OBJECTIVE:To adopt gel method for the determination of bacterial endotoxin in Fat emulsion(10%)/amino acid (15)/glucose (20%) injection. METHODS:According to the gel method in term ofbacterial endotoxin test methodin Chinese Pharmacopeia(2015 edition),the maximal valid dilution(MVD)of samples were determined through interference test and the vali-dated. The results were compared with chromogenic method. RESULTS:In gel method,the interference to agglutination reaction of TAL and bacterial endotoxin can be excluded when samples were diluted 24 times or less. In chromogenic method,the samples should be diluted 76 times or less. CONCLUSIONS:Gel method can be used for bacterial endotoxin test of Fat emulsion(10%)/amino acid(15)/glucose(20%)injection.

Objective To investigate the correlations of serum eukaryotic translation promoter 2α(eIF2α)and activated transcription factor 4(ATF4)levels with the degree of renal tissue injury and renal function in patients with diabetic nephropathy(DN).Methods A total of 102 patients with DN(DN group)and 102 patients with simple diabetes(control group)were selected.According to the severe degree of renal tissue damage,the patients with DN were divided into microalbuminuria group(MG group,35 cases)and dominant albuminuria group(PG group,41 cases)and renal dysfunction group(RIG group,26 cases).Serum levels of eIF2α,ATF4,urea nitrogen(BUN),creatinine(Scr),cystatin C(CysC)and estimated glomerular filtration rate(eGFR)were measured.Pearson correlation analysis was used to explore the correlations of eIF2α and ATF4 with BUN,Scr,CysC and eGFR;multivariate Logistic regression analysis was used to explore the risk factors of DN;receiver op-erating characteristic(ROC)curve was used to analyze the value of eIF2α and ATF4 in diagnostic of DN.Results Serum levels of eIF2α,ATF4,BUN,Scr and CysC in the DN group were higher than those in control group,eGFR was lower than that in control group(P＜0.05).Serum eIF2α and ATF4levels in the RIG group were higher than those in PG and MG groups(P＜0.05).Serum eIF2α and ATF4 levels in DN patients were positively correlated with BUN,Scr and CysC,and neg-atively correlated with eGFR(P＜0.05).Long duration of diabetes,higher body mass index,high level of eIF2α and high level of ATF4 were risk factors for DN(P＜0.05).The area under the curve of eIF2α and ATF4 in diagnosis DN was 0.770 and 0.799,respectively,and the area under the curve of their combined diagnosis was 0.879,which was higher than that of single diagnosis(P＜0.05).Conclusion Serum levels of eIF2α and ATF4 are increased in patients with DN,which is related to the severity of renal injury and renal dysfunction in DN.The combination of eIF2α and ATF4 is of high value in the diagnosis of DN.

Objective To investigate the correlations of serum eukaryotic translation promoter 2α(eIF2α)and activated transcription factor 4(ATF4)levels with the degree of renal tissue injury and renal function in patients with diabetic nephropathy(DN).Methods A total of 102 patients with DN(DN group)and 102 patients with simple diabetes(control group)were selected.According to the severe degree of renal tissue damage,the patients with DN were divided into microalbuminuria group(MG group,35 cases)and dominant albuminuria group(PG group,41 cases)and renal dysfunction group(RIG group,26 cases).Serum levels of eIF2α,ATF4,urea nitrogen(BUN),creatinine(Scr),cystatin C(CysC)and estimated glomerular filtration rate(eGFR)were measured.Pearson correlation analysis was used to explore the correlations of eIF2α and ATF4 with BUN,Scr,CysC and eGFR;multivariate Logistic regression analysis was used to explore the risk factors of DN;receiver op-erating characteristic(ROC)curve was used to analyze the value of eIF2α and ATF4 in diagnostic of DN.Results Serum levels of eIF2α,ATF4,BUN,Scr and CysC in the DN group were higher than those in control group,eGFR was lower than that in control group(P＜0.05).Serum eIF2α and ATF4levels in the RIG group were higher than those in PG and MG groups(P＜0.05).Serum eIF2α and ATF4 levels in DN patients were positively correlated with BUN,Scr and CysC,and neg-atively correlated with eGFR(P＜0.05).Long duration of diabetes,higher body mass index,high level of eIF2α and high level of ATF4 were risk factors for DN(P＜0.05).The area under the curve of eIF2α and ATF4 in diagnosis DN was 0.770 and 0.799,respectively,and the area under the curve of their combined diagnosis was 0.879,which was higher than that of single diagnosis(P＜0.05).Conclusion Serum levels of eIF2α and ATF4 are increased in patients with DN,which is related to the severity of renal injury and renal dysfunction in DN.The combination of eIF2α and ATF4 is of high value in the diagnosis of DN.

ObjectiveFocused on the laboratory animal domestication and breeding of domestic cats, to explore the feeding management methods and breeding techniques of experimental cats. MethodsSeven Chinese garden cats from three litters were introduced from the rural suburbs of Guangzhou, and a breeding seed colony was established. The cats were domesticated in captivity, bred, closed breeding and transmission according to the feeding and management methods of laboratory animal. The population reproduction, the number of pregnancies per year, the litter season, the birth and weaning quality of the cats, and the survival rate of weaning were statistically collected. ResultsThe young breeding cats were able to adapt to the cage feeding management. In the transmission breeding and the expanded breeding colony, the number of female cats pregnant with one, two or three litters a year accounted for 63.2%, 26.3% and 10.5%, respectively. The proportions of litters born from the 1st to the 4th quarters were 20.7%, 20.7%, 27.6%, and 31.0%. A total of 29 pregnancies and 101 kittens were got from 19 female cats, with an average of (3.5±1.33) kittens per litter. The birth weights of female and male cats were (89.31±13.69) g and (93.47±15.12) g, respectively. Sixty-seven kittens survived from weaning. The average survival rate was 60.86%, and the weaning weights of female and male cats were (361.62±82.77) g and (376.0±91.71) g, respectively. ConclusionDomestic Chinese garden cats can adapt to laboratory animal feeding and breeding rules, and have strong fertility. They can normally pregnant and breeding throughout the year. The kittens grow to 5-6 months of age can meet the weight requirements for the examination of pharmaceutical hypotensive substances, and can be used as experimental cats for pharmaceutical examination with clear origin.