BACKGROUND: Arterial blood gas analysis are highly susceptible to preanalytic error due to improper method of obtaining or handling the blood sample before analysis. The error in measurement of blood gas analysis are loss of CO2 by exposure to atmospheric air, effect of anticoagulant itself, temperature difference between the experimental subject and the measuring electrode and metabolic change which occur between blood sampling and measurement. METHOD: To study the effect of the delay in estimation of blood gas and drawn blood on values of blood gas partial pressure and pH. Blood sample were divided into 2 groups according to the method of storage, group I stored at 24~25degrees C(room temperature) under anaerobic condition. ;group II stored at 0~4degrees C(refrigerator) under anaerobic condition. The samples were analyzed by time interval through 180 minutes in each group. RESULTS: The result were as follows: 1) PaO2 decreased significantly after 10 mins in group I, whereas it decreased significantly after 20 mins in group II. 2) PaO2 increased significantly after 20 mins in group I, whereas it increased significantly after 120 mins in group II. 3) pH decreased significantly after 60 mins in group I, whereas it decreased significantly after 120 mins in group II. 4) No significant changes of bicarbonate and SaO2 were noted in each group CONCLUSION: From above results, it would be advisable to analyze the sample in a short period of time or to store in a refrigerator when the measuring will be delayed. So we highly recommend that blood gas analysis should be performed as soon as possible after sampling, especially within 10 minutes.
Blood Gas Analysis ; Electrodes ; Hydrogen-Ion Concentration ; Partial Pressure

No abstract available.

PURPOSE: We experienced two cases of rhegmatogenous retinal detachment after LASIK procedure. This is the first published report, so we report this with literature review. METHODS: A 40-year-old female who had undergone LASIK procedure a month before complained sudden drop in vision and on fundus examination, retinal detachment with two retinal tears was detected. And 22-year-old male who had LASIK procedure 30 months before complained sudden drop in vision and on fundus examination, retinal detachment with three retinal holes was detected. RESULTS: Retinal reattachment was achieved with vitrectomy in the first case and scleral buckling using silicone sponge in the second case and the retina remained reattached during follow-up period. CONCLUSIONS: Clinicians should be aware of retinal pathology predisposing to retinal detachment in patient undergoing LASIK.
Adult ; Female ; Follow-Up Studies ; Humans ; Keratomileusis, Laser In Situ* ; Male ; Pathology ; Porifera ; Retina ; Retinal Detachment* ; Retinal Perforations ; Retinaldehyde* ; Scleral Buckling ; Silicones ; Vitrectomy ; Young Adult

Conjunctivochalasis is an isolated bilateral condition in which redundant conjunctival tissue overlies the lower eyelid margin or covers the lower pmctum. It produces tearing by mechanical obstruction of the lower punctum and/or mechanical disruption of normal outflow of the lacrimal film. A modified surgical technique, simple local excision of redundant conjuctiva in the crescent shape along the plica semilunaris and the lower bulbar conjunctiva between limbus and lower fornix, relieved epiphora in two male patients with redundant conjunctiva near the lower punctum. Surgery was done under the local anesthesia without postoperattve complications. Authers emphasize the importance of careful examination of conjunctivochalasis during ocular examination of patient complaining tearing.
Anesthesia, Local ; Conjunctiva ; Eyelids ; Humans ; Lacrimal Apparatus Diseases ; Male

We reviewed the medical records of 201 patients who were admitted between January 1980 and March 1992 with a diagnosis of traumatic hyphema to define risk factors associated with the development of rebleeding. Rebleeding occurred in 18 patients (9.0%), and took place on day 1 to 6 after injury with a maximum occurrence on day 2. A group of 32 patients who were examined more than 24 hours after injury were found to have a rebleeding rate of 18.8% (six of 32 patients). It was higher than the rebleeding rate (6.8%) in patients examined within 24 hours. When the amount of blood is more than 1/3 of anterior chamber at the time of initial examination, rebleeding was shown to occur more frequently. Children had a rebleeding rate similar to adults, and males showed approximately the same rate of rebleeding compared to females. This review suggests that patients examined more than 24 hours after injury and injured severely represent unique population that deserves special consideration.
Adult ; Anterior Chamber ; Child ; Diagnosis ; Female ; Humans ; Hyphema* ; Male ; Medical Records ; Risk Factors*

