1.Management of Risk of Statin Therapy.
Hee Jeong CHOI ; Jong Nam PARK
Journal of the Korean Academy of Family Medicine 2004;25(10):713-720
No abstract available.
Hydroxymethylglutaryl-CoA Reductase Inhibitors*
2.Characteristics of the Immortalized Human B-cells by Epstein-Barr Virus.
Ho Jong JEON ; Bong Nam CHOI ; Yoon Kyeong OH
Korean Journal of Pathology 1997;31(9):832-846
Human lymphoblastoid B-cell lines immortalized by Epstein-Barr virus (EBV) were established from peripheral blood of patients with acute myeloblastic and chronic lymphocytic leukemia and chronic fatigue syndrome. The sera of patients with acute myeloblastic and chronic lymphocytic leukemia did not show antibodies to Epstein-Barr viral capsid antigen (VCA), but serum of a patient with chronic fatigue syndrome disclosed antibodies to VCA (IgG, IgM), and EBNA was demonstrated in peripheral blood mononuclear cells by polymerase chain reaction. The established cell lines were mature B-cell phenotypes with polyclonal proliferation in early passage and no evidence for commitment to other lineages. The immortalized cells by EBV were designated as CSUP-1 and CSUP-2 (from acute myeloblastic leukemia, FAB classification M2 and M1), CSUP-3 (from chronic lymphocytic leukemia) and CSUP-4 (from a patient with chronic fatigue syndrome). The CSUP-1, 2, 3, and 4 grew in suspension forming clumps with a doubling time of 38 to 49 hours. Colony formation was not recognized in plate. By light and electron microscopic examination, the immortalized cells showed features of lymphoblastoid to plasmacytoid lymphocytes, and multinucleated giant cells. The lymphoblastoid cells showed scanty cytoplasm with poorly developed organelles. Immunophenotypic analyses of CUSP-1, 2, 3, and 4 with monoclonal antibodies by flow cytometry showed B-cell phenotype with polyclonal proliferation in early passage. Epstein-Barr virus nuclear antigen was confirmed in the extracted DNAs from immortalized cells by polymerase chain reaction. DNA analysis showed a normodiploid stemline with a DNA index of 1.12. The established cells were strongly reactive for CD10, CD30 (Ki-1) in early passage, and bcl-2 and c-myc onco-protein in early and late passage. Karyotypic analysis of CSUP-1, 2, 3 and 4 showed 46, XY or 46, XX. No tumorigenesis in heterotransplanted SCID mouse was recognized. This immortalized cells by EBV should be a valuable cell lines to study the pathogenesis of EBV-related malignant lymphoma.
Animals
;
Antibodies
;
Antibodies, Monoclonal
;
B-Lymphocytes*
;
Capsid
;
Carcinogenesis
;
Cell Line
;
Classification
;
Cytoplasm
;
DNA
;
Fatigue
;
Fatigue Syndrome, Chronic
;
Flow Cytometry
;
Giant Cells
;
Granulocyte Precursor Cells
;
Herpesvirus 4, Human*
;
Humans*
;
Leukemia, Lymphocytic, Chronic, B-Cell
;
Leukemia, Myeloid, Acute
;
Lymphocytes
;
Lymphoma
;
Mice
;
Mice, SCID
;
Organelles
;
Phenotype
;
Polymerase Chain Reaction
3.A study on voice rehabilitation after total laryngectomy.
Youn Woo NAM ; Jong Ouck CHOI ; Kwang Sun LEE
Korean Journal of Otolaryngology - Head and Neck Surgery 1991;34(2):316-323
No abstract available.
Laryngectomy*
;
Rehabilitation*
;
Voice*
4.Experimental study of interstitial Nd:YAG laser hyperthermia on rabbit tongue mucosa.
Youn Woo NAM ; Kwang Yoon JUNG ; Jong Ouck CHOI
Korean Journal of Otolaryngology - Head and Neck Surgery 1993;36(5):977-989
No abstract available.
Fever*
;
Mucous Membrane*
;
Tongue*
5.Embryonal Rhabdomyosarcoma of the Biliary Tree: A Case Report.
Ki Nam LEE ; Duck Hwan CHUNG ; Kyung Jin NAM ; Jong Young OH ; Byung Ho PARK ; Jong Chul CHOI
Journal of the Korean Radiological Society 1995;32(4):639-642
Rhabdomyosarcoma are reportedly the most common soft tissue sarcoma occuring in childhood, but the biliary tree is a rare site of origin for this tumor. Recently we experienced a case of embryonal rhabdomyosarcoma of the biliary tree in a 30-month-old child. UItrasonography showed hypoechoic mass filling the dilated left. intrahepatic & extrahepatic bile ducts, and CT showed hypodense mass with heterogeneous enhancement after contrast infusion. Intraoperative cholangiography showed filling defects within the dilated left. intrahepatic & extrahepatic bile ducts. Postoperative MRI showed residual mass within the left. intrahepatic duct which was hypointense on T1WI and hyperintense on T2WI.
