OBJECTIVETo compare shear bond strength (SBS) between composite resin core (CRC) disposed with three different surface treatments and glass ionomer cement (GIC), so that to provide theoretical basis for luting of crowns to CRC.

METHODSAccording to three different surface treatments, thirty blocks of CRC were randomly and equally divided into three groups: Roughening with diamond grit bur(RDB), RDB plus etching with Gluma Etch 35 Gel (RDBE), RDB plus coating with Adper Single Bond2 adhensive (RDBA). All CRC were cemented with GIC. All specimens were preserved in 37 degrees C water for 24 h, then SBS tests for eight specimens in each group were performed using a universal testing machine at a constant crosshead speed of 0.5 mm x min(-1). The surface topography of one CRC of each group was observed using field emission scanning electron microscope (FE-SEM) at 100 amplification. The interface between CRC and GIC was observed using FE-SEM at 500 amplification. Then the nature of failure was also recorded using FE-SEM at 25 amplification and the data were analyzed with a Kruskal-Wallis H test.

RESULTSThe surface topography of each group and the interface between CRC and GIC observed using FE-SEM were significantly different, but significant differences on the nature of failure between groups were not found (P > 0.10). SBS of each group was (4.28 +/- 0.18) MPa for RDB, (4.65 +/- 0.17) MPa for RDBE, (2.39 +/- 0.21) MPa for RDBA, respectively (P < 0.01).

CONCLUSIONThe SBS between CRC and GIC is affected by the surface treatments of CRC.

Composite Resins ; Crowns ; Dental Bonding ; Glass Ionomer Cements ; Materials Testing ; Resin Cements ; Surface Properties

In view of the problems of more artificial interventions and segmentation defects in existing two-dimensional segmentation methods and abnormal liver segmentation errors in three-dimensional segmentation methods, this paper presents a semi-automatic liver organ segmentation method based on the image sequence context. The method takes advantage of the existing similarity between the image sequence contexts of the prior knowledge of liver organs, and combines region growing and level set method to carry out semi-automatic segmentation of livers, along with the aid of a small amount of manual intervention to deal with liver mutation situations. The experiment results showed that the liver segmentation algorithm presented in this paper had a high precision, and a good segmentation effect on livers which have greater variability, and can meet clinical application demands quite well.
Algorithms ; Humans ; Image Interpretation, Computer-Assisted ; Imaging, Three-Dimensional ; Liver ; anatomy & histology

OBJECTIVETo understand the possibility of estimating the anti-inflammation effect of Liuwei Dihuang Wan (LWW) in rat by having developed HPLC-UV metabonomic technology.

METHODThe hydrophilic or lipophilic constituent group of LWW was extracted by distilled water or acetic ether respectively. The anti-inflammation effects of different LWW dosages and extractions were measured by traditional method respectively. Then, metabonomic analysis was performed.

RESULTThe high dosage of LWW extraction (16.5 g x kg(-1))could inhibit the swell of rat palm significantly, but there are not statistically significant effect of low dosage group. Nevertheless, the metabonomic study showed that LWW extraction could restore obviously the rat HPLC-UV urinary profiling disturbed by inflammation in low dosage, especially the hydrophilic constituent group.

CONCLUSIONOur study indicated that the developed metabonomic technology based on HPLC-UV might be used as a potentially powerful tool for estimating the anti-inflammation effect of LWW with sensitivity and integrity.

Animals ; Anti-Inflammatory Agents ; analysis ; metabolism ; pharmacology ; Chromatography, High Pressure Liquid ; methods ; Drugs, Chinese Herbal ; analysis ; metabolism ; pharmacology ; Male ; Metabolomics ; methods ; Rats ; Rats, Sprague-Dawley

OBJECTIVETo explore the relationship among the expression of GSK3β, PI3K/Akt signaling transduction pathway,and cytokines IL6 in Chronic Rhinosinusitis.

