Various concentrations, including 100%, 75%, 50%, and 25% of dimethyl sulfoxide (DMSO) were applied on the back of albino rats, followed by ultraviolet irradiation after 30 minutes. Biopsy specimens were taken 6, 12, 24, 36, and 48 hours afters ultraviolet irradiation without local anesthesia. The macroscopic and histologic findings were as follows. 1. Mild degree of erytheme appeared only on the site of the highest concentration (100%) of DMSO. 2. Vacuolization of the prickle cells appeared slightly later on the sites of higher concentrations (75% and 100%) than those of lower concentrations and control. 3. After 48 hours post-irradiation, there was no detectable vacuolization on the sites of higher concentrations, whereas marked vacuolization still remained on the other sites. 4. More pronounced epidermal thickening could be observed on the sites of higher concentrations than the sites of lower concentrations and control. 5. The dermal edema was more completely disappeared on the sites of higher concentrations than the sites of lower concentrations and control, 48 hours after ultraviolet irradiation.
Anesthesia, Local ; Animals ; Biopsy ; Dimethyl Sulfoxide* ; Edema ; Rats

Conservative or surgical methods have been used for the treatment of fracture of mandibular subcondyle, but consensus has not been reached in regard to the proper management of this injury. Several problems related to the surgical procedure have led many surgeons to avoid surgical management of condylar fractures in favor of closed reduction and intermaxillary fixations. However, luxation of the condyle may lead to various long-term complications such as open bite on the contralateral side, dysfunction, deviation in opening and closing movements, as well as bone apposition leading to ankylosis. As a consequence, open reduction is preferable when the condyle is displaced. Various methods-for example, direct wiring technique, simple bone plating, dynamic compression plating-have been advocated for the treatment of fracture of mandibular subcondyle. Of such methods, a functionally-stable osteosynthesis can be achieved by compression osteosynthesis methods. Lag screw osteosynthesis is essentially a form of compression osteosynthesis in which the bone fragments are bound to one another as a result of traction from the screw. An advantage of compression osteosynthesis is that the end of a fractured bone can be maintained in an opposed position under pressure, and then primary bone healing occurs by direct osteoblastic activity within the fracture. As well, it obviates or reduces the need for maxillomandibular fixation, as well as the morbidity associated with conventional treatment methods such as facial nerve injury and bone resorption due to wide dissection. We have treated 9 cases of displaced subcondylar fractures of the mandible with the lag screw fixation system which is marketed by the Martin corporation. Mobilization and guidance of the jaw was begun from 7 or 10 days postoperatively. The radiologic and clinical evaluations showed good repositioning of the fragments and good occlusion postoperatively The disadvantage is that surgical procedures are relatively compound, and an additional procedure - removal of lag screw - is necessary after complete bone union. Lag screw osteosynthesis can be added as a another valid armament for plastic surgeons in the management of mandibular subcondyle fractures.
Ankylosis ; Bone Resorption ; Consensus ; Facial Nerve Injuries ; Jaw ; Jaw Fixation Techniques ; Mandible ; Open Bite ; Osteoblasts ; Traction

No abstract available.
Cartilage* ; Enophthalmos* ; Ribs* ; Transplants*

The series comprised 424 cutaneous reactions to drugs treated at the department of Dermatology, Medical college of Korea University, during the years 1969-1975. The whole series comprised 211 male(49. 8%) and 213 female(50. 2%) patients. Exanthernatous eruptions are the most frequent of all cutaneous manifestation of drug reactions. Antipyretic analgesics, antibiotics and antibacterial drugs were the three group inducing most drug reactions in the present study, following by herb drugs. Of the antipyretic anaIgesics, acetylsalicylic acid was the ones most of the involved, and sulfonamides were the most frequent cause of skin reactions induced by antibacterial drugs and penicillin and tetracycline were the frequent cause of skin reactions induced by antibiotics.
Analgesics ; Anti-Bacterial Agents ; Aspirin ; Dermatology ; Drug Eruptions* ; Humans ; Korea ; Penicillins ; Skin ; Sulfonamides ; Tetracycline

