Objective To detect the changes in protein profile of rats'lung inhaled radon using two- dimensional electrophoresis(2-DE)and MS.Methods Male Wistar rats were exposed to radon with the cumulative dose up to 100,200 and 400 working level months(WLM).The total proteins of rats'lung were extracted and isolated by 2-DE.The different protein expressions were analyzed with ImageMaster 2D Platinum software and obviously altered proteins were digested by trypsin and identified by MALDI-TOF-MS.Resuits Comparison of the 2-DE images between the control and radon-exposed groups indicated 14 up-regulated and 9 down-regulated protein spots,15 of which were identified by MALDI-TOF or MALDI-TOF/TOF MS.Conclusions The 2-DE image of radon groups'lung has changed,and the lung damage effect of radon is related to some proteins.

Objective To explore the value of spectral CT imaging in the preoperative evaluation on histodifferentiation of rectal adenocarcinoma.Methods 90 patients with rectal adenocarcinoma underwent dual-phase enhanced spectral CT scan,and were divided into well,moderately and poorly differentiated adenocarcinoma groups according to the pathology.Single-energy images with energy levels from 40 to 140 keV were generated by GSI Viewer software,and the slope K value of the energy curves were calculated.Iodine concentrations were derived from iodine-based material-separation images and normalized to the iodine concentrations in the aorta. ROC curves were derived to evaluate the differentiation diagnosis efficiency of normalized iodine concentration (NIC)and slope K in rectal adenocarcinoma,respectively.Results There were 22,50 and 18 cases in the well,moderately and poorly differentiated group,respectively.The iodine concentration,NIC and slope K value were statistically different both in the arterial and venous phase (P<0.05).According to the ROC curve,the diagnostic value of NIC was close to that of the slope K,with the sensitivity>76% and the specificity>74% in the arterial phase,and the sensitivity>77% and the specificity>70% in the venous phase by choo-sing the appropriate threshold.Conclusion The spectral CT can provide a new method for preoperatively evaluating the histodiffer-entiation of rectal adenocarcinoma.

Objective:To investigate the value of intravoxel incoherent motion diffusion weighted imaging (IVIM-DWI) parameters combined with T 2WI texture analysis of primary lesions of rectal adenocarcinoma in preoperative prediction of lymph node metastasis with short diameter ≤9 mm. Methods:Retrospective analysis was performed on 115 cases of rectal adenocarcinoma confirmed by surgical pathology in Affiliated Provincial Hospital of Anhui Medical University from June 2015 to October 2020. All patients underwent total mesorectal resection and received conventional rectal MRI and IVIM-DWI scan before surgery. According to the pathological results of lymph node, the patients were divided into lymph node metastatic group ( n=44) and non-metastatic group ( n=71). IVIM-DWI parameters of primary rectal adenocarcinoma were measured including apparent diffusion coefficient (ADC), diffusion coefficient (D), pseudo diffusion coefficient (D *) and perfusion fraction (f). The region of interest (ROI) of the whole lesion of rectal adenocarcinoma was delineated on axial T 2WI; then the ROIs were imported into GE Analysis Kit software to extract 3D texture feature. The differences of IVIM-DWI parameters and texture feature parameters were compared between two groups using independent sample t test or Mann-Whitney U test. The optimal texture feature parameters with independent predictive function were screened by multivariate logistic regression. Then the texture feature model and combined model based IVIM-DWI and texture feature parameters were established. The receiver operating characteristic (ROC) curves were used to evaluate the performances of IVIM-DWI, texture feature parameters, texture feature model and combined model in predicting lymph node metastasis in patients with rectal adenocarcinoma. The area under the ROC curve (AUC) were compared with DeLong test. Results:Among all the IVIM-DWI parameters, the D * and f values of primary rectal adenocarcinoma were significantly different between the lymph node metastasis group and the non-lymph node metastasis group ( Z=3.39, P=0.001, Z=-3.06, P=0.002); no statistical significance was found in the ADC and D values between two groups (both P>0.05). A total of 828 texture feature parameters were obtained based on T 2WI of primary lesion of rectal adenocarcinoma, among which 3 optimal texture feature parameters were selected, including firstorder_Skewness, shape_Sphericity and glcm_Idn. The ROC curve results showed that the AUC of D * and f were 0.689 and 0.670, respectively. The AUC of 3 texture feature parameters were 0.651, 0.628, 0.631, respectively. The AUC of texture feature model and the combined model were 0.775 and 0.803. The AUC of combined model was larger than D *, f and the three texture feature parameters (all P<0.05). Conclusion:IVIM-DWI parameters combined with T 2WI texture feature parameters in primary lesion of rectal adenocarcinoma show good diagnostic efficacy in preoperative prediction of lymph node metastasis with short diameter≤9 mm.