Bile Ducts, Extrahepatic
;
Biliary Tract*
;
Child
;
Child, Preschool
;
Cholangiography
;
Humans
;
Magnetic Resonance Imaging
;
Rhabdomyosarcoma
;
Rhabdomyosarcoma, Embryonal*
;
Sarcoma
6.Embryonal Rhabdomyosarcoma of the Biliary Tree: A Case Report.
Ki Nam LEE ; Duck Hwan CHUNG ; Kyung Jin NAM ; Jong Young OH ; Byung Ho PARK ; Jong Chul CHOI
Journal of the Korean Radiological Society 1995;32(4):639-642
Rhabdomyosarcoma are reportedly the most common soft tissue sarcoma occuring in childhood, but the biliary tree is a rare site of origin for this tumor. Recently we experienced a case of embryonal rhabdomyosarcoma of the biliary tree in a 30-month-old child. UItrasonography showed hypoechoic mass filling the dilated left. intrahepatic & extrahepatic bile ducts, and CT showed hypodense mass with heterogeneous enhancement after contrast infusion. Intraoperative cholangiography showed filling defects within the dilated left. intrahepatic & extrahepatic bile ducts. Postoperative MRI showed residual mass within the left. intrahepatic duct which was hypointense on T1WI and hyperintense on T2WI.
Bile Ducts, Extrahepatic
;
Biliary Tract*
;
Child
;
Child, Preschool
;
Cholangiography
;
Humans
;
Magnetic Resonance Imaging
;
Rhabdomyosarcoma
;
Rhabdomyosarcoma, Embryonal*
;
Sarcoma
7.Effects of Cyclosporine on the Intrarenal Renin-Angiotensin System.
Jong Seong KIM ; Mi Ra AN ; Nam Ho KIM ; Ki Chul CHOI ; Jong Un LEE
Korean Journal of Nephrology 1998;17(5):679-685
The present study was aimed at investigating the effect of cyclosporine A (CsA) on the renal renin-angiotensin systems. In rats chronically treated with CsA, the intrarenal expression of various genes of the renin-angiotensin system was assessed by Northern blot analysis. Along with the increases in plasma and renal renin activities, chronic CsA-treatment differentially affected the renal expression of renin-angiotensin system. The treatment with CsA for one week did not significantly alter the expression of either type 1 angiotensin II receptor (AT1A) or angiotensinogen gene, but increased the renin mRNA level. The three-week-treatment caused increases in the expression not only of renin but also of AT1A and angiotensinogen genes. Supplementation with L-arginine kept the expression of renin mRNA normal in the one-week-treated, but failed to prevent the alterations of the gene expression in the three-week-treated. Feedback control among components of the renin-angiotensin system also influences angiotesinogen. In the liver, the expression of angiotensinogen mRNA was decreased by the CsA-treatment for either one- or three-weeks. In conclusion, chronic CsA-treatment is associated with a differential expression of various genes for the renin-angiotensin system. L-Arginine may be effective in maintaining the normality of renin-angiotensin system only during early period after beginning the use of CsA.
Angiotensinogen
;
Animals
;
Arginine
;
Blotting, Northern
;
Cyclosporine*
;
Gene Expression
;
Liver
;
Plasma
;
Rats
;
Receptors, Angiotensin
;
Renin
;
Renin-Angiotensin System*
;
RNA, Messenger
8.A case of thanatophoric dwarfism.
Sung Jin HWANG ; Kyung Nam CHOI ; Jong Hyun CHOI ; Doo Yong CHUNG ; Kyung Rok SEUNG
Korean Journal of Obstetrics and Gynecology 1993;36(11):3803-3808
No abstract available.
Thanatophoric Dysplasia*
9.A study on CT brain scanning of the patients without neurologicsigns in the patient group who had visited an emergency room.
Seon Hyang JANG ; Nam Hyen CHOI ; Jong Tae CHOI ; Eui Shik CHUNG
Journal of the Korean Academy of Family Medicine 1991;12(8):28-37
No abstract available.
Brain*
;
Emergencies*
;
Emergency Service, Hospital*
;
Humans
10.A study of the factors influencing the compliance of hypertensivepatients to therapy.
Nam Hyeon CHOI ; Ki Hong KIM ; Jong Tae CHOI ; Ki Soon KIM
Journal of the Korean Academy of Family Medicine 1991;12(10):1-12
No abstract available.
Compliance*