1.Differences in serum ferritin and vitamin D levels of Korean women with obesity and severe obesity
Nan Hui KANG ; Ji Sook PARK ; Hongchan LEE ; Jung-Eun YIM
Journal of Nutrition and Health 2020;53(4):381-389
Purpose:
This study examined the relationships among serum ferritin, vitamin D, folate, iron, and vitamin B12 as indicators of obesity. The results provide the basic data for the prevention and treatment of obese and severely obese people.
Methods:
This study selected 44 people from 18 years of age or older to 59 years. This study used the indicators of the body mass index (BMI) to analyze obesity as the obesity group (BMI of 25.0–29.9 kg/m2 ) and as the severe obesity group (BMI ≥ 30.0 kg/m2 ). Of the 44 subjects, 23 and 21 subjects were in the obesity and severe obesity groups, respectively. Their height, weight, body fat, skeletal muscle mass measured using bioimpedance analysis, and measured serum nutrients and biochemical parameters.
Results:
The obesity group showed a significantly lower age, body weight, BMI and body composition, body fat mass, and body fat percentage, and the height was significantly lower in the severe obesity group. The results of the biochemical parameters of the subjects showed that the levels of aspartate transaminase, alanine transaminase, hemoglobin A1c, total cholesterol, and triglyceride were within the normal range, and there was no significant difference between the 2 groups. The levels of folate, vitamin B12 , 25-hydroxyvitamin D3 , iron, and ferritin were almost normal, and there was no significant difference between the 2 groups.
Conclusion
This study revealed an association with the serum nutrients and obesity, but there was no difference between the obesity group and severe obesity group. Observations of the nutrient levels in not only the blood in obesity and severe obesity but also in red blood cells and tissues will be necessary.
2.Effect of Banxia Qinlian Decoction on Th17/IL-17 Immune Inflammatory Way of Sjögren's Syndrome NOD Model Mice.
Yan LU ; Yi CHEN ; Ya-nan WANG ; Hui LIU ; Ji-sheng ZHANG ; Wei-guo MA ; Zhi-ming SHEN ; Jie WANG ; Kang WANG ; Feng-xian MENG
Chinese Journal of Integrated Traditional and Western Medicine 2015;35(5):612-617
OBJECTIVETo explore the molecular mechanism of exocrine immune inflammatory injury of Sjögren's Syndrome and the intervention of Banxia Qinlian Decoction (BQD).
METHODSTotally 18 female NOD mice were randomly divided into the model group, the positive drug group, and the BQD group, 6 in each group. Six female BALB/c mice were recruited as a blank control group. Mice in the blank control group and the model group were gavaged with deionized water at the daily dose of 0.1 mL/10 g body weight. Tripterygium Tablet was administered by gastrogavage to mice in the positive group at the daily dose of 10 mg/kg. BQD was administered by gastrogavage to mice in the BQD group at the daily dose of 60 g crude drugs/kg. After 12 weeks of medication, mice were sacrificed. Their eyeballs were excised and blood collected. Tissues of bilateral parotids and submandibular glands were kept. mRNA transcriptional levels of IL-17, IL-6, type 3 muscarinic acetylcholine receptors (M3R), aquaporin protein-5 (AQP5) were detected by RT-PCR. Expression levels of M3R and AQP5 protein were detected by Western blot. Protein expression levels of IL-17 and IL-6 were detected by ELISA.
RESULTSCompared with the normal group, mRNA transcriptional levels and protein expression levels of IL-17, IL-6, M3R, and AQP5 were significantly up-regulated in the model group (P < 0.01). Compared with the model group, mRNA transcriptional levels and protein expression levels of IL-17, IL-6, M3R, and AQP5 were significantly down-regulated in the positive drug group and the BQD group with statistical difference (P < 0.01, P < 0.05). Compared with the BQD group, mRNA-transcriptional levels of IL-17, IL-6, and M3R, as well as M3R and AQP5 protein expression levels were significantly down-regulated in the positive drug group (all P < 0.01).
CONCLUSIONThe molecular mechanism of BQD in inhibiting SS exocrine neurotoxic injury might be possibly related to regulating Th17/IL-17 immune inflammatory way.