Objective:To investigate the antioxidant function of Mycoplasma pneumoniae MPN662 and analyze the key active sites, and to explore the role of MPN662 in the regulation of the production of reactive oxygen species (ROS) and superoxide dismutase (SOD) in THP-1 cells. Methods:pET28a(+ )- mpn662, recombinant mutant plasmids pET28a(+ )- mpn662-Ser 66 (the 66 th Cys was mutated to Ser) and pET28a(+ )- mpn662-Ala 66 (the 66 th Cys was mutated to Ala) were constructed, recombinant proteins rMPN662, rMPN662-Ser 66 and rMPN662-Ala 66 were expressed, identified, and purified. DTNB method was employed to analyze the MetO reduction activity of rMPN662 and recombinant mutant protein. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot were applied to examine the transcription level of the mpn662 gene and the expression level of MPN662 protein after Mycoplasma pneumoniae were stimulated with different concentrations of hydrogen peroxide (H 2O 2) or tert-butyl hydroperoxide (t-BHP), respectively. Fluorescent probes (DCFH-DA) and the total SOD activity detection kit were used to test the levels of intracellular ROS and SOD in THP-1 cells, which were pretreated with rMPN662, and then stimulated by Mycoplasma pneumoniae lipid-associated membrane proteins (LAMPs). Results:Mycoplasma pneumoniae rMPN662 could reduce MetO to Met, and the enzyme activities of mutant protein were significantly lower than those of rMPN662 protein. mpn662 gene mRNA transcription level and MPN662 protein expression level were significantly increased in a dose-dependent manner when Mycoplasma pneumoniae was stimulated with H 2O 2 and t-BHP. Treatment with rMPN662 before THP-1 cells were exposed to LAMPs could decrease the level of ROS and increase the production of SOD. Conclusions:Mycoplasma pneumoniae MPN662 can reduce MetO to Met, and Cys66 is the key amino acid for this activity. MPN662 can decrease the release of ROS and increase the production of SOD in Mycoplasma pneumoniae LAMPs stimulated THP-1 cells.

OBJECTIVE：To study the h ypoglycemic effect of Hordeum vulgare polysaccharide（HVP）on diabetes mellitus model mice and its mechanism. METHODS ：The mice were given intraperitoneal injection of streptozotocin （120 mg/kg）to induce diabetes mellitus model. After modeling ，the mice were randomly divided into model group ，metformin group （positive control ， 200 mg/kg），HVP high-dose ，medium-dose and low-dose groups （300，150 and 75 mg/kg），with 10 mice in each group. Blank control group was established additionally. Administration groups were given relevant medicine intragastrically ；blank control group and model group were given constant volume of water intragastrically ，once a day ，for consecutive 30 days. The levels of fasting blood glucose （FBG）were determined after 10，20，30 days of administration. After last FBG test ，mice were intraperitoneally injected with 10％ glucose solution （2 g/kg），then the area under the glucose tolerance （GTT）curve was measured at 30 and 120 min after injection. The serum levels of insulin ，the content of superoxide dismutase （SOD），glutathione peroxidase （GSH-Px）， malondialdehyde（MDA）in liver tissue were detected. Pancreatic morphology of mice were detected by HE staining. RESULTS ： Compared with blank control group ，the FBG ，area under GTT curve and MDA contnet in liver tissue were increased significantly in model group （P＜0.01），while serum levels of insulin ，SOD and GSH-Px contents in liver tissue were decreased significantly （P＜0.01）；the pancreatic tissue was seriously damaged with incomplete morphology and obvious vacuoles. Compared with model group，FBG，area under GTT curve ，MDA content in liver Δ 基金项目：国家自然科学基金资助项目（No.81473179） tissue were decreased significantly in HVP high-dose group ＊主管药师 。研究方向 ：临床药学 。电话：0510-85351490。E- mail：Wangshengya_helen@126.com （P＜0.05 or P＜0.01），serum content of insulin ，the content # 通信作者 ：副主任药师 。研究方向 ：医院药学 。电话：0510- of SOD and GSH-Px in liver tissue were increased 85351490。E-mail：3103221944@qq.com significantly（P＜0.05 or P＜0.01）；above indexes of HVP 中国药房 2021年第32卷第7期 China Pharmacy 2021Vol. 32 No. 7 ·807· low-dose and medium-dose groups were improved partially ，with statistical significance （P＜0.05 or P＜0.01）；the pancreatic tissue damage was alleviated ，the morphology was relatively complete ，the islet cells were closely arranged ，and the vacuoles were reduced. CONLUSIONS ：HVP can reduce the level of blood glucose in diabetic model mice ，increase insulin level and relieve pancreatic injury. The mechanism may be related to its antioxidation.