Environmental Monitoring ; methods ; Environmental Pollutants ; analysis ; Microchip Analytical Procedures

The treatments of infected bone defects are still a major clinical problem.In recent years,local delivery systems applied as anti-infection and bone repair material become a hot research topic.In this review,we discuss various influence factors such as osteoinductive growth factors,antibiotics,cartier material,extended release methods.We also introduce the recent progress in the development of local delivery system for the treatment of infected bone defects.

Consumer Product Safety ; Drinking Water ; standards ; Equipment Safety ; Food Safety ; Public Health ; instrumentation ; methods ; Safety Management ; Water Supply

The enzymological characterizations of site-mutagenized rat recombinant DNA polymerase?, RQ182 and RQ183 were studied The phosphocellulose column chromatographies showed that the mutant and the wild DNA polymerases were all eluted by about 0.5 mol/ L KCI, but the denatured DNA-cellulose chromatographies showed that although the wild enzyme was eluted by 0.35 mol/L KCI, RQ182 and RQ183 were eluted by 0.55 and 0.45 mol/L KCI, respectively, indicating that the binding abilities to DNA of the mutant enzymes were increased. Km values for the substrate (dTTP)of the wild enzyme, RQ182 and RQ183 were determined as 38.5, 34.5 and 111.1 ?mol/L, respectively,and the Km values for the primer (oligo(dT)) were 1.28, 1.96 and 6.58 ?g/ml, respectively. The results showed that the affinities of RQ183 to the substrate and the primer were decreased dramatically. It is suggested that Arg182 and Arg183 were involved in the active site function of DNA polymerase ?, in binding to DNA template, in recognizing of primer, and in binding to and catalyzing of substrates of the enzyme.

Objective: To optimize the technological process for the preparation of curcumin-(oleic acid)2[Cur(OA)2] loaded by mPEG5000-PLGA with modified solvent evaporation method and to characterize the nanoparticles[Cur(OA)2-NPs]. Methods: Using entrapment efficiency (EE), appearance, and particle size as indexes, orthogonal test design was used to optimize the preparation technology. Laser particle size analyzer and transmission electron microscopy (TEM) were carried out to evaluate the Cur(OA)2-NPs size, Zeta potential, and morphological characteristics. Results: The optimal preparation conditions for Cur(OA)2-NPs were as follows: Cur(OA)2-mPEG5000-PLGA (1:4); organic solvent-aqueous solvent (1:2); the stirring speed was 1200 r/min; Poloxamer 188 (F68) concentration was 0.3%. The Cur(OA)2-NPs were spherical and uniformly distributed, with average particle size 86.23 nm (range of particle size: 80-100 nm) and Zeta potential -23.8 mV. Conclusion: The modified solvent evaporation method is suitable to prepare the curcumin derivatives loaded mPEG5000-PLGA NPs.

The proposal to promote the commercialization of new insect-resistant GM cotton,GM corn and other major products in 13th Five Year Plan on National Science and Technology Innovation ,has put forward a test to the government's ability to manage GM crops cultivation.Only a few species of GM crops,not including food crops,have been commercialized in China,we lack experience in GM crops cultivation management.Taking USA,where the farmers cultivate huge areas of GM crops,as a comparison object,the examination and approval procedures before cultivation,GM crop growers' obligation of the management,the methods to reduce mingling of GM crops and non-GM crops are compared.And concludes that the differences in legal status of GM crops,the intellectual property regime and labeling regulation,the ability of relative stakeholders to implement segregation measures and bear loss lead to different regulation on GM crops cultivation management in USA and China.The government combine the domestic current situation of the agricultural environment in our country,and focus on substantial risk in the safety management of GM crops in cultivation management is advised.The competent department of agriculture,researchers and seed traders should work together to help guide growers to avoid the economic disputes due to mingling and reduce the impact on the ecological environment.

Objective To analyze the trend of transitional care in recent ten years and to find the advantages and shortages in this field between home and abroad, so as to provide reference for nursing researchers in China. Methods The literatures about transitional care pub-lished from January, 2005 to August, 2016 from Web of Science and CNKI were retrieved, and the visualizing map was drawn by operating CiteSpaceⅡ. Results The annual number publication of transitional care steadily increased in the world in recent ten years. China issued a small amount of documents before 2012, and then the research of transitional care obviously increased, while the number was comparable to the international in 2015. USA played a leading role in the field;China issued only 2%of the total, ranking the 9th. By comparing domestic and foreign research hot topics, international transitional care studies paid more attention to nursing program and the effect, while China fo-cused on the research of the disease. Research frontier showed that international transitional care service expanded to more and more sub-jects gradually, and inclined to the continuation of management, community and family support nursing research, which was relatively lack in China. Conclusion In recent years, the research of transitional care received increasing attention in China, while the depth and breadth of study was still lacking. China should learn from foreign research hot topics and frontiers to explore transitional care system, and provied pa-tients discharged nursing support and self care knowledge, to enrich high quality nursing connotation.

Objective To investigate the factors of influence in post-traumatic stress bleeding.Methods The clinical data of 1986 trauma patients and postoperative patients in recent 5 years were studied retrospectively,and cases with or without stress ulcer bleeding were comparatively analyzed.Results The incidence of stress ulcer bleeding in cases with serious injury was significantly higher than in those with mild injury(P

Along with the fast development of medical technology,the theory of holistic medical care is infiltrated into every aspect of clinical medical practices.In this paper,though the treatment of surgical hepatobiliary diseases under the direction of holistic medical care ,we emphasize the significance of holistic medical care in diagnosis and treatment ,recommend and canonize the usage of holistic medical care into the clinical practices.

Mechanism studies in the gene regulation in the eukaryotic cells is one of the momentous areas in molecular biology.Regulation at transcription level is a complex progress with multiple steps with the presence of the gene functions.There exist ubiquitous occurrence of transcription factors in mammalian tissues and biodiversity in the factors.These transcription factors are found to relate closely to various carcinogeneses,including cell proliferation,apoptosis,invasion and angiogenesis.Understanding of transcription factors and their action mechanisms,through the activation and inhibition of transcription factors,would potentially lead to the discovery of new entities in the targeting treatment and prevention of the cancer diseases.