to the patient-centered approach， and ensuring patients’ life safety； playing the role of ethical regulation， achieving the practical effects of medical ethics， and maintaining social security and stability； implementing the responsibility of medical ethics education， shaping medical professionalism， and promoting the development of healthy China； inheriting the excellent traditional culture， pursuing medical excellence and justice， and promoting the development of social morality； pursuing common health values， building a community with a shared future between doctors and patients， and promoting the progress of human. A global community of health for all provides the most direct practical platform for Chinese medical ethics to fulfill its historical responsibility in the process of building a community with a shared future for mankind. It directly promotes Chinese medical ethics to the world， and Chinese ethics facing the world will play an increasingly important influence in self-renewal.

OBJECTIVE: To investigate factors that affected the distribution of rehydrated particle size of freeze-dried docetaxel liposome and develop a technique of producing docetaxel liposome that can meet clinical requirements. METHODS: The single factor method was utilized in this study. Docetaxel liposome were prepared using different types of negatively charged phospholipids, or lecithins with varying phosphatidylcholine (PC) content, or various cryoprotectants at different gradient concentrations, followed by freeze-drying with different protocols. The particle sizes of corresponding rehydrated products were compared and the effects of different factors were analyzed. RESULTS: The optimal liposomal membrane was composed of dipalmitoyl phosphatidic acid (DPPA), 20% trehalose and lecithin containing 92% PC. The best freeze-drying protocol included following steps; flash freezing, 19 h of sublimation-drying and 9 h of desorption-drying. CONCLUSION: The parameters, including negatively charged phospholipids, cryoprotectants, PC content of lecithin and lyophilization protocols, had various effects on the particle sizes of reconstituted docetaxel liposome.

To study the effect of glucagon-likepeptide 1(GLP-1)receptor agonist on insulin resistance and hepatic oxidative stress in rats with diabetes mellitus combined with nonalcoholic fatty liver disease. 36 male SD rats were served as the experimental animal and randomly divided into control group, model group, and GLP-1 group. The rats of control group were given routine diet with intraperitoneal injection of normal saline, those in model group were given high fat diet and intraperitoneal injection of normal saline, while GLP-1 group rats were fed with high fat diet and intraperitoneal injection of liraglutide. After 4 weeks of treatment, insulin resistance, lipid metabolism, liver injury and oxidative stress were all assessed. Serum fasting blood glucose, fasting insulin, total cholesterol, triglyceride, alanine transaminase(ALT), aspartate transaminase(AST)levels and total cholesterol, triglyceride contents in liver tissue, and as well as homeostasis model assessment for insulin resistance(HOMA-IR)levels of model group were significantly higher than those of control group, complex insulin sensitivity index(ISIcomp)level was significantly lower than that of control group; serum fasting blood glucose, fasting insulin, total cholesterol, triglyceride, ALT, AST contents and HOMA-IR levels of GLP-1 group were significantly lower than those of model group, ISIcomp level was significantly higher than that of model group; superoxide dismutase(SOD), glutathione peroxidase(GSH-Px), catalase(CAT)contents in liver tissue of model group were significantly lower, while malondialdehyde content and SOD, GSH-Px, CAT, NF-E2 related factor-2(Nrf-2), antioxidant response element(ARE), heme oxygenase-1(HO-1), quinone oxidoreductase-1(NQO-1), glutathione thiol transferase(GST)mRNA expression were significantly higher than control group; SOD, GSH-Px, CAT contents and SOD, GSH-Px, CAT, Nrf-2, ARE, HO-1, NQO-1, GST mRNA expression in the liver tissue of GLP-1 group were significantly higher, while malondialdehyde content was significantly lower than that of model group. GLP-1 receptor agonist reduces insulin resistance and liver oxidative stress injury in diabetic rats with nonalcoholic liver disease.

OBJECTIVETo explore the treatment of casualties who suffered from multiple injuries in earthquake and had been treated by damage control (DC) immediately,and the definite postponed surgery time by way of APACHE II score.

METHODSFrom May to June 2008, 21 casualties who had undergone DC accepted further treatment of medicine and surgery, and recovered physical potentiality in the intensive care unit (ICU). APACHE II score was adopted to evaluate the casualties' physical potentiality. Then, internal fixation of intra-articular fractures and centrum fractures, alternation from external fixation to internal fixation, reconstruction of soft tissue was applied by stages according to the plan.

RESULTSAll the patients were effectively cured after definite postponed surgery. There was less complication, low deformity ratio and no death happened.

CONCLUSIONTimely evaluation of the casualties' physical potentiality, appropriate surgery time and exact surgery can reduce the casualties' complication, deformity rate and death rate.