Animals ; Aquaporin 5 ; metabolism ; Disease Models, Animal ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Female ; Interleukin-17 ; metabolism ; Interleukin-6 ; metabolism ; Mice ; Mice, Inbred BALB C ; Mice, Inbred NOD ; Sjogren's Syndrome ; drug therapy ; immunology ; Submandibular Gland ; Th17 Cells ; Up-Regulation
3.Quantitative Detection of ID4 Gene Aberrant Methylation in the Differentiation of Myelodysplastic Syndrome from Aplastic Anemia.
Mian-Yang LI ; Yuan-Yuan XU ; Hui-Yuan KANG ; Xin-Rong WANG ; Li GAO ; Jian CEN ; Wei WANG ; Nan WANG ; Yong-Hui LI ; Li-Li WANG ; Li YU
Chinese Medical Journal 2015;128(15):2019-2025
BACKGROUNDThe diagnosis of myelodysplastic syndrome (MDS), especially hypoplastic MDS, and MDS with low blast counts or normal karyotype may be problematic. This study characterized ID4 gene methylation in patients with MDS and aplastic anemia (AA).
METHODSThe methylation status of ID4 was analyzed by bisulfite sequencing polymerase chain reaction (PCR) and quantitative real-time methylation-specific PCR (MethyLight PCR) in 100 patients with MDS and 31 patients with AA.
RESULTSThe MDS group had a higher ID4 gene methylation positivity rate (22.22%) and higher methylation levels (0.21 [0-3.79]) than the AA group (P < 0.05). Furthermore, there were significant differences between the hypoplastic MDS and AA groups, the MDS with low blast count and the AA groups, and the MDS with normal karyotype and the AA groups. The combination of genetic and epigenetic markers was used in much more patients with MDS (62.5% [35/56]) than the use of genetic markers only (51.79% [29/56]).
CONCLUSIONSThese results showed that the detection of ID4 methylation positivity rates and levels could be a useful biomarker for MDS diagnosis.
Adolescent ; Adult ; Aged ; Aged, 80 and over ; Anemia, Aplastic ; genetics ; Child ; CpG Islands ; genetics ; DNA Methylation ; genetics ; Female ; Humans ; Inhibitor of Differentiation Proteins ; genetics ; Male ; Middle Aged ; Myelodysplastic Syndromes ; genetics ; Young Adult
4.Correlation between cytochrome 3A4+894C>T P450 gene polymorphism and outcomes of coronary intervention in patients with acute coronary syndrome.
Hui LI ; Miao-Nan LI ; Pin-Fang KANG ; Yang LI ; Yang TANG ; Dong-Yu LU ; Xiao-Jun SHI ; Hong-Ju WANG
Journal of Southern Medical University 2016;37(2):261-265
OBJECTIVETo investigate the relationship between plasma cytochrome P450 3A4 (CYP3A4) 894C>T gene polymorphism and the risk of recurrence of adverse cardiac events after percutaneous coronary intervention (PCI) in patients with acute coronary syndrome (ACS).
METHODSA total of 275 patients with ACS received standard dual antiplatelet therapy and PCI. Platelet aggregation rate (PAR) was detected in each patient before and 7 days after administration of the anti-platelet drugs. Single nucleotide polymorphism of CYP3A4 gene 894C>T was detected with PCR and microarray technique. The number of coronary artery lesions was determined by PCI and the Gensini score was calculated. The patients were followed up for 3-12 months after discharge.
RESULTSNo significant difference was found in CYP3A4 gene polymorphism between patients with clopidogrel resistance (CR group) and those without CR (NCR group) (P>0.05). Multivariate logistic regression analysis showed that CYP3A4 gene 894C>T polymorphism was not correlated with CR in patients with ACS (OR 1.359, P>0.05). During the follow-up, the incidence of cardiovascular events was significantly higher in CR group than in NCR group (P<0.05), but this difference was not related to the mutation type of 894C>T locus of CYP3A4 gene.
