Objective To detect the expression of islet amyloid polypeptide (IAPP) in the pancreatic tissues of patients with pancreatic cancer,and to explore the relation between diabetes and pancreatic cancer.Methods Surgically-removed 28 pancreatic cancer samples and adjacent normal pancreatic tissues were studied.Among these patients there were 12 patients complicated with diabetes while other 16 patients did not.Immunohistoehemieal method and Western blot method were used to detect the expression of IAPP in different tissue specimens.Results lAPP expressed in human pancreatic islet cell cytoplasm.Immanohistochcmical index of pancreatic cancer and adjacent normal pancreatic specimens of patients with diabetes,and pancreatic cancer and adjacent normal pancreatic specimens of patients without diabetes were 283 305±91 627,122 874±86 917,154 032±81 097 and 105 797±67 593,respectively;the relative IAPP expressions were (173.1±23.5) %,( 119.4±18.4) %,( 148.7±28.3 ) % and 100%.Irrespective of the presence of diabetes,expression of IAPP in pancreatic cancer specimens was significantly higher than that in adjacent normal pancreatic specimens ( P ＜ 0.01 );expression of IAPP in pancreatic cancer plus diabetes specimens was significantly higher than that in pancreatic cancer specimens without diabetes (P ＜0.01 );expression of IAPP in adjacent pancreatic specimens complicated with diabetes was significantly higher than that in adjacent pancreatic specimens without diabetes ( P ＜ 0.05).Conclusions LAPP participated in the course of diabetes and pancreatic cancer,and may be the common pathogenesis of the two diseases.

BACKGROUND: Homocysteine is associated with the attack of cerebral infarction, and cystathionine-beta-synthase (CBS) is a key enzyme of the metabolism of homocysteine, but it is still not clear whether its gene mutation is the potential candidate genic factor of cerebral infarction.OBJECTIVE: To observe the correlation of CBS base mutation at T833C and G919 sites with the attack of ischemic stroke in youths from the angle of genic mutation.DESIGN: A patient-control analysis.SETTING: Department of Neurology, China-Japan Friendship Hospital of Jilin University.PARTICIPANTS: Patient group (n=100): Young inpatients with cerebral infarction ≤ 45 years old in the China-Japan Friendship Hospital of Jilin University between April 2003 and December 2004, admitted within 2 days after attack. Control group (n=100): Normal young physical examinees in this hospital at the same period.METHODS: The levels of fasting and loaded homocysteine in plasma were detected with high performance liquid chromatography (HPLC), CBS genes at T833C and G919A sites with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and amplification refractory mutation system in all the subjects.RESULTS: All of the 200 subjects entered the analysis of results. ① Detection of CBS genes at T833C and G919A sites: The distribution of genotype, frequency of homozygote and that of allele had no significant differences between the patient group and control group (P ＞ 0.05). ② Concentration of homocysteine in plasma: There were significant differences among the genotypes at G919A and T833C sites (P ＜ 0.001). The results of LSD-t test of the mutation at the two sites showed that there were significant differences between homozygote and heterozygote, homozygote and wild type,as well as C heterozygote and wild type (P ＜ 0.05).CONCLUSION: ① The CBS gene mutations at both T833C and G919A sites can lead to the obvious increase of the concentration of homocysteine in plasma. ② The CBS gene mutations at T833C and G919A sites had no direct association with the attack of cerebrovascular disease in youths.

BACKGROUND: The increase of concentration of plasma homocysteine is the independent risk factor of atherosclerotic and thrombotic cerebral infarction. Genic mutation of methylene tetrahydrofolate reductase (MTHFR), which is the metabolic enzyme of homocysteine in thansulfuration and remethylation,can induce the elevation of the concentration of plasma homocysteine.OBJECTIVE: To explore the relationship of hyperhomocysteinemia and genic mutation of MTHFR of homocysteine with ischemic cerebrovascular disease in youths.DESIGN: Case-control observation,SETTING: Department of Neurology, China-Japan Friendship Hospital,Jilin UniversityPARTICIPANTS: 100 young patients with cerebral infarction, who were hospitalized within 2 days after episode at the Department of Neurology,China-Japan Union Hospital, Jilin University between April 2003 and December 2004, were enrolled as case group, 73 males and 27 females, aged 27-45 years old with an average of (42±5) years. 100 cases in control group were healthy people receiving health examination in the same period, 70 males and 30 females, aged 18-45 years old with an average of (39±4) years.METHODS: The homocysteine in fasting plasma of testees was detected with high performance liquid chromatograpy (HPLC). C677T site and A1298C site of MTHFR gene were analyzed with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and detected with armplification refractory mutation system (ARMS).MAIN OUTCOME MEASURES: Detection of MTHFR C677T and A1298C gene; Relationship between concentration of plasma homocysteine and MTHFR genotype.RESULTS: A total of 100 inclusive patients and 100 normal control people were involved in the result analysis. ①Detection of MTHFR C677Tand A1298C gene: Distribution of genotype, frequency of homozygote and frequency of allele of MTHFR C677T in the case group and control group had significant difference (P ＜ 0.01 ) while the distribution of genotype,frequency of homozygote and frequency of allele of MTHFR A1298C gene in the case group and control group had insignificant difference (P ＞ 0.05 ). ②Relationship between the concentration of plasma homocysteine and MTHFR genotype: The concentration of plasma homocysteine between MTHFR C677T and A1298C genotype had significant difference (P＜ 0.001 ). The mutant result LSD-t check of the 2 sites showed that mean difference of homozygote and heterozygote, homozygote and concentration of wild type homocysteine had statistical significance (P ＜ 0.05). The mean difference of MTHFR C677T and A1298C heterozygote and concentration of wild type homocysteine in plasma had no statistical significance (P＞ 0.05 ).CONCLUSION: The mutation of MTHFR C677T and A1298C leads to the marked increase in the concentration of plasma homocysteine. The MTHFR C677T polymorphism site is the independent risk factor of is chemic cerebrovascular disease in youths. The genic mutation of MTHFR A 1298C has no correlation with the attack of ischemic cerebrovascular disease of youths.

