The prevalence of asthma in pregnant women appears to be increasing.Pregnant women with uncontrolled asthma may have an increased risk of adverse perinatal outcomes.Optimal management of asthma during pregnancy includes avoiding or controlling asthma triggers,individualized pharmacologic therapy,objective monitoring of lung function,and patient education.Inhaled corticosteroids are the preferred treatment for the management of all levels of persistent asthma during pregnancy.It is safer for pregnant women with asthma to be treated with asthma medications than to have asthma symptoms and exacerbations.The goal of asthma management during pregnancy is to optimize maternal and fetal health.

ObjectiveTo compare of the thickness of left ventricular wall of Ang Ⅱ induced cardiac hypertrophy model in both TR3 konock-out C57BL/6 mice (TR3-/- mice) and wild type C57BL/6 mice (TR3+/+ mice) by high frequency ultrasound,combined with experimental research to discuss if TR3 take part in the process of cardiac hypertrophy.Methods26 TR3 -/- mice and 33 TR3 +/+ mice were randomized to 2 groups respectively:TR3+/+ + AngⅡ group (27 mice),TR3+/+ + PBS group(6 mice),TR3-/- + Ang Ⅱ group(20 mice),TR3-/- + PBS group(6 mice).Micro-pumps with AngⅡ or PBS were placed into the subcutaneous tissue of mice to construct hypertension model in 4 weeks.Interventricular septum enddiastolic thickness (IVSd) and left ventricular posterior wall end-diastolic thickness (LVPWd) were measured by echocardiography 2 days before operation and every week after operation until 4 weeks.Then,mice were killed and mice cardiomyocytes were isolated and detected in lab (HE dyeing test,Western blotting).Results①Echocardiography:before operation,IVSd and LVPWd was not statistically different between TR3+/+ group and TR3-/- group( P ＞0.05).1 - 2 weeks after operation,IVSd and LVPWd had peaked in both TR3-/- + Ang Ⅱ group and TR3 +/+ + Ang Ⅱ group,IVSd and LVPWd in TR3 +/+ + Ang Ⅱgroup were significantly thicker than control group (TR3 +/+ + PBS group) ( P ＜0.05),IVSd and LVPWd in TR3-/-+ AngⅡ group were slightly thicker than control group (TR3-/- + PBS group) ( P ＜0.05).Compared with TR3-/- + Ang Ⅱ group,IVSd and LVPWd in TR3+/+ + Ang Ⅱ group were markedly increased( P ＜0.05).After 4 weeks operation,IVSd and LVPWd of all 4 groups were not statistically different than that in the 2nd week.②HE dyeing test:The cell size of cardiomyocytes in TR3+/+ mice increased significantly after Ang Ⅱ treatment,while TR3-/- mice did not.③ Western blotting:Ang Ⅱ promoted TR3 expressions in TR3 +/+ mice( P ＜0.05).ConclusionsTR3 do take part in the process of Ang Ⅱ induced cardiac hypertrophy in mice and it is a key factor in this process.

Objective To investigate the correlation between occurrence time and condition severity in patients of bronchial asthma complicated with allergic rhinitis. Methods The 583 outpatients with asthma in an asthma clinic during 1996-2002 were divided into simple asthma group (Group Ⅰ with 292 cases) and asthma complicated with allergic rhinitis group (Group Ⅱ with 291 cases), and their data were compared and statistically analyzed. Results The proportion of those complicated with other diseases, e.g. , nasal polyp, sinusitis, atopic dermatitis, and with a history of allergy, e.g. , food allergy, drug allergy and positive skin allergic test, was significantly higher in the Group Ⅱ than that in the Group Ⅰ ( P