CONCLUSIONThe CYP3A4 gene 894C>T polymorphism is not associated with the effect of anti-platelet therapy and the risk of cardiovascular event in patients with ACS following PCI.
Acute Coronary Syndrome ; therapy ; Alleles ; Blood Platelets ; Cytochrome P-450 CYP3A ; genetics ; Humans ; Percutaneous Coronary Intervention ; Platelet Aggregation ; Platelet Aggregation Inhibitors ; therapeutic use ; Platelet Function Tests ; Polymorphism, Single Nucleotide ; Ticlopidine ; analogs & derivatives ; therapeutic use
5.Protein profiles of multinodular hepatocellular carcinoma with multicentric occurrence or with intrahepatic metastasis.
Mei LI ; Kun GUO ; Xiao-nan KANG ; Lu SUN ; Hong SHU ; Ruo-lin LI ; Ming-hui XU ; Yin-kun LIU ; Xue QIN ; Shan LI
Chinese Journal of Hepatology 2009;17(5):354-358
OBJECTIVETo analyze the protein expression profiles of multinodular hepatocellular carcinoma (HCC) with multicentric occurrence (MO) or with intrahepatic metastasis (IM).
METHODS5 IM and 6 MO patients were divided into groups of IM1, IM2, MO1 and MO2 according to the size of node of HCC. Two dimensional gel electrophoresis (2-DE) and mass spectrum were used to analyze the protein expression profiles. Western blot was used to confirm the results obtained by mass spectrum.
RESULTS2-DE of IM1, IM2, MO1 and MO2 indicated that 30 protein dots were differentially expressed in these tumors. By mass spectrum, 25 proteins were identified. Gene ontology classification indicated that these proteins are associated to cell movement, signal transduction, oxidoreduction, lipid metabolism, and amino acid metabolism.
CONCLUSIONThe protein expression profiles of IM is different from that of MO, 2-DE and mass spectrum can be used to identify the molecular markers of IM and MO of HCC.
Adult ; Blotting, Western ; Carcinoma, Hepatocellular ; metabolism ; pathology ; Electrophoresis, Gel, Two-Dimensional ; Humans ; Liver Neoplasms ; metabolism ; pathology ; Male ; Middle Aged ; Neoplasm Metastasis ; Neoplasms, Multiple Primary ; metabolism ; pathology ; Prognosis ; Proteome ; metabolism ; Proteomics
6.Bone Forming Gene Therapy (Immune Animal Model in Ex Vivo Gene Therapy for Spinal Fusion with Type 5 Adenoviral Delivery of the LIM Mineralization Protein-1 cDNA).
Hak Sun KIM ; Hui Wan PARK ; Hwan Mo LEE ; Seong Hwan MOON ; Jin Oh PARK ; Jung Won HA ; Nan Hyun KIM ; Young Ho KANG ; Scott D BODEN
Journal of Korean Society of Spine Surgery 2001;8(4):437-446
STUDY DESIGN: In vivo study to determine the immune effects to adenoviral vector encoding LMP-1 cDNA in rabbit. OBJECTIVE: To quantify the immune effect of Ad5-LMP-1 in the rabbit during the therapeutic gene transfer. SUMMARY OF LITERATURE REVIEW: One of the major limitations in the use of adenoviral vector for gene therapy is the immune response and it made the poor transduction efficiency when re-administrated. Adenoviral antigen plus those derived from transgene expression in transduced cell contribute to cellular, humoral and non-specific immune response constitutes barriers to successful gene therapy. Therefore, the animal immune model will be mandatory to study the immune impact. MATERIALS AND METHOD: We i.v. injected Ad5-betaGal to total 24adult NZW rabbits; 1x108, 1x109, 1x1010, 1x1011v.p. to each 6 rabbits allowed them to develop immune response. Six non-immunized animals were used as control. Adenovirus antibodies were measured at 0, 4, 8, 16, 20 weeks. Group I. 6 control rabbit underwent spinal arthrodesis at 4 weeks (n=3) and 16 weeks (n=3) with 4 million cells and MOI of 4. Group II. 6 rabbit underwent spinal arthrodesis at 4 weeks after injection of 108 p.f.u virus (n=3) and 16 weeks (n=3). Group III. six 109 immunized rabbits, Group IV. six 1010 immunized rabbits, Group V. six 1011 immunized rabbits, underwent spinal arthrodesis at 4 and 16 weeks after injection. Total anti-Ad Ig and neutralizing antibody titer was measured on the 0. 4. 8, 16, 20 weeks after injection. RESULTS: Group I. All 6 non-immunized rabbits had solid spine fusions at 4 and 16 weeks. Group II. All 3immunized rabbits had not spine fusions at 4 weeks and all three had solid spine fusion at 16 weeks. Group III. None of them (n=6) immunized rabbits had spine fusion at 4 and 16weeks, but some bone formation was observed at 16 weeks. Group IV, V. None of them immunized rabbits had bone formation. The anti-Ad5 Ig and neutralizing Ab were detected and peaked at the 4weeks and significantly dropped off 16 weeks after injection. CONCLUSION: This experiment revealed that a small dose of adenovirus elicited an enough immune response that inhibited the bone formation. Because majority of human posses the Ab against adenovirus, it will be mandatory to overcome immune response in adenoviral vector gene therapy.