ObjectiveTo study the effects of angiogenesis on the development of endometrial carcinoma. Methods Hysterectomy specimens were stained immunohistochemically by the marker of factor Ⅷ-related antigen for endothelium vessels in normal controls, patients with endometrial hyperplasia and with endometrial carcinoma. Results The microvascular density(MVD) in tumour increased gradually from normal endometrium to endometrial hyperplasia and to endometrial carcinoma(P<0.01). MVD correlated with the mvometrial invasion, histologic grades and the stages.ConclusionMYD in endometrial hyperplasia increases. MVD can be used as a prognostic factor.

Objective To analyze the volatile composition in the leaves of Sabina chinensis L. Ant. and Sabina chinensis L. Ant. cv. Kaizuca and to study their effects on bacteria. Methods GC-MS was employed in the analysis of volatile composition and four kinds of bacteria were used for testing the sterilization and bacteriostasis of the volatile oil. Results The main substance in volatile oil from the two kinds of plants, Sabina chinensis L. Ant. and Sabina chinensis L. Ant. cv. Kaizuca, was bornyl acetate and the percentage was 38.1% and 46.5% respectively. In addition, in the volatile oil from Sabina chinensis L. Ant. contained 24% of phellandrne and 12.4% of p-menth-l-en-4-o1, as for Sabina chinensis L. Ant. cv. Kaizuca, 30.0% of limonene and 7.9% of ?-pinene were contained. The volatile oil from Sabina chinensis L. Ant. had greater effects of bacteriostasis and sterilization on Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli compared with Sabina chinensis L. Ant. cv. Kaizuca. Conclusion Compared with Sabina chinensis L. Ant. cv. Kaizuca, the effects of sterilization and bacteriostasis of volatile oil from Sabina chinensis L. Ant. are much greater and with a larger spectrum of bacteria.

Objective To study the toxicity and physical, chemi ca l and antimicrobial properties of the tracheal catheter made with nanometer anti microbial plastics. Methods For examining the toxicity of the n ew tracheal catheter, 30 Kunming mice were divided into 3 groups. The mice in gr oup A received 2ml of fluid sucked through the catheter by intra-abdominal inje ction, the mice in group B received 1ml of suction fluid, and the mice in group C received 2 ml of normal saline. Multiple proportional dilutions were used to d etect the antimicrobial property of the tracheal catheter against both positive and negative Gram bacteria. Results No noxious effects or toxic ity was found in tested mice, and all the physical and chemical data of the cath eter were up to the National Standard. The catheter showed a certain antimicrobi al effects on some of common pathogenic bacteria. Conclusion Th e tracheal catheter made with nanometer antimicrobial plastics had no noxious ef fects on mice, it showed a certain antimicrobial effects on to some of common pa thogenic bacteria, and its physical and chemical properties were up to the Natio nal Standard.