Objective:To investigate the clinical value on the determination of the peripheral blood ICAM 1 level in Graves's disease(GD).Methods:The serum soluble Intercellular Adhesion Molecule 1(sICAM 1)concentration were analyzed by ELISA and the expression of ICAM 1(CD54 +) on PB lymphocytes in 37 GD patients and 22 normal subject were measured,while TsAb,TpoAb,TGAb;CD4 +,CD3 +,CD8 +,CD19 +,CD25 +were examined by FACS.Each marker between the two groups and the positive rate between the TsAb and sICAM 1 whe compared.In addition ,the relationship between the ICAM 1 level on PB and immunity markers in GD patients were analysed.Results:The mean sICAM 1 concentration was significantly higher in untreatd GD patients than in normal controls(P

? AIM: To compare the effects on the retina inner segment and retinal pigment epithelium ( RPE ) of intravitreally injecting bevacizumab, ranibizumab and aflibercept into monkey eyes.? METHODS: Fourteen healthy cynomolgus monkeys (Macaca fascicularis, aged 3-8y,10 males,4 females) were raised at the Covance Laboratories under standard conditions. The 14 monkeys were grouped into 4 groups. Three of the groups with 4 monkeys each were injected intravitreally with one of the drugs, either bevacizumab, ranibizumab or aflibercept, while the 4th group with 2 monkeys served as a negative control. On 1d and 7d of injection, 2 monkeys from each drug treatment group were sacrificed under general anaesthesia and the 4 eyes were enucleated. All the enucleated eyes were fixed in formalin, embedded in paraffin wax, cut into 4. 0 μm sections and deparaffinized according to standard procedures. Image-Pro Plus was used for all the photos to measure the content of vascular endothelial growth factor ( VEGF) in the inner segment and RPE. The ANOVA test from JMP10. 0 statistical program was used to evaluate the results.?RESULTS:Retinal sections were checked for their anti-VEGF immune reactivity. The untreated control samples had the highest level of VEGF in the RPE and inner segment. All of these three drugs can reduce the level of VEGF in the RPE and inner segment, but Avastin seems to be more effective than Eylea in this regard. Lucentis treatment at 1d seems to be more effective than Eylea at VEGF 1d. But at 7d, both Lucentis and Eylea have the same effect on reducing VEGF expression level in the RPE and inner segment.?CONCLUSION: All of these three drugs can reduce the level of VEGF in the RPE and inner segment.

Objective To investigate the clinical manifestation and outcome of severe acute respiratory syndrome (SARS).Methods The clinical data of 233 patients with SARS admitted to China-Japan Friendship Hospital from April 2003 to June 2003 were analyzed,including clinical manifestations,laboratory findings,chest radiograph,outcome and mortality of SARS.Results There were 115 male and 118 female patients in this cohort,aged 13-86 (mean 42 8?18 5yr);43 3%of the patients had one or two kinds of underlying diseases;73 0% of the patients had a history of SARS close contact. Incubation period was 1-21 days (mean 4 9?4 1d).The initial symptoms were fever in 86 3% of the patients.The respiratory symptoms were found after the onset of 1-2 weeks.The laboratory abnormalities were decreased in WBC (56 7%) and lymphocytes (78 5%).Corticosteriod was used in 69 5% of the patients.There were 10 deaths (4 3%).The elder age,underlying diseases,continued high fever, thrombocytopenia,leucocytosis and bilateral lung involvement were the risk factors.Conclusion SARS is a new and strongly contagious disease,which mainly affects youth and people in their prime life.It has its own characteristic clinical manifestations.There is high mortality in severe cases.The prognosis is poor in patients complicated with diabetes,leucocytosis,lymphocytopenia,thrombocytopenia and elevated serum LDH and CPK.

Objective To investigate the clinical and laborat ory characteristics of SARS patients complicated with underlying diseases.Methods 1 291 patients clinically diagnosed as SARS defined by Chinese Ministry of Health reported in Beijing area were analyzed retrospect ively in our study.Results In 1 291 cases,there were 27.5% patients with unde rlying diseases,in which,65.6% cases were 40 years or older.There were 72.5% p atients without underlying diseases,in which,74.6% cases were 40 years or young er.Hypertension,diabetes mellitus and coronary heart disease were the most commo n underlying diseases of SARS.Clinical symptoms and signs of SARS in underlying diseases group were more than that in without underlying diseases group (P