Adenoviridae
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Animals*
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Antibodies
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Antibodies, Neutralizing
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Arthrodesis
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DNA, Complementary
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Genetic Therapy*
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Humans
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Models, Animal*
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Osteogenesis
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Rabbits
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Spinal Fusion*
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Spine
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Transgenes
7.Incidence of osteonecrosis of the femoral head in divers: an epidemiologic analysis in Dalian
Dewei ZHAO ; Lei YANG ; Fengde TIAN ; Benjie WANG ; Daping CUI ; Lin GUO ; Nan WANG ; Ying WANG ; Baoyi LIU ; Ning AN ; Weimin FU ; Shibo HUANG ; Wenqiang GU ; Hui XIE ; Chaoqiang WANG ; Wenfeng LUO ; Feiri HUANG ; Kai KANG ; Pengfei LIU
Chinese Journal of Orthopaedics 2012;32(6):521-525
Objective To investigate the incidence of osteonecrosis of the femoral head (ONFH) in divers of Dalian.Methods From January 2010 to December 2010,all registered 855 divers in Dalian were enrolled in this study.All divers underwent a unified medical examination and a questionnaire including height,weight,blood pressure and hip inspection.Radiological examination (anteroposterior and frog position)was used for all divers.Suspicious persons with hip pain but normal X-ray performance were confirmed by MRI.Results Sixty-eight divers were confirmed as ONFH,and the incidence of ONFH in divers of Dalian was 7.95%.According to the Ficat classification,12 patients (12 hips) were in Phase 1,40 patients (47 hips)in phase Ⅱ,3 patients (3 hips) in phase Ⅲ,13 patients (15 hips) in phase Ⅳ.The mean age of divers was 32.6+5.5 years (range,18-55 years).The onset ages of most patients ranged from 30 years to 50 years,accounted for 83.82% (57/68).Among all patients,primary school education accounted for 10.58% (38/359),junior high school education 6.28% (28/446),high school education 4.26% (2/47),university education 0 (0/3); seniority less than 5 years accounted for 4.55% (20/440),6-10 years 9.69% (28/289),11-15 years 13.04% (12/92),16-20 years 21.05% (4/19),more than 20 years 26.67% (4/15); self-employed accounted for 11.88% (19/160),private enterprise 8.41% (38/452),and national enterprise 4.53% (11/243).Conclusion The incidence of ONFH is high in divers of Dalian,which is different in terms of age,seniority,level of education,enterprise nature.