The rutile structure titanium oxide (Ti-O) film was prepared on the pure titanium material TA2 (99.999%) surface by the magnetic filter high vacuum arc deposition sputtering source. The method can not only maintain the material mechanical properties, but also improve the surface properties for better biocompatibility to accommodate the physiological environment. The preparation process of the Ti-O film was as follows. Firstly, argon ions sputtered to the TA2 substrate surface to remove the excess impurities. Secondly, a metal ion source generated Ti ions and oxygen ions by the RF discharge. Meanwhile a certain negative bias was imposed on the sample. There a certain composition of Ti-O film was obtained under a certain pressure of oxygen in the vacuum chamber. Finally, X-ray diffraction was used to research the structure and composition of the film. The results showed that the Ti-O film of the rutile crystal structure was formed under the 0.18 Pa oxygen partial pressure. A Nano scratch experiment was used to test the coating adhesion property, which demonstrated that the film was stable and durable. The contact angle experiment and the platelet clotting experiment proved that the modified surface method had improved platelet adhesion performance, and, therefore, the material possessed better biocompatibility. On the whole, the evaluations proved the modified material had excellent performance.
Heart Valves ; Heart, Artificial ; Ions ; Materials Testing ; Oxygen ; Surface Properties ; Titanium ; X-Ray Diffraction

AIM: To study the pharmacokinetics of phenolic acids from Mailuoning injection in rats. METHODS: SD rats were given a single i.v. administration dose of Mailuoning injection 10 mL/kg, plasma and urine were collected before and after injection. Phenolic acid components in plasma and urine were measured by LC/MS. Pharmacokinetic parameters were determined from the plasma concentration-time data and urinary excretion-time data with the DAS software package. RESULTS: After i.v. of Mailuoning injection, chlorogenic acid (CGA), 1, 5-dicaffeylquinic acid (1,5-DCQA), 3, 4-dicaffeylquinic acid (3,4-DCQA), 3, 5-dicaffeylquinic acid (3,5-DCQA) and caffeic acid (CA) were quickly excrectioned. The t1/2 of CGA, 1,5-DCQA, 3,4-DCQA, 3,5-DCQA and CA were 0.649, 0.334, 0.479, 0.486 and 0.330 h, respectively. AUC0-∞ were (22.522±2.716), (0.353±0.062), (3.620±1.246), (5.287±1.627) and (2.257±0.360) mg·L-1·h, respectively. After i.v. of Mailuoning injection, CGA, 1,5-DCQA, 3,4-DCQA, 3,5-DCQA and CA can all be detected in the urine. The amounts of CGA, 1,5-DCQA, 3,4-DCQA, 3,5-DCQA and CA excreted from urine during 0-24 h were (122.22±26.49)%, (3.30±1.26)%, (0.24±0.11)%, (1.93±0.77)% and (18.61±4.99)% of dose given in rats, respectively. CONCLUSION: After i.v. of Mailuoning injection, phenolic acids can be excreted quickly. Only a small quantity of 1,5-DCQA, 3,4-DCQA, 3,5-DCQA and CA were excreted from urine. 3,4-DCQA and 3,5-DCQA may be metabolized into CGA in the rat plasma.

ObjectiveTo established a cell line that expresses hBD1 stably,and detected the antimicrobial activity of the hBD1 to the muhidrug resistant bacterial strains.MethodsRecombinant plasmid was introduced into COS-7 cells by lipofectamine,cells were selected in culture medium containing G418 to acquired the monoclonal cell lines,total RNA were extracted from the cultured cells,expression levels of hBD1 mRNA was identified by RT-PCR,collected the supernatant solution of the cultured cell,expression levels of protein was identified by Western blot.Put the expression products and resistant organisms mixed together,after incubation in different times in 37℃,coating the mixtures in LB flat,then obtained the ratios between colonies number of experimental groups and colonies number of control groups,put those ratios as the survival rate of the drug resistance bacterias.Results The monoclonal cell lines had obtained after screened with G418,the hBD1 gene could be detected both at transcriptional and protein levels,Under the influence of expression product hBD1,survival rate of muhidrug-resistant Acinetobacter baumannii,multidrug-resistant Escherichia coli and multidrug-resistant Klebsiella pneumoniae could reduced to 9%,22% and 50%,but survival rate of multidrug-resistant Stenotrophomonas maltophilia is not have apparente difference with the control group.ConclusionThe stably-transfected cell line of hBD1 was successfully constructed,and the expression products of hBD1 showed the antimicrobial activity toward multidrug resistant bacterial strains.

Objective To explore the quantitative expression of estrogen receptor(ER),progesterone receptor(PR)in breast hyperplasia and its Clinical significance.Methods The expression of ER and PR was examined by immunohistochemical S-P method and quantitative analysis technique in 20 cases a piece of epithelial hyperplasia of usual type,atypical hyperplasia(mild,moderate,severe)and intraductal carcinoma,which contrasted with 20 cases of usual breast.Results The difference of the expression of ER and PR between usual breast or epithelial hyperplasia of usual type and atypical hyperplasia or intraductal carcinoma was significant(P50%,there was significant difference between moderate dysplasia and mild dysplasia,but no significant difference between moderate dysplasia and severe dysplasia,intraductal carcinoma.Conclusions The abnormal expression of ER and PR may be an early event in the progression of breast carcinoma,it could be used for early diagnosis of breast premalignancy and as a effective mark to monitor the prognosis.