No abstract available.
Constriction, Pathologic*

Takayasu's arteritis is the chronic inflammatory panarteritis of unknown pathogenesis, affecting aortic arch and main arotic branches, pulmonary and renal arteries. It mainly affects young oriental women and cause chronic ischemic injury to tissues of the brian, orbits, upper limbs, myocardium, and kidneys. The ophthalmologic features are tend to be late the manifestations of Takayasu's arteritis and include ischemia of the retina, choroid, and anterior segment. We experienced two cases of Takayasu's arteritis whose ipsilateral ophthalmic artery was completely occluded. yet maintained proper visual acuity by external carotid-ophthalmic anastomosis.
Aorta, Thoracic ; Choroid ; Female ; Humans ; Ischemia ; Kidney ; Myocardium ; Ophthalmic Artery ; Orbit ; Renal Artery ; Retina ; Takayasu Arteritis* ; Upper Extremity ; Visual Acuity

Blow-out fracture is a fracture of the weadk bony orbital floor resulting from the transmission of intraorbital pressure in a blunt trauma. In this report, I analyse the clinical aspects of 27 patients who were treated with cyanoacrylate during open reduction. The leading cause was a traffic accident(17 patients, 63%). In addutuib. the inferior wall of the orbit was the most frequent site of fracture(22 patients, 81%). The interval between the onset of injury and the operatuib averaged 7.3days. The most common clinical sign helpful in diagnosing the blow-out fracture is the lid sign-ecchymosis and edema(26 patients, 96%); but, diplopia, positive forced duction test and enophthalmos were found in under 30% of the cases. The operations were done by infraciliary approach in most cases, and a silastic sheet was firmly attached to the site of fracture with cyanoacrylate. In all cases examined, there were no significant complications. In conclusion, I am convinced that the use of cyanoacrylate during the placement of silastic sheet for the treatment of blow-out fracture makes a firm adhesion, prevents reherniation of tissue and protrusion of the implant and also reduces complications.
Cyanoacrylates* ; Diplopia ; Enophthalmos ; Humans ; Orbit ; Orbital Fractures*

Carbonic anhydrase, an enzyme catalysing the reversible hydration of carbon dioxide, is present in the Muller cells.Because the enzyme is not present in other uroretinal cells in the retina, it can be used as a marker for Muller cells.Carbonic anhydrase activity was demonstrated bnzymehistochemically in human and rabbit Muller cells to know a relation of metabolic functions and carbonic anhydrase activity.Human retinas were obtained from donor eyes.The eyes were enucleated immediately after death forenzymatic activity. In human retina, heavy staining was found in the inner nuclear layer and nerve fiber layer, moderate staining in the outer nuclear layer and weak or no staining in the plexiform layers.In rabbit retina, heavy staining was found in the nerve fiber layer and nuclear layers and weak reaction in the two plexiform layers. These findings suggest that Muller cells may participate in CO2 homeostasis mechanism of carbonic anhydrase in the retina.
Carbon Dioxide ; Carbon* ; Carbonic Anhydrases ; Ependymoglial Cells ; Homeostasis ; Humans ; Metabolism ; Nerve Fibers ; Retina ; Tissue Donors

PURPOSE: The histiogenesis of retinoblastoma, the most common intraocular malignancy of childhood, has been investigated from the early times. But in spite of this effort, its origin has been controversial. This study was performed to investigated the cell of origin for retinoblastoma using enzyme histomchemistry for carbonic anhydrase. METHODS: We obtained enucleated eye that was diagnosed as retinoblastoma and its section was stained for hematoxylin-eosin for diagnosis of retinoblastoma. We used enzyme histomchemistry for carbonic anhydrase distinguishing Muller's cells, red-and green-sensistive cones from neuro-retinal cells. RESULTS: They were disagnosed as relatively well-differentiated retinoblastoma by hematoxylin-eosin staining and composed of tumor cells with numerous rosette. Neither numeric nor morphologic changes of Muller cells that are suspected of malignant features in enzyme histochemistry for carbonic anhydrase was found. CONCLUSIONS: The cells of retinoblastoma were originated from the two layers, inner nuclear and ganglion cell layer. The enzyme histochemistry for carbonic anhydrase is the one of the useful methods to investigate the origin of retinoblastoma although more cases is needed to assess.
Carbonic Anhydrase I ; Carbonic Anhydrases ; Diagnosis ; Ependymoglial Cells ; Ganglion Cysts ; Retinoblastoma*