No abstract available.
Cleft Lip*

Clinical and bacteriological studies, including seasitivity tests, of 85 patients in pyodermas such as impetigo contagiosa, pustular acne vulgaris, hair follicle and sweat gland infections, and secondary pyogenic infections superimposed on primary dermatoses were carried out during 4 months period, from June 1970 to Sept.1970, at department of dermatology, schooI of medicine, Seoul national university. The results were as follows; 1) Predominant age group of impetigo was the preschool. ages (87%). 2) Causative agents of impetigo in our 30 cases were coagulase positive staphylococcus aureus only in 27, streptococcus only in 0 and both organisms in 3 (one case wasmixed infection with s. aureus and b-hemolytic streptococcus, and two were mixed infections with s. aureus and a-hemolytic streptococcus). 3) Culturing from l2 cases of pustular acne vulgaris, we found coagulase negative staphylococcus albus in 9, coagulase positive stsphylococcus aureus in 1 and no growth in 2. 4) In 35 cases of hair follicle and sweat gland infections, we isolated coagulase positivestaphylococcus aureus only in 32, coagulase negative staphylococcus albus only in 2, and mixed infection with staphylococcus aureus and b-hemolytic streptococcus in l. 5) Isolated organisms in 8 cases of secondary pyogenic infections superimposed on primary dermatoses were coagulase positive staphylococcus aureus only in 2, coagulase negative staphylococcus albus only in 1, b-hemolytic streptococcus only in 1, a- hemolytic streptococcus only in 1, and mixed infections with various combination in 3. 6) Results of sensitivity tests with isolated organisms in the above diseases groups were as follows; (a) Total 66 strains of coagulase positive staphylococcus aureus showed resistance to ampicillin in 97.0%, penicillin in 78.8%, terramycin in 72.7%, rizynomycin in 71.2%, streptomycin in 24.1%, chloramphenicol in 16.7%, neomycin in 12.1%, kanamycin in 4.5% and leukomycin in 1.5%, (b) Total 8 strains of hemolytic streptococci were sensitive to chloramphenicol, leukomycin and penicillin.
Acne Vulgaris ; Ampicillin ; Chloramphenicol ; Coagulase ; Coinfection ; Dermatology ; Hair Follicle ; Humans ; Impetigo ; Kanamycin ; Neomycin ; Oxytetracycline ; Penicillins ; Pyoderma* ; Seoul ; Skin Diseases ; Staphylococcus ; Staphylococcus aureus ; Streptococcus ; Streptomycin ; Sweat Glands

No abstract available.
Cleft Lip* ; Humans ; Lip*

No abstract available.
Ointments*

Growth factors influencing the function of chondrocytes are insulin-like growth factor I(IGF-I), basic fibroblast growth factor(bFGF), transforming growth factor-beta1(TGF-beta1), and epidermal growth factor(EGF). To find out the role of four kinds of growth factors in the biosynthesis of type I and II collagen represented as the phenotype of the disc cells, we cultured the disc cells isolated from rabbit intervertebral discs primarily and then checked cell proliferation, the expression of type I and II procollagen mRNA, and the immunohistochemical stains with type I and II collagen antibodies during in vitro culture in the maintenance medium containing low serum concentration with adding four kinds of growth factors. The results are as follows. FBS(10% Fetal bovine serum) group showed the highest cell proliferation potential. EGF and TGF groups showed remarkable cell proliferation, but there was no significant difference in IGF and FGF groups comparing to control group. A partial clone that encodes the rabbit type II procollagen C-propeptide region(RbCo12A1) was successfully isolated by reverse transcription-polymerase chain reaction using total RNA extracted from articular chondrocytes of rabbits. The identity of the cDNA clone was confirmed by DNA sequencing of the polymerase chain reaction products. A comparison of human al(II) cDNA sequence showed high sequence homology(83.6%). Type I procollagen mRNA expressed highly in EGF group. FGF, IGF, and TGF groups showed no significant expression comparing to control group. FBS group showed lower expression than control group. Type II procollagen expression was increased with passage of time, so at Day 10 it was the highest in all groups. Control group showed the highest expression among 6 experimental groups. The expression of type II procollagen in FGF and TGF groups was slightly lower than that of control. EGF and IGF groups showed markedly decreased expression comparing to control group. That in FBS group was the lowest, so it was three times lower than control group. In immunohistochemical stains with type I collagen, there was no difference among control, FBS, and EGF groups. FGF, IGF, and TGF groups showed increased positivity on stain comparing to control group, but the positivity didnt exceed 10%. For type II collagen, EGF and FGF groups showed decreased positivity, but there was no significant difference in FBS, IGF, and TGF groups comparing to control group. On the basis of this study, it may be concluded that TGF-pl showed the possibility of regeneration or delay the degeneration process of the intervertebral disc through the contribution to the stimulatory effects of cell proliferation and the synthesis of type II collagen. For the clinical use of this, more studies about the combination effects with FBS or other kinds of growth factors and finding out the ideal concentration about TGF-pl will be needed.
Antibodies ; Cell Proliferation ; Cells, Cultured* ; Chondrocytes ; Clone Cells ; Collagen Type I ; Collagen Type II ; Collagen* ; Coloring Agents ; DNA, Complementary ; Epidermal Growth Factor ; Fibroblasts ; Humans ; Intercellular Signaling Peptides and Proteins* ; Intervertebral Disc* ; Phenotype ; Polymerase Chain Reaction ; Procollagen ; Rabbits ; Regeneration ; RNA ; RNA, Messenger ; Sequence Analysis, DNA

No abstract available.
Cicatrix* ; Diskectomy* ; Laminectomy* ; Rabbits*