Along with several decades development,the urinary and male genital system neoplasm has gained distinguished research achievements in China.The research output is being historically high.However,our ability of treatment and diagnosis cannot meet people's requirement of health due to the serious prevalent situation.It is necessary for China to take actions to keep up with the advanced world level.

[Obiective] To summarize the research development of the relationship between the occurrence and development of common liver diseases and miRNAs by reading and analysing the literature published in recent 10 years. [Method] By literature retrieval, we consult the literature about miRNA and the clinical research of relevant liver diseases since 2003 and summarize the regulatory mechanism of miRNAs in liver diseases. [Result] miRNAs regulate the expression of target genes at the post-transcriptional level, and they are involved in many important biological processes. In recent years, we can find that miRNAs take part in the regulation of many liver disease-related genes, and the expression level in hepatocellular carcinoma, viral hepatitis, liver fibro-sis, cirrhosis, alcoholic and non-alcoholic fatty liver change in different levels. Part of the regulatory mechanism of miRNAs has been clearly identified, suggesting that miRNAs wil be used as new therapeutic targets for liver diseases. [Conclusion] The regulatory mechanisms of miRNAs in liver diseases make a big difference for the prevention and treatment of liver diseases. These findings provide some novel thoughts about the detection, study and treat-ment about relevant liver diseases. However, many questions are stil vague and unknown, such as the net of its expression and regulation, its biological functions, and the relationship with liver diseases, and al of them are needed to get further attention.

Objective Microheterogeneity is an important problem in molecularly biological and proteomic research on malignancies.The goal of this study was to investigate the method to purify targeted cells in lung cancer and its paired normal tissues for proteomic study on lung cancer applying laser capture microdissection(LCM).Methods LCM technique was emploged to obtain the cells of lung cancer tissues and their paired normal tissues from frozen sections.Results LCM can be applied to quickly and precisely obtain pure targeted cells subgroup or single cell without interstitium under the microscope.Conclusion LCM can tackle the problem of tissue heterogeneity in molecular analysis successfully.These results indicate that LCM has a potential as a tool in lung cancer proteomic research.

Receptor of activated C-kinase 1 (RACK1) shows different expression in various tumors,because its seven WD repeating sequences can combine with a variety of molecules.In addition to activating protein kinases C,RACK1 still plays a role in many tumor-related signaling pathways.With the deepening of research,RACK1 is expected to play an active role in cancer diagnosis,treatment and prognosis.

Randomized control study of gemfibrozil and pantethine was made in 39 NIDDM patients to compare their effect on anti-lipid-peroxidation.The patients were divided into two groups at random. One group took gcmfibrozil 900 mg/d for 4 weeks, the ether group took pantethine 540 mg/d for 4 weeks. The results showed that there were no significant change in faslirg plasma glucose and HbA1 in both groups before and after treatment. The effects on lipicls and lipcproteins in gemfi-barozil and pantethine groups were as follows: TC decreased by 18.15% and 8.75% (P

Objective To evaluate the initial clinical application value of placing the Freka R Trelumina guided DSA. Methods The Freka(R) Trelumina was placed guided by DSA in 10 patients with disease of digestive tract post operation complicated with stomal leak or/and stomal stegnosis or various kinds of patients with gastric emptying disorder. Results The Freka(R) Trelumina could be put 20 cm away from Treize anadesma guided by DSA. The achievement ratio was 90%. The putting time was 1 to 3 weeks. The localization of the Freka(R) Trelumina was well. The process of feeding was successfully. The effectiveness of decompression of stomach intestine was also good. Stomal leak healed up quickly. The appearance of gastric emptying disorder disappeared. Conclusion Placing the Freka(R) Trelumina guided by DSA is a simple, safe and reliable method for jejunum nourishment and gastrointestinal decompression.

Alkaloids ; Antineoplastic Agents, Phytogenic ; Chemical Phenomena ; Chemistry ; Harringtonines

Objective To study the association of MYH9 gene single nucleotide polymorphism (SNP) with clinical manifestation,pathology and prognosis of IgA nephropathy (IgAN) patients of Han nationality population in Inner Mongolia Autonomous Region.Method One hundred and forty-eight IgAN patients proven by biopsy were enrolled in the study.Fifty-six patients were followed up for 1-97 months.DNA was extracted from the peripheral blood of above patients.PCR restriction fragment length polymorphism (RFLP) assay was used to detect the single nucleotide polymorphisms of MYH9 gene Rs3752462,Rs4821480 sites.Association of different genotypes with clinical features,pathology and prognosis im patients with IgA nephropathy was examined.Result (1) Rs3752462 site was consistent with Hardy-Weinberg equilibrium,while Rs4821480 site did not meet the Hardy-Weinberg equilibrium.(2) IgAN patients with MYH9 gene Rs3752462 site TF genotype had lower systolic blood pressure as compared to those with CC +CT genotype (P＜0.05).There were significant differences in systolic blood pressure,diastolic blood pressure and age between patients with Rs4821480 site GG genotype and patients with TT or GT genotype (P＜0.05).There were no significant differences in Scr,Ccr,plasma albumin,hemoglobin,microscopic hematuria,proteinuria,pathological HASS classification,pathological lesion among Rs4821480 site GG,TT,GT genotypes.(3) Kaplan-Meier survival analysis revealed the time from renal biopsy to renal function decline was shorted in patients with Rs3752462 site CC genotype and Rs4821480 site TT genotype.Conclusions C allele of MYH9 gene Rs3752462 site is an independent risk factor of high blood pressure damage in IgAN patients.Polymorphism of 3 genotypes of MYH9 gene Rs4821480 site is associated to the prognosis of patients.Carrying Rs3752462 site C allele and Rs4821480 site T allele may affect the prognosis of patients.

Objective To explore the related risk factors and control measures for senium patients with type 2 diabetes and to provide basis for making control and prevention measures.Methods A retrospective case-control study was conducted.548 cases of type 2 diabetes patients in our hospital were selected as case group and other 640 cases of normal people who accepted physical examination in our hospital were selected as control group.Results Exposure rates of smoking,drinking,obesity,hypertension,hypedipidemia and family history in case group were obviously higher than those of control group(P ＜0.05).Conclusion Smoking,drinking,obesity,hypertension,hyperlipidemia and family history were risk factors of type 2 diabetes.Comprehensive intervention measures related to the risk factors,such as maintaining a good way life,were importantto prevent and control type 2 diabetes.

Objective:To determine phenolphthalein illegally added into diet health products by Raman spectroscopy. Methods:Raman spectroscopy was used to determine phenolphthalein added into diet health products. The diet health products were extracted by suitable solvents, and then the extracting solution was measured by Raman spectroscopy. Results: A calibration curve was built and the analysis was performed on the samples with phenolphthalein at different concentrations. The results were accordance with the real added amount. Conclusion:With simple sample preprocessing method, Raman spectroscopy can be used in the fast detection of phe-nolphthalein illegally added into diet health products. The method is fast and simple with low cost, and can provide both qualitative and quantitative results. The detection limit is 1% for phenolphthalein in diet health products.