METHODSWe assayed mRNA and protein for GSK3β, PI3K, Akt by using reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry (IHC), and measured the cytokines IL6 mRNA by using reverse transcription polymerase chain reaction (RT-PCR) in the nasal tissue from the patients with Chronic Rhinosinusitis with Nasal Polyps (CRSwNP), Chronic Rhinosinusitis without Nasal Polyps (CRSsNP) and control subjects.

RESULTSThe relative expression levels of GSK3β, PI3K, Akt and IL-6 in CRSwNP were 0.6254 ± 0.0584, 0.8239 ± 0.7186, 0.9369 ± 0.0823 and 0.8973 ± 0.0680. But the relative expression levels of GSK3β, PI3K, Akt and IL-6 in control subjects were 0.2684 ± 0.0726, 0.3578 ± 0.0994, 0.6721 ± 0.0590, 0.5898 ± 0.0891. There were significant differences between the groups of CRSwNP and control subjects (t values were 2.358, 3.071, 2.764, and 2.239, respectively, all P < 0.05). There was no significant difference of GSK3β, PI3K, Akt and GSK3β between the groups of CRSwNP and CRSsNP (t values were 1.597, 1.842, 1.468 and 0.926, respectively, all P < 0.05). GSK3β, PI3K, Akt were not only expressed in the cytoplasm of the epithelium and gland cells, but also in the cytoplasm of the inflammatory cell. GSK3β, PI3K, Akt protein in CRS were detected at a higher rate than the normal nasal tissue (values were 16.42, 16.25 and 15.57,respectively, all P < 0.01). However there was no significant difference of GSK3β, PI3K, Akt protein between the groups of CRSwNP and CRSsNP (values were 3.27, 2.85 and 2.46, respectively, all P > 0.05). There was a positive correlation trend among the expression of GSK3β, PI3K and Akt, and IL-6 in CRS (r values were 0.645, 0.617 and 0.583,respectively, all P < 0.01).

CONCLUSIONSThe abnormal expression of IL-6, PI3K, Akt and GSK3β in the nasal mucosa of CRS may play a pro-inflammatory role in the occurrence and development of CRS.

Adolescent ; Adult ; Case-Control Studies ; Chronic Disease ; Female ; Glycogen Synthase Kinase 3 ; metabolism ; Glycogen Synthase Kinase 3 beta ; Humans ; Interleukin-6 ; metabolism ; Male ; Middle Aged ; Nasal Mucosa ; metabolism ; Phosphatidylinositol 3-Kinases ; metabolism ; Proto-Oncogene Proteins c-akt ; metabolism ; Signal Transduction ; Sinusitis ; metabolism ; Young Adult

Humans ; Nervous System Diseases ; diagnosis ; etiology ; therapy ; Purpura, Schoenlein-Henoch ; complications

Epilepsy ; etiology ; Humans ; Infant ; Male ; Nephrotic Syndrome ; complications ; congenital