Objectives To study application efficiencies of two types of intestinal ostomy in the treatment of elderly patients with obstructive sigmoid colon cancer and colorectal cancer.Methods 62 elderly patients with obstructive sigmoid colon cancer and rectal cancer from January 2013 to January 2016 in our hospital were chosen.The patients were divided into loop ileostomy group(n=32) and colostomy group(n=30)depending on the type of therapy.And the stage Ⅰ and Ⅱ operative time,hospital stay,postoperative fasting time and postoperative complication incidence rates were compared between the two groups.Results The postoperative fasting time was significantly shorter in loop ileostomy group than in colostomy group during stage Ⅰ surgery(2.5 ± 0.5)d vs.(4.6 ± 0.6)d(t=14.3644,P =0.0000).In stage Ⅱ surgery,the operative time,hospital stay,postoperative fasting time (2.9 ± 0.6)d vs.(4.9 ± 0.4)d,(1.4 ± 0.4)d vs.(2.5 ±± 0.3)d,(6.3± 1.4d)vs.(8.8±1.2)d were significantly lower in the loop ileostomy group than in the colostomy group(t=-15.2762、-10.9714、-7.6601,all P =0.0000).Conclusions Compared with colostomy technique,the loop ileostomy has advantages including shorter operation time,lower incidence of postoperative complications and quicker recovery after surgery for elderly patients with obstructive sigmoid colon cancer and colorectal cancer.

Objective To evaluate the effect of injecting botulinum toxin A (BTX-A) to the external urethral sphincter on detrusor wall thickness (DWT) and the bladder function of spinal cord injury patients with detrusor-sphincter dyssynergia (DSD).Methods Twenty-one adult patients with DSD due to spinal cord injury were recruited.A single 100 IU dose of botulinum toxin A was injected into their external urethral sphincters via an uhrasound-guided transperineal route.DWT,detrusor leak-point pressure (DLPP),post-void residual volume (PRV) and maximum bladder capacity(MBC) were assessed by urodynamic examination and ultrasound evaluation before and 4 and 8 weeks after the injection.Results Four weeks later a significant decrease in PRV was observed,but no significant difference in DWT,DLPP or MBC.After eight weeks the mean DWT value had decreased by 17％,a significant reduction.There were also significant improvements in DLPP,PRV and MBC at eight weeks compared with the of baseline values.Conclusions BTX-A injection to the external urethral sphincter can reduce DWT and improve bladder function in spinal cord injury patients with DSD.

OBJECTIVE To investigate the toxicological effect of patulin(PAT)on the growth of human normal liver cells L-02 and its possible mechanisms. METHODS After cells were treated with PAT 1.25, 2.5,5,10 and 20μmol·L-1 for 24 or 48 h,cell viability was examined using MTT assay. L-02 cells were treated with PAT 5 and 10 μmol · L- 1 for 24 h ,respectively. Cytomorphology and mitochondrial membrane potential (MMP) were observed under a fluorescence microscope. Apoptosis,MMP and reactive oxygen species (ROS)were analyzed by flow cytometry. Mitochondria apoptosis pathways were detected by Western blotting. RESULTS PAT exhibited a strong inhibitory effect on L-02 in a concentration-dependent and time-dependent manner. IC50 of PAT treatment for 24 or 48 h was 6.61 and 2.78 μmol · L-1,respectively. MMP was decreased,while the percentage of low MMP cells increased from(9.2±2.3)%in controls to(23.4±4.5)%( PAT 5μmol·L-1)and(47.1±5.5)%(PAT 10μmol·L-1), respectively. Compared to untreated cells,the early apoptosis population increased from(3.8±1.1)%to(29.8±4.5)%( PAT 5μmol·L-1)and (24.1±6.2)%(PAT 10μmol·L-1)(P<0.01),respectively. Further?more,the accumulation of ROS was also observed. The effect of PAT on ROS and cell viabilities could be attenuated by glutathione. CONCLUSION PAT can significantly inhibit the growth of L-02 and induce apoptosis via ROS-dependent mitochondria pathways.