8.The correlation between thyroid function and the severity of coronary artery lesions
Nan YU ; Ying GAO ; Yan-Jun GONG ; Fan YANG ; Zhi WANG ; Lin QIU ; Qi KANG ; Fang LIU ; Yang YU ; Xiao-Hui GUO
Chinese Journal of Interventional Cardiology 2017;25(12):671-676
Obiective Tend to explore the correlation between thyroid function and the severity of coronary artery lesions in chinese coronary heart disease(CHD)patients. Methods A total of 558 patients who underwent coronary angiography at the cardiology department of Peking University First Hospital from January 2013 to June 2015 were enrolled in this retrospective study. All patients were divided into coronary heart disease group and non-coronary heart disease group. Thyroid hormone levels were tested in all patients before angiography,and clinical characteristics,lipid profiles and SYNTAX scores were also obtained. Results Of the 558 patients,409 were diagnosed of CHD(73.3%),and among them,5 patients were hyperthyroid/subclinical hyperthyroid(1.2%),13 patients were hypothyroid/subclinical hypothyroid(3.2%),14 patients had euthyroid sicknesssyndrome(ESS)(3.2%),377 patients were euthyroid(92.2%). Among the 149 non-CHD patients(26.7%),3 patients were subclinical hyperthyroid(2.0%),8 patients were hypothyroid/subclinical hypothyroid(5.4%),2 patients were ESS(1.3%),172 patients were euthyroid(91.3%). The proportion of patients with ESS in the CHD group was significantly higher than that of the non-CHD group (3.4% vs. 1.3%,P=0.018). Except for the patients with ESS,FT3 level was significantly lower the in CHD group than that in the non-CHD group[(4.52±0.57)pmol/L vs.(4.65±0.63)pmol/L,P=0.015]. There were no significant differences in FT4,T3,T4 levels between the two groups(P>0.05). In the CHD group,there was an association between the SYNTAX score groups and free triiodothyronine(FT3)levels(F=6.260,P=0.002). A significant correlation was also observed between the FT3 level and the number of coronary artery lesions(F=5.691, P=0.004). There was no correlation between the SYNTAX score groups or number of coronary artery lesions and thyroid hormone levels,respectively. There were no correlations between lipid profiles and thyroid function. Patients were further divided into three subgroups according to their serum TSH levels. The prevalence of CHD is significantly higher in the subgroup with elevated TSH(85.7%)than in the subgroup with normal TSH(68.6%, P=0.022). Conclusions FT3 level is weakly associated with the severity of CHD. Higher TSH level may be a risk factor of CHD.
9.Analysis of pathogen spectrum and antimicrobial resistance of clinical common organisms from twelve teaching hospitals in 2016
He′nan LI ; Ji ZENG ; Yan JIN ; Zhidong HU ; Kang LIAO ; Yanping LUO ; Chao ZHUO ; Rong ZHANG ; Xiuli XU ; Wenen LIU ; Yingmei LIU ; Yunzhuo CHU ; Hui WANG
Chinese Journal of Laboratory Medicine 2018;41(9):651-657
Objective To investigate the spectrum and antimicrobial resistance of major pathogens causing nosocomial infections in China, 2016. Methods Non-duplicated nosocomial cases as well as pathogens causing bloodstream infections ( BSI) , hospital-acquired pneumonia ( HAP) and intra-abdominal infections ( IAI ) from 12 teaching hospitals across China were collected. The minimum inhibitory concentrations (MICs) of important clinical common strains were determined by agar dilution method or broth microdilution method. The CLSI M100-S27 criteria was used for interpretation. Data were analyzed by using WHONET-5. 6 software. Results A total of 2060 cases were collected, including 894 cases from BSI, 630 cases from HAP and 536 cases from IAI. The MICs of 1896 important clinical common strains were determined. Escherichia coli and Klebsiella pneumoniae were the most prevalent pathogens causing BSI and IAI, while Acinetobacter baumanii and Pseudomonas aeruginosa were dominated in HAP. All Staphylococcus aureus were susceptible to tigecycline, linezolid, daptomycin and glycopeptides. Methicillin-resistant S. aureus accounted for 44. 