Objective: To investigate the effects of astragalus polysaccharide (AP) on cardiac dysfunction in rabbits with severe scald injury. Methods: Sixty-four New Zealand white rabbits were divided into pure scald group and AP group according to the random number table, with 32 rabbits in each group. Rabbits in the two groups were all inflicted with 30% total body surface area full-thickness scald on the back. Immediately after injury, rabbits in two groups were intraperitoneally injected with lactated Ringer′s solution once for antishock. Rabbits in AP group were intraperitoneally injected with 10 mL AP solution with the dosage of 200 mg/kg 10 min after injury and the following 6 days respectively, once a day. Rabbits in pure scald group were injected with 10 mL normal saline instead. Eight rabbits of each group were respectively selected before injury hour (BIH) 1 and on post injury day(PID) 1, 3, 7, and 14 to collect blood samples from ear marginal vein, and then sacrificed immediately to collect hearts at each time point post injury. The morphology of myocardium was observed after HE staining. The serum content of cardiac troponin I (cTnI) was detected by enzyme-linked immunosorbent assay (ELISA). The serum content of aspartate transaminase (AST), creatine kinase (CK), CK isoenzyme-MB (CK-MB), lactate dehydrogenase (LDH) was detected by fully automatic chemistry analyzer. The content of angiotensin Ⅱ (Ang Ⅱ) in serum and myocardium was detected with radioimmunoassay and the content of endothelin 1 (ET-1) in serum and myocardium was detected by ELISA. Another 8 normal rabbits were sacrificed to detect the content of Ang Ⅱ and ET-1 in the myocardium as the value of the two groups of scalded rabbits at BIH 1. The serum content of superoxide dismutase (SOD) and malondialdehyde (MDA) was detected by ELISA. The values of whole blood viscosity (ηb), reductive viscosity of whole blood (ηr), plasma viscosity (ηp), hematocrit (HCT), erythrocyte rigidity index (TK), erythrocyte aggregation index (EAI), and erythrocyte sedimentation rate (ESR) were detected by fully automatic hematology analyzer. Data were processed with analysis of variance of factorial design, independent sample t test, and Dunnett test. Results: (1) Compared with those in pure scald group, the degrees of cardiomyocyte swelling, steatosis, necrosis and rupture of muscle fiber were significantly alleviated in rabbits of AP group on PID 1 and 3. There was no obvious increase in cell size, no breakage of muscle fiber or infiltration of inflammatory cells in myocardial interstitium on PID 7. The myocardial tissue structure and muscle fiber arrangement returned to normal condition on PID 14, with no interstitial fibroblast hyperplasia or excessive extra cellular matrix deposition. (2) Serum content of cTnI, CK, and LDH of rabbits in AP group was significantly lower than that in pure scald group on PID 1, 3, and 7 (with t values from 2.69 to 13.99, P<0.05 or P<0.01), while there was no significant difference between the two groups on PID 14 (with t values from -0.32 to 0.68, P values above 0.05). Serum content of AST and CK-MB of rabbits in AP group was significantly lower than that in pure scald group on PID 1 and 3 (with t values from 2.21 to 12.65, P<0.05 or P<0.01), while there was no significant difference between the two groups on PID 7 and 14 (with t values from 0.03 to 1.67, P values above 0.05). (3) Serum content of Ang Ⅱ of rabbits in AP group was significantly lower than that in pure scald group from PID 1 to 14 (with t values from 3.38 to 32.58, P values below 0.01). Serum content of ET-1 of rabbits in AP group was significantly lower than that in pure scald group from PID 3 to 14 (with t values from 3.54 to 11.02, P values below 0.01), while there was no obvious difference on PID 1 (t=0.39, P>0.05). Content of Ang Ⅱ and ET-1 in myocardial tissue of rabbits in AP group was significantly lower than that in pure scald group from PID 1 to 7 (with t values from 1.27 to 13.79, P values below 0.01), while there was no obvious difference on PID 14 (with t values respectively 0.07 and 0.81, P values above 0.05). (4) Serum content of SOD of rabbits in AP group was respectively (15.65±2.64), (14.67±0.74), and (8.43±0.56) ng/mL on PID 1, 3, and 7, which was significantly higher than (6.35±0.83), (2.62±0.75), and (2.84±0.41) ng/mL in pure scald group (with t values from -29.79 to -9.10, P values below 0.01); while there was no obvious difference on PID 14 [with (4.02±0.26) ng/mL in pure scald group and (4.11±0.52) ng/mL in AP group, t=-0.01, P>0.05]. Serum content of MDA of rabbits in AP group was respectively (1.31±0.61), (1.72±0.64), and (0.65±0.42) μmol /mL on PID 1, 3, and 7, which was significantly lower than (1.68±0.57), (2.34±0.79), and (1.06±0.32) μmol/mL in pure scald group (with t values from 1.63 to 3.16, P<0.05 or P<0.01), while there was no obvious difference on PID 14 [with (0.31±0.09) μmol/mL in pure scald group and (0.24±0.08) μmol/mL in AP group, t=2.11, P>0.05]. (5) Values of ηb1 and EAI of rabbits in AP group were significantly lower than those in pure scald group from PID 1 to 7 (with t values from 2.718 to 11.170, P<0.05 or P<0.01), while there were no obvious differences on PID 14 (with t values respectively 2.078 and -1.423, P values above 0.05). Values of ηb2 and ηr2 of rabbits in AP group were significantly lower than those in pure scald group on PID 3 and 7 (with t values from 2.178 to 19.205, P<0.05 or P<0.01), while there were no obvious differences on PID 1 and 14 (with t values from -0.730 to 1.320, P values above 0.05 ). Values of ηr1 and ESR of rabbits in AP group were significantly lower than those in pure scald group on PID 3, 7, and 14 (with t values from 3.021 to 8.058, P values below 0.01), while there were no obvious differences on PID 1 (with t values respectively 1.200 and 1.263, P values above 0.05 ). Value of ηp of rabbits in AP group was significantly lower than that in pure scald group on PID 1 (t=2.430, P<0.05), while there were no obvious differences on PID 3, 7, and 14 (with t values from 0.002 to 1.446, P values above 0.05 ). Value of HCT of rabbits in AP group was close to that in pure scald group on PID 1 (t=1.079, P>0.05), and the values were significantly lower than those in pure scald group on PID 3 and 14 (with t values respectively 3.849 and 4.208, P values below 0.01), while the value was significantly higher than that in pure scald group on PID 7 (t=-4.925, P<0.01). Value of TK of rabbits in AP group was lower than that in pure scald group on PID 7 (t=2.847, P<0.05), while there were no obvious differences on PID 1, 3, and 14 (with t values from -1.102 to 0.875, P values above 0.05). Conclusions AP can alleviate the damage of myocardium of rabbits with severe scald by reducing the production of vasoactive substances Ang Ⅱ and ET-1, decreasing oxidative stress injury by increasing the content of SOD and decreasing the production of MDA, improving blood flow performance and microcirculation perfusion.