Adolescent ; Adult ; Aged ; Aged, 80 and over ; China ; Earthquakes ; Female ; Fracture Fixation ; Fractures, Bone ; complications ; surgery ; Humans ; Intensive Care Units ; Male ; Middle Aged ; Multiple Trauma ; complications ; surgery ; Treatment Outcome

After the articular cartilage injury, the metabolic level is increased during the progressive degeneration, the chondrocytes secrete a variety of inflammatory factors, and the original cell phenotype is gradually changed. For a long time, a large number of researchers have done a lot of researches to promote anabolism of chondrocytes and to maintain the stability of chondrocyte phenotype. There are many molecular signaling pathways involved in the process of promoting cartilage repair. This review focuses on the key signaling molecules in articular cartilage repair, such as transforming growth factor-beta and bone morphogenetic protein, and reveals their roles in the process of cartilage injury and repair, so that researchers in related fields can understand the molecular mechanism of cartilage injury and repair widely and deeply. Based on this, they may find promising targets and biological methods for the treatment of cartilage injury.
Bone Morphogenetic Proteins ; physiology ; Cartilage, Articular ; growth & development ; injuries ; Chondrocytes ; physiology ; Humans ; Regeneration ; Signal Transduction ; Transforming Growth Factor beta ; physiology

OBJECTIVETo study pre-treatment in determining total polysaccharide in flos Chrysanthemi Indici.

METHODThe factors including the extraction temperature, extraction time, ratio of material to liquid were studied. The best extraction condition was found through the response surface design.

RESULTThe best extraction condition as follows: 81.0 degrees C of the extraction temperature, 1.6 h of extraction time, and the ratio of material to water as 1: 29. On these conditions the extraction rate of flos Chrysanthemi Indici was the best.

CONCLUSIONA model equation that can be used to predict the experiment is established through the response surface method.

Analytic Sample Preparation Methods ; Chrysanthemum ; chemistry ; Flowers ; chemistry ; Plant Extracts ; chemistry ; Polysaccharides ; analysis

OBJECTIVETo investigate the effect of light intensity on growth and photosynthetic of Chrysanthemum morifolium.

METHODThe growth characteristics of C. morifolium were measured under different treatments (100%, 80%, 60%, 40% and 20% of full sunlight). The photosynthetic characteristics and chlorophyll fluorescence parameters of leaves under different light intensity were determined by a LI-6400 photosynthesis system and a PAM-2100 chlorophyll fluorescence system.

RESULTWith the reduction of irradiance, the diameter of the stem reduced, plant height, leaf length, leaf width and length/width raised, assimilation product increased; Content of photosynthetic pigment increased between light intensity 100%-40% reduced under 20% treatment, chlorophyll a/b decreased. Light compensation point (LCP), apparent quantum yield (AQY) increased first and reduced later, photosynthesis rate (P(n)), stomatal conductance (G(s)), intercellular CO2 concentration (C(i)) and transpiration rate (T(r)) decreased, stomatal limitation value (L(s)) rose. Chlorophyll fluorescence parameters F(v)/F(m) increased, phiPS II, F(v)'/F(m)', ETR and qP increased between irradiance 100%-60%, NPQ decreased, such as phiPS II decreased and NPQ increased when irradiance was lower than 40%.

CONCLUSIONWeak light condition was unfavorable to the growth of C. morifolium and the light conditions of culture should be control between 80%-60% of full sunlight.

Biomass ; Chrysanthemum ; growth & development ; physiology ; radiation effects ; Photosynthesis ; radiation effects

OBJECTIVETo develop microsatellite primers with the method of isolation of genomic microsatellite in Pinellia ternata by magnesphere.

METHODBy taking advantage of the high binding affinity of biotin to streptavidin, microsatellite probe of the 5' end biotin was combined with magnesphere paramagnetic particles, and then combinations were hybridized with the digested P. ternata DNA fragments in which both ends of them connected with special adaptor, the other DNA fragments were eluted out. The microsatellite library was established. The crossed fragments were used as the template to conduct PCR amplification with the adapter sequences as primers, the products were cloned directly, subsequently screened by bacterium liquid PCR, and DNA sequencing was carried out.

RESULTFifteen microsatellites of P. ternata were obtained, development efficiency was 93.75%.

CONCLUSIONThe result demonstrates that magnesphere is a fast and efficient method to develop microsatellite.

DNA, Plant ; genetics ; isolation & purification ; Genetic Techniques ; Genome, Plant ; Magnetics ; Microsatellite Repeats ; Pinellia ; genetics

OBJECTIVETo study the genetic diversity of Changium smyrnioides and give a reference for utilization of the germplasm.

METHODTen different populations of Ch. smyrnioides were analyzed by the approach of sequence-related amplified polymorphism (SRAP). Genetic similarity coefficient was calculated, and systematic relationships were constructed based on the UPGMA method.

RESULTSeventeen primer pairs were selected from 160. A total of 363 bands were scored, 314 bands of them were polymorphic and the average was 18.47 polymorphic bands per primer pair, which were up to 86.50% polymorphic ratio. The results indicated that there was abundant genetic diversity among the tested materials. Genetic similarity coefficient was ranged from 0.4959 to 0.8182. Cluster analysis showed that ten different populations of Ch. smyrnioides could be distinguished into two groups.

CONCLUSIONHigh level genetic diversity was in different populations of Ch. smyrnioides, and genetic relationship was correlative to geographic position.

Apiaceae ; classification ; genetics ; Genetic Variation ; genetics ; Nucleic Acid Amplification Techniques ; methods ; Phylogeny ; Plants, Medicinal ; classification ; genetics ; Polymerase Chain Reaction