4% ( 75/169 ) of all the S. aureus. The rate of methicillin-resistant coagulase-negative staphylococci was 80. 9% ( 72/89 ) . No Enterococcus strains were found resistant to tigecycline, linezolid or daptomycin. Vacomycin resistant enterococcus was found in Enterococcus faecium, accounting for 1. 8% ( 2/111 ) of all E. faecium strains. Tigecycline, meropenem, amikacin, imipenem, and polymyxin B exhibited high potency against Enterobacteriaceae and the susceptibility rates were 96. 6%(865/895), 94. 3% (859/911), 94. 2% (858/911), 94. 1% (857/911), and 91. 6% (820/895), respectively. The prevalence of extended-spectrum β-lactamase was 58. 4% ( 263/450 ) in E. coli and 28. 6% ( 84/294 ) in K. pneumonia. The rate of carbapenem resistant K. pneumonia and E. coli was 15. 3% ( 45/294 ) and 1. 8% ( 8/450 ) , respectively. The percentage of polymyxin B resistant K. pneumonia and E. coli was 4. 1% ( 12/294 ) and 4. 4% ( 20/450 ) , respectively. The rate of tigecycline resistant K. pneumonia and E. coli was 2. 4% ( 7/294 ) and 0. 2% ( 1/450 ) , respectively. A. baumanii showed low susceptibility to the antimicrobial agents except tigecycline ( 91. 4%, 235/257 ) and polymyxin B (100%, 257/257). The rate of carbapenem resistant A. baumanii was 80. 5% (207/257). The rate of carbapenem resistant P. aeruginosa was 31. 7% ( 59/186 ) . Polymyxin B and amikacin demonstrated high antibacterial activity against P. aeruginosa with susceptility rate of 100% ( 186/186 ) and 90. 9% ( 169/186), respectively. Conclusions Nosocomial pathogens showed high susceptibilities against tigecycline and polymyxin B. Antimicrobial resistance in A. baumannii is a serious problem. The prevalence of carbapenem-resistant Enterobacteriaceae and polymyxin B resistant Enterobacteriaceae has increased, which should be monitored continuously in China.
10.Outcome of catheter ablation of atrial fibrillation in patients with prior ischemic stroke.
Song-Nan LI ; Jun-Ping KANG ; Xin DU ; Xiao-Nan HE ; De-Yong LONG ; Rong-Hui YU ; Ri-Bo TANG ; Cai-Hua SANG ; Chen-Xi JIANG ; Man NING ; Jian-Zeng DONG ; Chang-Sheng MA
Chinese Medical Journal 2013;126(6):1033-1038
BACKGROUNDCatheter ablation for atrial fibrillation (AF) has been demonstrated to be effective in a subsets of patients with AF. However, very few data are available in regard to patients with prior history of stroke undergoing catheter ablation. This study aimed to investigate the outcome of catheter ablation in AF patients with prior ischemic stroke.
METHODSBetween January 2008 and December 2011, of 1897 consecutive patients who presented at Beijing An Zhen Hospital for treatment of drug-refractory AF, 172 (9.1%) patients in the study population had a history of ischemic stroke. All patients underwent catheter ablation and were followed up to assess maintenance of sinus rhythm and recurrence of symptomatic stroke.
RESULTSAmong these 1897 patients, 1768 (93.2%) who had complete follow-up information for a minimum of six months were included in the final analysis. Patients in the stroke group (group I) and the no-stroke group (group II) were similar in regards to gender, body mass index (BMI), history of diabetes, type of AF, and left atrial size. The patients in group I were older than those in group II, and had a higher incidence of hypertension, chronic heart failure, lower left ventricular ejection fraction (LVEF), and higher CHADS2 scores. Six months after ablation, 107 (68.6%) patients in group I and 1403 (87.1%) in group II had discontinued warfarin treatment (P < 0.001). During a median follow-up of (633 ± 415) days, 65 patients in the group I and 638 in group II experienced AF recurrence, and five patients in group I and 28 in group II developed symptomatic stroke. The rates of AF recurrence and recurrent stroke were similar between group I and group II (41.7% vs. 39.6%, P = 0.611; 3.2% vs. 1.7%, P = 0.219; respectively).
CONCLUSIONCatheter ablation of AF in patients with prior stroke is feasible and efficient.
Aged ; Atrial Fibrillation ; surgery ; Catheter Ablation ; methods ; Female ; Humans ; Male ; Middle Aged ; Retrospective Studies ; Stroke ; surgery