OBJECTIVETo determine the distribution of allergens in patients with allergic rhinitis in Nanchang area and provide the clinical reference for management strategies with regional character.

METHODSPatients with symptoms of allergic rhinitis were given skin prick test (SPT) from March 2012 to May 2013. Among the patients, the distribution of all allergens was analyzed. The positive rate was compared with gender, age, occupation, season, course of disease and so on.SPSS 17.0 software was used to analyze the data.

RESULTSAmong 5 654 patients, the total positive rate of SPT was 56.07%, with dermatophagoides pteronyssinus (46.94%), dermatophagoides farina (35.99%), blomia tropicalis (31.34%) as the major allergens. With age increasing, the positive rate of allergen decreased. There was statistically significant difference (χ² = 261.372, P < 0.01) . There was significant difference of the positive rate in different occupations (χ² = 170.859, P < 0.01), the highest rate was found in student (66.12%) and the lowest was found in farmer (41.56%). There was significant difference of variation with months (χ² = 227.333, P < 0.01), the most in October, 68.88%. There was significant difference of the positive rate of dust mites in different seasons (χ² = 204.343, P < 0.01), the highest in autumn, the lowest in winter. There was no correlation between the positive rate and course of disease (P > 0.05).

CONCLUSIONSDust mites were identified as the most common allergens among patients with allergic rhinitis in Nanchang area.Occupation is one of the influencing factors for the positive rate.

Adolescent ; Adult ; Aged ; Aged, 80 and over ; Allergens ; analysis ; immunology ; Animals ; Child ; Child, Preschool ; Dermatophagoides pteronyssinus ; immunology ; Female ; Humans ; Male ; Middle Aged ; Rhinitis, Allergic ; immunology ; Skin Tests ; Young Adult

Humans ; MicroRNAs/genetics* ; Cell Line, Tumor ; Osteosarcoma/pathology* ; Neoplasm Invasiveness ; Cell Proliferation ; Bone Neoplasms/pathology* ; Cell Movement ; Neoplasm Metastasis